BIOLOGICAL ACTIVITIES AND PHENOLIC-FLAVONOID CONTENTS OF BILBERRY EXTRACTS

Year 2012, Volume: 2 Issue: 42(2), 103 - 111, 10.01.2014

İ. Hacıbekiroğlu U. Kolak* İ. Hacibekiroğlu, U. Kolak* I. Hacıbekiroğlu, U. Kolak*

Abstract

Bu çalışmada, yaban mersini bitkisinin çiçek kısımlarının petrol eteri,
diklorometan, metanol ve sıcak su ekstreleri hazırlandı. Bu ekstrelerin
toplam fenolik madde miktarları pirokatekole eşdeğer, toplam flavonoit
madde miktarları kersetine eşdeğer olarak belirlendi. Antioksidan tayin
yöntemleri olarak, DPPH serbest radikal ve ABTS katyon radikali giderim,
β-karoten renk açılım ve CUPRAC yöntemleri kullanıldı. Metanol ekstresi
bu yöntemlerin ikisinde en yüksek antioksidan aktiviteyi gösterdi. Ekstrelerin
antikolinesteraz aktiviteleri, asetilkolinesteraz ve bütirilkolinesteraz
enzimlerinin inhibisyonuna dayanan Ellman yöntemi ile belirlendi. Metanol
ekstresi bütirilkolinesteraza karşı en yüksek aktiviteyi gösterdi. Yaban mersini
bitkisinin çiçek kısımlarının ekstrelerinin antioksidan ve antikolinesteraz
aktiviteleri ilk kez bu çalışma ile belirlendi.

Keywords

Bilberry, Antioxidant activity, Anticholinesterase activity

BIOLOGICAL ACTIVITIES AND PHENOLIC-FLAVONOID CONTENTS OF BILBERRY EXTRACTS

Abstract

In this study, the petroleum ether, dichloromethane, methanol and hot water
extracts were prepared from bilberry blooms. Total phenolic and flavonoid
contents of these extracts were determined as pyrocatechol and quercetin
equivalents, respectively. The antioxidant activity of the extracts was investigated
by using four methods including DPPH free radical and ABTS cation radical
scavenging, β-carotene bleaching and cupric reducing antioxidant capacity
(CUPRAC) assays. The methanol extract showed the highest antioxidant activity
in two assays. The anticholinesterase effect of the extracts was determined by
using Ellman method via inhibition of acetyl- (AChE) and butyryl-cholinesterase
(BChE) enzymes. The strongest inhibition against BChE was observed for the
methanol extract. The antioxidant and anticholinesterase potential of bilberry
bloom extracts was determined for the first time.

Keywords

Bilberry, Antioxidant activity, Anticholinesterase activity

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