Research Article
BibTex RIS Cite

Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines

Year 2022, , 64 - 68, 28.04.2022

Belkis Atasever Arslan , Kaan Yılancıoğlu , Seda Kuşoğlu Gültekin , İrem Gülfem Albayrak

https://doi.org/10.26650/IstanbulJPharm.2022.1057338

Abstract

Background and Aims: This study was aimed to investigate the anti-cancer effect of Isochrysis galbana microalgae extract. Methods: In the study, the chemical composition of Isochrysis galbana microalgae extract was analyzed, and its cytotoxic effect against K562, HL60, U937, MOLT-4, and Raji cancer cells was investigated. ECV304 endothelial cells were used as a healthy cell line for the understanding of its selective cytotoxicity. To determine the effects of Isochrysis galbana extract, an MTT cytotoxicity assay was performed.
Results: According to the results of the experiments, the highest cytotoxic effect of Isochrysis galbana microalgae extract was shown at about 24.07±6.48% cytotoxicity against Raji cells. There were a large number of bioactive molecules in the ex- tract, and these molecules showed a specific response to Raji cells when considering the synergistic and antagonistic effects of these molecules on each other.
Conclusion: According to the results of GC-MS analysis of Isochrysis galbana microalgae extract, the most intense molecules in the content were Dodecanoic acid, 3-hydroxy- (CAS) Beta-Hydroxy Dodecanoic Acid, and Propanoic acid, 2-methyl-, 1-(1,1-dimethylethyl)-2-methyl-1,3-isonide. The investigation of the effect of these molecules specifically against Raji cells is important to determine the possible anti-leukemic molecules and their combinations that show cytotoxicity against this cell line.

