BIOLOGICAL ACTIVITIES AND PHENOLIC-FLAVONOID CONTENTS OF BILBERRY EXTRACTS

Abstract

Bu çalışmada, yaban mersini bitkisinin çiçek kısımlarının petrol eteri, diklorometan, metanol ve sıcak su ekstreleri hazırlandı. Bu ekstrelerin toplam fenolik madde miktarları pirokatekole eşdeğer, toplam flavonoit madde miktarları kersetine eşdeğer olarak belirlendi. Antioksidan tayin yöntemleri olarak, DPPH serbest radikal ve ABTS katyon radikali giderim, β-karoten renk açılım ve CUPRAC yöntemleri kullanıldı. Metanol ekstresi bu yöntemlerin ikisinde en yüksek antioksidan aktiviteyi gösterdi. Ekstrelerin antikolinesteraz aktiviteleri, asetilkolinesteraz ve bütirilkolinesteraz enzimlerinin inhibisyonuna dayanan Ellman yöntemi ile belirlendi. Metanol ekstresi bütirilkolinesteraza karşı en yüksek aktiviteyi gösterdi. Yaban mersini bitkisinin çiçek kısımlarının ekstrelerinin antioksidan ve antikolinesteraz aktiviteleri ilk kez bu çalışma ile belirlendi.

Keywords

• Bilberry
• Antioxidant activity
• Anticholinesterase activity

BIOLOGICAL ACTIVITIES AND PHENOLIC-FLAVONOID CONTENTS OF BILBERRY EXTRACTS

Abstract

In this study, the petroleum ether, dichloromethane, methanol and hot water extracts were prepared from bilberry blooms. Total phenolic and flavonoid contents of these extracts were determined as pyrocatechol and quercetin equivalents, respectively. The antioxidant activity of the extracts was investigated by using four methods including DPPH free radical and ABTS cation radical scavenging, β-carotene bleaching and cupric reducing antioxidant capacity (CUPRAC) assays. The methanol extract showed the highest antioxidant activity in two assays. The anticholinesterase effect of the extracts was determined by using Ellman method via inhibition of acetyl- (AChE) and butyryl-cholinesterase (BChE) enzymes. The strongest inhibition against BChE was observed for the methanol extract. The antioxidant and anticholinesterase potential of bilberry bloom extracts was determined for the first time.

Keywords

• Bilberry
• Antioxidant activity
• Anticholinesterase activity

References

1. Camire, M.E., In: Meskin, M.S., Bidlack, W.R., Davies, A.J., Omaye, S.T. (Eds.), Phytochemicals in Nutrition and Health. CRC Press, Boca Raton, 289 (2002).
2. Valentova, K., Ulrichova , J., Cvak, L., Sˇimanek, V. Food Chem, 101, 912–917 (2007).
3. Yao, Y., Vieira, A., Neurotoxicol, 28, 93–100 (2007).
4. Szakiel, A., Voutquenne-Nazabadioko, L., Henry, M., Phytochem Lett, 4, 138−143 (2011).
5. Giovanelli, G., Buratti, S., Food Chem, 112, 903-908 (2009).
6. Wolfe, K.L., Liu, R.H., J Agric Food Chem, 55, 8896-8907 (2007).
7. Mazza, G., Miniati, E., Introduction., in: Anthocyanins in fruits, vegetables and grain CRC Press, Inc.Boca Raton, Florida, 1–28 (1993).
8. Slinkard, K., Singleton, V.L., Am J Enol Vit should be written in, 28, 49–55 (1977).

9. Park, Y.S., Jung, S.T., Kang, S.G., Heo, B.K., Arancibia-Avila, P., Toledo, F., Food Chem, 107, 640–648 (2008).
10. Blois, M.S,. Nature, 181, 1199-1200 (1958).
11. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice- Evans, C., Free Radical Bio Med, 26, 1231–1237 (1999).
12. Miller, H.E., J Am Oil Chem Soc, 48, 91 (1971).
13. Apak, R., Guclu, K., Ozyurek, M., Karademir, S.E., J Agric Food Chem, 52, 7970-7981 (2004).
14. Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M., Biochem. Pharmacol. 7, 88–95 (1961).