Research Article
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Year 2020, Volume: 79 Issue: 2, 67 - 74, 25.12.2020

Abstract

References

  • Kumar GP, Kumar R, Chaurasia OP, Singh SB. Current status and potential prospects of medicinal plant sector in trans-Himalayan Ladakh. J Med Plants Res 2011; 5: 2929-2940.
  • Bashir A. Antioxidant activity and phenolic compounds from Colchicum luteum Baker (Liliaceae). Afr J Biotechnol 2010; 9: 5762-5766.
  • Wechsler B. Colchicine and Behcet's disease: An efficiency enfinreconnue! [Behcet's disease and colchicines: An efficacious treatment finally recognized. J Med Int 2002; 23: 355-356.
  • Sakarkar DM, Deshmukh VN. Ethno pharmacological review of traditional medicinal plants for anticancer activity. Int J Pharm Tech Research 2011; 3: 298-308.
  • Azmat A, Ahmed KZ, Tariq B. Antinocciceptive effects of poly herbal oil extract. Pak J Pharmacy 2006; 23: 1-7.
  • Al-Fayyad M. Study of morphological characteristics, colchicine content and genetic variation of two wild Colchicum species in Jordan. Ph.D. Thesis, University of Jordan, Amman, Jordan. 2001.
  • Inchem. Colchicum autumnale L. Accessed on line at website: http://www.inchem. rg/documents/ ims/plant/ im142.htm. 2006; PP: 1-33.
  • Naz R, Anis M. Acceleration of adventitious shoots by interaction between exogenous hormone and adenine sulphate in Althaea officinalis L. Appl Biochem Biotechnol 2012; 168:1239–1255.
  • Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 1962; 15:473–497.
  • Morteza AN, Hossein A, Shamsali R, Mahmoud S, Yahya S, Navid VM. Warm stratification and chemical treatments overcome the dormancy and promotes germination of Colchicum kotschyi Boiss seeds under in vitro condition. Not Sci Biol 2011; 3:104-107.
  • Nidal QD, Rida AS, Ibrahim MM, Feras A, Tamara SA. Callus Culture and Somatic Embryogenesis in Wild Colchicum hierosolymitanum Feib. Jordan J Agricultural Sciences 2012; 8: 536-556.
  • Ling APK, Tan KP, Hussein S. Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata. J Zhejiang Univ Sci B 2013; 14: 621-631
  • Ghosh S, Sengupta S, Pal A. Abscisic acid, one of the key determinants of in vitro shoot differentiation from cotyledons of Vigna radiate. Amer J Pl Sci 2014; 5: 704 -713.
  • Su YH, Zhao XY, Liu YB, Zhang CL, O'Neill SD, Zhang X. Auxin-induced WUS expression is essential for embryonic stem cell renewal during somatic embryogenesis in Arabidopsis. Plant J 2009; 59: 448–460.
  • Feher A. Somatic embryogenesis - Stress-induced remodeling of plant cell fate Biochimicaet Biophysica Acta 2015; 1849: 385-402.
  • Arafeh RM, Shibli RA, Al-Mahmoud M, Shatnawi MA. Callusing, cell suspension culture and secondary metabolites production in Persian Organ (Origanum vulgare L.) and Arabian Oregano (O. syriacum L.). Jordan J Agri Sci 2006; 2: 274-282.
  • Shweta S, Madhuparna B, Haider ZA. Conservation of Colchicum luteum Baker through micropropagation. J Research (BAU) 2013; 25: 96-100.
  • Gao F, Yong Y, Dai C. Effects of endophytic fungal elicitor on two kinds of terpenoids production and physiological indexes in Euphorbia pekinensis suspension cells. J Med Plants Res 2011; 5: 4418- 4425.
  • Yang JL, Seong ES, Kim MJ, Ghimire BK, Kang WH, Yu CY, et al. Direct somatic embryogenesis from pericycle cells of broccoli (Brassica oleracea L. var. italica) root explants. Plant Cell Tiss Org Cult 2010; 100: 49-58.
  • Aslam J, Mujib A, Sharma MP. Somatic embryogenesis in Catharanthus roseus: a scanning electron microscopic study. Not Sci Biol 2015; 6 (2): 167-172.
  • Wang HC, Chen JT, Chang WC. Morphogenetic routes of long-term embryogenic callus culture of Areca catechu. Biol Plant 2010; 54:1–5.
  • Gliwicka M, Nowak K, Balazadeh S, Mueller-Roeber B, Gaj MD. Extensive modulation of the transcription factor transcriptome during somatic embryogenesis in Arabidopsis thaliana. PLoS ONE 2013; 8: e69261. doi: 10.1371/journal.pone.0069261.
  • Bai B, Su YH, Yuan J, Zhang XS. Induction of somatic embryos in Arabidopsis requires local YUCCA expression mediated by the down-regulation of ethylene biosynthesis. Mol Plant 2013; 6: 1247–1260.
  • Mujib A, Ali M, Dipti T, Zafar N. Embryogenesis in ornamental monocots: Plant growth regulators as signaling element. In: Mujib, A., (ed). Somatic embryogenesis in ornamentals and its applications, Springer 2016 pp: 187-201.
  • Ashraf MF. Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. &Fernandez. Electronic J Biotech 2014; 7: 275-279.
  • Ramakrishna A, Ravishankar GA. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 2011; 6: 1720-1731.
  • Ribkahwati Purnobasuki H, Isnaeni Utami ESW. Quantity essential oil from rose callus leaf (Rosa hybrid L. variety Hybride tea purple): Results of light elicitation. J Chem Pharma Res 2015; 7: 496- 499.
  • Samar F, Mujib A, Tonk D. NaCl amendment improves vinblastine and vincristine synthesis in Catharanthus roseus: a case of stress signalling as evidenced by antioxidant enzymes activities. Plant Cell Tissue Org Cult 2015; 121: 445- 458.
  • Al-Fayyad M, Alali F, Alkofali A, Tell A. Determination of colchicine content in Colchicum hierosolymitanum and Colchicum tunicatum under cultivation. Nat Prod Let 2002; 16: 395-400.
  • Daradkeh NQ, Shibli RA, Makhadmeh IM, Alali F, Al-Qudah TS. Cell suspension and in vitro production of colchicine in wild colchicum hierosolymitanum Feib. TOPROCJ (2012); 3: 52–59. 10.2174/1876326X01203020052.
  • Sivakumar G, Alba K, Phillips GC. Biorhizome: A Biosynthetic Platform for Colchicine Biomanufacturing. Front Plant Sci 2017; 8: 1137.
  • Tatiana YG , Valeria PG, Dmitry VB, Galina KT, Victor PB. Tempo-spatial pattern of stepharine accumulation in Stephania glabra morphogenic tissues. Int J Mol Sci 2019; 20: 808, doi:10.3390/ijms20040808.

Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography

Year 2020, Volume: 79 Issue: 2, 67 - 74, 25.12.2020

Abstract

Objective: The aim of the present work was to develop a protocol for season independent propagation of Colchicum luteum and enrichment of colchicine yield under salt stress.
Materials and Methods: C. luteum was collected from Kashmir, India and was cultured in vitro. The callus was exposed to different NaCl (salt) treatments and the yield of colchicine was quantified by high pressure liquid chromatography (HPLC). Results: Different explants viz. seeds, leaves, anthers and corm were used and the callus was only induced from corm
segments. The callus induction and proliferation were best achieved on Murashige and Skoog medium supplemented with 2.0 mg L-1 2, 4-D + 4.0 mg L-1 BAP. Direct and indirect plant regeneration from corm was observed in 2.0 mg L-1 BAP + 2.0 mg L-1 NAA added medium. The addition of adjuvants like activated charcoal and citric acid was noted to be less efficient for
improving callus growth. Induced callus was elicitated with different NaCl concentrations (T0 = without NaCl, T1 = 25, T2 = 50, T3 = 75, and T4 =100 mM). The yield of colchicine was quantified by HPLC at periodic intervals (2, 4, and 6 weeks). All the used levels improved colchicine yield, but the content was maximum at T3 (871.4 ng mg-1 DWB). This is the first report of callus induction and plant regeneration from corm and the quantification of colchicine under salt stress in C. luteum. Conclusion: Sodium chloride is thus a potential inducer and elicitor in improving colchicine yield in C. luteum.

