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Toxic effects of water/alcoholic extract of Syzygium aromaticum on sperm quality, sex hormones and reproductive tissues in male mouse

Year 2012, Volume: 71 Issue: 2, 95 - 102, 25.12.2012

Abstract

Syzygium aromaticum was considered as an aphrodisiac agent; however, it may cause some side effects.
The aim of this study was to examine the effects of Syzygium aromaticum extract on male reproductive
system. To do this, forty male mice were divided into 5 groups: negative control was fed with vehicle,
positive control was fed with aphridite and three experimental groups were fed with 250, 500 and 1000
mg/kg/day of water/alcohol extract of S. aromaticum for 34 days. The sperm count and motility and also
the sex hormones level were assessed. The reproductive tissues were prepared histologically and studied
under the light microscope. The results indicated the high dose-treated animals showed a significant
decline in sperm count, motility and testosterone but a significant increase in estradiol concentration
compared with the control group. The seminiferous tubules of extract-treated animals contained fewer
sperms than in those of control animals. It seems that in spite of aphrodiastic activity of the Syzygium
aromaticum extract, it reduced spermatogenesis.
Keywords: Reproduction, Syzygium aromaticum, Sperm

References

  • Abdwl-Wahhab M.A. and Aly S.E. (2005) Antioxi- dant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rat during aflatoxicosis. Journal of Applied Toxicology, 25(3): 218-23.
  • Baberjee S., Panda C.K. and Das S. (2006) Clove (Syzygium aromaticum L.), a potential chemo- preventive agent for lung cancer. Carcinogen- esis, 27(8): 1645-54.
  • Ben Abdallah F., Fetoui H., Zribi N., Fakfakh F. and Ammar-Keskes L . (2012) Antioxidant supplementations in vitro improve rat sperm parameters and enhance antioxidant enzyme activities against dimethoate-induced sperm damages. Andrologia, doi: 10.1111/j.1439- 0272.2011.01177.x.
  • Buch J.G., Dikshit R.K. and Mansuri S.M. (1998) Effect of certain volatile oils on human sper- matozoa. Indian Journal of Medical Research, 87: 361–363.
  • Carrasco F.R., Schmidt G., Romero A.L.,Sartoretto J.L., Bersani-Amado C.A. and Cuman R.K. (2009) Immunomodulatory activity of Zingiber officinale Roscoe, Salvia officinalis L. and Syz- ygium aromaticum L. essential oils: evidence for humor- and cell-mediated responses. Journal of pharmacology & pharmacotherapeutics, 61(7): 961-7.
  • Chaieb K., Hajlaoui H., Zamantar T., Kahla-Na- kbi A.B., Rouabhia M., Mahdouani K. and Bakhrouf F. (2007) The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzugium aromaticum L Myrtaceae): a short review. Phytotherapy Re- search, 21(6): 501–506.
  • Chan K. (2003) Some aspects of toxic contami- nants in herbal medicines. Chemosphere, 52 (9): 1361–1371.
