SIÇANLARDA TRANEKSAMİK ASİDİN KOAGÜLASYON VE FLEP SAĞKALIMI ÜZERİNE ETKİLERİ

Abstract

Amaç: Traneksamik asit (TNA), perioperatif kan kaybını azaltmak için kullanılan bir antifibrinolitiktir. Çalışmamızda sıçan üzerinde kaldırılan random (rastgele) fleplerde TNA’nın flep nekroz oranı üzerine etkileri incelendi. Bu yolla TNA’nın flep cerrahisi açısından güvenilirliğini sınamak amaçlandı. Gereç ve Yöntem: Çalışmada 14 adet Sprague-Dawley cinsi erkek sıçan kullanıldı. Sıçanlar her grupta yedi sıçan olacak şekilde rastgele iki gruba ayrıldı. Deneyin ilk aşamasında standardize bir “kuyruk kanama modeli” kullanılarak kanamanın durmasına kadar geçen zamanı ölçüldü. Ardından deney grubuna 100 mg/ kg dozunda TNA, kontrol grubuna ise eşit hacimde %0,9 NaCl enjekte edildi ve kanama zamanı ve kanama miktarı ölçümleri tekrarlandı. Sıçanlar operasyon sonrası yedinci günde sakrifiye edilerek flepteki nekroz alanı ölçüldü. Bulgular: TNA verilen sıçanlarda kanama zamanı, ilaç verilmesi öncesinde yapılan kanama zamanı ölçümüne göre istatistiksel olarak anlamlı ölçüde kısa bulundu. Kontrol grubunda ise bu şekilde bir fark izlenmedi. İki grup arasında flep nekroz oranı açısından anlamlı bir fark bulunmadı. Sonuç: Yaptığımız deneysel çalışmada TNA’nın flep sağkalımı üzerine olumsuz bir etkisinin olmadığı görülmüştür.

Keywords

• Pediküllü flep,Traneksamik asit

EFFECTS OF TRANEXAMIC ACID ON COAGULATION AND FLAP SURVIVAL IN RATS

Abstract

Objective: Tranexamic acid (TNA) is an antifibrinolytic agent which is used to decrease blood loss during surgery. In our study, we investigated the effects of TNA on the extent of flap necrosis using random flaps in rats. Our aim was to test the safety of TNA within flap surgery. Material and Method: Fourteen male Sprague-Dawley rats were used. The rats were randomly divided into two groups with 7 rats in each group. For the first stage of the experiment, a “rat tail bleeding model” was used to measure time taken to stop bleeding. The measurement of bleeding time was repeated after an injection of 100 mg/kg TNA in the study group and 0.9% NaCl in the control group in equal volumes. The rats were sacrificed on the seventh postoperative day and the area of necrosis was measured. Results: The bleeding time became shorter in rats after the administration of TNA, while no such effect was observed in the control group. There was no difference between the two groups regarding the flap necrosis ratio. Conclusion: Our study shows that TNA administration does not have a negative effect on random flap survival in rats.

Keywords

• Pedicled flap,Tranexamic Acid,Surgical blood loss

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