Research Article
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Antioxidant, Cytotoxic, and Enzyme Inhibitory Activities of Agropyron repens and Crataegus monogyna Species

Year 2020, Volume: 79 Issue: 2, 98 - 105, 25.12.2020

Abstract

Objective: The aim of this study was to investigate antioxidant, enzyme inhibitory and cytotoxic activities of Agropyron repens and Crataegus monogyna methanol extracts with total phenolic and flavonoid contents. Materials and Methods: Total phenolic and flavonoid contents of A. repens and C. monogyna methanol extracts were measured according to Folin Ciocalteu and aluminum nitrate methods, respectively. Antioxidant and enzyme inhibitory activities of the methanol extracts were tested spectrophotometrically. Also, cytotoxic activities of the methanol extracts
against DLD-1 and CCD-18Co were investigated by using Alamar Blue assay. Results: C. monogyna methanol extract with the highest total phenolic and flavonoid contents (68.13±0.34 μg GAEs/mg extract and 36.91±0.17 μg QEs/mg extract, respectively) had the best antioxidant activity in β-carotene-linoleic acid (IC50: 32.72±0.15 μg/mL), CUPRAC (A0.50: 282.69±0.25 μg/mL), DPPH• (IC50: 71.69±0.85 μg/mL), and ABTS•+ (IC50: 40.43±0.55 μg/ mL) assays. A. repens methanol extract showed the highest effect against AChE (18.73±0.47 %), BChE (37.59±1.07 %), urease (89.18±0.84%), α-glucosidase (6.71±0.23 %), whereas C. monogyna methanol extract showed the highest effect against tyrosinase (30.52±1.00%) and α-amylase (37.24±0.06 %). Also, A. repens (IC50: 57.38 μg/mL) and C. monogyna (IC50: 54.04 μg/ mL) methanol extracts showed close cytotoxic activity on DLD-1. Conclusion: Antioxidant, cytotoxic, and enzyme inhibitory activities of A. repens and C. monogyna methanol extracts were investigated with total phenolic and flavonoid contents in this study. The results obtained with this study strengthen the potential of the studied plants as a new source for the discovery of antioxidant, cytotoxic, and enzyme inhibitor agents.

Supporting Institution

There are no funders to report for this submission.

Thanks

Authors would like to thank Dr. Ergün Kaya (Faculty of Science, Department of Molecular Biology and Genetics, Muğla Sıtkı Koçman University) for the identification of the plant samples.

