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KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ

Year 2020, Volume: 83 Issue: 3, 247 - 253, 29.06.2020

Abstract

üksek yağlı diyetin (YYD) neden olduğu oksidatif stresin testis fonksiyonlarını olumsuz yönde etkilediği bilinmektedir. Zerdeçal baharatının aktif bileşeni olan kurkuminin oksidan-antioksidan denge üzerine olumlu etkileri olduğu gösterilmiştir. Bu çalışmanın amacı; YYD birlikte alınan kurkuminin testiste oksidan-antioksidan denge üzerine olan etkisini incelemektir. Gereç ve Yöntem: Sprague-Dawley erkek sıçanlar dört gruba ayrılmış ve 16 hafta süreyle beslenmiştir. Birinci gruba kalorisinin %10’unun yağdan karşılandığı normal diyet, ikinci gruba kalorisinin %60’ının yağdan karşılandığı YYD, üçüncü gruba YYD’e karıştırılmış kurkumin (1g/kg yem) ve dördüncü gruba sadece normal diyet+kurkumin (1g/kg yem) verilmiştir. Deney süresi sonunda testis dokusu homojenize edilerek postmitokondriyal ve mitokondriyal fraksiyonlara ayrılmış; testiste reaktif oksijen türleri (ROS) düzeyleri florometrik yöntemle, malondialdehit (MDA) ve glutatyon (GSH) düzeyleri ile glutatyon peroksidaz (GPx), süperoksit dismütaz (SOD) ve glutatyon transferaz (GST) aktiviteleri spektrofotometrik yöntemlerle ölçülmüştür. Bulgular: YYD uygulaması, testiste postmitokondriyal ve mitokondriyal MDA düzeylerini arttırmış; sitozolik GSH düzeylerini ise azaltmıştır. YYD+Kurkumin uygulaması YYD’e bağlı artış gösteren MDA düzeylerini düşürmüş; GSH düzeyini ve GST aktivitesini ise arttırmıştır. Ancak sadece kurkumin uygulaması oksidan-antioksidan göstergeleri etkilememiştir. ROS düzeyleri ile SOD ve GPx aktiviteleri ise gruplar arasında anlamlı değişim göstermemiştir. Sonuç: Bulgularımız, YYD ile birlikte alınan kurkuminin testiküler oksidan-antioksidan dengeyi koruyucu olabileceğini göstermektedir. Ancak bu etkinin moleküler mekanizmalarının incelenmesi için ileri çalışmalar yapılması gerekmektedir.

