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Year 2015, Volume: 45 Issue: 2, 165 - 179, 22.11.2016

Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2 Carola Hunte 4, Z. Petek Çakar1,2

Abstract

Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism

Year 2015, Volume: 45 Issue: 2, 165 - 179, 22.11.2016

Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2 Carola Hunte 4, Z. Petek Çakar1,2

Abstract

Abstract: The yeast Saccharomyces cerevisiae is a simple eukaryote and an excellent model organism for molecular biology. In this study, a NaCl-resistant S. cerevisiae mutant obtained by inverse metabolic engineering was used as a model to investigate responses and resistance behavior to NaCl, LiCl, KCl, TMA, spermine and sorbitol stresses., at physiological and genetic levels. The physiological spot test results revealed that the NaCl-resistant yeast mutant showed higher resistance to LiCl and NaCl. Gene expression analysis by qRT-PCR revealed that ENA6 and NHA1 genes of the mutant were induced in the absence and presence of LiCl and NaCl. The dysfunction of Na+/H+ antiporters are related to several diseases such as hypertension, epilepsy, postischemic myocardial arrhythmia, gastric and kidney disease, diarrhea, and glaucoma. Thus, the NaCl-resistant yeast mutant could be used to understand cellular sodium and lithium resistance mechanisms and the function of Na+/H+ antiporters also in higher eukaryotic organisms, including humans.
Key words: Saccharomyces cerevisiae, stress resistance, salt-resistance , lithium resistance, NHA1

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Authors

Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2 Carola Hunte 4, Z. Petek Çakar1,2

Publication Date November 22, 2016
Published in Issue Year 2015 Volume: 45 Issue: 2

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APA Carola Hunte 4, Z. Petek Çakar1,2, Ş. H. T. 1,2,3,ceren A. 1,2. (2016). Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University, 45(2), 165-179.
AMA Carola Hunte 4, Z. Petek Çakar1,2 ŞHT 1,2,3,ceren A 1,2. Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University. January 2016;45(2):165-179.
Chicago Carola Hunte 4, Z. Petek Çakar1,2, Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2. “Physiological and Genetic Analysis of Cellular Sodium and Lithium Response Resistance Behavior Using the Yeast Saccharomyces Cerevisiae As a Model Organism”. Journal of Faculty of Pharmacy of Istanbul University 45, no. 2 (January 2016): 165-79.
EndNote Carola Hunte 4, Z. Petek Çakar1,2 ŞHT 1,2,3,ceren A 1,2 (January 1, 2016) Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University 45 2 165–179.
IEEE Ş. H. T. 1,2,3,ceren A. 1,2 Carola Hunte 4, Z. Petek Çakar1,2, “Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism”, Journal of Faculty of Pharmacy of Istanbul University, vol. 45, no. 2, pp. 165–179, 2016.
ISNAD Carola Hunte 4, Z. Petek Çakar1,2, Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2. “Physiological and Genetic Analysis of Cellular Sodium and Lithium Response Resistance Behavior Using the Yeast Saccharomyces Cerevisiae As a Model Organism”. Journal of Faculty of Pharmacy of Istanbul University 45/2 (January2016), 165-179.
JAMA Carola Hunte 4, Z. Petek Çakar1,2 ŞHT 1,2,3,ceren A 1,2. Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University. 2016;45:165–179.
MLA Carola Hunte 4, Z. Petek Çakar1,2, Ş. Hande Tekarslan 1,2,3,ceren Alkım 1,2. “Physiological and Genetic Analysis of Cellular Sodium and Lithium Response Resistance Behavior Using the Yeast Saccharomyces Cerevisiae As a Model Organism”. Journal of Faculty of Pharmacy of Istanbul University, vol. 45, no. 2, 2016, pp. 165-79.
Vancouver Carola Hunte 4, Z. Petek Çakar1,2 ŞHT 1,2,3,ceren A 1,2. Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism. Journal of Faculty of Pharmacy of Istanbul University. 2016;45(2):165-79.