Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD
Abstract
DOI:
10.5152/IstanbulJPharm.2018.416835
In this study, a reversed-phase, high pressure liquid chromatography (RP-HPLC) method was applied in the quantitative analysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn. var. alpinus collected from Rize during the flowering and fruiting seasons. A simple method to extract lycorine from the plant specimens was performed, using pre-packed columns with diatomaceous earth (Extrelut®). The chromatographic separation was achieved employing an isocratic system with a mobile phase including trifluoroacetic acid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using a diode array detector (DAD). The lycorine content in the bulbs during the flowering and fruiting seasons was detected as 0.01576 and 0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise.
Keywords
References
- Reference 1: Derya Çiçek Polat---polatd@ankara.edu.trReference 2: Fatma Tosun---ftosun@medipol.edu.tr
Details
Primary Language
English
Subjects
Pharmacology and Pharmaceutical Sciences
Journal Section
Research Article
Publication Date
August 31, 2018
Submission Date
April 19, 2018
Acceptance Date
July 19, 2018
Published in Issue
Year 2018 Volume: 48 Number: 2