Isolation of Aerobic Heterotrophic and Anaerobic Sulphate Reducing Bacteria from Model Water System by Filtration Method

Year 2008, Volume: 67 Issue: 1, 33 - 38, 17.11.2010

Esra Ilhan Sungur , Bihter Minnoş Nihal Dogruoz

Abstract

 

 

The purpose of this study was to compare the efficacy of the filtration method with direct method (without filtration) for detection and enumeration of either aerobic heterotrophic bacteria or anaerobic sulphate reducing bacteria (SRB). In addition we compared the results of colony morphology types of heterotrophic bacteria performed by filtration-spread plate experiments with spread plate experiments (i.e. plating on R2A agar plates). We detected that there was no statistically significant difference in the mean of SRB and heterotrophic bacteria counts from unfiltered and filtered samples. The results of the experiments comparing the cultivation and enumeration success of the filtered samples with unfiltered samples clearly demonstrate that filtration method enables the cultivation and enumeration of SRB and heterotrophic bacteria. In addition, the numbers of distinct colony types recovered from filtered samples were usually higher than unfiltered samples.

Keywords

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References

• APHA (1981) Standards methods for the examination of water and wastewater. American Public Health Association, Washington DC.
• British Standard (1998) Water Quality Part 4: Microbiological Enumeration of Legionella. BS 6068-4.12. ISO 11731. Detection and
• Choudhary S.G. (1998) Emerging microbial control issues in cooling water systems. Hydrocarbon Processing, 77 (5): 91-102.
• Hamilton W.A. (1985) Sulphate reducing bacteria and anaerobic corrosion. Annual Review of Microbiology, 39: 195-217. E. Ilhan-Sungur and Cotuk A. (2005) Characterization of sulfate reducing bacteria isolated from cooling towers. Environmental Monitoring and Assessment, 104: 211–219.
• Ilhan-Sungur E., Cansever N. and Cotuk A. (2007) Microbial corrosion of galvanized steel by a freshwater strain of sulphate reducing bacteria (Desulfovibrio sp.). Corrosion Science, 49: 1097-1109.
• Postgate J.R. (1984) The sulphate reducing bacteria, 2nd Cambridge. University Press,
• Punakabutra N., Nunthapisud P., Pisitkun T., Tiranathanagul K., Tungsanga K. and Eiam- Ong M.D., S. (2004) Comparison of different culture methods on bacterial recovery in hemodialysis fluids. Journal of the Medical Association of Thailand, 87(11), 1361-1367.
• Reasoner D.J. and Geldrich E.E. (1985) A new medium for the enumeration and subculture of bacteria from potable water. Applied and Environmental Microbiology, 49: 1-7.
• The Institute of Petroleum (1995) Determination of the viable microbial content of fuels and fuel components boiling below 390 _C – Filtration and Culture Method. IP Method Number 385/95.
• Türetgen I., Ilhan-Sungur E. and Cotuk A. (2005) Enumeration of Legionella pneumophila in cooling tower water systems. Environmental Monitoring and Assessment, 100 (1-3): 53-58.
• Widdel F. and Pfennig N. (1984) Dissimilatory sulfate reducing bacteria. In: Noel, R.K. (ed.), Systematic bacteriology, Williams & Wilkins, Baltimore, USA, pp. 663-679.