Research Article
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Effect of Melaleuca alternifolia oil on cytotoxicity and neuropeptide y gene expression

Year 2023, , 58 - 62, 28.04.2023

İrem Gülfem Albayrak , Seda Kuşoğlu Gültekin , Muhsin Konuk

https://doi.org/10.26650/IstanbulJPharm.2023.1187329

Abstract

Background and Aims: This study aims to investigate the effect of tea tree oil (TTO), which is an oil produced by steam distil- lation of the Melaleuca alternifolia (Maiden & Betche) Cheel plant terminal branches and leaves, on changes in neuropeptide Y (NPY) gene expression in SH-SY5Y neuroblastoma cells.
Methods: The first stage of this study investigates the cytotoxic/proliferative effects of TTO solutions prepared at different concentrations on the SH-SY5Y cell line using the tetrazolium reduction (MTT) assay. The next stage analyzes the effects of the determined TTO concentrations on NPY gene expression using the real-time polymerase chain reaction (qPCR) method. Results: TTO concentrations prepared at a ratio of 1:32, 1:64, and 1:28 (v/v) showed statistically significant effects on cells in the cytotoxicity test and were used in the gene expression analysis. The highest significant gene expression change was seen in the cells in which the TTO solution had been applied at a ratio of 1:64 for 24 hours. NPY gene expression in these cells was determined to have increased 2.24 times compared to control cells.
Conclusion: Upon evaluating the results from the MTT assay and gene expression analysis, the solutions of the cells prepared at different TTO ratios were determined to have caused changes in gene expression. Future studies will be able to reveal all affected molecular pathways by increasing research involving TTO.

Keywords

Gene expression neuroblastoma NPY SH-SY5Y tea tree oil

Supporting Institution

This study was supported by the Üsküdar University Scientific Research Projects Department.

