STRES KOŞULLARINDA SERUM VE KARACİĞER DOKUSUNDAKİ KATALAZ, SÜPEROKSİT DİSMUTAZ, GLUTATYON PEROKSİDAZ VE MALONDİALDEHİT DÜZEYLERİNİN DEĞİŞİMİ

Year 2024, , 145 - 152, 27.03.2024

Mehmet Deniz Yener , Tuncay Çolak , Özgür Doğa Özsoy , Fatma Ceyla Eraldemir

https://doi.org/10.26650/IUITFD.1387837

Abstract

Amaç: Kronik stres, organ/dokuları etkileyen ve homeostazisi bozan bir faktördür. Bu durum, hücresel bileşenlere zarar veren oksidatif stresin artmasına neden olabilir. Antioksidanlar ise serbest radikalleri notralize ederek bu zararı önlemeye çalışır. Çalışmamızın amacı, kronik hafif stresin serum ve karaciğer dokusundaki Katalaz (CAT), Süperoksit Dismutaz (SOD), Glutatyon Peroksidaz (GPx) ve Malondialdehit (MDA) seviyeleri üzerindeki etkisini araştırmaktır.
Gereç ve Yöntem: Çalışmamızda 16 Wistar albino sıçan kontrol ve deney gruplarına ayrılmıştır. Kronik öngörülemeyen hafif stres model protokolü uygulandı. Serum/karaciğer CAT, SOD, GPx ve MDA düzeyleri Enzyme Linked Immunosorbent Assay metodu ile ölçüldü.
Bulgular: CAT, SOD, GPx ve MDA serum düzeyleri karşılaştırıldığında kontrol ve stres grupları arasında istatistiksel olarak anlamlı fark belirlenmedi (p>0,05). Buna karşın, karaciğer dokularındaki CAT, SOD, GPx ve MDA seviyeleri karşılaştırıldığında, stres grubunda antioksidan enzim seviyelerinde anlamlı yükseliş belirlenmiştir (p<0,05).
Sonuç: Kronik stres altında, antioksidan enzim seviyelerinin karaciğer dokusunda arttığı görülmektedir. Çalışmamızın stres, serbest radikaller ve antioksidanlar arasındaki bağlantıya yönelik bir katkı sunabileceği kanısındayız.

Keywords

Antioksidan, kronik stres, oksidatif stres

ALTERATIONS IN CATALASE, SUPEROXIDE DISMUTASE, GLUTATHIONE PEROXIDASE AND MALONDIALDEHYDE LEVELS IN SERUM AND LIVER TISSUE UNDER STRESS CONDITIONS

Abstract

Objective: Chronic stress is a factor that affects organs/tissues and disrupts homeostasis. This condition can lead to increased oxidative stress, which damages cellular components. Antioxidants attempt to prevent this damage by neutralizing free radicals. Our study aimed to investigate the effect of chronic mild stress on the levels of Catalase (CAT), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx) and Malondialdehyde (MDA) in serum and liver tissue.
Material and Method: In our study, 16 Wistar albino rats were divided into control and experimental groups. The chronic unpredictable mild stress (CUMS) model protocol was employed. The levels of CAT, SOD, GPx, and MDA in serum and liver tissue were measured using the Enzyme-Linked Immunosorbent Assay method.
Result: Upon comparison of serum CAT, SOD, GPx, and MDA levels, no statistically significant difference was observed between the control and stress groups (p>0.05). However, when comparing CAT, SOD, GPx, and MDA levels in the liver tissue, a significant increase in the levels of antioxidant enzymes was noted in the stress group (p<0.05).
Conclusion: Under chronic stress, liver tissue's antioxidant levels appear to increase. We believe our study may contribute to understanding the connection between stress, free radicals, and antioxidants.

Keywords

Antioxidant, chronic stress, oxidative stress

Ethical Statement

Ethical approval of the study was obtained from Animal Experiments Local Ethics Committee, Kocaeli University (6/4-2023).

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