Research Article
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Antibiofilm and Anti-Quorum Sensing Activities of Vaginal Origin Probiotics

Year 2021, Volume: 80 Issue: 2, 82 - 90, 17.12.2021
https://doi.org/10.26650/EurJBiol.2021.932640

Abstract

Objective: Multidrug-resistant bacteria generally use cell-to-cell communication that leads to biofilm formation as a resistance development mechanism. Some pathogenic bacteria can form biofilms through a mechanism called Quorum sensing (QS). QS inhibition is one of the effective approaches to prevent biofilm formation.

Materials and Methods: 20 Lactic acid bacteria (LAB) previously associated with identification by 16S rRNA sequence analysis were used. Antibiofilm activities of metabolites of strains related to microplate based Antibiofilm method on Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. Chromobacterium violaceum ATCC 12472 was used as an indicator in the anti-QS activities of LAB. The study was also performed by ELISA test on the immunomodulatory effect of LAB human peripheral blood mononuclear cells.

Results: All of the metabolites tested showed statistically significant antibiofilm activity on biofilms of pathogenic microorganisms. Although there was a difference between metabolites, Lactobacullus paracasei L2 and L20 strains had a high inhibitory effect on S. aureus (95.1%) and P. aeruginosa by 92.7%, respectively. L. plantarum L8 strain had 95.7% antibiofilm activity on E. coli. It was also determined that LAB has anti-QS activities in different concentrations. The immunomodulatory effect of LAB was found to produce higher IFN‐ γ than the controls, whereas IL-10 concentrations were lower.

Conclusion: Bacteria use QS to regulate various sequences of functions, including virulence and biofilm formation. Therefore, using bacteria with strong probiotic properties as QS inhibitory agents seems to be a promising approach to reduce or suppress biofilm formation of pathogenic bacteria.

Supporting Institution

Kırşehir Ahi Evran University Scientific Research Projects

Project Number

PYO-FEN.4001.16.012.

Thanks

We would like to thank the scientific research projects coordinator of Kirsehir Ahi Evran University for their financial contributions.