References

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  • 2. Top R, Erden Y, Tekin S. The investigation of antioxidant and anticancer effects of some importance medical plants. BEU J Sci 2019; 8(2):435-42.
  • 3. Ginwala R, Bhavsar R, Chigbu DI, Jain P, Khan ZK. Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the anti-inflammatory activity of apigenin. Antioxidants 2019; 8(2):e35.
  • 4. Rawat D, Shrivastava S, Naik RA, Chhonker SK, Mehrotra A, Koiri RK. An overview of natural plant products in the treatment of hepatocellular carcinoma. Anticancer Agents Med Chem 2018; 18(3):1838-59.
  • 5. Ferysiuk K, Wójciak KM. The spectrophotometric analysis of antioxidant properties of selected herbs in vision-pro™ uv-vis. Appl Comput Sci 2019; 15:49-62.
  • 6. Leporatti ML, Ivancheva S. Preliminary comparative analysis of medicinal plants used in the traditional medicine of Bulgaria and Italy. J Ethnopharmacol 2003; 87:123- 42.
  • 7. Mustafa B, Hajdari A, Krasniqi F, Hoxha E, Ademi H, Quave CL, et al. Medical etnobotany of the Albanian Alps in Kosovo. J Ethnobiol Ethnomed 2012; 8:6.
  • 8. Sargın SA, Akçicek E, Selvi S. An ethnobotanical study of medicinal plants used by the local people of Alaşehir (Manisa) in Turkey. J Ethnopharmacol 2013; 150:860-74.
  • 9. Grases F, Ramis M, Costa-Bauza A, March JG. Effect of Herniaria hirsuta and Agropyron repens on calcium oxalate urolithiasis risk in rats. J Ethnopharmacol 1995; 45:211-4.
  • 10. Mascolo N, Autore G, Capasso F, Menghini A, Fasulo MP. Biological screening of Italian medicinal plants for anti-inflammatory activity. Phytother Res 1987; 1:28-31.
  • 11. Rafsanjany N, Lechtenberg M, Petereit F, Hensel A. Antiadhesion as a functional concept for protection against uropathogenic Escherichia coli: In vitro studies with traditionally used plants with antiadhesive activity against uropathognic Escherichia coli. J Ethnopharmacol 2013; 145:591-7.
  • 12. Bardakci H, Celep E, Gözet T, Kan Y, Kırmızıbekmez H. Phytochemical characterization and antioxidant activities of the fruit extracts of several Crataegus taxa. S Afr J Bot 2019; 124:5-13.
  • 13. Edwards JE, Brown PN, Talent N, Dickinson TA, Shipley PR. A review of the chemistry of the genus Crataegus. Phytochemistry 2012; 79:5-26.
  • 14. Venskutonis PR. Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): a review of recent research advances. J Food Bioact 2018; 4:69-87.
  • 15. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Vitic 1977; 28:49-55.
  • 16. Park YK, Koo MH, Ikegaki M, Contado JL. Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil. Braz Arch Biol Technol 1997; 40:97-106.
  • 17. Çayan F, Tel-Çayan G, Deveci E, Öztürk M, Duru ME. Chemical profile, in vitro enzyme inhibitory, and antioxidant properties of Stereum species (Agaricomycetes) from Turkey. Int J Med Mushrooms 2019; 21(11):1075-87. 18. Deveci E, Tel‐Çayan G, Duru ME, Öztürk M. Phytochemical contents, antioxidant effects, and inhibitory activities of key enzymes associated with Alzheimer’s disease, ulcer, and skin disorders of Sideritis albiflora and Sideritis leptoclada. J Food Biochem 2019; 43:e13078.
  • 19. Deveci E, Tel Cayan G, Duru ME. In vitro antidiabetic activity of seven medicinal plants naturally growing in Turkey. Eur J Biol 2020; 79:23-8.
  • 20. Karakurt S, Adali O. Tannic acid inhibits proliferation, migration, invasion of prostate cancer and modulates drug metabolizing and antioxidant enzymes. Anticancer Agents Med Chem 2016; 16(6):781-9.
  • 21. Patch CS, Sullivan DR, Fenech M. Health benefits of herbs and spices: Cardiovascular disease. Med J Aust 2006; 185(4):7-9.
  • 22. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2010; 2(12):1231-46.
  • 23. Ignat I, Volf I, Popa VI. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem 2011; 126:1821-35.
  • 24. Tanase C, Cosarca S, Muntean DL. A critical review of phenolic compounds extracted from the bark of woody vascular plants and their potential biological activity. Molecules 2019; 24(6):1182.
  • 25. Karak P. Biological activities of flavonoids: an overview. Int J Pharm Sci Res 2019; 10(4):1567-74.
  • 26. Öztürk N, Tunçel M. Assessment of phenolic acid content and in vitro antiradical characteristics of hawthorn. J Med Food 2011; 14(6):664-9.
  • 27. Cosmulescu S, Trandafir I, Nour V. Phenolic acids and flavonoids profiles of extracts from edible wild fruits and their antioxidant properties. Int J Food Prop 2017; 20(12):3124-34.
