INVOLVEMENT OF MAIN OXIDATIVE STRESS MECHANISMS IN THE TOXICITY OF BENOMYL AND CARBENDAZIM IN RATS

Year 2013, Volume: 43 Issue: 2, 103 - 120, 24.03.2014

S. Catalgol, B. Catalgol B. Alpertunga

Abstract

SUMMARY
This study aimed to evaluate the role of oxidative stress in toxicity induced
by BNL (benomyl) and its metabolite CBZ (carbendazim), which
are systemic broad-spectrum benzimidazole fungicides.
The activities of superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH-Px), glutathione reductase (GSH-Rd), γ−glutamyl
transpeptidase (GGT) and glutathione-S-transferase (GST) as well as the
levels of malonyldialdehyde (MDA), being a lipid peroxidation marker
and reduced glutathione (GSH) were measured in the liver, kidney, testis
and brain tissues of the rats, after acute exposure of the two fungicides and
their mixture.
In all tissues, it was observed that MDA levels were increased and
changes in antioxidant defense system were occurred. The induction of
oxidative stress manifested as changes in the activity and/or levels of antioxidative
parameters has been suggested as the mechanism by which BNL
and CBZ induces their toxic effects.

ÖZET
Bu çalışmada, geniş spektrumlu sistemik benzimidazol grubu fungusidlerden
olan BNL (benomyl) ve metaboliti CBZ (carbendazim)’ nin toksik
etki mekanizmalarında oksidatif stresin rolü araştırıldı. BNL, CBZ ve
karışımlarına akut maruziyet sonrası sıçanların karaciğer, böbrek, testis ve
beyin dokularında oksidatif stres göstergesi olarak lipid peroksidasyon son
ürünü malondialdehid (MDA) ve indirgenmiş glutatyon (GSH) seviyeleri,
süperoksid dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHPx),
glutatyon redüktaz (GSH-Rd), gama glutamil transpeptidaz (GGT)
ve glutatyon-S-transferaz (GST) antioksidan enzim aktivitelerinin tayini
yapıldı. Tüm dokularda MDA düzeylerinin arttığı ve antioksidan savunma
sistemlerinde değişiklik olduğu gözlendi. BNL ve CBZ’nin toksisitesinde,
antioksidan parametrelerin aktivite ve/veya düzeylerinde oksidatif stresin
indüksiyonu sonucu görülen değişikliklerin rol oynadığı sonucuna varıldı.
Key words: Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity

Keywords

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INVOLVEMENT OF MAIN OXIDATIVE STRESS MECHANISMS IN THE TOXICITY OF BENOMYL AND CARBENDAZIM IN RATS

Abstract

This study aimed to evaluate the role of oxidative stress in toxicity induced by BNL (benomyl) and its metabolite CBZ (carbendazim), which are systemic broad-spectrum benzimidazole fungicides.The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Rd), γ−glutamyl transpeptidase (GGT) and glutathione-S-transferase (GST) as well as the levels of malonyldialdehyde (MDA), being a lipid peroxidation marker and reduced glutathione (GSH) were measured in the liver, kidney, testis and brain tissues of the rats, after acute exposure of the two fungicides and their mixture.In all tissues, it was observed that MDA levels were increased and changes in antioxidant defense system were occurred. The induction of oxidative stress manifested as changes in the activity and/or levels of antioxidative parameters has been suggested as the mechanism by which BNL and CBZ induces their toxic effects. *Correspondence: [e-mail]

Keywords

Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity

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