AGMATİNİN GENETİK BİR ABSANS EPİLEPSİ MODELİNDE DİKEN-VE-DALGA DEŞARJLAR ÜZERİNE ETKİSİ

Abstract

Amaç: Deneysel modellerde yapılan araştırmalar ekzojen agmatinin anti-konvülzan etkilerinin olduğunu göstermiştir. Ancak, agmatinin absans epilepsisi üzerindeki etkisine dair herhangi bir veri bulunmamaktadır. Bu çalışmada, agmatinin absans epilepsinin genetik bir modeli olan Wistar Albino Glaxo Rijswijk (WAG/ Rij) ırkı sıçanlarda Diken-ve-Dalga Deşarjlar (DDD) üzerine olan etkilerini araştırdık. Gereç ve Yöntem: Yetişkin WAG/Rij sıçanlarda periton içine 3 farklı doz agmatin (20, 40 ya da 80 mg/kg) ya da serum fizyolojik uygulandı ve 2,5 saat boyunca elektroensefalografi (EEG) kaydedildi. Elde edilen EEG kayıtlarında DDD’lerin sayısı, ortalama ve kümülatif süreleri değerlendirildi. DDD’lerin frekans özellikleri, etkili agmatin dozundan önce ve sonra, DDD aktivitesinin ilk iki saniyelik bölümlerinin ortalama güç spektrumları hesaplanarak karşılaştırıldı. Bulgular: WAG/Rij sıçanlara sistemik olarak uygulanan 80 mg/kg agmatin DDD’lerin sayısını ve kümülatif süresini serum fizyolojik uygulanan gruba göre geçici olarak arttırdı. Ancak, agmatinin bu etkisi DDD’lerin frekans özelliklerindeki bir değişiklikle ilişki bulunmadı. Daha düşük dozlarda uygulanan agmatin (20 or 40 mg/ kg) ise DDD’lerin sayı ve süresine değişikliğe yol açmadı. Sonuç: Yüksek doz agmatin WAG/Rij ırkı sıçanlarda DDD insidansını geçiçi olarak arttırmaktadır. Bu sonuçlar, agmatinin absans epilepsisi hastalarında adrenerjik, serotonerjik ve AMPA reseptörleri aracılığı ile olası bir kötüleştirici etki gösterebileceğini işaret etmektedir.

Keywords

• Agmatin
• absans epilepsi
• wistar albino glaxo rijswijk ırkı sıçanlar

Project Number

Project number: 2014/102HD

THE EFFECT OF AGMATINE ON SPIKE-AND-WAVE DISCHARGES IN A GENETIC MODEL OF ABSENCE EPILEPSY

Abstract

Objective: Studies on the actions of exogenous agmatine in experimental models have shown its anti-convulsant effects. However, there are no findings regarding the influence of agmatine on absence epilepsy. Therefore, we investigated the effects of the agmatine in the occurrence of spike-and-wave discharges (SWDs) in the Wistar Albino Glaxo Rijswijk rats (WAG/Rij) model of genetic absence epilepsy. Materials and Methods: Three different doses (20, 40 or 80 mg/kg) of agmatine or saline were administered intraperitoneally to the adult WAG/Rij rats, and electroencephalography (EEG) was recorded for 2.5 hours. The number and the mean and cumulative durations of SWDs were measured. The SWD frequency characteristics were quantified by means of average power-spectra of the first 2nd segments of the SWD complexes before and after the effective dose of agmatine. Results: Agmatine, administered 80 mg/kg systemically, temporarily increased the number and cumulative duration of SWDs compared with saline injected WAG/Rij rats. This effect of agmatine, however, was not associated with any change in the frequency characteristics of the SWD complexes. There was no effect on neither the incidence nor the cumulative duration of SWDs when agmatine administered in lower doses (20 or 40 mg/kg). Conclusion: Higher doses of agmatine temporarily and slightly increase the incidence of SWDs in WAG/Rij rats, which may suggest its possible aggravating activity in absence epilepsy patients through its activity on adrenergic, serotonergic and AMPA receptors.

Keywords

• Agmatine
• absence epilepsy
• wistar albino glaxo rijswijk rats

Supporting Institution

Scientific Research Projects Coordination Unit of Kocaeli University

Project Number

Project number: 2014/102HD

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