Project Number

MDBF-20-02

References

  • Álvaro, A.R., Rosmaninho-Salgado, J., Santiago, A.R., Martins, J., Aveleira, C., Santos, P.F., Pereira, T., Gouveia, D., Carvalho, A.L., Grouzmann, E., Ambrósio, A.F., Cavadas, C. (2007). NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells. Neurochemistry International, 50(5), 757–763. https://doi.org/10.1016/J.NEUINT.2007.01.010
  • Arcella, A., & Sanchez, M. (2021). Natural substances to potentiate canonical glioblastoma chemotherapy. Journal of Chemotherapy, 33(5), 276-287. https://doi.org/10.1080/1120009X.2021.1873633
  • Atkinson, N., & Brice, H.E. (1955). Antibacterial substances pro- duced by flowering plants. II. The antibacterial action of essential oils from some Australian plants. The Australian Journal of Experi- mental Biology and Medical Science, 33(5), 547–554. https://doi. org/10.1038/icb.1955.56
  • Bao, Y., Wang, W., Zhou, Z., & Sun, C. (2014). Benefits and risks of the hormetic effects of dietary isothiocyanates on cancer pre- vention. PLoS ONE, 9(12), 114764. https://doi.org/10.1371/journal. pone.0114764
  • Barnea, A., Roberts, J., Keller, P., & Word, R.A. (2001). Interleukin- 1β induces expression of neuropeptide Y in primary astrocyte cultures in a cytokine-specific manner: induction in human but not rat astrocytes. Brain Research, 896(1–2), 137–145. https://doi. org/10.1016/S0006-8993(01)02141-2
  • Carson, C.F., Hammer, K.A., & Riley, T.V. (2006). Melaleuca alterni- folia (tea tree) oil: A review of antimicrobial and other medicinal properties. Clinical Microbiology Reviews, 19, 50–62. https://doi. org/10.1128/CMR.19.1.50-62.2006
  • Duarte-Neves, J., Pereira de Almeida, L., & Cavadas, C. (2016). Neuropeptide Y (NPY) as a therapeutic target for neurodegen- erative diseases. Neurobiology of Disease, 95, 210–224. https://doi. org/10.1016/J.NBD.2016.07.022
  • Farrelly, L.A., Savage, N.T.P., O’Callaghan, C., Toulouse, A., & Yilma- zer-Hanke, D.M. (2013). Therapeutic concentrations of valproate but not amitriptyline increase neuropeptide Y (NPY) expression in the human SH-SY5Y neuroblastoma cell line. Regulatory Pep- tides, 186, 123–130. https://doi.org/10.1016/j.regpep.2013.08.005
  • Fendt, M., & Fanselow, M.S. (1999). The neuroanatomical and neurochemical basis of conditioned fear. Neuroscience & Biobe- havioral Reviews, 23(5), 743–760. https://doi.org/10.1016/S0149-7634(99)00016-0
  • Ferreira, R., Xapelli, S., Santos, T., Silva, A.P., Cristóvão, A., Cortes, L., & Malva, J.O. (2010). Neuropeptide Y Modulation of Interleukin- 1β (IL-1β)-induced Nitric Oxide Production in Microglia. Jour- nal of Biological Chemistry, 285(53), 41921–41934. https://doi. org/10.1074/JBC.M110.164020
  • Hayes, D.P. (2007). Nutritional hormesis. European Journal of Clini- cal Nutrition, 61, 147–159. https://doi.org/10.1038/sj.ejcn.1602507
  • Heilig, M. (2004). The NPY system in stress, anxiety and depres- sion. Neuropeptides, 38(4), 213–224. https://doi.org/10.1016/J. NPEP.2004.05.002
  • Heilig, M., & Murison, R. (1987). Intracerebroventricular neuropep- tide Y protects against stress-induced gastric erosion in the rat. European Journal of Pharmacology, 137(1), 127–129. https://doi. org/10.1016/0014-2999(87)90191-9
  • Inouye, S., Tsuruoka, T., Watanabe, M., Takeo, K., Akao, M., Nishiya- ma, Y., & Yamaguchi, H. (2000). Inhibitory effect of essential oils on apical growth of Aspergillus fumigatus by vapour contact. Mycoses, 43(1–2), 17–23. https://doi.org/10.1046/j.1439-0507.2000.00538.x
  • Inouye, S., Uchida, K., & Yamaguchi, H. (2001). In-vitro and in- vivo anti-Trichophyton activity of essential oils by vapour contact. Mycoses, 44(3–4), 99–107. https://doi.org/10.1046/j.1439- 0507.2001.00618.x
  • Inouye, Watanabe, M., Nishiyama, Y., Takco, K., Akao, M., & Ya- maguchi, H. (1998). Antisporulating and respiration-inhibitory effects of essential oils on filamentous fungi. Mycoses, 41(9–10), 403–410. https://doi.org/10.1111/j.1439-0507.1998.tb00361.x
  • Kaya, B., Atasever-Arslan, B., Kalkan, Z., Gür, H., & Ülküseven, B. (2016). Apoptotic mechanisms of nickel(II) complex with N1acet- ylacetone-N4- 4-methoxy-salicylidene-S-allyl-thiosemicarbazone on HL60 leukemia cells. General Physiology and Biophysics, 35(4), 451–458. https://doi.org/10.4149/GPB_2016006A
  • Khan, S.A., McLean, M. K., & Slater, M.R. (2013). Concentrated tea tree oil toxicosis in dogs and cats: 443 cases (2002-2012). Jour- nal of the American Veterinary Medical Association, 244(1), 95–99. https://doi.org/10.2460/javma.244.1.95
  • Kitlinska, J. (2007). Neuropeptide Y (NPY) in neuroblastoma: Effect on growth and vascularization. Peptides, 28(2), 405–412. https:// doi.org/10.1016/J.PEPTIDES.2006.08.038
  • Kumari, P. (2013). Antimicrobial properties of tea tree oil. Interna- tional Journal of Bioinformatics and Biological Science, 1(1), 71–77.
  • Low, D., Rawal, B.D., & Griffin, W.J. (1974). Antibacterial action of the essential oils of some Australian myrtaceae with special references to the activity of chromatographic fractions of oil of Eucalyptus citriodora. Planta Medica, 26(2), 184–189. https://doi. org/10.1055/s-0028-1097987
  • Morris, M.C., Donoghue, A., Markowitz, J.A., & Osterhoudt, K.C. (2003). Ingestion of tea tree oil (Melaleuca oil) by a 4-year- old boy. Pediatric Emergency Care, 19(3), 169–171. https://doi. org/10.1097/01.pec.0000081241.98249.7b
  • Naveilhan, P., Canals, J.M., Valjakka, A., Vartiainen, J., Arenas, E., & Ernfors, P. (2001). Neuropeptide Y alters sedation through a hypothalamic Y1-mediated mechanism. European Journal of Neuroscience, 13(12), 2241–2246. https://doi.org/10.1046/j.0953- 816X.2001.01601.x
  • Redrobe, J. P., Dumont, Y., Fournier, A., & Quirion, R. (2002). The neuropeptide Y (NPY) Y1 receptor subtype mediates NPY-in- duced antidepressant-like activity in the mouse forced swim- ming test. Neuropsychopharmacology, 26(5), 615–624. https://doi. org/10.1016/S0893-133X(01)00403-1
  • Sajdyk, T.J., Vandergriff, M.G., & Gehlert, D.R. (1999). Amygdalar neuropeptide Y Y1 receptors mediate the anxiolytic-like actions of neuropeptide Y in the social interaction test. European Jour- nal of Pharmacology, 368(2–3), 143–147. https://doi.org/10.1016/ S0014-2999(99)00018-7
  • Silva, A.P., Cavadas, C., & Grouzmann, E. (2002). Neuropeptide Y and its receptors as potential therapeutic drug targets. Clinica Chimica Acta; International Journal of Clinical Chemistry, 326(1–2), 3–25. https://doi.org/10.1016/S0009-8981(02)00301-7
  • Stogner, K.A., & Holmes, P.V. (2000). Neuropeptide-Y exerts anti- depressant-like effects in the forced swim test in rats. European Journal of Pharmacology, 387(2), R9–R10. https://doi.org/10.1016/ S0014-2999(99)00800-6
  • Villar, D., Knight, M.J., Hansen, S.R., & Buck, W.B. (1994, April 1). Tox- icity of melaleuca oil and related essential oils applied topically on dogs and cats. Veterinary and Human Toxicology, 36, 139–142. Retrieved from https://europepmc.org/article/med/8197716
  • Wang, D., Calabrese, E.J., Lian, B., Lin, Z., & Calabrese, V. (2018, April 1). Hormesis as a mechanistic approach to understanding herbal treatments in traditional Chinese medicine. Pharmacol- ogy and Therapeutics, 184, 42–50. https://doi.org/10.1016/j.phar- mthera.2017.10.013