  • 28. Dogan S, Diken ME, Dogan M. Antioxidant, phenolic and protein contents of some medicinal plants. J Med Plants Res 2010; 4(23):2566-73.
  • 29. Lukyanova LD, Storozheva ZI, Proshin AT. Corrective effect of flavonoid containing preparation extralife on the development of Parkinson's syndrome. Bull Exp Biol Med 2007; 144:42-5.
  • 30. Ghasemzadeh A, Ghasemzadeh N. Flavonoids and phenolic acids: Role and biochemical activity in plants and human. J Med Plants Res 2011; 5(31):6697-703.
  • 31. Lucio M, Nunes C, Gaspar D, Ferreira H, Lima JLFC, Reis S. Antioxidant activity of vitamin E and Trolox: understanding of the factors that govern lipid peroxidation studies in vitro. Food Biophys 2009; 4:312-20.
  • 32. Nunes R, Pasko P, Tyszka-Czochara M, Szewczyk A, Szlosarczyk M, Carvalho IS. Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs. Pharm Biol 2016; 55(1):114-23.
  • 33. Rocchetti G, Senizza B, Zengin G, Mahomodally MF, Senkardes I, Lobine D, et al. Untargeted metabolomic profiling of three Crataegus species (hawthorn) and their in vitro biological activities. J Sci Food Agric 2020; 100(5):1998-2006.
  • 34. Parzhanova AB, Petkova N, Ivanov IG, Ivanova SD. Evaluation of biologically active substance and antioxidant potential of medicinal plants extracts for food and cosmetic purposes. J Pharm Sci Res 2018; 10(7):1804-9.
  • 35. Rostami S. M.Sc Thesis. Investigation of antiproliferative and antiinflammatory effects of common and endemic species of plants. Department of Biology, Institute of Science, Gazi University, Ankara, Turkey, 2013.
  • 36. Doğan M, Akbulut H. Adjuvant Treatment of Colorectal Cancer. Turkiye Klinikleri J Med Oncol 2009; 2(3):49-57.
  • 37. Dellabona P, Moro M, Crosti MC, Casorati G, Corti A. Vascular attack and immunotherapy: a ‘two hits’ approach to improve biological treatment of cancer. Gene Ther 1999; 6:153-4.
  • 38. Terrero MN, Li S. Growth factor receptors: targets for gene therapy and immunotherapy for cancer treatment. Gene Ther Mol Biol 2004; 8:175-80.
  • 39. Ma L, Xu GB, Tang X, Zhang C, Zhao W, Wang J, et al. Anti-cancer potential of polysaccharide extracted from hawthorn (Crataegus.) on human colon cancer cell line HCT116 via cell cycle arrest and apoptosis. J Funct Foods 2020; 64:103677.
  • 40. Ganie SA, Dar TA, Zargar S, Bhat AH, Dar KB, Masood A, et al. Crataegus songarica methanolic extract accelerates enzymatic status in kidney and heart tissue damage in albino rats and its in vitro cytotoxic activity. Pharm Biol 2015; 54:1246-54.
  • 41. Howes MJR, Houghton PJ, Perry NSL. Plants wth tradtonal uses and actvtes, relevant to the management of Alzhemer's dsease and other cogntve dsorders. Phytother Res 2003; 17(1):1-18.
  • 42. Alkan HÖ, Zengin G, Kaşık G. Antioxidant and in vitro some enzyme inhibitory activities of methanolic extract of cultivated Lentinula edodes. J Fungus 2017; 8(2):90-8.
  • 43. Chang TM. Tyrosinase and tyrosinase inhibitors. J Biocatal Biotransformation 2012; 1:2.
  • 44. Gholamhoseinian A, ZohreRazmi Z. Screening the methanolic extracts of some plants for tyrosinase inhibitory activity. Toxicol Environ Chem 2012; 94:310-8.
  • 45. Baltas N, Karaoglu S, Tarakcı C, Kolayli S. Effect of propolis in gastric disorders: inhibition studies on the growth of Helicobacter pylori and production of its urease. J Enzyme Inhib Med Chem 2016; 31(S2):46-50.
  • 46. Baltas N, Yildiz O, Kolayli S. Inhibition properties of propolis extracts to some clinically important enzymes. J Enzyme Inhib Med Chem 2016; 31:52-5.
  • 47. Taslimi P, Gulçin İ. Antidiabetic potential: in vitro inhibition effects of some natural phenolic compounds on α-glycosidase and α-amylase enzymes. J Biochem Mol Toxicol 2017; 31(10):e21956.
  • 48. Kumar S, Kumar V, Rana M, Kumar D. Enzymes inhibitors from plants: An alternate approach to treat diabetes. Phcog Commn 2012; 2(2):18-33.
  • 49. Natić M, Pavlović A, Bosco FL, Stanisavljević N, Zagorac DD, Akšić MF, et al. Nutraceutical properties and phytochemical characterization of wild Serbian fruits. Eur Food Res Technol 2019; 245:469-78.
  • 50. Nowicka P, Wojdyło A. Anti-hyperglycemic and anticholinergic effects of natural antioxidant contents in edible flowers. Antioxidants 2019; 8:308.
Year 2020, Volume: 79 Issue: 2, 98 - 105, 25.12.2020