References

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  • 25. Shokri D, Khorasgani MR, Mohkam M, Fatemi SM, Ghasemi Y, Ta-heri-Kafrani A. The inhibition effect of lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa. Probiotics An-timicrobe Proteins 2018; 10(1): 34-42. google scholar
  • 26. Lee D, Kim BS, Kang SS. Bacteriocin of Pediococcus acidilactici HW01 inhibits biofilm formation and virulence factor production by Pseu-domonas aeruginosa. Probiotics Antimicro 2020; 12(1): 73-81. google scholar
  • 27. Wasfi R, Abd El-Rahman OA, Zafer MM, Ashour HM. Probiotic Lac-tobacillus sp. inhibit growth, biofilm formation and gene expres-sion of caries-inducing Streptococcus mutans. J Cell Mol Med 2018; 22(3): 1972-83. google scholar
  • 28. James KM, MacDonald KW, Chanyi RM, Cadieux PA, Burton JP. In-hibition of Candida albicans biofilm formation and modulation of gene expression by probiotic cells and supernatant. J Med Micro-biol 2016; 65(4): 328-36. google scholar
  • 29. Kiymaci ME, Altanlar N, Gumustas M, Ozkan SA, Akin A. Quorum sensing signals and related virulence inhibition of Pseudomonas aeruginosa by a potential probiotic strain’s organic acid. Microb Pathog 2018; 121: 190-7. google scholar
  • 30. Gömez NC, Ramiro JM, Quecan BX, de Melo Franco BD. Use of po-tential probiotic lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes, Salmonella typhimurium, and Escherich-ia coli O157: H7 biofilms formation. Front Microbiol 2016; 7: 863. google scholar
  • 31. Qichuang Li, Yonglong Pan, Linxian Ding, Huachang Hong, Shuxia Yan, Binbin Wu, et al. draft genome sequence of Lactobacillus bre-vis Strain 3M004, a probiotic with potential quorum-sensing regu-lation. Genome Announc 2017; 5(36): e00675-17. google scholar
  • 32. Merghni A, Dallel I, Noumi E. Antioxidant and antiproliferative po-tential of biosurfactants isolated from Lactobacillus casei and their antibiofilm effect in oral Staphylococcus aureus strains. Microb Pat-hog 2017; 104: 84-9. google scholar
  • 33. Yan X, Gu S, Cui X. Antimicrobial, anti-adhesive and antibiofilm potential of biosurfactants isolated from Pediococcus acidilac-tici and Lactobacillus plantarum against Staphylococcus aureus CMCC26003. Microb Pathog 2019; 127: 12-20. google scholar
  • 34. Rana S, Bhawal S, Kumari A, Kapila S, Kapila R. pH-dependent inhi-bition of AHL-mediated quorum sensing by cell-free supernatant of lactic acid bacteria in Pseudomonas aeruginosa PAO1. Microb Pathog 2020; 28; 142: 104105. google scholar
  • 35. Sturme MHJ, Francke C, Siezen RJ, de Vos WM, Kleerebezem M. Making sense of quorum sensing in lactobacilli: a special focus on Lactobacillus plantarum WCFS1. Microbiology 2007; 153: 3939-47. google scholar
  • 36. Yılmaz-Yıldıran H, Karahan AG, Başyiğit-Kılıç G. Quorum sensing mechanism in lactic acid bacteria. Turk J Hygiene Exper Bio 2015; 72(1): 79-90. google scholar
  • 37. Tabasco R, de Palencia PF, Fontecha Pelaez C, Requena T. Compe-tition mechanisms of lactic acid bacteria and bifidobacteria: fer-mentative metabolism and colonization. LWT-Food Sci Technol 2014; 55: 680-4. google scholar
  • 38. Aldunate M, Srbinovski D, Hearps AC, Latham CF, Ramsland PA, Gu-gasyan R, Cone RA, Tachedjian G. Antimicrobial and immune mod-ulatory effects of lactic acid and short chain fatty acids produced by vaginal microbiota associated with eubiosis and bacterial vagi-nosis. Front Physiol 2015; 6: 164. google scholar
Year 2021, Volume: 80 Issue: 2, 82 - 90, 17.12.2021
https://doi.org/10.26650/EurJBiol.2021.932640

Abstract

Project Number

PYO-FEN.4001.16.012.

