In this study, a simple, rapid and accurate spectrophotometric method has been
developed for the assay of oseltamivir phosphate (OSP). The proposed method based on
the charge-transfer reactions of oseltamivir, as n-electron donor, with 7,7,8,8-
tetracyanoquinodimethane (TCNQ), as π-acceptors to give highly colored complexes.
The experimental conditions such as reagent concentration, reaction solvent and time
have been carefully optimized to achieve the highest sensitivity. Beer's law is obeyed
over the concentration range of 3–30 μg/mL with correlation coefficient 0.9999. The
method was applied successfully to the determination of this drug in capsules. The mean
recovery for the commercial capsules was 100.03%. The suggested method could be
used for the determination of OSP in pure and capsules being sensitive, simple and
selective.
16. R. Foster, Organic Charge-Transfer Complexes, Academic Press, New York
(1969).
17. Cağlar S, Oztunç A. J AOAC Int. 90(2):372-5 (2007).
18. Aydoğmuş Z, Barla A. J AOAC Int. 92:131-7(2009).
19. The European Agency for the Evaluation of Medicinal Products, ICH Topic
Q2B Note for Guideline on Validation of Analytical Procedures, Methodology
GPMP/ICH/281/95, (1996).
A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES
Bu çalışmada, oseltamivir fosfatın tayini için basit, hızlı ve doğru bir
spektrofotometrik bir yöntem geliştirildi. Yöntem bir n-elektron verici olan
oseltamivirin bir π-alıcısı olan 7,7,8,8-tetrasiyanokinodimetan (TCNQ) ile oldukça
renkli bir kompleks oluşumuna dayanmaktadır. Analizi en yüksek hassasiyette
gerçekleştirmek için belirteç miktarı, reaksiyon çözeltisi, reaksiyon zamanı gibi
deneysel koşullar dikkatlice incelendi. Kalibrasyon eğrisinin 3–30 μg/mL derişim aralığında doğrusal olduğu görüldü (R= 0,9999). Geliştirilen yöntem ilacın
kapsullerdeki tayinine başarılı ile uygulandı. Ticari kapsüllerde ortalama geri kazınım %
100.03 olarak bulundu. Önerilen yöntemin, ilaçlarda rutin oseltamivir fosfat analizinde
kalite kontrol amacıyla kullanılabilecek kolay, hassas ve seçiciliği yüksek bir yöntem
olduğu belirlendi.
References
1. McClellan K, Perry C.M. Drugs 61: 263-283 (2001).
2. Nicholson K.G, Aoki F.Y., Osterhaus ADME, Trottier S, Carewicz O, Mercier
CH, Rode A, Kinnersley N, Ward P. Lancet 355:1845–1850 (2000).
3. Lindegårdh N., Hien T.T, Farrar J., Singhasivanon P., White N.J, Day N.P. J
Pharm
4. Biomed Anal. 42:430-433 (2006).
5. Joseph-Charles J., Geneste C., Laborde-Kummer E., Gheyouche R., Boudis H.,
Dubost J.P.
16. R. Foster, Organic Charge-Transfer Complexes, Academic Press, New York
(1969).
17. Cağlar S, Oztunç A. J AOAC Int. 90(2):372-5 (2007).
18. Aydoğmuş Z, Barla A. J AOAC Int. 92:131-7(2009).
19. The European Agency for the Evaluation of Medicinal Products, ICH Topic
Q2B Note for Guideline on Validation of Analytical Procedures, Methodology
GPMP/ICH/281/95, (1996).
Sarı, F., & Aydoğmuş, Z. (2011). A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES. Journal of Faculty of Pharmacy of Istanbul University(40), 47-55.
AMA
Sarı F, Aydoğmuş Z. A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES. Journal of Faculty of Pharmacy of Istanbul University. May 2011;(40):47-55.
Chicago
Sarı, Ferhat, and Zeynep Aydoğmuş. “A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES”. Journal of Faculty of Pharmacy of Istanbul University, no. 40 (May 2011): 47-55.
EndNote
Sarı F, Aydoğmuş Z (May 1, 2011) A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES. Journal of Faculty of Pharmacy of Istanbul University 40 47–55.
IEEE
F. Sarı and Z. Aydoğmuş, “A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES”, Journal of Faculty of Pharmacy of Istanbul University, no. 40, pp. 47–55, May 2011.
ISNAD
Sarı, Ferhat - Aydoğmuş, Zeynep. “A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES”. Journal of Faculty of Pharmacy of Istanbul University 40 (May 2011), 47-55.
JAMA
Sarı F, Aydoğmuş Z. A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES. Journal of Faculty of Pharmacy of Istanbul University. 2011;:47–55.
MLA
Sarı, Ferhat and Zeynep Aydoğmuş. “A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES”. Journal of Faculty of Pharmacy of Istanbul University, no. 40, 2011, pp. 47-55.
Vancouver
Sarı F, Aydoğmuş Z. A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF OSELTAMIVIR PHOSPAHATE IN BULK AND CAPSULES. Journal of Faculty of Pharmacy of Istanbul University. 2011(40):47-55.