Year 2023, , 58 - 62, 28.04.2023

İrem Gülfem Albayrak , Seda Kuşoğlu Gültekin , Muhsin Konuk

https://doi.org/10.26650/IstanbulJPharm.2023.1187329

Abstract

Project Number

MDBF-20-02

References

  • Álvaro, A.R., Rosmaninho-Salgado, J., Santiago, A.R., Martins, J., Aveleira, C., Santos, P.F., Pereira, T., Gouveia, D., Carvalho, A.L., Grouzmann, E., Ambrósio, A.F., Cavadas, C. (2007). NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells. Neurochemistry International, 50(5), 757–763. https://doi.org/10.1016/J.NEUINT.2007.01.010
  • Arcella, A., & Sanchez, M. (2021). Natural substances to potentiate canonical glioblastoma chemotherapy. Journal of Chemotherapy, 33(5), 276-287. https://doi.org/10.1080/1120009X.2021.1873633
  • Atkinson, N., & Brice, H.E. (1955). Antibacterial substances pro- duced by flowering plants. II. The antibacterial action of essential oils from some Australian plants. The Australian Journal of Experi- mental Biology and Medical Science, 33(5), 547–554. https://doi. org/10.1038/icb.1955.56
  • Bao, Y., Wang, W., Zhou, Z., & Sun, C. (2014). Benefits and risks of the hormetic effects of dietary isothiocyanates on cancer pre- vention. PLoS ONE, 9(12), 114764. https://doi.org/10.1371/journal. pone.0114764
  • Barnea, A., Roberts, J., Keller, P., & Word, R.A. (2001). Interleukin- 1β induces expression of neuropeptide Y in primary astrocyte cultures in a cytokine-specific manner: induction in human but not rat astrocytes. Brain Research, 896(1–2), 137–145. https://doi. org/10.1016/S0006-8993(01)02141-2
  • Carson, C.F., Hammer, K.A., & Riley, T.V. (2006). Melaleuca alterni- folia (tea tree) oil: A review of antimicrobial and other medicinal properties. Clinical Microbiology Reviews, 19, 50–62. https://doi. org/10.1128/CMR.19.1.50-62.2006
  • Duarte-Neves, J., Pereira de Almeida, L., & Cavadas, C. (2016). Neuropeptide Y (NPY) as a therapeutic target for neurodegen- erative diseases. Neurobiology of Disease, 95, 210–224. https://doi. org/10.1016/J.NBD.2016.07.022
  • Farrelly, L.A., Savage, N.T.P., O’Callaghan, C., Toulouse, A., & Yilma- zer-Hanke, D.M. (2013). Therapeutic concentrations of valproate but not amitriptyline increase neuropeptide Y (NPY) expression in the human SH-SY5Y neuroblastoma cell line. Regulatory Pep- tides, 186, 123–130. https://doi.org/10.1016/j.regpep.2013.08.005
  • Fendt, M., & Fanselow, M.S. (1999). The neuroanatomical and neurochemical basis of conditioned fear. Neuroscience & Biobe- havioral Reviews, 23(5), 743–760. https://doi.org/10.1016/S0149-7634(99)00016-0
  • Ferreira, R., Xapelli, S., Santos, T., Silva, A.P., Cristóvão, A., Cortes, L., & Malva, J.O. (2010). Neuropeptide Y Modulation of Interleukin- 1β (IL-1β)-induced Nitric Oxide Production in Microglia. Jour- nal of Biological Chemistry, 285(53), 41921–41934. https://doi. org/10.1074/JBC.M110.164020
  • Hayes, D.P. (2007). Nutritional hormesis. European Journal of Clini- cal Nutrition, 61, 147–159. https://doi.org/10.1038/sj.ejcn.1602507
  • Heilig, M. (2004). The NPY system in stress, anxiety and depres- sion. Neuropeptides, 38(4), 213–224. https://doi.org/10.1016/J. NPEP.2004.05.002
  • Heilig, M., & Murison, R. (1987). Intracerebroventricular neuropep- tide Y protects against stress-induced gastric erosion in the rat. European Journal of Pharmacology, 137(1), 127–129. https://doi. org/10.1016/0014-2999(87)90191-9
  • Inouye, S., Tsuruoka, T., Watanabe, M., Takeo, K., Akao, M., Nishiya- ma, Y., & Yamaguchi, H. (2000). Inhibitory effect of essential oils on apical growth of Aspergillus fumigatus by vapour contact. Mycoses, 43(1–2), 17–23. https://doi.org/10.1046/j.1439-0507.2000.00538.x
