Determination of Optimum Immobilization Conditions of Trametes versicolor Laccase with Sodium Alginate Beads

Year 2013, Volume: 72 Issue: 2, 15 - 21, 12.05.2014

Arzu Ünal , Nazif Kolankaya

Abstract

 

Laccase (EC 1.10.3.2) is a multi copper enzyme that catalyzes the oxidation of various environmental

pollutants such as phenolic compounds. The efficiency of the enzyme for environmental and industrial

applications can be increased by immobilizing the enzyme on a carrier. In the present study, laccase

obtained from Trametes versicolor (fungi) was immobilized on sodium alginate beads and kappacarrageenan

and the effect of contact time, pH, temperature, the amount of carrier and enzyme

concentration were investigated to determine optimum conditions of laccase immobilization. Sodium

alginate beads were chosen as the most efficient carrier due to their high immobilization yield. Maximum

laccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200

mg of sodium alginate amount.

 

Keywords

Laccase, sodium alginate, kappa-carrageenan, immobilization.Corresponding author: Arzu Ünal (e-mail: [e-mail])

Aljinat Boncukları ile Trametes versicolor Lakkazının Optimum İmmobilizasyon Koşullarının Belirlenmesi

Abstract

Lakkaz (EC 1.10.3.2), fenolik bileşikler gibi çeşitli çevresel kirleticilerin oksidasyonunu katalizleyen çoklu bakır enzimidir. Çevresel ve endüstriyel uygulamalar için bir taşıyıcı üzerinde enzimin immobilize edilmesiyle etkinliği arttırılabilir. Bu çalışmada, Trametes versicolor’dan elde edilen lakkaz sodium aljinat ve kappa-karagen üzerinde immobilize edilmiş, temas süresi, pH, sıcaklık, taşıyıcı miktarı, enzim konsantrasyonun etkisi değerlendirilmiştir. Sodyum aljinat ve kappa-karragenanın immobilizasyon etkinliği araştırılmıştır. Sonuç olarak, sodyum aljinat boncukları yüksek immobilizasyon veriminden dolayı en iyi taşıyıcı olarak seçilmiştir. Maksimum lakkaz immobilizasyonu, 30 dakika temas süresi, pH 4.5, 30 °C, 200 mg sodyum aljinat koşullarında elde edilmiştir.

Keywords

Lakkaz, sodyum aljinat, kappa-karragenan, immobilizasyon

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