KRONİK HEPATİT B HASTALARINDA SERUM IL-17 DÜZEYLERİNİN DEĞERLENDİRİLMESİ

Year 2021, , 68 - 75, 15.01.2021

Bülent Çakal Bilger Çavuş Alp Atasoy Aslı Örmeci Mesut Bulakçı Mine Güllüoğlu Mehmet Güven Günver Filiz Akyüz

https://doi.org/10.26650/IUITFD.2020.0094

Abstract

Amaç: Kronik Hepatit B virüs (HBV) enfeksiyonunun doğal seyri ve klinik sonuçları enfekte bireyin immün yanıtları ve viral faktörler ile yakından ilişkilidir. Serum IL-17 düzeyleri ile HBV ilişkili özellikle şiddetli karaciğer hasarları arasında güçlü bir korelasyon olduğu öne sürülmektedir. Buna karşın IL-17’nin kronik hepatit B hastalığın doğal seyri üzerindeki rolü ise henüz net olarak tanımlanmamıştır. Bu çalışmada kronik hepatit B hastalarının (KHB) histolojik, virolojik, serolojik ve biyokimyasal verileri ile serum IL- 17 düzeyleri arasındaki ilişkinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Kronik HBV enfeksiyonlu 88 hastadan eş zamanlı karaciğer biyopsi ve kan örnekleri elde edildi. Histolojik değerlendirme için Ishak modifiye histolojik aktivite indeksi sistemi kullanıldı. Hastalar fibrozis (F) evrelerine göre yok/hafif (F0- 1), orta/şiddetli (F2-4) ve siroz (F5-6) şeklinde skorlanarak üçer gruba ayrıldı. Serum IL-17 düzeyleri enzim bağlı immunosorbent assay (ELISA) yöntemi kullanılarak analiz edildi. Bulgular: Yaş ortalaması 41,77±13,39, 43’ü erkek 45’i kadın toplam 88 KHB hastası değerlendirildi. Çalışmaya dahil edilen hastaların 64’nün (%72,7) fibrozis evresi F2-4 olarak belirlendi. Serum IL-17 düzeyleri ile hastaların histolojik, virolojik, serolojik ve biyokimyasal verileri arasında istatistiksel olarak anlamlı bir korelasyon saptanmadı. Sonuç: Bu veriler IL-17’nin HBV ilişkili özellikle düşük ve orta düzeylerdeki karaciğer hasarları üzerindeki immünopatolojik rolünün sınırlı olduğuna işaret etmektedir.

Keywords

Hepatitis B virüs, Kronik Hepatit B, Fibrozis evre, İnterlökin-17

Supporting Institution

İstanbul Üniversitesi Bilimsel Araştırma Projeleri

Project Number

30611

Thanks

İstanbul Tıp Fakültesi Gastroenterohepatoloji Bilim Dalında görevli Sayın Hemşire Nilay ARABACI ve Sayın Hemşire Derya KAYA ile İstanbul Tıp Fakültesi Radyoloj ABD Girişimsel Radyoloji Biriminde görevli Sayın Hemşire Arzu ÖRENTEL’e bu projenin gerçekleştirilmesi aşamasındaki bilimsel duyarlılıkları, katkıları ve emeklerinden dolayı şükranlarımı sunarım.

IMPLICATION OF SERUM IL-17 LEVELS IN CHRONIC HEPATITIS B PATIENTS

Abstract

Objective: Natural course and clinical outcomes of chronic hepatitis B virus (HBV) infection is closely releated to immun responses of host and viral factors. Studies have suggested that there is strongly correlation between the level of IL-17 and especially of advanced liver damages associated with HBV. Role of IL-17 on natural course of chronic hepatitis B disease is yet to be fully clarified. This study aimed to evaluate the correlation between serum IL-17 levels with histological, virological, serological and biochemical data of chronic hepatitis B patients. Material and Method: Simultaneous liver biopsy and blood samples were obtained from 88 with chronic HBV enfection. Ishak modified histological activity index system was used for histological evaluation.The patients were divided into three groups taking into account by scoring as none/mild (F0-1), moderate / severe (F2-4) and cirrhosis (F5-6) according to the stages of fibrosis (F). It was analyzed using enzyme-linked immunosorbent assay (ELISA) to IL-17 levels in serum samples. Results: A total of 88 CHB patients were evaluated, with an average age of 41.77±13.39, 43 male and 45 female. Fibrosis stage of 64 (72.7%) of the patients included in the study was determined as F2-4. There were no statistically significant correlation between serum IL-17 levels with histological, virological, serological and biochemical data of patients. Conclusion: These data indicate that the immunopathological role of IL-17 on HBV-related especially low and moderate liver injuries are limited.

Keywords

Hepatitis B virus, Chronic hepatitis B, Fibrosis stage, Interleukin-17

Project Number

30611

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