Year 2020, Volume 79 , Issue 2, Pages 157 - 162 2020-12-25

Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells

Süleyman İLHAN [1]


Objective: The aim of the study was to investigate the changes in the expression levels of apoptosis-related proteins after treatment with curcumin (Cur) on multiple drug-resistant H69AR non-small cell lung cancer cells. Materials and Methods: Viability of H69AR cells after Cur exposure (5-100 μg/mL) was evaluated via MTT assay at 24, 48 and 72 h. Apoptosis was assessed via ELISA assay. Apoptosis related proteins of breast cancer cell lines were analyzed by a Human Apoptosis Antibody Array. Protein-protein interactions were analyzed and visualized by using the STRING database. Results: Cur inhibited cell viability and induced apoptosis in H69AR cells. The IC50 value of Cur in H69AR cells was 8.75 μg/ mL. The array results showed that the protein levels of pro-apoptotic proteins such as Bad, Bax, Caspase-3, TRAIL R1, TRAIL R2, FADD, Fas, SMAC/DIABLO, HMOX2 were significantly increased by 2.4-, 3.1-, 2.6-, 3.1-, 3.4-, 2.4-, 2.1-, 4.1- and 5.5-fold in H69AR cells (p<0.05). Moreover, the protein levels of the anti-apoptotic proteins such as Bcl-2, cIAP-1, CLU and HIF1A were significantly decreased by 4.1-, 3.2-, 2.2- and 2.0-fold, respectively in H69AR cells by Cur exposure (p<0.05). Conclusion: Findings of this study suggested that Cur induced apoptosis of human H69AR cells via mediating several proteins involved in both extrinsic and intrinsic apoptotic pathways.
Curcumin, apoptosis, protein array, protein-protein interactions
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Primary Language en
Subjects Science
Journal Section Research Articles
Authors

Orcid: 0000-0002-6584-3979
Author: Süleyman İLHAN (Primary Author)
Institution: MANISA CELAL BAYAR UNIVERSITY
Country: Turkey


Supporting Institution There are no funders to report for this submission.
Dates

Application Date : April 9, 2020
Acceptance Date : June 19, 2021
Publication Date : December 25, 2020

Bibtex @research article { iufsjb827075, journal = {European Journal of Biology}, issn = {2602-2575}, eissn = {2618-6144}, address = {}, publisher = {Istanbul University}, year = {2020}, volume = {79}, pages = {157 - 162}, doi = {}, title = {Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells}, key = {cite}, author = {İlhan, Süleyman} }
APA İlhan, S . (2020). Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells . European Journal of Biology , 79 (2) , 157-162 . Retrieved from https://dergipark.org.tr/en/pub/iufsjb/issue/55111/827075
MLA İlhan, S . "Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells" . European Journal of Biology 79 (2020 ): 157-162 <https://dergipark.org.tr/en/pub/iufsjb/issue/55111/827075>
Chicago İlhan, S . "Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells". European Journal of Biology 79 (2020 ): 157-162
RIS TY - JOUR T1 - Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells AU - Süleyman İlhan Y1 - 2020 PY - 2020 N1 - DO - T2 - European Journal of Biology JF - Journal JO - JOR SP - 157 EP - 162 VL - 79 IS - 2 SN - 2602-2575-2618-6144 M3 - UR - Y2 - 2021 ER -
EndNote %0 European Journal of Biology Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells %A Süleyman İlhan %T Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells %D 2020 %J European Journal of Biology %P 2602-2575-2618-6144 %V 79 %N 2 %R %U
ISNAD İlhan, Süleyman . "Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells". European Journal of Biology 79 / 2 (December 2020): 157-162 .
AMA İlhan S . Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells. Eur J Biol. 2020; 79(2): 157-162.
Vancouver İlhan S . Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells. European Journal of Biology. 2020; 79(2): 157-162.
IEEE S. İlhan , "Profile of Apoptotic Proteins After Curcumin Treatment by Antibody Array in H69AR Lung Cancer Cells", European Journal of Biology, vol. 79, no. 2, pp. 157-162, Dec. 2020