Antibacterial and hemolytic activities of different extracts of Amsonia orientalis Decne (Apocynaceae)

Year 2013, Volume: 72 Issue: 1, 1 - 8, 06.11.2013

A. Kimiran Erdem E. Ö. Arslan Aydoğdu G. Cevahir Öz Ç. Erol M. Yazgan

Abstract

In the current study, the antibacterial activities of the crude extracts (leaf and stem chloroform and methanol; leaf hexane, acetone, deionized water and ethanol; stem deionized water) of Amsonia orientalis Decne. (Blue Star) were investigated against 17 different strains of Gram-negative and Gram-positive bacteria. To evaluate the antibacterial and hemolitic activities of extracts standard antibiotic discs and human erythrocytes were used, respectively. The antibacterial activity was determed in the all extracts, except the hexane extracts of the leaves. It was observed that Bacillus subtilis ATCC 6633, Legionella pneumophila ATCC 33152 and L. pneumophila serogroup (SG) 2-14 are the most susceptible bacteria to the different extracts, while Pseudomonas aeruginosa ATCC 9327, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 10541 and Proteus mirabilis are resistant bacteria. It was determined that chloroform (leaf and stem), hexane (leaf) and deionized water (stem) extracts did not show hemolytic activity, while ethanol (leaf) (≥5 mg/ml), methanol (stem and leaf) (≥10 mg/ml), acetone (leaf) (≥10 mg/ml) and deionized water (leaf) (≥20 mg/ml) extracts showed hemolytic activity.
Key words: Amsonia orientalis, Antibacterial activity, Hemolytic activity, Plant extract.
*Corresponding Author: Elif Özlem ARSLAN AYDOĞDU (e-mail: [e-mail])
(Received: 01.03.2012 Accepted: 21.09.2012)

Keywords

Amsonia orientalis, Antibacterial activity, Hemolytic activity, Plant extract.

Farklı Amsonia orientalis Decne (Apocynaceae) ekstraktlarının antibakteriyel ve hemolitik aktiviteleri

Abstract

Çalışmamızda Amsonia orientalis Decne. (Mavi Yıldız)’ nin ham ekstraktlarının (gövde ve yaprağın kloroform ve metanol; yaprağın hekzan, aseton, deiyonize su ve etanol; gövde dokusunun deiyonize su ekstraktları) 17 farklı Gram-negatif ve Gram-pozitif bakteriye karşı antibakteriyel aktiviteleri incelenmiştir. Ekstraktların antibakteriyel ve hemolitik etkileri sırasıyla standart antibiyotik diskler ve insan eritrositleri kullanılarak değerlendirilmiştir. Yaprakların hekzan ekstraktı ile yaprak+gövde dokusunun kloroform ekstraktı dışındaki tüm ekstraktlarda antibakteriyel aktivite saptanmıştır. Pseudomonas aeruginosa ATCC 9327, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 10541 ve Proteus mirabilis’ in denenen ekstraktlara karşı üreme inhibisyon zonu oluşturmadığı; Bacillus subtilis ATCC 6633, Legionella pneumophila ATCC 33152 ve L. pneumophila SG 2-14’ ün ise inhibisyon zonu oluşturduğu saptanmıştır. Yaprağın etanol (≥5 mg/ml), aseton (≥10 mg/ml) ve deiyonize su (≥20 mg/ml) ekstraktları
ile yaprak+gövde dokusunun metanol (≥10 mg/ml) ekstraktı hemolitik aktivite gösterirken, yaprak+gövde
dokusunun kloroform, yaprağın hekzan ve gövde dokusunun deiyonize su ekstraktlarının hemolitik
aktivite göstermediği saptanmıştır.

Keywords

Amsonia orientalis, Antibakteriyel aktivite, Hemolitik aktivite, Bitki ekstraktı

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