Abstract

References

  • 1. Dar RA, Shahnawaz M, Qazi PH. General overview of medicinal plants: A review. J Phytopharmacol 2017; 6:349-51.
  • 2. Top R, Erden Y, Tekin S. The investigation of antioxidant and anticancer effects of some importance medical plants. BEU J Sci 2019; 8(2):435-42.
  • 3. Ginwala R, Bhavsar R, Chigbu DI, Jain P, Khan ZK. Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the anti-inflammatory activity of apigenin. Antioxidants 2019; 8(2):e35.
  • 4. Rawat D, Shrivastava S, Naik RA, Chhonker SK, Mehrotra A, Koiri RK. An overview of natural plant products in the treatment of hepatocellular carcinoma. Anticancer Agents Med Chem 2018; 18(3):1838-59.
  • 5. Ferysiuk K, Wójciak KM. The spectrophotometric analysis of antioxidant properties of selected herbs in vision-pro™ uv-vis. Appl Comput Sci 2019; 15:49-62.
  • 6. Leporatti ML, Ivancheva S. Preliminary comparative analysis of medicinal plants used in the traditional medicine of Bulgaria and Italy. J Ethnopharmacol 2003; 87:123- 42.
  • 7. Mustafa B, Hajdari A, Krasniqi F, Hoxha E, Ademi H, Quave CL, et al. Medical etnobotany of the Albanian Alps in Kosovo. J Ethnobiol Ethnomed 2012; 8:6.
  • 8. Sargın SA, Akçicek E, Selvi S. An ethnobotanical study of medicinal plants used by the local people of Alaşehir (Manisa) in Turkey. J Ethnopharmacol 2013; 150:860-74.
  • 9. Grases F, Ramis M, Costa-Bauza A, March JG. Effect of Herniaria hirsuta and Agropyron repens on calcium oxalate urolithiasis risk in rats. J Ethnopharmacol 1995; 45:211-4.
  • 10. Mascolo N, Autore G, Capasso F, Menghini A, Fasulo MP. Biological screening of Italian medicinal plants for anti-inflammatory activity. Phytother Res 1987; 1:28-31.
  • 11. Rafsanjany N, Lechtenberg M, Petereit F, Hensel A. Antiadhesion as a functional concept for protection against uropathogenic Escherichia coli: In vitro studies with traditionally used plants with antiadhesive activity against uropathognic Escherichia coli. J Ethnopharmacol 2013; 145:591-7.
  • 12. Bardakci H, Celep E, Gözet T, Kan Y, Kırmızıbekmez H. Phytochemical characterization and antioxidant activities of the fruit extracts of several Crataegus taxa. S Afr J Bot 2019; 124:5-13.
  • 13. Edwards JE, Brown PN, Talent N, Dickinson TA, Shipley PR. A review of the chemistry of the genus Crataegus. Phytochemistry 2012; 79:5-26.
  • 14. Venskutonis PR. Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): a review of recent research advances. J Food Bioact 2018; 4:69-87.
  • 15. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Vitic 1977; 28:49-55.
  • 16. Park YK, Koo MH, Ikegaki M, Contado JL. Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil. Braz Arch Biol Technol 1997; 40:97-106.
  • 17. Çayan F, Tel-Çayan G, Deveci E, Öztürk M, Duru ME. Chemical profile, in vitro enzyme inhibitory, and antioxidant properties of Stereum species (Agaricomycetes) from Turkey. Int J Med Mushrooms 2019; 21(11):1075-87. 18. Deveci E, Tel‐Çayan G, Duru ME, Öztürk M. Phytochemical contents, antioxidant effects, and inhibitory activities of key enzymes associated with Alzheimer’s disease, ulcer, and skin disorders of Sideritis albiflora and Sideritis leptoclada. J Food Biochem 2019; 43:e13078.