  • Inouye, S., Uchida, K., & Yamaguchi, H. (2001). In-vitro and in- vivo anti-Trichophyton activity of essential oils by vapour contact. Mycoses, 44(3–4), 99–107. https://doi.org/10.1046/j.1439- 0507.2001.00618.x
  • Inouye, Watanabe, M., Nishiyama, Y., Takco, K., Akao, M., & Ya- maguchi, H. (1998). Antisporulating and respiration-inhibitory effects of essential oils on filamentous fungi. Mycoses, 41(9–10), 403–410. https://doi.org/10.1111/j.1439-0507.1998.tb00361.x
  • Kaya, B., Atasever-Arslan, B., Kalkan, Z., Gür, H., & Ülküseven, B. (2016). Apoptotic mechanisms of nickel(II) complex with N1acet- ylacetone-N4- 4-methoxy-salicylidene-S-allyl-thiosemicarbazone on HL60 leukemia cells. General Physiology and Biophysics, 35(4), 451–458. https://doi.org/10.4149/GPB_2016006A
  • Khan, S.A., McLean, M. K., & Slater, M.R. (2013). Concentrated tea tree oil toxicosis in dogs and cats: 443 cases (2002-2012). Jour- nal of the American Veterinary Medical Association, 244(1), 95–99. https://doi.org/10.2460/javma.244.1.95
  • Kitlinska, J. (2007). Neuropeptide Y (NPY) in neuroblastoma: Effect on growth and vascularization. Peptides, 28(2), 405–412. https:// doi.org/10.1016/J.PEPTIDES.2006.08.038
  • Kumari, P. (2013). Antimicrobial properties of tea tree oil. Interna- tional Journal of Bioinformatics and Biological Science, 1(1), 71–77.
  • Low, D., Rawal, B.D., & Griffin, W.J. (1974). Antibacterial action of the essential oils of some Australian myrtaceae with special references to the activity of chromatographic fractions of oil of Eucalyptus citriodora. Planta Medica, 26(2), 184–189. https://doi. org/10.1055/s-0028-1097987
  • Morris, M.C., Donoghue, A., Markowitz, J.A., & Osterhoudt, K.C. (2003). Ingestion of tea tree oil (Melaleuca oil) by a 4-year- old boy. Pediatric Emergency Care, 19(3), 169–171. https://doi. org/10.1097/01.pec.0000081241.98249.7b
  • Naveilhan, P., Canals, J.M., Valjakka, A., Vartiainen, J., Arenas, E., & Ernfors, P. (2001). Neuropeptide Y alters sedation through a hypothalamic Y1-mediated mechanism. European Journal of Neuroscience, 13(12), 2241–2246. https://doi.org/10.1046/j.0953- 816X.2001.01601.x
  • Redrobe, J. P., Dumont, Y., Fournier, A., & Quirion, R. (2002). The neuropeptide Y (NPY) Y1 receptor subtype mediates NPY-in- duced antidepressant-like activity in the mouse forced swim- ming test. Neuropsychopharmacology, 26(5), 615–624. https://doi. org/10.1016/S0893-133X(01)00403-1
  • Sajdyk, T.J., Vandergriff, M.G., & Gehlert, D.R. (1999). Amygdalar neuropeptide Y Y1 receptors mediate the anxiolytic-like actions of neuropeptide Y in the social interaction test. European Jour- nal of Pharmacology, 368(2–3), 143–147. https://doi.org/10.1016/ S0014-2999(99)00018-7
  • Silva, A.P., Cavadas, C., & Grouzmann, E. (2002). Neuropeptide Y and its receptors as potential therapeutic drug targets. Clinica Chimica Acta; International Journal of Clinical Chemistry, 326(1–2), 3–25. https://doi.org/10.1016/S0009-8981(02)00301-7
  • Stogner, K.A., & Holmes, P.V. (2000). Neuropeptide-Y exerts anti- depressant-like effects in the forced swim test in rats. European Journal of Pharmacology, 387(2), R9–R10. https://doi.org/10.1016/ S0014-2999(99)00800-6
  • Villar, D., Knight, M.J., Hansen, S.R., & Buck, W.B. (1994, April 1). Tox- icity of melaleuca oil and related essential oils applied topically on dogs and cats. Veterinary and Human Toxicology, 36, 139–142. Retrieved from https://europepmc.org/article/med/8197716
  • Wang, D., Calabrese, E.J., Lian, B., Lin, Z., & Calabrese, V. (2018, April 1). Hormesis as a mechanistic approach to understanding herbal treatments in traditional Chinese medicine. Pharmacol- ogy and Therapeutics, 184, 42–50. https://doi.org/10.1016/j.phar- mthera.2017.10.013
There are 29 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Original Article
Authors