References

  • 1. Liu Y, Ding Z. Obesity, a serious etiologic factor for male subfertility in modern society. Reproduction 2017;154(4):12331.
  • 2. Gujjala S, Putakala M, Gangarapu V, Nukala S, Bellamkonda R, Ramaswamy R, Desireddy S. Protective effect of Caralluma fimbriata against high-fat diet induced testicular oxidative stress in rats. Biomed Pharmacother 2016;83:16776.
  • 3. Nakamura S, Takamura T, Matsuzawa-Nagata N, Takayama H, Misu H, Noda H, et al. Palmitate induces insulin resistance in H4IIEC3 hepatocytes through reactive oxygen species produced by mitochondria. J Biol Chem 2009;284:1480918.
  • 4. Webster NR, Nunn JF. Molecular structure of free radicals and their importance in biological reactions. Br J Anaesth 1988;60(1):98-108.
  • 5. Rato L, Alves MG, Cavaco JE, Oliveira PF. High-energy diets: a threat for male fertility? Obesity Reviews 2014;15:9961007.
  • 6. Dohle GR, Diemer T, Giwercman A, Jungwirth A, Kopa Z, Krausz C. Guidelines on Male Infertility. E.A.U. 2010:6-8.
  • 7. Mortazavi M, Salehi I, Alizadeh Z, Vahabian M, Roushandeh AM. Protective Effects of Antioxidants on Sperm Parameters and Seminiferous Tubules Epithelium in High Fat-fed Rats. J Reprod Infertil 2014;15(1):22-8.
  • 8. Chen H, Zhao HX, Huang XF, Chen GW, Yang ZX, Sun WJ, et al. Does high load of oxidants in human semen contribute to male factor infertility? Antioxid Redox Signal 2012;16(8):754-9.
  • 9. Sharma RA, Gesch er AJ, Steward WP. Curcumin: the story so far. Eur J Cancer 2005;41:1955-68.
  • 10. Tanrıkulu-Küçük S, Başaran-Küçükgergin C, Seyithanoğlu M, Doğru-Abbasoğlu S, Koçak H, Beyhan-Özdaş Ş, Önerİyidoğan Y. Effect of dietary curcumin and capsaicin on testicular and hepatic oxidant-antioxidant status in rats fed a high-fat diet. Appl Physiol Nutr Metab 2019;3:1-9.
  • 11. Öner-İyidoğan Y, Tanrıkulu-Küçük S, Seyithanoğlu M, Koçak H, Doğru-Abbasoğlu S, Aydin AF, et al. Effect of curcumin on hepatic heme oxygenase 1 expression in high fat diet fed rats: is there a triangular relationship? Can J Physiol Pharmacol 2014;92:805-12.
  • 12. Aggarwal BB, Harikumar KB. Potential therapeutic eff ects of curcumin, the anti-infl ammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol 2009;41:40-59.
  • 13. Goel A, Jhurani S, Aggarwal BB. Multi-targeted therapy by curcumin: How spicy is it? Molecular Nutrition and Food Research 2008;52:1010-30.
  • 14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • 15. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1979;61:882-8.
  • 16. Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium deficient rat liver. Biochem Biophys Res Commun 1976;71:952-8.
  • 17. Habig WH, Jacoby WB. Assays for differentiation of glutathione S-transferases. Methods Enzymol 1981;77: 398405.
  • 18. Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of copper status in rats ingesting lead acetate. Toxicol Appl Pharmacol 1986;82:512-20.
  • 19. Lu J, Wu DM, Zheng YL, Hu B, Cheng W, Zhang ZF, Li MQ. Troxerutin counteracts domoic acid-induced memory deficits in mice by inhibiting CCAAT/enhancer binding protein β-mediated inflammatory response and oxidative stress. J Immunol 2013;190:3466-79.
  • 20. Wang H, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 1999;27:612-6.
  • 21. Smith S. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB J 1994; 8:1248-59.
  • 22. Kanter M, Aktas C, Erboga M. Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin-induced diabetic rats.Mol Nutr Food Res 2013;57(9):1578-85.
  • 23. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 1991;11(1):81-128.
  • 24. Xu DP, Li Y, Meng X, Zhou T, Zhou Y, Zheng J, et al. Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. Int J Mol Sci 2017;18:96.
  • 25. Alappat L, Awad AB. Curcumin and obesity: evidence and mechanisms. Nutr Rev 2010;68(12):729-38.
  • 26. Zhao L, Gu Q, Xiang L, Dong X, Li H, Ni J, et al. Curcumin inhibits apoptosis by modulating Bax/Bcl-2 expression and alleviates oxidative stress in testes of streptozotocininduced diabetic rats. Ther Clin Risk Manag 2017;28:1099105.
  • 27. Pastore A, Federici G, Bertini E, Piemonte F. Analysis of glutathione: implication in redox and detoxification. Clin Chim Acta 2003;333(1):19-39.
  • 28. Sudhakara G, Mallaiah P, Sreenivasulu N, Sasi Bhusana Rao B, Rajendran R, Saralakumari D. Beneficial effects of hydroalcoholic extract of Caralluma fimbriata against high-fat diet induced insulin resistance and oxidative stress in Wistar male rats. J Physiol Biochem 2014;70:311-20.
  • 29. Lee SJ, Choi SK, Seo JS. Grape skin improves antioxidant capacity in rats fed a high fat diet. Nutr Res Pract 2009;3(4):279-85.
  • 30. Dikmen M, Kaya-Tilki E, Engur S, Ozturk Y. Neuritogenic activity of epigallocatechin gallate and curcumin combination on rat adrenal pheochromocytoma cells. Fresenius Environ Bull 2017;26:4726-33.