  • 19. Deveci E, Tel Cayan G, Duru ME. In vitro antidiabetic activity of seven medicinal plants naturally growing in Turkey. Eur J Biol 2020; 79:23-8.
  • 20. Karakurt S, Adali O. Tannic acid inhibits proliferation, migration, invasion of prostate cancer and modulates drug metabolizing and antioxidant enzymes. Anticancer Agents Med Chem 2016; 16(6):781-9.
  • 21. Patch CS, Sullivan DR, Fenech M. Health benefits of herbs and spices: Cardiovascular disease. Med J Aust 2006; 185(4):7-9.
  • 22. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2010; 2(12):1231-46.
  • 23. Ignat I, Volf I, Popa VI. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem 2011; 126:1821-35.
  • 24. Tanase C, Cosarca S, Muntean DL. A critical review of phenolic compounds extracted from the bark of woody vascular plants and their potential biological activity. Molecules 2019; 24(6):1182.
  • 25. Karak P. Biological activities of flavonoids: an overview. Int J Pharm Sci Res 2019; 10(4):1567-74.
  • 26. Öztürk N, Tunçel M. Assessment of phenolic acid content and in vitro antiradical characteristics of hawthorn. J Med Food 2011; 14(6):664-9.
  • 27. Cosmulescu S, Trandafir I, Nour V. Phenolic acids and flavonoids profiles of extracts from edible wild fruits and their antioxidant properties. Int J Food Prop 2017; 20(12):3124-34.
  • 28. Dogan S, Diken ME, Dogan M. Antioxidant, phenolic and protein contents of some medicinal plants. J Med Plants Res 2010; 4(23):2566-73.
  • 29. Lukyanova LD, Storozheva ZI, Proshin AT. Corrective effect of flavonoid containing preparation extralife on the development of Parkinson's syndrome. Bull Exp Biol Med 2007; 144:42-5.
  • 30. Ghasemzadeh A, Ghasemzadeh N. Flavonoids and phenolic acids: Role and biochemical activity in plants and human. J Med Plants Res 2011; 5(31):6697-703.
  • 31. Lucio M, Nunes C, Gaspar D, Ferreira H, Lima JLFC, Reis S. Antioxidant activity of vitamin E and Trolox: understanding of the factors that govern lipid peroxidation studies in vitro. Food Biophys 2009; 4:312-20.
  • 32. Nunes R, Pasko P, Tyszka-Czochara M, Szewczyk A, Szlosarczyk M, Carvalho IS. Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs. Pharm Biol 2016; 55(1):114-23.
  • 33. Rocchetti G, Senizza B, Zengin G, Mahomodally MF, Senkardes I, Lobine D, et al. Untargeted metabolomic profiling of three Crataegus species (hawthorn) and their in vitro biological activities. J Sci Food Agric 2020; 100(5):1998-2006.
  • 34. Parzhanova AB, Petkova N, Ivanov IG, Ivanova SD. Evaluation of biologically active substance and antioxidant potential of medicinal plants extracts for food and cosmetic purposes. J Pharm Sci Res 2018; 10(7):1804-9.
  • 35. Rostami S. M.Sc Thesis. Investigation of antiproliferative and antiinflammatory effects of common and endemic species of plants. Department of Biology, Institute of Science, Gazi University, Ankara, Turkey, 2013.
  • 36. Doğan M, Akbulut H. Adjuvant Treatment of Colorectal Cancer. Turkiye Klinikleri J Med Oncol 2009; 2(3):49-57.