İrem Gülfem Albayrak 0000-0003-3218-7060

Seda Kuşoğlu Gültekin 0000-0003-0674-1582

Muhsin Konuk 0000-0002-6651-718X

Project Number MDBF-20-02
Publication Date April 28, 2023
Submission Date October 11, 2022
Published in Issue Year 2023

Cite

APA Albayrak, İ. G., Kuşoğlu Gültekin, S., & Konuk, M. (2023). Effect of Melaleuca alternifolia oil on cytotoxicity and neuropeptide y gene expression. İstanbul Journal of Pharmacy, 53(1), 58-62. https://doi.org/10.26650/IstanbulJPharm.2023.1187329
AMA Albayrak İG, Kuşoğlu Gültekin S, Konuk M. Effect of Melaleuca alternifolia oil on cytotoxicity and neuropeptide y gene expression. iujp. April 2023;53(1):58-62. doi:10.26650/IstanbulJPharm.2023.1187329
Chicago Albayrak, İrem Gülfem, Seda Kuşoğlu Gültekin, and Muhsin Konuk. “Effect of Melaleuca Alternifolia Oil on Cytotoxicity and Neuropeptide Y Gene Expression”. İstanbul Journal of Pharmacy 53, no. 1 (April 2023): 58-62. https://doi.org/10.26650/IstanbulJPharm.2023.1187329.
EndNote Albayrak İG, Kuşoğlu Gültekin S, Konuk M (April 1, 2023) Effect of Melaleuca alternifolia oil on cytotoxicity and neuropeptide y gene expression. İstanbul Journal of Pharmacy 53 1 58–62.
IEEE İ. G. Albayrak, S. Kuşoğlu Gültekin, and M. Konuk, “Effect of Melaleuca alternifolia oil on cytotoxicity and neuropeptide y gene expression”, iujp, vol. 53, no. 1, pp. 58–62, 2023, doi: 10.26650/IstanbulJPharm.2023.1187329.
ISNAD Albayrak, İrem Gülfem et al. “Effect of Melaleuca Alternifolia Oil on Cytotoxicity and Neuropeptide Y Gene Expression”. İstanbul Journal of Pharmacy 53/1 (April 2023), 58-62. https://doi.org/10.26650/IstanbulJPharm.2023.1187329.
JAMA Albayrak İG, Kuşoğlu Gültekin S, Konuk M. Effect of Melaleuca alternifolia oil on cytotoxicity and neuropeptide y gene expression. iujp. 2023;53:58–62.
MLA Albayrak, İrem Gülfem et al. “Effect of Melaleuca Alternifolia Oil on Cytotoxicity and Neuropeptide Y Gene Expression”. İstanbul Journal of Pharmacy, vol. 53, no. 1, 2023, pp. 58-62, doi:10.26650/IstanbulJPharm.2023.1187329.
Vancouver Albayrak İG, Kuşoğlu Gültekin S, Konuk M. Effect of Melaleuca alternifolia oil on cytotoxicity and neuropeptide y gene expression. iujp. 2023;53(1):58-62.