Enhancing the antibacterial activity of the biosynthesized silver nanoparticles by “püse”

Abstract

Background and Aims: The silver nanoparticles (Ag NPs) synthesized by green synthesis have antimicrobial properties and their potential to be used in the medical field is quite high. Püse, also called “black doctor”, is widely used as a treatment for the health problems of animals and in folk medicine in Turkish nomad culture. It has been reported that Ag NPs biosynthesized with Juglans regia leaf extracts have antibacterial activity. The aim and importance of this study is the preparation of a mixture of "püse" and Ag NPs for the first time and the investigation of its antibacterial activity. Methods: In this paper, Ag NPs were synthesized by using J. regia leaves and morphological characteristics of the NPs obtained were determined using TEM. The formation of NPs was identified using an UV-Vis spectrophotometer. The antibacterial activity was investigated by combining the obtained and characterized Ag NPs with the "püse". The antibacterial activity of the mixture was tested on Gram (+) bacteria and Gram (-) bacter. Results: The mixture that was prepared with püse and Ag NPs showed higher antibacterial activity than antibiotics. Conclusion: The positive results showed that this mixture has the potential to be used as a preparation especially in the treatment of animal wounds and the skin infections caused by bacteria.

Keywords

• Biosynthesized
• silver nanoparticles
• antibacterial activity
• Juglans regia
• püse

References

1. • Ak, M. (2017). Folk Medıcıne In The Yoruks. International Journal of Social Science, 57, 395–405.
2. • Alam, F., Islam, M. A., Kamal, M. A., & Gan, S. H. (2018). Updates on managing type 2 diabetes mellitus with natural products: towards antidiabetic drug development. Current Medicinal Chemistry, 25(39), 5395–5431.
3. • Alptekin, M. (2019). Mersin Folklorunda Püse (Kara Hekim) İle İlgili Tespitler. Karabük Üniversitesi Sosyal Bilimler Enstitüsü Dergisi, 9(1), 323–336.
4. • Al-Sheddi, E. S., Farshori, N. N., Al-Oqail, M. M., Al-Massarani, S. M., Saquib, Q., Wahab, R., ... Siddiqui, M. A. (2018). Anticancer potential of green synthesized silver nanoparticles using extract of Nepeta deflersiana against human cervical cancer cells (HeLA). Bioinorganic Chemistry and Applications, 2018, 9390784.
5. • Cameron, S. J., Hosseinian, F., & Willmore, W. G. (2018). A current overview of the biological and cellular effects of nanosilver. International Journal of Molecular Sciences, 19(7), 2030.
6. • Chaloupka, K., Malam, Y., & Seifalian, A. M. (2010). Nanosilver as a new generation of nanoproduct in biomedical applications. Trends In Biotechnology, 28(11), 580–588.
7. • Collins, A. S. (2008). Preventing Health Care–Associated Infections. In R. G. Hughes (Ed.), Patient Safety and Quality: An Evidence- Based Handbook for Nurses. Agency for Healthcare Research and Quality (US).
8. • de la Parra, J., & Quave, C. L. (2017). Ethnophytotechnology: Harnessing the power of ethnobotany with biotechnology. Trends In Biotechnology, 35(9), 802–806.

