CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY

Abstract

In this study, a simple, rapid and accurate capillary electrophoresis method has been developed for the determination of lactulose and mannitol in urine for estimating the intestinal permeability. The urine samples were prepared to analyze by using solid phase extraction. For analysis a background electrolyte (BGE) consisted of 6 mmol/L sorbic acid, 1.25mmol/L hexadecyltrimeth ylammoniumbromide (HDTMA), 50 mmol/L LiOH (pH 12.5) was used. Separation was performed at -5 kV over 20 min within the fused capillary column (45 cm, 50 µm I.D.) at 20 °C. The method developed was applied a series of urine sample to measure the lactulose/mannitol concentration rate.

Keywords

• Lactulose
• Mannitol
• Determination
• Permeability
• Capillary electrophoresis

CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY

Abstract

Bu çalışmada intestinal geçirgenliğin incelenmesi amacıyla idrarda laktuloz ve mannitol tayini için basit, hızlı ve doğru bir kapiler elektroforez yöntemi geliştirilmiştir. İdrar örnekleri katı faz özütleme yöntemi ile analize hazırlanmıştır. Analizler için 6 mmol/L sorbik asit, 1.25 mmol/L hekzadesil trimetil amonyum bromür (HDTMA), 50 mmol/ L LiOH içeren pH sı 12.5 e karşılık gelen destek elektrolit kullanılmıştır. Ayrım 45 cm 50 µm iç çaplı fused silika kolonda 20 °C sıcaklıkta -5 kV ayırma potansiyeli kullanılarak 20 dk içerisinde gerçekleştirilmiştir. Geliştirilen yöntem laktuloz / mannitol derişim oranlarını ölçmek için bir seri idrar numunesine uygulanmıştır.

Keywords

• Laktuloz
• Mannitol
• Tayin
• Geçirgenlik
• Kapiler elektroforez

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