POLİPOİD ENDOMETRİOZİS PATOGENEZİNDE BETA-KATENİN VE FOXP1’İN ROLÜ

Abstract

Amaç: Polipoid endometriozis (PE) patogenezinde Forkhead box protein P1 (FOXP1) ve beta-katenin’nin rolünün araştırılması. Gereç ve Yöntem: Çalışmaya 15 PE olgusu dahil edilmiştir. Klinik bilgiler hastaların tıbbi kayıtlarından ve patoloji raporlarından elde edilmiştir. Tüm mikroskopik preperatlar tanının doğrulanması ve ek mikroskopik özelliklerin tanımlanması amacıyla tekrar değerlendirilmiştir. FOXP1 ve beta-katenin antikorları kullanılarak 15 PE ve kontrol grubu olarak dokuz endometrial polip (EP) ve dokuz ovarian endometriozis (OE) olgusuna immünhistokimyasal inceleme yapılmıştır. Bulgular: Stromal nükleer beta-katenin boyanması altı PE, beş EP ve bir OE olgusunda gözlenmiştir. Stromal FOXP1 boyanması OE olgularına göre PE ve EP olgularında belirgin şekilde azalmış olup tüm OE olgularında stromal FOXP1 boyanması izlenirken beş PE ve iki EP olgusunda stromal FOXP1 boyanması saptanmıştır. PE ile OE ve EP ile OE olguları arasındaki stromal FOXP1 boyanması farkı anlamlıdır (sırasıyla p=0,002 ve p=0,023). Beta-katenin ile PE ve kontrol grubu olguları arasında anlamlı fark bulunmamıştır (p=0,69). Histolojik özelliklerle bu antikorların pozitifliği arasında ilişki yoktur. Sonuç: PE olgularındaki FOXP1 kaybı PE ve konvansiyonel endometriosis arasındaki bir diğer biyolojik fark olarak tanımlanabilir. Ayrıca PE ve EP olgularındaki stromal FOXP1 boyanmasındaki benzerlik FOXP1’in polip oluşumunda rolü olduğunu düşündürmektedir.

Keywords

• Endometriozis
• polip
• immünhistokimya
• FOXP1
• beta-katenin

THE ROLE OF BETA-CATENIN AND FOXP1 IN THE PATHOGENESIS OF POLYPOID ENDOMETRIOSIS

Abstract

Objective: To investigate whether beta catenin and Forkhead box protein P1 (FOXP1) play a role in pathogenesis of polypoid endometriosis (PE). Material and Method: Our study included fifteen cases of PE. Clinical findings were gathered from archived files of relevant clinics and pathology reports. All glass slides were re-examined for confirmation of the diagnosis and the detection of additional microscopic findings. An immunohistochemical examination was performed using anti beta catenin and FOXP1 antibodies in fifteen cases of PE, and in a control group that contained nine cases of endometrial polyps (EP) and nine cases of conventional ovarian endometriosis (OE). Result: Stromal nuclear beta catenin expression was observed in six cases in PE, five cases in EP and one case in the OE group. Stromal FOXP1 staining in PE and EP was significantly reduced as compared to OE. Five PE and two EP cases showed stromal FOXP1 staining while all the OE cases showed stromal FOXP1 staining. The Stromal FOXP1 staining was statistically significant between PE vs OE (p=0.002) and EP vs OE (p=0.023) cases. There was no difference between PE and the control cases in terms of nuclear beta catenin staining (p=0.69). There was no correlation between these two antibodies and histologic features. Conclusion: The loss of stromal FOXP1 is another biological difference of PE and the overall similarity of expression of FOXP1between PE and EP could be regarded as a contributing factor for polyp formation.

Keywords

• Endometriosis
• polyp
• immunohistochemistry
• FOXP1
• beta-catenin

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