Abstract
References
- Reference 1: Derya Çiçek Polat---polatd@ankara.edu.trReference 2: Fatma Tosun---ftosun@medipol.edu.tr
Abstract
DOI:
10.5152/IstanbulJPharm.2018.416835
In this study, a reversed-phase, high
pressure liquid chromatography (RP-HPLC) method was applied in the quantitative
analysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn.
var. alpinus collected from Rize during the flowering and fruiting seasons. A
simple method to extract lycorine from the
plant specimens was performed, using pre-packed columns with
diatomaceous earth (Extrelut®). The chromatographic separation was achieved
employing an isocratic system with a mobile phase including trifluoroacetic
acid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using a
diode array detector (DAD). The lycorine content in the bulbs during the
flowering and fruiting seasons was detected as 0.01576 and 0.02351 %,
respectively. Also, validation studies showed that the method was specific,
accurate and precise.
References
- Reference 1: Derya Çiçek Polat---polatd@ankara.edu.trReference 2: Fatma Tosun---ftosun@medipol.edu.tr
Details
| Primary Language | English |
|---|---|
| Subjects | Pharmacology and Pharmaceutical Sciences |
| Journal Section | Research Article |
| Authors | |
| Submission Date | April 19, 2018 |
| Publication Date | August 31, 2018 |
| Published in Issue | Year 2018 Volume: 48 Issue: 2 |