References

  • 1. Abdula N, Macharia J, Motsoaledi A, Swaminathan S, Vijay Ragha-van K. National action for global gains in antimicrobial resistance. Lancet 2016; 387: 3-5. google scholar
  • 2. Fuqua C, Greenberg EP. Self-perception in bacteria: quorum sens-ing with acylated homoserine lactones. Curr Opin Microbiol 1998; 2: 183-9. google scholar
  • 3. Theodora NA, Dominika V, Waturangi DE. Screening and quantifi-cation of anti-quorum sensing and antibiofilm activities of phyllo-sphere bacteria against biofilm forming bacteria. BMC Res Notes 2019; 12(1): 732. google scholar
  • 4. Zhao X, Yu Z, Ding T. Quorum-sensing regulation of antimicrobial resistance in bacteria. Microorganisms 2020; 8(3): 425. google scholar
  • 5. Barzegari A, Kheyrolahzadeh K, Khatibi SMH, Sharifi S, Memar MY, Vahed SZ. The battle of probiotics and their derivatives against biofilms. Infect Drug Resist 2020; 13: 659-72. google scholar
  • 6. KrzyzekP.Challenges and limitations ofanti-quorum sensing therapies. Front Microbiol 2019; 31(10): 2473. google scholar
  • 7. Hawver LA, Jung S A, Ng WL. Specificity and complexity in bacterial quorum-sensing systems. FEMS Microbiol Rev 2016; 40(5): 738-52. google scholar
  • 8. Liu L, Wu R, Zhang J, Shang N, Li P. D-Ribose interferes with quo-rum sensing to inhibit biofilm formation of Lactobacillus paraplan-tarum L-ZS9. Front Microbiol 2017; 8: 1860. google scholar
  • 9. Jiang Q, Chen J, Yang C, Yin Y, Yao K. Quorum Sensing: A prospec-tive therapeutic target for bacterial diseases. Biomed Res Int 2019; 2015978. google scholar
  • 10. Kiray E, Kariptas E, Dagli SS. Evaluation of vaginal lactobacilli with potential probiotic properties and biotherapeutic effects isolated from healthy Turkish women. Fresenius Environ Bull 2020; 29424052. google scholar
  • 11. Sharma V, Harjai K, Shukla G. Effect of bacteriocin and exopoly-saccharides isolated from probiotic on P. aeruginosa PAO1 biofilm. Folia Microbiol 2018; 63: 181-90. google scholar
  • 12. Kiray E, Kariptas E, Azarkan SA. Evaluation of vaginal lactobacilli with potential probiotic properties and biotherapeutic effects iso-lated from healthy Turkish women. PSM Micro 2019; 4(3): 56-70. google scholar
  • 13. Abudoleh SM, Mahasneh AM. Anti-quorum sensing activity of substances isolated from wild berry associated bacteria. Avicenna J Med Biotechnol 2017; 9(1): 23-30. google scholar
  • 14. Younis KM, Usup G, Ahmad A. Secondary metabolites produced by marine streptomyces as antibiofilm and anti-quorum sensing inhibitor of uropathogen Proteus mirabilis. Environ Sci Pollut Res 2015; 23(5): 4756-67. google scholar
  • 15. Tabbouche SA, Gurgen A, Yildiz S, Kilic AO, Sokmen M. Antimicro-bial and antiquorum sensing activity of some wild mushrooms collected from Turkey. MSU J Sci 2017; 5(2): 453-7. google scholar
  • 16. Han MSA, Zahin M, Hasan S, Husain FM, Ahmad I. Inhibition of quo-rum sensing regulated bacterial functionsby plant essential oils with special reference to clove oil. Lett Appl Microbiol 2009; 49(3): 354-60. google scholar
  • 17. Vissers YM, Snel J, Zuurendonk PF, Smit BA, Wichers HJ, Savelkoul HFJ. Differential effects of Lactobacillus acidophilus and Lactobacil-lus plantarum strains on cytokine induction in human peripheral blood mononuclear cells. FEMS Immunol Med Microbiol 2010; 59(1): 60-70. google scholar
  • 18. Forsberg MM, Björkander S, Pang Y, Lundqvist L, Ndi M, Ott M, et al. Extracellular membrane vesicles from lactobacilli dampen IFN-y responses in a monocyte-dependent Manner Sci Rep 2019; 9: 17109. google scholar
  • 19. Jamal M, Ahmad W, Andleeb S, Jalil F, Imran M, Nawaz MA, et al. Bacterial biofilm and associated infections. JCMA 2018; 81(1): 7-11. google scholar
  • 20. Onbas T, Osmanagaoglu O, Kiran F. Potential properties of Lacto-bacillus plantarum F-10 as a bio-control strategy for wound infec-tions. Probiotics Antimicro 2019; 11(4): 1110- 23. google scholar
  • 21. Garcia-Contreras R, Nunez-Lopez L, Jasso-Chavez R. Quorum sens-ing enhancement of the stress response promotes resistance to quorum quenching and prevents social cheating. ISME J 2015; 9(1): 115-25. google scholar