EFFECT OF DIETARY CURCUMIN ON OXIDANT-ANTIOXIDANT STATUS IN TESTIS TISSUES OF RATS FED A HIGH-FAT DIET

Year 2020, Volume: 83 Issue: 3, 247 - 253, 29.06.2020

Abstract

Objective: High fat diet (HFD) induced oxidative stress is known to adversely affect testicular functions. Curcumin, which is an active ingredient of turmeric spice, has been shown to reduce lipid peroxidation and stimulate cellular antioxidant enzymes. The aim of this study is to investigate the effect of dietary curcumin on the testicular oxidant-antioxidant status in rats fed on a HFD. Material and Method: Male Sprague-Dawley rats were divided into four groups. Group 1 was fed with a control diet (10% of total calories from fat). Group 2 was fed with a HFD (60% of total calories from fat). Groups 3 and 4 received a HFD and a control diet with curcumin (1g/kg diet; w/w) respectively for 16 weeks. The testis tissue was homogenized and divided into postmitochondrial and mitochondrial fractions and the reactive oxygen species (ROS) levels were measured with the fluorometric method. The malondialdehyde (MDA) and glutathione (GSH) levels and glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione transferase (GST) activities were measured with spectrophotometric methods. Results: HFD supplementation increased postmitochondrial and mitochondrial MDA levels and decreased cytosolic GSH levels in testis. MDA levels decreased with curcumin supplementation. Moreover, GSH levels and GST activity increased. However, curcumin supplementation alone did not affect oxidant-antioxidant status. ROS levels, SOD and GPx activities did not change significantly between the groups. Conclusion: Our data has shown that curcumin supplementation with HFD may prevent testicular oxidant damage. However, further studies are needed to investigate the underlying molecular mechanisms.

References

  • 1. Liu Y, Ding Z. Obesity, a serious etiologic factor for male subfertility in modern society. Reproduction 2017;154(4):12331.
  • 2. Gujjala S, Putakala M, Gangarapu V, Nukala S, Bellamkonda R, Ramaswamy R, Desireddy S. Protective effect of Caralluma fimbriata against high-fat diet induced testicular oxidative stress in rats. Biomed Pharmacother 2016;83:16776.
  • 3. Nakamura S, Takamura T, Matsuzawa-Nagata N, Takayama H, Misu H, Noda H, et al. Palmitate induces insulin resistance in H4IIEC3 hepatocytes through reactive oxygen species produced by mitochondria. J Biol Chem 2009;284:1480918.
  • 4. Webster NR, Nunn JF. Molecular structure of free radicals and their importance in biological reactions. Br J Anaesth 1988;60(1):98-108.
  • 5. Rato L, Alves MG, Cavaco JE, Oliveira PF. High-energy diets: a threat for male fertility? Obesity Reviews 2014;15:9961007.
  • 6. Dohle GR, Diemer T, Giwercman A, Jungwirth A, Kopa Z, Krausz C. Guidelines on Male Infertility. E.A.U. 2010:6-8.
  • 7. Mortazavi M, Salehi I, Alizadeh Z, Vahabian M, Roushandeh AM. Protective Effects of Antioxidants on Sperm Parameters and Seminiferous Tubules Epithelium in High Fat-fed Rats. J Reprod Infertil 2014;15(1):22-8.
  • 8. Chen H, Zhao HX, Huang XF, Chen GW, Yang ZX, Sun WJ, et al. Does high load of oxidants in human semen contribute to male factor infertility? Antioxid Redox Signal 2012;16(8):754-9.
  • 9. Sharma RA, Gesch er AJ, Steward WP. Curcumin: the story so far. Eur J Cancer 2005;41:1955-68.
  • 10. Tanrıkulu-Küçük S, Başaran-Küçükgergin C, Seyithanoğlu M, Doğru-Abbasoğlu S, Koçak H, Beyhan-Özdaş Ş, Önerİyidoğan Y. Effect of dietary curcumin and capsaicin on testicular and hepatic oxidant-antioxidant status in rats fed a high-fat diet. Appl Physiol Nutr Metab 2019;3:1-9.
  • 11. Öner-İyidoğan Y, Tanrıkulu-Küçük S, Seyithanoğlu M, Koçak H, Doğru-Abbasoğlu S, Aydin AF, et al. Effect of curcumin on hepatic heme oxygenase 1 expression in high fat diet fed rats: is there a triangular relationship? Can J Physiol Pharmacol 2014;92:805-12.
  • 12. Aggarwal BB, Harikumar KB. Potential therapeutic eff ects of curcumin, the anti-infl ammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol 2009;41:40-59.
  • 13. Goel A, Jhurani S, Aggarwal BB. Multi-targeted therapy by curcumin: How spicy is it? Molecular Nutrition and Food Research 2008;52:1010-30.
  • 14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • 15. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1979;61:882-8.
  • 16. Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium deficient rat liver. Biochem Biophys Res Commun 1976;71:952-8.
  • 17. Habig WH, Jacoby WB. Assays for differentiation of glutathione S-transferases. Methods Enzymol 1981;77: 398405.
  • 18. Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of copper status in rats ingesting lead acetate. Toxicol Appl Pharmacol 1986;82:512-20.
  • 19. Lu J, Wu DM, Zheng YL, Hu B, Cheng W, Zhang ZF, Li MQ. Troxerutin counteracts domoic acid-induced memory deficits in mice by inhibiting CCAAT/enhancer binding protein β-mediated inflammatory response and oxidative stress. J Immunol 2013;190:3466-79.
  • 20. Wang H, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 1999;27:612-6.
  • 21. Smith S. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB J 1994; 8:1248-59.
  • 22. Kanter M, Aktas C, Erboga M. Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin-induced diabetic rats.Mol Nutr Food Res 2013;57(9):1578-85.
  • 23. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 1991;11(1):81-128.
  • 24. Xu DP, Li Y, Meng X, Zhou T, Zhou Y, Zheng J, et al. Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. Int J Mol Sci 2017;18:96.
  • 25. Alappat L, Awad AB. Curcumin and obesity: evidence and mechanisms. Nutr Rev 2010;68(12):729-38.
  • 26. Zhao L, Gu Q, Xiang L, Dong X, Li H, Ni J, et al. Curcumin inhibits apoptosis by modulating Bax/Bcl-2 expression and alleviates oxidative stress in testes of streptozotocininduced diabetic rats. Ther Clin Risk Manag 2017;28:1099105.
  • 27. Pastore A, Federici G, Bertini E, Piemonte F. Analysis of glutathione: implication in redox and detoxification. Clin Chim Acta 2003;333(1):19-39.
  • 28. Sudhakara G, Mallaiah P, Sreenivasulu N, Sasi Bhusana Rao B, Rajendran R, Saralakumari D. Beneficial effects of hydroalcoholic extract of Caralluma fimbriata against high-fat diet induced insulin resistance and oxidative stress in Wistar male rats. J Physiol Biochem 2014;70:311-20.
  • 29. Lee SJ, Choi SK, Seo JS. Grape skin improves antioxidant capacity in rats fed a high fat diet. Nutr Res Pract 2009;3(4):279-85.
  • 30. Dikmen M, Kaya-Tilki E, Engur S, Ozturk Y. Neuritogenic activity of epigallocatechin gallate and curcumin combination on rat adrenal pheochromocytoma cells. Fresenius Environ Bull 2017;26:4726-33.
There are 30 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section RESEARCH
Authors