Supporting Institution

The authors declared that this study has received no financial support.

References

  • Kumar GP, Kumar R, Chaurasia OP, Singh SB. Current status and potential prospects of medicinal plant sector in trans-Himalayan Ladakh. J Med Plants Res 2011; 5: 2929-2940.
  • Bashir A. Antioxidant activity and phenolic compounds from Colchicum luteum Baker (Liliaceae). Afr J Biotechnol 2010; 9: 5762-5766.
  • Wechsler B. Colchicine and Behcet's disease: An efficiency enfinreconnue! [Behcet's disease and colchicines: An efficacious treatment finally recognized. J Med Int 2002; 23: 355-356.
  • Sakarkar DM, Deshmukh VN. Ethno pharmacological review of traditional medicinal plants for anticancer activity. Int J Pharm Tech Research 2011; 3: 298-308.
  • Azmat A, Ahmed KZ, Tariq B. Antinocciceptive effects of poly herbal oil extract. Pak J Pharmacy 2006; 23: 1-7.
  • Al-Fayyad M. Study of morphological characteristics, colchicine content and genetic variation of two wild Colchicum species in Jordan. Ph.D. Thesis, University of Jordan, Amman, Jordan. 2001.
  • Inchem. Colchicum autumnale L. Accessed on line at website: http://www.inchem. rg/documents/ ims/plant/ im142.htm. 2006; PP: 1-33.
  • Naz R, Anis M. Acceleration of adventitious shoots by interaction between exogenous hormone and adenine sulphate in Althaea officinalis L. Appl Biochem Biotechnol 2012; 168:1239–1255.
  • Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 1962; 15:473–497.
  • Morteza AN, Hossein A, Shamsali R, Mahmoud S, Yahya S, Navid VM. Warm stratification and chemical treatments overcome the dormancy and promotes germination of Colchicum kotschyi Boiss seeds under in vitro condition. Not Sci Biol 2011; 3:104-107.
  • Nidal QD, Rida AS, Ibrahim MM, Feras A, Tamara SA. Callus Culture and Somatic Embryogenesis in Wild Colchicum hierosolymitanum Feib. Jordan J Agricultural Sciences 2012; 8: 536-556.
  • Ling APK, Tan KP, Hussein S. Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata. J Zhejiang Univ Sci B 2013; 14: 621-631
  • Ghosh S, Sengupta S, Pal A. Abscisic acid, one of the key determinants of in vitro shoot differentiation from cotyledons of Vigna radiate. Amer J Pl Sci 2014; 5: 704 -713.
  • Su YH, Zhao XY, Liu YB, Zhang CL, O'Neill SD, Zhang X. Auxin-induced WUS expression is essential for embryonic stem cell renewal during somatic embryogenesis in Arabidopsis. Plant J 2009; 59: 448–460.
  • Feher A. Somatic embryogenesis - Stress-induced remodeling of plant cell fate Biochimicaet Biophysica Acta 2015; 1849: 385-402.
  • Arafeh RM, Shibli RA, Al-Mahmoud M, Shatnawi MA. Callusing, cell suspension culture and secondary metabolites production in Persian Organ (Origanum vulgare L.) and Arabian Oregano (O. syriacum L.). Jordan J Agri Sci 2006; 2: 274-282.
  • Shweta S, Madhuparna B, Haider ZA. Conservation of Colchicum luteum Baker through micropropagation. J Research (BAU) 2013; 25: 96-100.
  • Gao F, Yong Y, Dai C. Effects of endophytic fungal elicitor on two kinds of terpenoids production and physiological indexes in Euphorbia pekinensis suspension cells. J Med Plants Res 2011; 5: 4418- 4425.