Keywords

Microalgae Cancer Cytotoxicity GC-MS

Supporting Institution

TUBITAK 3501 Career Project

Project Number

Project No: 113S251

References

  • Arslan, B.A., Isik, F.B., Gur, H., Ozen, F., & Catal, T. (2017). Apoptotic effect of Nigella sativa on human lymphoma U937 cells. Phar- macognosy Magazine, 13(3), 628-632. https://doi.org/10.4103/ PM.PM_93_17
  • Barata, J.T., Silva, A., Brandao, J.G., Nadler, L. M., Cardoso, A.A., & Boussiotis, V. A. (2004). Activation of PI3K is indispensable for interleukin 7-mediated viability, proliferation, glucose use, and growth of T cell acute lymphoblastic leukemia cells. The Journal of Experimental Medicine, 200(5), 659–669. https://doi.org/10.1084/ JEM.20040789
  • Batista, A.P., Gouveia, L., Bandarra, N.M., Franco, J.M., & Raymundo,A. (2013). Comparison of microalgal biomass profiles as novel functional ingredient for food products. Algal Research, 2(2), 164– 173. https://doi.org/10.1016/J.ALGAL.2013.01.004
  • Bechelli, J., Coppage, M., Rosell, K., & Liesveld, J. (2011). Cy- totoxicity of algae extracts on normal and malignant cells. Leukemia Research and Treatment, 2011, 1–7. https://doi. org/10.4061/2011/373519
  • Becker, E. W. (2003). Microalgae for human and animal nutrition. Amos Richmond (Eds.), Handbook of Microalgal Culture: Applied Phycology and Biotechnology(pp. 461–503).New Jersey: Blackwell Publishing Ltd. https://doi.org/10.1002/9781118567166.CH25
  • Ferdous, U.T., Norhana, Z., & Yusof, B. (2021). Algal terpenoids: A po- tential source of antioxidants for cancer therapy. Frontiers in Phar- macology, 12, 1-22.https://doi.org/10.5772/INTECHOPEN.94122
  • Folmer, F., Jaspars, M., Solano, G., Cristofanon, S., Henry, E., Tabu- dravu, J. … Diederich, M. (2009). The inhibition of TNF-alpha- induced NF-kappaB activation by marine natural products. Bio- chemical Pharmacology, 78(6), 592–606. https://doi.org/10.1016/J. BCP.2009.05.009
  • Gamal-Eldeen, A.M., Ahmed, E.F., & Abo-Zeid, M.A. (2009). In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium. Food and Chemical Toxicology : An International Journal Published for the British Indus- trial Biological Research Association, 47(6), 1378–1384. https://doi. org/10.1016/J.FCT.2009.03.016
  • Hafsa, M.B., Ismail, M.B., Garrab, M., Aly, R., Gagnon, J., & Naghmou- chi, K. (2017). Antimicrobial, antioxidant, cytotoxic and anticho- linesterase activities of water-soluble polysaccharides extracted from microalgae Isochrysis galbana and Nannochloropsis oculata. Journal of the Serbian Chemical Society, 82(5), 509–522. https://doi. org/10.2298/JSC161016036B
  • Harada, H., & Kamei, Y. (1997). Selective cytotoxicity of marine al- gae extracts to several human leukemic cell lines. Cytotechnology, 25(1–3), 213-219. https://doi.org/10.1023/A:1007987010840
  • Ho, Y.S., Yu, M.S., Lai, C.S. W., So, K.F., Yuen, W.H., & Chang, R.C.C. (2007). Characterizing the neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotox- icity. Brain Research, 1158(1), 123–134. https://doi.org/10.1016/J.BRAINRES.2007.04.075
  • Ishikawa, C., Tafuku, S., Kadekaru, T., Sawada, S., Tomita, M., Okudai- ra, T. … Mori, N. (2008). Anti-adult T-cell leukemia effects of brown algae fucoxanthin and its deacetylated product, fucoxanthinol. International Journal of Cancer, 123(11), 2702–2712. https://doi. org/10.1002/IJC.23860
  • Karawajew, L., Ruppert, V., Wuchter, C., Kösser, A., Schrappe, M., Dörken, B., & Ludwig, W.D. (2000). Inhibition of in vitro spontane- ous apoptosis by IL-7 correlates with Bcl-2 up-regulation, corti- cal/mature immunophenotype, and better early cytoreduction of childhood T-cell acute lymphoblastic leukemia. Blood, 96(1), 297–306. https://doi.org/10.1182/BLOOD.V96.1.297
  • Kaya, B., Atasever-Arslan, B., Kalkan, Z., Gür, H., & Ülküseven, B. (2016). Apoptotic mechanisms of nickel(II) complex with N1- acetylacetone-N4- 4-methoxy-salicylidene-S-allyl-thiosemicar- bazone on HL60 leukemia Cells. General Physiology and Biophysics, 35(4), 451–458. https://doi.org/10.4149/GPB_2016006A
  • Kotake-Nara, E., Terasaki, M., & Nagao, A. (2005). Characterization of apoptosis induced by fucoxanthin in human promyelocytic leukemia cells. Bioscience, Biotechnology, and Biochemistry, 69(1), 224–227. https://doi.org/10.1271/BBB.69.224
  • Kuşoğlu Gültekin, S. (2020). Deniz ürünleri kaynaklı paralitik ze- hirlenme [Paralytic poisoning from marine products]. Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 6(2), 95–99. https://dergipark.org.tr/en/pub/menba/issue/58758/795218
  • Kwon, M.J., & Nam, T.J. (2007). A polysaccharide of the marine alga Capsosiphon fulvescens induces apoptosis in AGS gastric cancer cells via an IGF-IR-mediated PI3K/Akt pathway. Cell Biology Interna- tional, 31(8), 768–775. https://doi.org/10.1016/J.CELLBI.2007.01.010
  • Machana, S., Weerapreeyakul, N., Barusrux, S., Thumanu, K., & Tan- thanuch, W. (2012). Synergistic anticancer effect of the extracts from Polyalthia evecta caused apoptosis in human hepatoma (HepG2) cells. Asian Pacific Journal of Tropical Biomedicine, 2(8), 589-596. https://doi.org/10.1016/S2221-1691(12)60103-8
  • Matos, J., Cardoso, C., Gomes, A., Campos, A.M., Falé, P., Afonso, C., & Bandarra, N.M. (2019). Bioprospection of Isochrysis galbana and its potential as a nutraceutical. Food & Function, 10(11), 7333– 7342. https://doi.org/10.1039/C9FO01364D
  • Mayer, A.M.S., & Gustafson, K.R. (2004). Marine pharmacology in 2001- 2: Antitumour and cytotoxic compounds. European Journal of Can- cer, 40(18), 2676–2704. https://doi.org/10.1016/J.EJCA.2004.09.005
  • Paul, N.A., De Nys, R., & Steinberg, P.D. (2006). Chemical defence against bacteria in the red alga Asparagopsis armata: Linking structure with function. Marine Ecology Progress Series, 306, 87– 101. https://doi.org/10.3354/MEPS306087
  • Richmond, A. (2003). The microalgal cell,. Amos Richmond (Eds.), Handbook of Microalgal Culture: Applied Phycology and Biotechnology(pp. 1-19).New Jersey: Blackwell Publishing Ltd. https://doi.org/10.1002/9780470995280
  • Sadovskaya, I., Souissi, A., Souissi, S., Grard, T., Lencel, P., Greene, C.M. … Usov, A. I. (2014). Chemical structure and biological activ- ity of a highly branched (1 → 3,1 → 6)-β-d-glucan from Isochry- sis galbana.Carbohydrate Polymers, 111, 139–148. https://doi. org/10.1016/J.CARBPOL.2014.04.077
  • Samarakoon, K., & Jeon, Y.J. (2012). Bio-functionalities of proteins derived from marine algae.. Food Research International, 48(2), 948–960. https://doi.org/10.1016/J.FOODRES.2012.03.013
  • Sánchez, S., Martínez, M.E., & Espinola, F. (2000). Biomass production and biochemical variability of the marine microalga Isochrysis gal- bana in relation to culture medium. Biochemical Engineering Jour- nal, 6(1), 13–18. https://doi.org/10.1016/S1369-703X(00)00071-1
  • Zandi, K., Tajbakhsh, S., Nabipour, I., Rastian, Z., Yousefi, F., Shara- fian, S., & Sartavi, K. (2010). In vitro antitumor activity of Gracilaria corticata (a red alga) against jurkat and molt-4 human cancer cell lines. African Journal of Biotechnology, 9(40), 6787–6790. https:// doi.org/10.5897/AJB10.602