  • Gulcin I. (2011) Antioxidant activity of eugenol: a structure-activity relationship study. Journal of Medicinal Food, 14 (9): 975-85.
  • Lucki N.C. and Sewer M.B. (2011) Genistein stimulates mcf-7 breast cancer cell growth by inducing acid ceramidase (asah1) gene expression. Journal of Biological Chemistry, 286(22):19399-409.
  • Micinski P., Pawlicki K., .Wielgus E., Bochenek M., Gogol P. and Ficek B. (2011) Total reactive antioxidant potential and DNA fragmentation index as fertility sperm parameters. Reproduc- tive Biology, 11 (2): 135-44.
  • Miksicek R.J. (1995) Estrogenic flavonoids: struc- tural requirements for biological activity. Pro- ceedings of the Society for Experimental Biology and Medicine, 208 (1): 44-50.
  • Mishra R.K. and Singh S.K. (2008) Safety as- sessment of Syzygium aromaticum flower bud (clove) extract with respect to testicular func- tion in mice. Food and Chemical Toxicology, 46 (10): 3333–3338.
  • Miyazawa M. and Hisama M. (2003) Antimutagenic activity of Phenylpropanoids from clove (Syzy- gium aromaticum) Journal of agricultural and food chemistry, 51 (22):6413–6422.
  • Nassar M.I. (2006) Flavonoid triglycosides from the seeds of Syzygium aromaticum. Carbohydrate Research, 341(1): 160-3.
  • Pinto E., Vale-Silva L., Cavaleiro C. and Salgueiro L. (2009) Antifungal activity of the clove essen- tial oil from Syzygium aromaticum on Candida, Aspergillus and Dermatophyte species. Journal of Medical Microbiology, 58 (11):1454-62.
  • Rastogi R.P. and Mehrotra B.N. (1984) Syzygium aromaticum. In: Rastogi R.P.and Mehrotra B.N., (edi), Compendium of Indian Medicinal Plants. III. Central Drug Research Institute, Lucknow, India, pp. 620.
  • Sweetman S. (2009) Martindale Pharmacology. 37th Edition, World color book service, Mas- sachusett.
  • Tajuddin, Ahmad S., Latif A. and Qasmi I.A. (2003) Aphrodisiac activity of 50% ethanolic extracts of Myristica fragrans Houtt. (nutmeg) and Syzygium aromaticum (L) Merr. & Perry. (clove) in male mice: a comparative study. BMC Complementary and Alternative Medicine, 46: 3333-3336.
  • Vanitakumari G., Sampatharaj R., Selvaraj M. and Thangamani R. (1998) Effect of short-term treat- ment of eugenol on seminal vesicle of adult albino rats. Indian Journal of Experimental Biology, 36 (12): 1240–1244.
  • World Health Organization: (1999) Laboratory manual for the examination of human semen and sperm-cervical mucus interaction (4th ed.), Cambridge University Press, Cambridge.
  • Yakubu M.T. and Akanji M.A. (2011) Effect of Aqueous Extract of Massularia acuminata Stem on Sexual Behaviour of Male Wistar Rats. Ev- id Based Complement Alternative Medicine, 738103. doi: 10,1155/2011/738103
  • Zheng G.Q. and Kenney P.M. (1992) Sesqueter- penes from clove (Eugenia caryophyllata) as potential anti carcinogenic agents. Journal of Natural Product, 55 (7): 999–1003.
Year 2012, Volume: 71 Issue: 2, 95 - 102, 25.12.2012

