PURIFICATION AND ANTIOXIDANT ACTIVITY OF ALOE VERA LEAF LECTIN

Abstract

Aloe vera (L.) Burm. fil. lektinin saflaştırılması için, yeni ve hızlı bir kromatografi yöntemi olan, ovalbumin bağlı-siyanojen bromür (CNBr) ile aktive edilmiş Sefaroz 4B, afinite kromatografisi tanıtılmaktadır. Lektin, kromatografi sonucunda 60 kez saflaştırıldı. Poliakrilamid disk jel elektroforezi (PAGE) ile homojen olduğu gözlemlenen lektinin molekül ağırlığı bu yöntemle, 45 000 kD olarak saptandı. Sodyum dodesil sülfat (SDS)-PAGE ile altbirim molekül ağırlığı 14 400 kDa olarak belirlenen lektinin, üç alt birimden oluştuğu ve bunun da daha önce tarafımızdan kısmen saflaştırılmış ve karakterize edilmiş olan Aloctin I ile uyum içinde olduğu sonucuna varıldı. Saflaştırılan lektin, DPPH· radikal süpürme tayini ile değerlendirildiğinde antioksidan etki göstermedi.

Keywords

• Aloe vera
• lectin
• purification
• affinity chromatography
• antioxidant activity

PURIFICATION AND ANTIOXIDANT ACTIVITY OF ALOE VERA LEAF LECTIN

Abstract

A new and rapid affinity chromatography method based on cyanogen bromide (CNBr)-activated Sepharose 4B bound-ovalbumin is presented for the purification of the main lectin present in Aloe vera (L.) Burm. fil. The lectin was purified 60 fold to apparent homogeneity in native polyacrylamide disc gel electrophoresis (PAGE) showing an apparent molecular weight of 45000 kDa. The fact that sodium dodecyl sulfate (SDS)-PAGE gave a subunit molecular weight of 14 400 kDa tends to propose that the lectin is composed of three subunits and thus is in agreement with Aloctin I previously partially purified and characterized by us. The lectin did not exhibit antioxidant effect as assessed by the DPPH· radical-scavenging assay.

Keywords

• Aloe vera
• lectin
• purification
• affinity chromatography
• antioxidant activity

References

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