Muhammed Seyithanoğlu This is me 0000-0002-8027-7549

Sevda Tanrıkulu Küçük This is me 0000-0001-9446-4399

Yıldız Öner İyidoğan This is me 0000-0001-6956-8794

Hikmet Koçak This is me 0000-0003-1720-9102

Parvana Mikailova This is me 0000-0002-6883-9051

Abdurrahman Fatih Aydın This is me 0000-0002-2595-0833

Publication Date June 29, 2020
Submission Date July 7, 2019
Published in Issue Year 2020 Volume: 83 Issue: 3

Cite

APA Seyithanoğlu, M., Tanrıkulu Küçük, S., Öner İyidoğan, Y., Koçak, H., et al. (2020). KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ. Journal of Istanbul Faculty of Medicine, 83(3), 247-253.
AMA Seyithanoğlu M, Tanrıkulu Küçük S, Öner İyidoğan Y, Koçak H, Mikailova P, Aydın AF. KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ. İst Tıp Fak Derg. June 2020;83(3):247-253.
Chicago Seyithanoğlu, Muhammed, Sevda Tanrıkulu Küçük, Yıldız Öner İyidoğan, Hikmet Koçak, Parvana Mikailova, and Abdurrahman Fatih Aydın. “KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ”. Journal of Istanbul Faculty of Medicine 83, no. 3 (June 2020): 247-53.
EndNote Seyithanoğlu M, Tanrıkulu Küçük S, Öner İyidoğan Y, Koçak H, Mikailova P, Aydın AF (June 1, 2020) KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ. Journal of Istanbul Faculty of Medicine 83 3 247–253.
IEEE M. Seyithanoğlu, S. Tanrıkulu Küçük, Y. Öner İyidoğan, H. Koçak, P. Mikailova, and A. F. Aydın, “KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ”, İst Tıp Fak Derg, vol. 83, no. 3, pp. 247–253, 2020.
ISNAD Seyithanoğlu, Muhammed et al. “KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ”. Journal of Istanbul Faculty of Medicine 83/3 (June 2020), 247-253.
JAMA Seyithanoğlu M, Tanrıkulu Küçük S, Öner İyidoğan Y, Koçak H, Mikailova P, Aydın AF. KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ. İst Tıp Fak Derg. 2020;83:247–253.
MLA Seyithanoğlu, Muhammed et al. “KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ”. Journal of Istanbul Faculty of Medicine, vol. 83, no. 3, 2020, pp. 247-53.
Vancouver Seyithanoğlu M, Tanrıkulu Küçük S, Öner İyidoğan Y, Koçak H, Mikailova P, Aydın AF. KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ. İst Tıp Fak Derg. 2020;83(3):247-53.

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