9. • Demirbas, A., Welt, B. A., & Ocsoy, I. (2016). Biosynthesis of red cabbage extract directed Ag NPs and their effect on the loss of antioxidant activity. Materials Letters, 179, 20–23. https://doi. org/10.1016/j.matlet.2016.05.056
10. • Dolatabadi, S., Moghadam, H. N., & Mahdavi-Ourtakand, M. (2018). Evaluating the anti-biofilm and antibacterial effects of Juglans regia L. extracts against clinical isolates of Pseudomonas aeruginosa. Microbial Pathogenesis, 118, 285–289.
11. • Duman, F., Ocsoy, I., & Kup, F. O. (2016). Chamomile flower extractdirected CuO nanoparticle formation for its antioxidant and DNA cleavage properties. Materials Science and Engineering: C, 60, 333–338.
12. • Faraz, N., Islam, Z., & Rehman, R. (2012). Sehrish Antibiofilm forming activity of naturally occurring compound. Biomedica, 28(2), 171–175.
13. • Forough, M., & Fahadı, K. (2010). Biological and green synthesis of silver nanoparticles. Turkish Journal of Engineering and Environmental Sciences, 34(4), 281–287.
14. • Garnatje, T., Peñuelas, J., & Vallès, J. (2017). Ethnobotany, phylogeny, and 'omics' for human health and food security. Trends In Plant Science, 22(3), 187–191.
15. • Gurib-Fakim, A. (2006). Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine, 27(1), 1–93.
16. • Haque, M., Sartelli, M., McKimm, J., & Bakar, M. A. (2018). Health care-associated infections–an overview. Infection and Drug Resistance, 11, 2321–2333.
17. • Hidron, A. I., Edwards, J. R., Patel, J., Horan, T. C., Sievert, D. M., Pollock, D. A., & Fridkin, S. K. (2008). Antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006– 2007. Infection Control & Hospital Epidemiology, 29(11), 996–1011.
18. • Irobi, O. N., Moo-Young, M., Anderson, W. A., & Daramola, S. O. (1994). Antimicrobial activity of bark extracts of Bridelia ferruginea (Euphorbiaceae). Journal of Ethnopharmacology, 43(3), 185–190.
19. • Karatoprak, G. Ş., Aydin, G., Altinsoy, B., Altinkaynak, C., Koşar, M., & Ocsoy, I. (2017). The Effect of Pelargonium endlicherianum Fenzl. root extracts on formation of nanoparticles and their antimicrobial activities. Enzyme and Microbial Technology, 97, 21–26.
20. • Kaur, K., Michael, H., Arora, S., Harkonen, P. L., & Kumar, S. (2003). Studies on correlation of antimutagenic and antiproliferative activities of Juglans regia L. Journal of environmental pathology, Toxicology and Oncology, 22(1), 59–67.
21. • Kızılarslan, Ç., & Sevgi, E. (2013). Ethnobotanical uses of genus Pinus L.(Pinaceae) in Turkey. Indian Journal of Traditional Knowledge, 12, 209–220.
22. • Luther, E. M., Schmidt, M. M., Diendorf, J., Epple, M., & Dringen, R. (2012). Upregulation of metallothioneins after exposure of cultured primary astrocytes to silver nanoparticles. Neurochemical Research, 37(8), 1639–1648.
23. • Magill, S. S., Edwards, J. R., Bamberg, W., Beldavs, Z. G., Dumyati, G., Kainer, M. A., ... Ray, S. M. (2014). Multistate point-prevalence survey of health care–associated infections. New England Journal of Medicine, 370(13), 1198–1208.
24. • McShan, D., Ray, P. C., & Yu, H. (2014). Molecular toxicity mechanism of nanosilver. Journal of Food and Drug Analysis, 22(1), 116– 127.
25. • Moravej, H., Salehi, A., Razavi, Z., Moein, M. R., Etemadfard, H., Karami, F., & Ghahremani, F. (2016). Chemical composition and the effect of walnut hydrosol on glycemic control of patients with type 1 diabetes. International Journal of Endocrinology and Metabolism, 14(1).
26. • Mohammadi, J., Mirzaie, A., Azizi, A., Roozbehi, A., & Delaviz, H. (2012). The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model. ISMJ, 15(4), 293–302.
27. • Munita, J. M., & Arias, C. A. (2016). Mechanisms of antibiotic resistance. Virulence Mechanisms of Bacterial Pathogens, 4(2), 481–511.
28. • Nahrstedt, A., Vetter, U., & Hammerschmidt, F. J. (1981). Composition of the steam distillation product from the leaves of juglans regia (author’s transl). Planta Medica, 42(4), 313.
29. • Ocsoy, I., Gulbakan, B., Chen, T., Zhu, G., Chen, Z., Sari, M. M., ... Tan, W. (2013). DNA‐guided metal‐nanoparticle formation on graphene oxide surface. Advanced Materials, 25(16), 2319–2325.
30. • Ocsoy, I., Temiz, M., Celik, C., Altinsoy, B., Yilmaz, V., & Duman, F. (2017). A green approach for formation of silver nanoparticles on magnetic graphene oxide and highly effective antimicrobial activity and reusability. Journal of Molecular Liquids, 227, 147–152.