Year 2022, , 64 - 68, 28.04.2022

Belkis Atasever Arslan , Kaan Yılancıoğlu , Seda Kuşoğlu Gültekin , İrem Gülfem Albayrak

https://doi.org/10.26650/IstanbulJPharm.2022.1057338

Abstract

Project Number

Project No: 113S251

References

  • Arslan, B.A., Isik, F.B., Gur, H., Ozen, F., & Catal, T. (2017). Apoptotic effect of Nigella sativa on human lymphoma U937 cells. Phar- macognosy Magazine, 13(3), 628-632. https://doi.org/10.4103/ PM.PM_93_17
  • Barata, J.T., Silva, A., Brandao, J.G., Nadler, L. M., Cardoso, A.A., & Boussiotis, V. A. (2004). Activation of PI3K is indispensable for interleukin 7-mediated viability, proliferation, glucose use, and growth of T cell acute lymphoblastic leukemia cells. The Journal of Experimental Medicine, 200(5), 659–669. https://doi.org/10.1084/ JEM.20040789
  • Batista, A.P., Gouveia, L., Bandarra, N.M., Franco, J.M., & Raymundo,A. (2013). Comparison of microalgal biomass profiles as novel functional ingredient for food products. Algal Research, 2(2), 164– 173. https://doi.org/10.1016/J.ALGAL.2013.01.004
  • Bechelli, J., Coppage, M., Rosell, K., & Liesveld, J. (2011). Cy- totoxicity of algae extracts on normal and malignant cells. Leukemia Research and Treatment, 2011, 1–7. https://doi. org/10.4061/2011/373519
  • Becker, E. W. (2003). Microalgae for human and animal nutrition. Amos Richmond (Eds.), Handbook of Microalgal Culture: Applied Phycology and Biotechnology(pp. 461–503).New Jersey: Blackwell Publishing Ltd. https://doi.org/10.1002/9781118567166.CH25
  • Ferdous, U.T., Norhana, Z., & Yusof, B. (2021). Algal terpenoids: A po- tential source of antioxidants for cancer therapy. Frontiers in Phar- macology, 12, 1-22.https://doi.org/10.5772/INTECHOPEN.94122
  • Folmer, F., Jaspars, M., Solano, G., Cristofanon, S., Henry, E., Tabu- dravu, J. … Diederich, M. (2009). The inhibition of TNF-alpha- induced NF-kappaB activation by marine natural products. Bio- chemical Pharmacology, 78(6), 592–606. https://doi.org/10.1016/J. BCP.2009.05.009
  • Gamal-Eldeen, A.M., Ahmed, E.F., & Abo-Zeid, M.A. (2009). In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium. Food and Chemical Toxicology : An International Journal Published for the British Indus- trial Biological Research Association, 47(6), 1378–1384. https://doi. org/10.1016/J.FCT.2009.03.016
  • Hafsa, M.B., Ismail, M.B., Garrab, M., Aly, R., Gagnon, J., & Naghmou- chi, K. (2017). Antimicrobial, antioxidant, cytotoxic and anticho- linesterase activities of water-soluble polysaccharides extracted from microalgae Isochrysis galbana and Nannochloropsis oculata. Journal of the Serbian Chemical Society, 82(5), 509–522. https://doi. org/10.2298/JSC161016036B
  • Harada, H., & Kamei, Y. (1997). Selective cytotoxicity of marine al- gae extracts to several human leukemic cell lines. Cytotechnology, 25(1–3), 213-219. https://doi.org/10.1023/A:1007987010840
  • Ho, Y.S., Yu, M.S., Lai, C.S. W., So, K.F., Yuen, W.H., & Chang, R.C.C. (2007). Characterizing the neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotox- icity. Brain Research, 1158(1), 123–134. https://doi.org/10.1016/J.BRAINRES.2007.04.075
  • Ishikawa, C., Tafuku, S., Kadekaru, T., Sawada, S., Tomita, M., Okudai- ra, T. … Mori, N. (2008). Anti-adult T-cell leukemia effects of brown algae fucoxanthin and its deacetylated product, fucoxanthinol. International Journal of Cancer, 123(11), 2702–2712. https://doi. org/10.1002/IJC.23860
  • Karawajew, L., Ruppert, V., Wuchter, C., Kösser, A., Schrappe, M., Dörken, B., & Ludwig, W.D. (2000). Inhibition of in vitro spontane- ous apoptosis by IL-7 correlates with Bcl-2 up-regulation, corti- cal/mature immunophenotype, and better early cytoreduction of childhood T-cell acute lymphoblastic leukemia. Blood, 96(1), 297–306. https://doi.org/10.1182/BLOOD.V96.1.297