  • Yang JL, Seong ES, Kim MJ, Ghimire BK, Kang WH, Yu CY, et al. Direct somatic embryogenesis from pericycle cells of broccoli (Brassica oleracea L. var. italica) root explants. Plant Cell Tiss Org Cult 2010; 100: 49-58.
  • Aslam J, Mujib A, Sharma MP. Somatic embryogenesis in Catharanthus roseus: a scanning electron microscopic study. Not Sci Biol 2015; 6 (2): 167-172.
  • Wang HC, Chen JT, Chang WC. Morphogenetic routes of long-term embryogenic callus culture of Areca catechu. Biol Plant 2010; 54:1–5.
  • Gliwicka M, Nowak K, Balazadeh S, Mueller-Roeber B, Gaj MD. Extensive modulation of the transcription factor transcriptome during somatic embryogenesis in Arabidopsis thaliana. PLoS ONE 2013; 8: e69261. doi: 10.1371/journal.pone.0069261.
  • Bai B, Su YH, Yuan J, Zhang XS. Induction of somatic embryos in Arabidopsis requires local YUCCA expression mediated by the down-regulation of ethylene biosynthesis. Mol Plant 2013; 6: 1247–1260.
  • Mujib A, Ali M, Dipti T, Zafar N. Embryogenesis in ornamental monocots: Plant growth regulators as signaling element. In: Mujib, A., (ed). Somatic embryogenesis in ornamentals and its applications, Springer 2016 pp: 187-201.
  • Ashraf MF. Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. &Fernandez. Electronic J Biotech 2014; 7: 275-279.
  • Ramakrishna A, Ravishankar GA. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 2011; 6: 1720-1731.
  • Ribkahwati Purnobasuki H, Isnaeni Utami ESW. Quantity essential oil from rose callus leaf (Rosa hybrid L. variety Hybride tea purple): Results of light elicitation. J Chem Pharma Res 2015; 7: 496- 499.
  • Samar F, Mujib A, Tonk D. NaCl amendment improves vinblastine and vincristine synthesis in Catharanthus roseus: a case of stress signalling as evidenced by antioxidant enzymes activities. Plant Cell Tissue Org Cult 2015; 121: 445- 458.
  • Al-Fayyad M, Alali F, Alkofali A, Tell A. Determination of colchicine content in Colchicum hierosolymitanum and Colchicum tunicatum under cultivation. Nat Prod Let 2002; 16: 395-400.
  • Daradkeh NQ, Shibli RA, Makhadmeh IM, Alali F, Al-Qudah TS. Cell suspension and in vitro production of colchicine in wild colchicum hierosolymitanum Feib. TOPROCJ (2012); 3: 52–59. 10.2174/1876326X01203020052.
  • Sivakumar G, Alba K, Phillips GC. Biorhizome: A Biosynthetic Platform for Colchicine Biomanufacturing. Front Plant Sci 2017; 8: 1137.
  • Tatiana YG , Valeria PG, Dmitry VB, Galina KT, Victor PB. Tempo-spatial pattern of stepharine accumulation in Stephania glabra morphogenic tissues. Int J Mol Sci 2019; 20: 808, doi:10.3390/ijms20040808.
There are 32 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Mehpara Maqsood This is me 0000-0002-0966-2946

Mir Khusrau This is me

Zahoor A. Kaloo This is me 0000-0002-1968-476X

Tariq A. Wanı This is me 0000-0002-1530-7027

Abdul Mujıb This is me

Publication Date December 25, 2020
Submission Date April 8, 2020
Published in Issue Year 2020 Volume: 79 Issue: 2

Cite

AMA Maqsood M, Khusrau M, Kaloo ZA, Wanı TA, Mujıb A. Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography. Eur J Biol. December 2020;79(2):67-74.