Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells

Year 2020, Volume: 79 Issue: 2, 157 - 162, 25.12.2020

Süleyman İlhan

Abstract

Objective: The aim of the study was to investigate the changes in the expression levels of apoptosis-related proteins after treatment with curcumin (Cur) on multiple drug-resistant H69AR non-small cell lung cancer cells. Materials and Methods: Viability of H69AR cells after Cur exposure (5-100 μg/mL) was evaluated via MTT assay at 24, 48 and 72 h. Apoptosis was assessed via ELISA assay. Apoptosis related proteins of breast cancer cell lines were analyzed by a Human Apoptosis Antibody Array. Protein-protein interactions were analyzed and visualized by using the STRING database. Results: Cur inhibited cell viability and induced apoptosis in H69AR cells. The IC50 value of Cur in H69AR cells was 8.75 μg/ mL. The array results showed that the protein levels of pro-apoptotic proteins such as Bad, Bax, Caspase-3, TRAIL R1, TRAIL R2, FADD, Fas, SMAC/DIABLO, HMOX2 were significantly increased by 2.4-, 3.1-, 2.6-, 3.1-, 3.4-, 2.4-, 2.1-, 4.1- and 5.5-fold in H69AR cells (p<0.05). Moreover, the protein levels of the anti-apoptotic proteins such as Bcl-2, cIAP-1, CLU and HIF1A were significantly decreased by 4.1-, 3.2-, 2.2- and 2.0-fold, respectively in H69AR cells by Cur exposure (p<0.05). Conclusion: Findings of this study suggested that Cur induced apoptosis of human H69AR cells via mediating several proteins involved in both extrinsic and intrinsic apoptotic pathways.

Keywords

Curcumin, apoptosis, protein array, protein-protein interactions

Supporting Institution

There are no funders to report for this submission.

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