Titanyum Dioksit Nanoparçacıklarının Yac-1 Lenfoma Hücre Soyu Canlılığı Ve İnce Yapısı Üzerine Etkileri

Year 2018, , 17 - 24, 12.03.2018

Tuğba Kotil , Aslı Erdoğan Hasan Serdar Mutlu Sibel Doğan Seyhun Solakoğlu

https://doi.org/10.18017/iuitfd.373670

Abstract

Amaç: Yaygın kullanım alanı olan nanoparçacıkların
kanser terapisinde etkili oldukları bilinmektedir. Çalışmamızda geniş kullanım
alanına sahip titanyum dioksit nanoparçacıklarının (TiO2-NP) YAC-1 lenfoma
hücre soyu üzerine etkilerini incelemeyi amaçladık.

Gereç ve Yöntem: 24’lü kültür kuyucuklarına
ekilen YAC-1 lenfoma hücreleri üzerine iki (0,1mg/mL, 0,5mg/mL) farklı
konsantrasyonda TiO2-NP’si uygulandı. 24 ve 48 saat sonra hücreler toplanarak
canlılık testi için sayma kamarasında sayım yapıldı ve nicel farklılık
Tukey-Kramer çoklu karşılaştırma testi ile belirlendi. Elektron mikroskop incelemesi
için rutin takip sonrası Epon-812 ortamına gömülen hücrelerden alınan ince
kesitler geçirimli elektron mikroskobu ile incelendi.

Bulgular: YAC-1 hücrelerinin çoğalma
indekslerinde TiO2-NP’lerin dozuna ve uygulama süresine bağlı olarak istatistiksel
olarak anlamlı düzeyde azalma izlendi. Elektron mikroskobik incelemede, 24.
saatte 0,5mg uygulanmış grupta ve 48. saatte 0,1 ve 0,5 mg uygulanmış grupta
mitokondri bozulması, otofajik vakuol birikimi ve hem nekrotik hem de apoptotik
hücre morfolojilerine rastlandı.

Sonuç: TiO2-NP’lerinin farklı kanser hücre
soyları üzerinde hücre ölümünü tetiklediği çeşitli çalışmalarla gösterilmiştir.
Çalışmamızda hücre çoğalma indeksi açısından doza ve süreye bağlı azalmaya ek
olarak elektron mikroskobik olarak nanoparçacığa maruz kalan hücrelerde TiO2-NP
içeren otofajik vakuol oluşumları, organellerin yapısında bozulmalar ve
apoptotik ve nekrotik hücre ölümünü gösteren morfolojik değişimleri izledik.
Çevremizde sürekli etkileşim halinde olduğumuz bu TiO2-NP’sinin hücre üzerinde
gösterdiği bu sitotoksik etkinin mekanizmaları, kapsamlı araştırmaların konusu
olarak değerlendirilmelidir.

Keywords

Nanoparçacık, titanyum dioksit, YAC-1, otofaji, apoptoz

The Effects of Titanium Dioxide Nanoparticles on Vitality and Ultrastructure of Yac-1 Lymphoma Cell Line

Abstract

Objective: Nanoparticles, which are widely
used, are known to be effective in cancer therapy. The aim of this study is to
investigate the effects of titanium dioxide nanoparticles (TiO2-NP) on the
YAC-1 lymphoma cell line.

Materials and Methods: TiO2-NP were applied
at two different concentrations (0.1mg/mL, 0.5mg/ mL) on cultured YAC-1
lymphoma cells in 24 well plates. After 24 and 48 hours of treatment the cells
were collected and counted for viability test and the quantitative difference
was determined by the Tukey-Kramer multiple comparison test. Cells were
embedded in Epon-812 after routine process for the electron microscope
examination. Thin sections of the cells were examined with Jem Jeol
transmission electron microscope.

Results: The proliferation index of YAC-1
cells showed a statistically significant decrease with the dose and time
dependent of TiO2-NP treatment. Mitochondrial degeneration, autophagic vacuole
accumulation and both necrotic and apoptotic cell morphologies were observed in
both TiO2-NP treatment groups via electron microscopic evaluation.

Conclusion: Several studies have shown that
TiO2-NPs trigger cell death on different cancer cell lines. In our study, we
observed autophagic vacuole filled with NPs, organelle degeneration, and
morphological changes showing apoptotic and necrotic cell death in groups
exposed to nanoparticles. In addition significant decrease of proliferation
index were detected with dose- and time-dependent. We are constantly
interacting with TiO2-NP in our daily day life. The mechanisms of these
cytotoxic effects of TiO2-NP should be elucidated by further studies.

Keywords

Nanoparticles, titanium dioxide, YAC-1, autophagy, apoptosis

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