Double-Layer Nanofibrous Mats Loaded with Lidocaine Hydrochloride and Silver Sulfadiazine: Physicochemical Characterization and in vitro Release Studies

Year 2025, Volume: 55 Issue: 3, 366 - 377, 14.01.2026

Sewar Talal Ali Abuassi , Emine Kahraman

https://doi.org/10.26650/IstanbulJPharm.2025.1691720

https://izlik.org/JA73HF49FU

Abstract

Background and Aims: Electrospun nanofibrous systems are promising platforms for wound healing due to their high surface area, porosity, and tunable drug release. This study aimed to develop a doublelayered electrospun nanofibrous mat incorporating lidocaine hydrochloride and silver sulfadiazine for potential burn and wound treatment, focusing on the physicochemical characteristics and in vitro drug release.

Methods: Polyvinyl alcohol and polyurethane served as polymer matrices for loading lidocaine hydrochloride and silver sulfadiazine, respectively. The electrospinning conditions were optimised varying polymer concentrations (5 – 16% w/v), voltage, flow rate, and tip-to-collector distance. The resulting mats were characterised for drug–polymer compatibility, morphology, fibre diameter, porosity, tensile strength, drug content uniformity, and in vitro drug release. The drug release data were fitted to zero-order, first-order, Higuchi, and Korsmeyer–Peppas models.

Results: Smooth, bead-free nanofibers forming the double-layer mat were successfully fabricated using optimised electrospinning solutions and process parameters. FT-IR and DSC analysis confirmed no significant drug–polymer interactions. SEM images and porosity analysis demonstrated that the doublelayer structure possessed high surface area and porosity, which are favourable for wound treatment. Furthermore, the mat exhibited adequate tensile strength and uniform drug distribution. in vitro drug release results indicated that PVA layer provided rapid release (100% within 90 min), supporting the desired anaesthetic effect. In contrast, PU layer ensured the sustained release of the antimicrobial drug, following the Korsmeyer–Peppas kinetics indicative of the diffusion-controlled mechanism.

Conclusion: These findings highlight the potential of double-layer nanofibrous mat as a promising approach for burn and wound care, combining an early anaesthetic effect with sustained antimicrobial action.

Keywords

Burn , Electrospinning , Lidocaine hydrochloride , Nanofiber , Silver sulfadiazine , Wound

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