Year 2020, Volume 79 , Issue 2, Pages 115 - 123 2020-12-25

The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines

Caglar BERKEL [1] , Burak KUCUK [2] , Merve USTA [3] , Esra YILMAZ [4] , Ercan CACAN [5]


Objective: Ovarian cancer (OC) is the deadliest gynecologic malignancy and has a poor survival rate due to late diagnosis and chemoresistance development. In the standard treatment of OC, platinum-based chemotherapeutics are used. However, following several rounds of chemotherapy, these drugs’ efficacy eventually becomes limited due to the chemoresistance in most patients who previously responded to this treatment. Therefore, overcoming chemoresistance in the treatment of OC is of high importance. In this study, we investigated the effect of combinatorial inhibition of poly(ADP-ribose)polymerase (PARP) and proteasome by olaparib and bortezomib on chemosensitive and chemoresistant OC cell lines. Materials and Methods: We used sulphorhodamine B assay to screen cell viability following drug treatments alone or in combination, and used the cytotoxicity data to model the effect of drugs on cell death in R programming environment. In addition to olaparib and bortezomib, we performed cytotoxicity screenings where we applied cisplatin to OC cells. We also carried out flow cytometry analysis to quantify apoptotic cells following treatments. Results: We showed that combination treatment was more effective on chemosensitive OC cell lines when cisplatin was not used. In the presence of cisplatin, olaparib and bortezomib combination treatment resulted in higher cytotoxicity in chemoresistant OC lines compared to chemosensitive OC cell lines. Combinatorial inhibition of PARP and proteasome led to a higher number of apoptotic cells in OV2008 chemosensitive cell line compared to drugs alone. Conclusion: Our data shows that olaparib and bortezomib combination treatment might show promise in vivo in the treatment of OC. Also, the efficacy of this combination treatment might be dependent on OC cells’ chemosensitivity profiles.
Ovarian cancer, olaparib, bortezomib, chemoresistance, proteasome
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Primary Language en
Subjects Science
Journal Section Research Articles
Authors

Orcid: 0000-0003-4787-5157
Author: Caglar BERKEL
Institution: Tokat Gaziosmanpasa University
Country: Turkey


Orcid: 0000-0002-9325-2359
Author: Burak KUCUK
Institution: Tokat Gaziosmanpasa University
Country: Turkey


Orcid: 0000-0002-5086-6270
Author: Merve USTA
Institution: Tokat Gaziosmanpasa University
Country: Turkey


Orcid: 0000-0002-9922-8341
Author: Esra YILMAZ
Institution: Tokat Gaziosmanpasa University
Country: Turkey


Orcid: 0000-0002-3487-9493
Author: Ercan CACAN (Primary Author)
Institution: Tokat Gaziosmanpasa University
Country: Turkey


Supporting Institution The authors declared that this study has received no financial support.
Dates

Application Date : September 7, 2020
Acceptance Date : June 17, 2021
Publication Date : December 25, 2020

Bibtex @research article { iufsjb837603, journal = {European Journal of Biology}, issn = {2602-2575}, eissn = {2618-6144}, address = {}, publisher = {Istanbul University}, year = {2020}, volume = {79}, pages = {115 - 123}, doi = {}, title = {The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines}, key = {cite}, author = {Berkel, Caglar and Kucuk, Burak and Usta, Merve and Yılmaz, Esra and Cacan, Ercan} }
APA Berkel, C , Kucuk, B , Usta, M , Yılmaz, E , Cacan, E . (2020). The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines . European Journal of Biology , 79 (2) , 115-123 . Retrieved from https://dergipark.org.tr/en/pub/iufsjb/issue/55111/837603
MLA Berkel, C , Kucuk, B , Usta, M , Yılmaz, E , Cacan, E . "The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines" . European Journal of Biology 79 (2020 ): 115-123 <https://dergipark.org.tr/en/pub/iufsjb/issue/55111/837603>
Chicago Berkel, C , Kucuk, B , Usta, M , Yılmaz, E , Cacan, E . "The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines". European Journal of Biology 79 (2020 ): 115-123
RIS TY - JOUR T1 - The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines AU - Caglar Berkel , Burak Kucuk , Merve Usta , Esra Yılmaz , Ercan Cacan Y1 - 2020 PY - 2020 N1 - DO - T2 - European Journal of Biology JF - Journal JO - JOR SP - 115 EP - 123 VL - 79 IS - 2 SN - 2602-2575-2618-6144 M3 - UR - Y2 - 2021 ER -
EndNote %0 European Journal of Biology The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines %A Caglar Berkel , Burak Kucuk , Merve Usta , Esra Yılmaz , Ercan Cacan %T The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines %D 2020 %J European Journal of Biology %P 2602-2575-2618-6144 %V 79 %N 2 %R %U
ISNAD Berkel, Caglar , Kucuk, Burak , Usta, Merve , Yılmaz, Esra , Cacan, Ercan . "The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines". European Journal of Biology 79 / 2 (December 2020): 115-123 .
AMA Berkel C , Kucuk B , Usta M , Yılmaz E , Cacan E . The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines. Eur J Biol. 2020; 79(2): 115-123.
Vancouver Berkel C , Kucuk B , Usta M , Yılmaz E , Cacan E . The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines. European Journal of Biology. 2020; 79(2): 115-123.
IEEE C. Berkel , B. Kucuk , M. Usta , E. Yılmaz and E. Cacan , "The Effect of Olaparib and Bortezomib Combination Treatment on Ovarian Cancer Cell Lines", European Journal of Biology, vol. 79, no. 2, pp. 115-123, Dec. 2020