31. • Okaiyeto, K., Ojemaye, M. O., Hoppe, H., Mabinya, L. V., & Okoh, A. I. (2019). Phytofabrication of Silver/Silver Chloride Nanoparticles Using Aqueous Leaf Extract of Oedera genistifolia: Characterization and Antibacterial Potential. Molecules, 24(23), 4382.
32. • Paudel, P., Satyal, P., Dosoky, N. S., Maharjan, S., & Setzer, W. N. (2013). Juglans regia and J. nigra, two trees important in traditional medicine: A comparison of leaf essential oil compositions and biological activities. Natural Product Communications, 8(10), 1934578X1300801038.
33. • Pereira, J. A., Oliveira, I., Sousa, A., Valentão, P., Andrade, P. B., Ferreira, I. C., ... Estevinho, L. (2007). Walnut (Juglans regia L.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food and Chemical Toxicology, 45(11), 2287–2295.
34. • Ramesh, A. V., Devi, D. R., Battu, G., & Basavaiah, K. (2018). A facile plant mediated synthesis of silver nanoparticles using an aqueous leaf extract of Ficus hispida Linn. f. for catalytic, antioxidant and antibacterial applications. South African Journal of Chemical Engineering, 26, 25–34.
35. • Qiao, M., Ying, G. G., Singer, A. C., & Zhu, Y. G. (2018). Review of antibiotic resistance in China and its environment. Environment International, 110, 160–172.
36. • Roy, A., Bulut, O., Some, S., Mandal, A. K., & Yilmaz, M. D. (2019). Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Advances, 9(5), 2673–2702.
37. • Russell, A. D., & Furr, J. R. (1977). The antibacterial activity of a new chloroxylenol preparation containing ethylenediamine tetraacetic acid. Journal of Applied Bacteriology, 43(2), 253–260.
38. • Santos, A., Barros, L., Calhelha, R. C., Dueñas, M., Carvalho, A. M., Santos-Buelga, C., & Ferreira, I. C. (2013). Leaves and decoction of Juglans regia L.: Different performances regarding bioactive compounds and in vitro antioxidant and antitumor effects. Industrial Crops and Products, 51, 430–436.
39. • Shah, T. I., Ganesh, N., & Akthar, S. (2013). Preliminary phytochemical evaluation and antibacterial potential of different leaf extracts of Juglana Regia: A ubiquitous dry fruit from Kashmir- India. Pharm Sci Rev Res, 19, 93–96.
40. • Shah, M., Fawcett, D., Sharma, S., Tripathy, S. K., & Poinern, G. E. J. (2015). Green synthesis of metallic nanoparticles via biological entities. Materials, 8(11), 7278–7308.
41. • Sievert, D. M., Ricks, P., Edwards, J. R., Schneider, A., Patel, J., Srinivasan, A., ... Fridkin, S. (2013). Antimicrobial-resistant pathogens associated with healthcare-associated infections summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009–2010. Infection Control & Hospital Epidemiology, 34(1), 1–14.
42. • Some, S., Bulut, O., Biswas, K., Kumar, A., Roy, A., Sen, I. K., ... Das, S. (2019). Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L.(Lepidoptera: Bombycidae). Scientific Reports, 9(1), 1–13.
43. • Strayer, A., Ocsoy, I., Tan, W., Jones, J. B., & Paret, M. L. (2016). Low concentrations of a silver-based nanocomposite to manage bacterial spot of tomato in the greenhouse. Plant Disease, 100(7), 1460–1465.
44. • Topacoglu, O. (2013). Genetic diversity among populations in Black Pine (Pinus nigra Arnold. subsp. pallasiana (Lamb.) Holmboe) seed stands in Turkey. Bulgarian Journal of Agricultural Science, 19(6), 1459–1464.
45. • Wang, Z., Liu, S., Ma, J., Qu, G., Wang, X., Yu, S., ... Jiang, G. B. (2013). Silver nanoparticles induced RNA polymerase-silver binding and RNA transcription inhibition in erythroid progenitor cells. ACS Nano, 7(5), 4171–4186.
46. • Weiner, L. M., Webb, A. K., Limbago, B., Dudeck, M. A., Patel, J., Kallen, A. J., ... Sievert, D. M. (2016). Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2011–2014. Infection Control & Hospital Epidemiology, 37(11), 1288–1301.
47. • Wright, G. D. (2010). Antibiotic resistance in the environment: a link to the clinic?. Current Opinion in Microbiology, 13(5), 589–594.
48. • You, C., Han, C., Wang, X., Zheng, Y., Li, Q., Hu, X., Sun, H. (2012). The progress of silver nanoparticles in the antibacterial mechanism, clinical application and cytotoxicity. Molecular Biology Reports, 39(9), 9193–9201.
49. • Zhao, M. H., Jiang, Z. T., Liu, T., & Li, R. (2014). Flavonoids in Juglans regia L. leaves and evaluation of in vitro antioxidant activity via intracellular and chemical methods. The Scientific World Journal, 2014, 1–6.