  • Kaya, B., Atasever-Arslan, B., Kalkan, Z., Gür, H., & Ülküseven, B. (2016). Apoptotic mechanisms of nickel(II) complex with N1- acetylacetone-N4- 4-methoxy-salicylidene-S-allyl-thiosemicar- bazone on HL60 leukemia Cells. General Physiology and Biophysics, 35(4), 451–458. https://doi.org/10.4149/GPB_2016006A
  • Kotake-Nara, E., Terasaki, M., & Nagao, A. (2005). Characterization of apoptosis induced by fucoxanthin in human promyelocytic leukemia cells. Bioscience, Biotechnology, and Biochemistry, 69(1), 224–227. https://doi.org/10.1271/BBB.69.224
  • Kuşoğlu Gültekin, S. (2020). Deniz ürünleri kaynaklı paralitik ze- hirlenme [Paralytic poisoning from marine products]. Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 6(2), 95–99. https://dergipark.org.tr/en/pub/menba/issue/58758/795218
  • Kwon, M.J., & Nam, T.J. (2007). A polysaccharide of the marine alga Capsosiphon fulvescens induces apoptosis in AGS gastric cancer cells via an IGF-IR-mediated PI3K/Akt pathway. Cell Biology Interna- tional, 31(8), 768–775. https://doi.org/10.1016/J.CELLBI.2007.01.010
  • Machana, S., Weerapreeyakul, N., Barusrux, S., Thumanu, K., & Tan- thanuch, W. (2012). Synergistic anticancer effect of the extracts from Polyalthia evecta caused apoptosis in human hepatoma (HepG2) cells. Asian Pacific Journal of Tropical Biomedicine, 2(8), 589-596. https://doi.org/10.1016/S2221-1691(12)60103-8
  • Matos, J., Cardoso, C., Gomes, A., Campos, A.M., Falé, P., Afonso, C., & Bandarra, N.M. (2019). Bioprospection of Isochrysis galbana and its potential as a nutraceutical. Food & Function, 10(11), 7333– 7342. https://doi.org/10.1039/C9FO01364D
  • Mayer, A.M.S., & Gustafson, K.R. (2004). Marine pharmacology in 2001- 2: Antitumour and cytotoxic compounds. European Journal of Can- cer, 40(18), 2676–2704. https://doi.org/10.1016/J.EJCA.2004.09.005
  • Paul, N.A., De Nys, R., & Steinberg, P.D. (2006). Chemical defence against bacteria in the red alga Asparagopsis armata: Linking structure with function. Marine Ecology Progress Series, 306, 87– 101. https://doi.org/10.3354/MEPS306087
  • Richmond, A. (2003). The microalgal cell,. Amos Richmond (Eds.), Handbook of Microalgal Culture: Applied Phycology and Biotechnology(pp. 1-19).New Jersey: Blackwell Publishing Ltd. https://doi.org/10.1002/9780470995280
  • Sadovskaya, I., Souissi, A., Souissi, S., Grard, T., Lencel, P., Greene, C.M. … Usov, A. I. (2014). Chemical structure and biological activ- ity of a highly branched (1 → 3,1 → 6)-β-d-glucan from Isochry- sis galbana.Carbohydrate Polymers, 111, 139–148. https://doi. org/10.1016/J.CARBPOL.2014.04.077
  • Samarakoon, K., & Jeon, Y.J. (2012). Bio-functionalities of proteins derived from marine algae.. Food Research International, 48(2), 948–960. https://doi.org/10.1016/J.FOODRES.2012.03.013
  • Sánchez, S., Martínez, M.E., & Espinola, F. (2000). Biomass production and biochemical variability of the marine microalga Isochrysis gal- bana in relation to culture medium. Biochemical Engineering Jour- nal, 6(1), 13–18. https://doi.org/10.1016/S1369-703X(00)00071-1
  • Zandi, K., Tajbakhsh, S., Nabipour, I., Rastian, Z., Yousefi, F., Shara- fian, S., & Sartavi, K. (2010). In vitro antitumor activity of Gracilaria corticata (a red alga) against jurkat and molt-4 human cancer cell lines. African Journal of Biotechnology, 9(40), 6787–6790. https:// doi.org/10.5897/AJB10.602
There are 26 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Article
Authors

