KARMOFUR YÜKLÜ GÜMÜŞ NANOPARTİKÜLLERİNİN SENTEZİ, KARAKTERİZASYONU VE ANTİKANSER POTANSİYELİNİN ARAŞTIRILMASI

Abstract

Amaç: "Üçlü negatif meme kanseri" (TNBC) terimi, östrojen reseptörü (ER), progesteron reseptörü (PR) veya insan epidermal büyüme faktörü reseptörü 2'yi (HER2) eksprese etmeyen tümörleri tanımlamak için kullanılır. TNBC, diğer meme kanseri türlerinden daha agresif olma eğilimindedir. Malign meme kanserinde güncel antineoplastik ilaçların tedavi indeksinin kısıtlayıcı olması, toksisitesi, direnci ve seçici olmaması nedeniyle sınırlı tedavi seçenekleri bulunmaktadır. Bu nedenle, TNBC için yeni tıbbi ilaçların derhal geliştirilmesine ihtiyaç vardır. Burada, MDA-MB-231 ve 4T1 olmak üzere iki TNBC hücre hattı üzerinde karmofur bağlı gümüş nanopartiküllerin (AgNP-Car) büyüme inhibisyonuna etkisini araştırdık ve etkinliklerini kanserli olmayan insan göbek damarı endotel hücreleri (HUVEC) ile karşılaştırdık. Gereç ve Yöntem: AgNP-Car, FTIR, DLS, SEM ve EDX ile sentezlendi ve karakterize edildi. Anti-kanser etkisi 3-(4,5-Dimetiltiyazol- 2-il)-2,5-Difeniltetrazolium Bromür (MTT) testi ile değerlendirildi. Bulgular: AgNP-Car'ın nanopartiküllerin tek başına karmofurdan ağırlıklı olarak daha etkili olduğu belirlendi. Özellikle AgNP-Car uygulanmış 4T1 hücrelerinin, karmofur uygulanmış gruplara göre 5,7 kat daha duyarlı olduğu belirlendi. AgNP'ler HUVEC'ler üzerinde önemli bir toksisite göstermezken; MDA-MB-231 ve 4T1 hücrelerinin sitotoksisitesini önemli ölçüde indüklediler. Sonuç: Sonuçlarımız, AgNP-Car'ın TNBC hücreleri için oldukça güçlü ve seçici büyüme inhibitörü etkisi nedeniyle umut verici bir anti-kanser ajanı olabileceğini göstermektedir.

Keywords

• Gümüş nanopartiküller
• antiproliferatif
• antikanser
• karmofur
• üçlü negatif meme kanseri hücreleri

SYNTHESIS, CHARACTERISATION, AND INVESTIGATION OF THE ANTICANCER POTENTIAL OF CARMOFUR-LOADED SILVER NANOPARTICLES

Abstract

Objective: The term "triple-negative breast cancer" (TNBC) is used to describe tumours that do not express oestrogen receptor (ER), progesterone receptor (PR), or human epidermal growth factor receptor 2 (HER2). TNBC tends to be more aggressive than other types of breast cancer. Current antineoplastic drugs have limited treatment options for malignant breast cancer owing to their narrow therapeutic index, toxicity, resistance, and nonselectivity. Therefore, there is a need for the prompt development of new medicinal drugs for TNBC. Here, we investigated the growth inhibition potential of carmofur-bonded silver nanoparticles (AgNPs-Car) on two TNBC cell lines, MDA-MB-231 and 4T1, and compared the effects with non-cancerous Human umbilical vein endothelial cells (HUVECs). Material and Methods: AgNPs-Car were synthesised and characterisedby FTIR, DLS, SEM, and EDX. The anticancer effect was evaluated using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Results: AgNPs-Car was determined to be predominantly more effective than Car alone. Mainly, 4T1 cells were 5.7-fold more sensitive to AgNPs-Car than Car alone. While AgNPs showed no considerable toxicity on HUVECs, they significantly induced the cytotoxicity of MDA-MB-231 and 4T1 cells. Conclusion: Our results showed that AgNPs-Car is a promising anticancer agent due to its highly potent and selective growth inhibitory effect on TNBC cells.

Keywords

• Silver nanoparticles
• antiproliferative
• anticancer
• Carmofur
• triple-negative breast cancer cells

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