  • 22. Kim NN, Kim WJ, Kang SSJFC. Antibiofilm effect of crude bacterio-cin derived from Lactobacillus brevis DF01 on Escherichia coli and Salmonella typhimurium. Food Control 2019; 98: 274-80. google scholar
  • 23. Kaur S, Sharma P, Kalia N, Singh J, Kaur S. Antibiofilm properties of the fecal probiotic lactobacilli against vibrio spp. Front Cell Infect Microbiol 2018; 8: 120. google scholar
  • 24. Iannitti T, Palmieri B. Therapeutical use of probiotic formulations in clinical practice. Clin Nutr 2010; 29(6): 701-25. google scholar
  • 25. Shokri D, Khorasgani MR, Mohkam M, Fatemi SM, Ghasemi Y, Ta-heri-Kafrani A. The inhibition effect of lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa. Probiotics An-timicrobe Proteins 2018; 10(1): 34-42. google scholar
  • 26. Lee D, Kim BS, Kang SS. Bacteriocin of Pediococcus acidilactici HW01 inhibits biofilm formation and virulence factor production by Pseu-domonas aeruginosa. Probiotics Antimicro 2020; 12(1): 73-81. google scholar
  • 27. Wasfi R, Abd El-Rahman OA, Zafer MM, Ashour HM. Probiotic Lac-tobacillus sp. inhibit growth, biofilm formation and gene expres-sion of caries-inducing Streptococcus mutans. J Cell Mol Med 2018; 22(3): 1972-83. google scholar
  • 28. James KM, MacDonald KW, Chanyi RM, Cadieux PA, Burton JP. In-hibition of Candida albicans biofilm formation and modulation of gene expression by probiotic cells and supernatant. J Med Micro-biol 2016; 65(4): 328-36. google scholar
  • 29. Kiymaci ME, Altanlar N, Gumustas M, Ozkan SA, Akin A. Quorum sensing signals and related virulence inhibition of Pseudomonas aeruginosa by a potential probiotic strain’s organic acid. Microb Pathog 2018; 121: 190-7. google scholar
  • 30. Gömez NC, Ramiro JM, Quecan BX, de Melo Franco BD. Use of po-tential probiotic lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes, Salmonella typhimurium, and Escherich-ia coli O157: H7 biofilms formation. Front Microbiol 2016; 7: 863. google scholar
  • 31. Qichuang Li, Yonglong Pan, Linxian Ding, Huachang Hong, Shuxia Yan, Binbin Wu, et al. draft genome sequence of Lactobacillus bre-vis Strain 3M004, a probiotic with potential quorum-sensing regu-lation. Genome Announc 2017; 5(36): e00675-17. google scholar
  • 32. Merghni A, Dallel I, Noumi E. Antioxidant and antiproliferative po-tential of biosurfactants isolated from Lactobacillus casei and their antibiofilm effect in oral Staphylococcus aureus strains. Microb Pat-hog 2017; 104: 84-9. google scholar
  • 33. Yan X, Gu S, Cui X. Antimicrobial, anti-adhesive and antibiofilm potential of biosurfactants isolated from Pediococcus acidilac-tici and Lactobacillus plantarum against Staphylococcus aureus CMCC26003. Microb Pathog 2019; 127: 12-20. google scholar
  • 34. Rana S, Bhawal S, Kumari A, Kapila S, Kapila R. pH-dependent inhi-bition of AHL-mediated quorum sensing by cell-free supernatant of lactic acid bacteria in Pseudomonas aeruginosa PAO1. Microb Pathog 2020; 28; 142: 104105. google scholar
  • 35. Sturme MHJ, Francke C, Siezen RJ, de Vos WM, Kleerebezem M. Making sense of quorum sensing in lactobacilli: a special focus on Lactobacillus plantarum WCFS1. Microbiology 2007; 153: 3939-47. google scholar
  • 36. Yılmaz-Yıldıran H, Karahan AG, Başyiğit-Kılıç G. Quorum sensing mechanism in lactic acid bacteria. Turk J Hygiene Exper Bio 2015; 72(1): 79-90. google scholar
  • 37. Tabasco R, de Palencia PF, Fontecha Pelaez C, Requena T. Compe-tition mechanisms of lactic acid bacteria and bifidobacteria: fer-mentative metabolism and colonization. LWT-Food Sci Technol 2014; 55: 680-4. google scholar
  • 38. Aldunate M, Srbinovski D, Hearps AC, Latham CF, Ramsland PA, Gu-gasyan R, Cone RA, Tachedjian G. Antimicrobial and immune mod-ulatory effects of lactic acid and short chain fatty acids produced by vaginal microbiota associated with eubiosis and bacterial vagi-nosis. Front Physiol 2015; 6: 164. google scholar
There are 38 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Esin Kıray 0000-0002-6908-5909

Project Number PYO-FEN.4001.16.012.
Publication Date December 17, 2021
Submission Date May 4, 2021
Published in Issue Year 2021 Volume: 80 Issue: 2

Cite

AMA Kıray E. Antibiofilm and Anti-Quorum Sensing Activities of Vaginal Origin Probiotics. Eur J Biol. December 2021;80(2):82-90. doi:10.26650/EurJBiol.2021.932640