Belkis Atasever Arslan 0000-0001-5827-8484

Kaan Yılancıoğlu 0000-0001-7105-0898

Seda Kuşoğlu Gültekin 0000-0003-0674-1582

İrem Gülfem Albayrak 0000-0003-3218-7060

Project Number Project No: 113S251
Publication Date April 28, 2022
Submission Date January 13, 2022
Published in Issue Year 2022

Cite

APA Atasever Arslan, B., Yılancıoğlu, K., Kuşoğlu Gültekin, S., Albayrak, İ. G. (2022). Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. İstanbul Journal of Pharmacy, 52(1), 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338
AMA Atasever Arslan B, Yılancıoğlu K, Kuşoğlu Gültekin S, Albayrak İG. Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. iujp. April 2022;52(1):64-68. doi:10.26650/IstanbulJPharm.2022.1057338
Chicago Atasever Arslan, Belkis, Kaan Yılancıoğlu, Seda Kuşoğlu Gültekin, and İrem Gülfem Albayrak. “Chemical Constituent of Isochrysis Galbana Microalgae Extract and Its Cytotoxic Activities on Leukemic Cell Lines”. İstanbul Journal of Pharmacy 52, no. 1 (April 2022): 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338.
EndNote Atasever Arslan B, Yılancıoğlu K, Kuşoğlu Gültekin S, Albayrak İG (April 1, 2022) Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. İstanbul Journal of Pharmacy 52 1 64–68.
IEEE B. Atasever Arslan, K. Yılancıoğlu, S. Kuşoğlu Gültekin, and İ. G. Albayrak, “Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines”, iujp, vol. 52, no. 1, pp. 64–68, 2022, doi: 10.26650/IstanbulJPharm.2022.1057338.
ISNAD Atasever Arslan, Belkis et al. “Chemical Constituent of Isochrysis Galbana Microalgae Extract and Its Cytotoxic Activities on Leukemic Cell Lines”. İstanbul Journal of Pharmacy 52/1 (April 2022), 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338.
JAMA Atasever Arslan B, Yılancıoğlu K, Kuşoğlu Gültekin S, Albayrak İG. Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. iujp. 2022;52:64–68.
MLA Atasever Arslan, Belkis et al. “Chemical Constituent of Isochrysis Galbana Microalgae Extract and Its Cytotoxic Activities on Leukemic Cell Lines”. İstanbul Journal of Pharmacy, vol. 52, no. 1, 2022, pp. 64-68, doi:10.26650/IstanbulJPharm.2022.1057338.
Vancouver Atasever Arslan B, Yılancıoğlu K, Kuşoğlu Gültekin S, Albayrak İG. Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. iujp. 2022;52(1):64-8.