  • 37. Dellabona P, Moro M, Crosti MC, Casorati G, Corti A. Vascular attack and immunotherapy: a ‘two hits’ approach to improve biological treatment of cancer. Gene Ther 1999; 6:153-4.
  • 38. Terrero MN, Li S. Growth factor receptors: targets for gene therapy and immunotherapy for cancer treatment. Gene Ther Mol Biol 2004; 8:175-80.
  • 39. Ma L, Xu GB, Tang X, Zhang C, Zhao W, Wang J, et al. Anti-cancer potential of polysaccharide extracted from hawthorn (Crataegus.) on human colon cancer cell line HCT116 via cell cycle arrest and apoptosis. J Funct Foods 2020; 64:103677.
  • 40. Ganie SA, Dar TA, Zargar S, Bhat AH, Dar KB, Masood A, et al. Crataegus songarica methanolic extract accelerates enzymatic status in kidney and heart tissue damage in albino rats and its in vitro cytotoxic activity. Pharm Biol 2015; 54:1246-54.
  • 41. Howes MJR, Houghton PJ, Perry NSL. Plants wth tradtonal uses and actvtes, relevant to the management of Alzhemer's dsease and other cogntve dsorders. Phytother Res 2003; 17(1):1-18.
  • 42. Alkan HÖ, Zengin G, Kaşık G. Antioxidant and in vitro some enzyme inhibitory activities of methanolic extract of cultivated Lentinula edodes. J Fungus 2017; 8(2):90-8.
  • 43. Chang TM. Tyrosinase and tyrosinase inhibitors. J Biocatal Biotransformation 2012; 1:2.
  • 44. Gholamhoseinian A, ZohreRazmi Z. Screening the methanolic extracts of some plants for tyrosinase inhibitory activity. Toxicol Environ Chem 2012; 94:310-8.
  • 45. Baltas N, Karaoglu S, Tarakcı C, Kolayli S. Effect of propolis in gastric disorders: inhibition studies on the growth of Helicobacter pylori and production of its urease. J Enzyme Inhib Med Chem 2016; 31(S2):46-50.
  • 46. Baltas N, Yildiz O, Kolayli S. Inhibition properties of propolis extracts to some clinically important enzymes. J Enzyme Inhib Med Chem 2016; 31:52-5.
  • 47. Taslimi P, Gulçin İ. Antidiabetic potential: in vitro inhibition effects of some natural phenolic compounds on α-glycosidase and α-amylase enzymes. J Biochem Mol Toxicol 2017; 31(10):e21956.
  • 48. Kumar S, Kumar V, Rana M, Kumar D. Enzymes inhibitors from plants: An alternate approach to treat diabetes. Phcog Commn 2012; 2(2):18-33.
  • 49. Natić M, Pavlović A, Bosco FL, Stanisavljević N, Zagorac DD, Akšić MF, et al. Nutraceutical properties and phytochemical characterization of wild Serbian fruits. Eur Food Res Technol 2019; 245:469-78.
  • 50. Nowicka P, Wojdyło A. Anti-hyperglycemic and anticholinergic effects of natural antioxidant contents in edible flowers. Antioxidants 2019; 8:308.
There are 49 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Ebru Devecı This is me 0000-0002-2597-9898

Gulsen Tel Cayan This is me 0000-0002-1916-7391

Serdar Karakurt This is me 0000-0002-4449-6103

Mehmet Emin Duru This is me 0000-0001-7252-4880

Publication Date December 25, 2020
Submission Date October 27, 2020
Published in Issue Year 2020 Volume: 79 Issue: 2

Cite

AMA Devecı E, Tel Cayan G, Karakurt S, Duru ME. Antioxidant, Cytotoxic, and Enzyme Inhibitory Activities of Agropyron repens and Crataegus monogyna Species. Eur J Biol. December 2020;79(2):98-105.