EFFECTS OF TAURINE AND VITAMIN E ON LIPID PEROXIDATION, ANTIOXIDANT SYSTEM AND HISTOLOGICAL CHANGES IN WISTAR RAT LIVER AND KIDNEY AFTER SHORT-TERM EXPOSURE OF METHIOCARB

Year 2012, Volume: 2 Issue: 42(2), 83 - 102, 10.01.2014

S. Ozden S. Bolkent S. Gezgıncı-oktayoglu P. Arda-pırınccı A. Karatug B. Alpertunga S. Ozden1,*, S. Bolkent2, S. Gezginci-ok S. Ozden1,*, S. Bolkent2, S. Gezgıncı-oktayoglu2, P. Arda-pırınccı2, A. Karatug2, B. Alper

Abstract

The present work was designed to examine possible effects of methiocarb on lipid peroxidation, reduced glutathione (GSH), antioxidant enzymes such as superoxide distumtase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) ve glutathione reductase (GSH-Rd) and histological changes in Wistar albino rats after 5-day exposure. Moreover, we evaluated possible protective effects of vitamin E and taurine on methiocarb-induced oxidative damage. Fourty-eight rats were randomly divided into six groups as follows: control; methiocarb; vitamin E; vitamin E+methiocarb; taurine and taurine+methiocarb. 5-day exposure of methiocarb (1/10 of LD50) significantly increased lipid peroxidation in liver and kidney when compared to control group. Levels of GSH and activities of SOD and CAT were found to be decreased, while GSH-Px increased and GSH-Rd remained unchanged in rat tissues treated with methiocarb. Pre-treatment of vitamin E (100 mg/kg b.w.) and taurine (50 mg/kg b.w.) resulted in a significant decrease on lipid peroxidation, alleviating effects on GSH and antioxidant enzymes. Administration of methiocarb caused to the degenerative changes in liver and kidney tissues. Pre-treatment of vitamin E and taurine were partially protected both tissues against methiocarb-induced liver and kidney injury. In conclusion, the study has demonstrated that 5-day methiocarb exposure in Wistar rats caused oxidative damage on liver and kidney which were partly ameliorated by pre-treatment of vitamin E and taurine.

Keywords

Methiocarb, Taurine, Vitamin E, Lipid peroxidation, Antioxidant system, Histological changes

EFFECTS OF TAURINE AND VITAMIN E ON LIPID PEROXIDATION, ANTIOXIDANT SYSTEM AND HISTOLOGICAL CHANGES IN WISTAR RAT LIVER AND KIDNEY AFTER SHORT-TERM EXPOSURE OF METHIOCARB

Abstract

Bu çalışma, Wistar albino sıçanlara 5 günlük methiocarb maruziyetinin
lipid peroksidasyonu, indirgenmiş glutatyon (GSH), süperoksid dizmütaz
(SOD), katalaz (CAT), glutatyon peroksidaz (GSH-Px) ve glutatyon
redüktaz (GSH-Rd) gibi antioksidan enzimler ve histolojik değişiklikler
üzerine muhtemel etkilerinin araştırılması için planlandı. Buna ilave
olarak, methiocarb indüklü oksidatif hasar üzerine E vitamini ve taurininin
muhtemel koruyucu etkileri de araştırıldı. 48 adet sıçan randomize olarak
6 gruba ayrıldı: kontrol; methiocarb; E vitamini; E vitamini+metiocarb;
taurin ve taurin+metiyocarb. Kontrol grubuna kıyasla 5 günlük metiyokarb
maruziyetinin (1/10 LD50) karaciğer ve böbrekte lipid peroksidasyonunu
arttırdı. Metiyokarb maruziyeti sonucunda sıçan dokularında GSH seviyeleri
ve SOD ve CAT enzim aktivitelerinde düşüş ve GSH-Px aktivitesinde artış
gözlenirken, GSH-Rd aktivitesinde ise bir değişiklik gözlenmedi. E vitamini
(100 mg/kg) ve taurin (50 mg/kg) ile tedavi sonrasında lipid peroksidasyonda
belirgin bir düşüş ve GSH seviyelerinde ve antioksidan enzim aktivitelerinde
de düzelme olduğu gösterildi. Methiocarb maruziyetinin karaciğer ve böbrek
dokularında dejeneratif değişikliklere yol açtığı gösterildi ve E vitamini
ve taurin ile tedavi sonrasında her iki dokuda da meydana gelen histolojik
değişikliklerde kısmen iyileşme olduğu gösterildi. Sonuç olarak, 5 günlük
methiocarb maruziyetinin Wistar sıçanların karaciğer ve böbreklerinde
oksidatif hasara sebep olduğu ve bu hasarın da E vitamini ve taurin tedavisi
ile kısmen iyileşme gösterdiği sonucuna varılmıştır.

Keywords

Methiocarb, Taurin, E Vitamini, Lipid peroksidasyonu, Antioksidan enzimler, Histolojik değişiklikler

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