Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study

Emrah Dural; Sema Tülay Köz; Süleyman Köz

Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study

Abstract

Background and Aims: It has been observed that the combined treatment of esomeprazole and pirfenidone provides increased efficacy in the treatment of pulmonary fibrosis disease, recently. The aim of this study is to develop a simple, sensitive, and reliable high-performance liquid chromatography method to be used in drug monitoring to increase the effectiveness of esomeprazole and pirfenidone in treatment and to reduce their adverse effects. Methods: Separation was conducted with a C18 reverse-phase column (4.6 mm x 250 mm, 5 μm) used as a mobile phase prepared with the phosphate buffer (10 mM KH2PO4 and 10 mM K2HPO4) and acetonitrile (60:40, v/v) by an isocratic flow (1 mL/min). Mobile phase pH was adjusted to 3.0. Ultraviolet detection was accomplished at 305 nm. The column oven was held at 35°C to ensure an efficient analytical separation. Results: Analytical recovery of esomeprazole was between 92.43 and 105.36% and for pirfenidone it was found between 89.56 and 104.32%. Accuracy values of esomeprazole and pirfenidone were determined between (-2.90) – 4.22 and (-4.45) – 5.78, respectively. Precision (RSD%) was ≤7.89. The quantification limit was determined as 0.58 and 0.36 ng/mL. Plasma esomeprazole and pirfenidone levels were found as 0.87-8296.87 ng/mL (612.99±2212.20, mean ± standard deviation) and 0.45-238.60 ng/mL (61.44±76.35, mean ± standard deviation), respectively. Conclusion: Unexpectedly high RSD values were observed in both plasma (360.88%) and dose-rated results (89.61%) of esomeprazole, and pirfenidone were thought to be related to individual metabolism differences.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences, Health Care Administration

Journal Section

Research Article

Authors

Emrah Dural ^* This is me
0000-0002-9320-8008
Türkiye

Sema Tülay Köz This is me
0000-0002-3809-1070
Türkiye

Süleyman Köz This is me
0000-0001-5036-0475
Türkiye

Publication Date

April 30, 2021

Submission Date

September 18, 2020

Acceptance Date

December 21, 2020

Published in Issue

Year 2021 Volume: 51 Number: 1

IZ

https://izlik.org/JA43TK48TX

Cite

RIS / Bibtex

APA

Dural, E., Köz, S. T., & Köz, S. (2021). Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study. İstanbul Journal of Pharmacy, 51(1), 16-25. https://izlik.org/JA43TK48TX

AMA

1.Dural E, Köz ST, Köz S. Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study. iujp. 2021;51(1):16-25. https://izlik.org/JA43TK48TX

Chicago

Dural, Emrah, Sema Tülay Köz, and Süleyman Köz. 2021. “Development and Validation an HPLC - UV Method for Determination of Esomeprazole and Pirfenidone Simultaneously in Rat Plasma: Application to a Drug Monitoring Study”. İstanbul Journal of Pharmacy 51 (1): 16-25. https://izlik.org/JA43TK48TX.

EndNote

Dural E, Köz ST, Köz S (April 1, 2021) Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study. İstanbul Journal of Pharmacy 51 1 16–25.

IEEE

[1]E. Dural, S. T. Köz, and S. Köz, “Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study”, iujp, vol. 51, no. 1, pp. 16–25, Apr. 2021, [Online]. Available: https://izlik.org/JA43TK48TX

ISNAD

Dural, Emrah - Köz, Sema Tülay - Köz, Süleyman. “Development and Validation an HPLC - UV Method for Determination of Esomeprazole and Pirfenidone Simultaneously in Rat Plasma: Application to a Drug Monitoring Study”. İstanbul Journal of Pharmacy 51/1 (April 1, 2021): 16-25. https://izlik.org/JA43TK48TX.

JAMA

1.Dural E, Köz ST, Köz S. Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study. iujp. 2021;51:16–25.

MLA

Dural, Emrah, et al. “Development and Validation an HPLC - UV Method for Determination of Esomeprazole and Pirfenidone Simultaneously in Rat Plasma: Application to a Drug Monitoring Study”. İstanbul Journal of Pharmacy, vol. 51, no. 1, Apr. 2021, pp. 16-25, https://izlik.org/JA43TK48TX.

Vancouver

1.Emrah Dural, Sema Tülay Köz, Süleyman Köz. Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study. iujp [Internet]. 2021 Apr. 1;51(1):16-25. Available from: https://izlik.org/JA43TK48TX