Abstract

References

  • Abdwl-Wahhab M.A. and Aly S.E. (2005) Antioxi- dant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rat during aflatoxicosis. Journal of Applied Toxicology, 25(3): 218-23.
  • Baberjee S., Panda C.K. and Das S. (2006) Clove (Syzygium aromaticum L.), a potential chemo- preventive agent for lung cancer. Carcinogen- esis, 27(8): 1645-54.
  • Ben Abdallah F., Fetoui H., Zribi N., Fakfakh F. and Ammar-Keskes L . (2012) Antioxidant supplementations in vitro improve rat sperm parameters and enhance antioxidant enzyme activities against dimethoate-induced sperm damages. Andrologia, doi: 10.1111/j.1439- 0272.2011.01177.x.
  • Buch J.G., Dikshit R.K. and Mansuri S.M. (1998) Effect of certain volatile oils on human sper- matozoa. Indian Journal of Medical Research, 87: 361–363.
  • Carrasco F.R., Schmidt G., Romero A.L.,Sartoretto J.L., Bersani-Amado C.A. and Cuman R.K. (2009) Immunomodulatory activity of Zingiber officinale Roscoe, Salvia officinalis L. and Syz- ygium aromaticum L. essential oils: evidence for humor- and cell-mediated responses. Journal of pharmacology & pharmacotherapeutics, 61(7): 961-7.
  • Chaieb K., Hajlaoui H., Zamantar T., Kahla-Na- kbi A.B., Rouabhia M., Mahdouani K. and Bakhrouf F. (2007) The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzugium aromaticum L Myrtaceae): a short review. Phytotherapy Re- search, 21(6): 501–506.
  • Chan K. (2003) Some aspects of toxic contami- nants in herbal medicines. Chemosphere, 52 (9): 1361–1371.
  • Gulcin I. (2011) Antioxidant activity of eugenol: a structure-activity relationship study. Journal of Medicinal Food, 14 (9): 975-85.
  • Lucki N.C. and Sewer M.B. (2011) Genistein stimulates mcf-7 breast cancer cell growth by inducing acid ceramidase (asah1) gene expression. Journal of Biological Chemistry, 286(22):19399-409.
  • Micinski P., Pawlicki K., .Wielgus E., Bochenek M., Gogol P. and Ficek B. (2011) Total reactive antioxidant potential and DNA fragmentation index as fertility sperm parameters. Reproduc- tive Biology, 11 (2): 135-44.
  • Miksicek R.J. (1995) Estrogenic flavonoids: struc- tural requirements for biological activity. Pro- ceedings of the Society for Experimental Biology and Medicine, 208 (1): 44-50.
  • Mishra R.K. and Singh S.K. (2008) Safety as- sessment of Syzygium aromaticum flower bud (clove) extract with respect to testicular func- tion in mice. Food and Chemical Toxicology, 46 (10): 3333–3338.
  • Miyazawa M. and Hisama M. (2003) Antimutagenic activity of Phenylpropanoids from clove (Syzy- gium aromaticum) Journal of agricultural and food chemistry, 51 (22):6413–6422.
  • Nassar M.I. (2006) Flavonoid triglycosides from the seeds of Syzygium aromaticum. Carbohydrate Research, 341(1): 160-3.
  • Pinto E., Vale-Silva L., Cavaleiro C. and Salgueiro L. (2009) Antifungal activity of the clove essen- tial oil from Syzygium aromaticum on Candida, Aspergillus and Dermatophyte species. Journal of Medical Microbiology, 58 (11):1454-62.
  • Rastogi R.P. and Mehrotra B.N. (1984) Syzygium aromaticum. In: Rastogi R.P.and Mehrotra B.N., (edi), Compendium of Indian Medicinal Plants. III. Central Drug Research Institute, Lucknow, India, pp. 620.
  • Sweetman S. (2009) Martindale Pharmacology. 37th Edition, World color book service, Mas- sachusett.
  • Tajuddin, Ahmad S., Latif A. and Qasmi I.A. (2003) Aphrodisiac activity of 50% ethanolic extracts of Myristica fragrans Houtt. (nutmeg) and Syzygium aromaticum (L) Merr. & Perry. (clove) in male mice: a comparative study. BMC Complementary and Alternative Medicine, 46: 3333-3336.
  • Vanitakumari G., Sampatharaj R., Selvaraj M. and Thangamani R. (1998) Effect of short-term treat- ment of eugenol on seminal vesicle of adult albino rats. Indian Journal of Experimental Biology, 36 (12): 1240–1244.
  • World Health Organization: (1999) Laboratory manual for the examination of human semen and sperm-cervical mucus interaction (4th ed.), Cambridge University Press, Cambridge.
  • Yakubu M.T. and Akanji M.A. (2011) Effect of Aqueous Extract of Massularia acuminata Stem on Sexual Behaviour of Male Wistar Rats. Ev- id Based Complement Alternative Medicine, 738103. doi: 10,1155/2011/738103
  • Zheng G.Q. and Kenney P.M. (1992) Sesqueter- penes from clove (Eugenia caryophyllata) as potential anti carcinogenic agents. Journal of Natural Product, 55 (7): 999–1003.
There are 22 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Farzaneh Dehghani This is me

Aseyeh Heshmatpour This is me

Mohammad Panjehshahin This is me

Tahereh Talaei-khozani This is me

Publication Date December 25, 2012
Submission Date December 25, 2012
Published in Issue Year 2012 Volume: 71 Issue: 2

Cite

AMA Dehghani F, Heshmatpour A, Panjehshahin M, Talaei-khozani T. Toxic effects of water/alcoholic extract of Syzygium aromaticum on sperm quality, sex hormones and reproductive tissues in male mouse. Eur J Biol. November 2012;71(2):95-102.