Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr.

Seçil Sarıkaya Aydın; Vahap Murat Kutluay; Toshiaki Makino; Makoto Inoue; Ümmühan Şebnem Harput; İclal Saraçoğlu

Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr.

Abstract

Background and Aims: Hypericum microcalycinum Boiss. & Heldr. is used for inflammatory diseases in Anatolia. The involvement of liver X receptors (LXRs) and free radicals in inflammatory diseases, activation of LXRs, and high radical contents in cancerous tissue and organs prompted us to determine the radical scavenging and LXRα agonist activity of aqueous fraction of methanol extract and fractions of H. microcalycinum along with the isolation studies from active fractions. Methods: Isolation studies were carried out on chromatographic techniques. DPPH, NO, and SO radical scavenging activity methods were used for the determination of antioxidant activity, and a LXRE reporter gene assay was used for the determination of LXRα agonist activity. Results: While the extract showed weak LXRα agonist activity, phenolic compounds- rich fractions showed moderate activity. DPPH radical scavenging capacities of the extract and some fractions seemed to be very high as well as some isolated compounds. Bioactivity- guided studies resulted in the isolation of catechin (1), epicatechin (2), apigenin-8-C-(2-O-acetyl)- glucopyranoside (3), quercetin-3-O-β-glucopyranoside (4), quercetin-3-O-β-arabinopyranoside (5), kaempferol-3-O-β- arabinopyranoside (6), luteolin-8-C-β-glucopyranoside (orientin) (7). Conclusion: According to our results, compounds 1, 2, 4, and 5 may be responsible for the anti-inflammatory effects of H. microcalycinum as a function of LXRα agonist and free radical scavenging effects.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences, Health Care Administration

Journal Section

Research Article

Authors

Seçil Sarıkaya Aydın This is me
0000-0003-4692-9117
Türkiye

Vahap Murat Kutluay ^* This is me
0000-0003-4135-3497
Türkiye

Toshiaki Makino This is me
0000-0002-2524-8745
Japan

Makoto Inoue This is me
0000-0003-0116-320X
Japan

Ümmühan Şebnem Harput This is me
0000-0002-2641-3263
Türkiye

İclal Saraçoğlu This is me
0000-0003-0555-6262
Türkiye

Publication Date

April 30, 2021

Submission Date

July 6, 2020

Acceptance Date

December 3, 2020

Published in Issue

Year 2021 Volume: 51 Number: 1

IZ

https://izlik.org/JA67DJ29BG

Cite

RIS / Bibtex

APA

Sarıkaya Aydın, S., Kutluay, V. M., Makino, T., Inoue, M., Harput, Ü. Ş., & Saraçoğlu, İ. (2021). Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr. İstanbul Journal of Pharmacy, 51(1), 98-104. https://izlik.org/JA67DJ29BG

AMA

1.Sarıkaya Aydın S, Kutluay VM, Makino T, Inoue M, Harput ÜŞ, Saraçoğlu İ. Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr. iujp. 2021;51(1):98-104. https://izlik.org/JA67DJ29BG

Chicago

Sarıkaya Aydın, Seçil, Vahap Murat Kutluay, Toshiaki Makino, Makoto Inoue, Ümmühan Şebnem Harput, and İclal Saraçoğlu. 2021. “Isolation of Potential Liver X Receptor Alpha Agonist and Antioxidant Compounds from Hypericum Microcalycinum Boiss. & Heldr”. İstanbul Journal of Pharmacy 51 (1): 98-104. https://izlik.org/JA67DJ29BG.

EndNote

Sarıkaya Aydın S, Kutluay VM, Makino T, Inoue M, Harput ÜŞ, Saraçoğlu İ (April 1, 2021) Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr. İstanbul Journal of Pharmacy 51 1 98–104.

IEEE

[1]S. Sarıkaya Aydın, V. M. Kutluay, T. Makino, M. Inoue, Ü. Ş. Harput, and İ. Saraçoğlu, “Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr”., iujp, vol. 51, no. 1, pp. 98–104, Apr. 2021, [Online]. Available: https://izlik.org/JA67DJ29BG

ISNAD

Sarıkaya Aydın, Seçil - Kutluay, Vahap Murat - Makino, Toshiaki - Inoue, Makoto - Harput, Ümmühan Şebnem - Saraçoğlu, İclal. “Isolation of Potential Liver X Receptor Alpha Agonist and Antioxidant Compounds from Hypericum Microcalycinum Boiss. & Heldr”. İstanbul Journal of Pharmacy 51/1 (April 1, 2021): 98-104. https://izlik.org/JA67DJ29BG.

JAMA

1.Sarıkaya Aydın S, Kutluay VM, Makino T, Inoue M, Harput ÜŞ, Saraçoğlu İ. Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr. iujp. 2021;51:98–104.

MLA

Sarıkaya Aydın, Seçil, et al. “Isolation of Potential Liver X Receptor Alpha Agonist and Antioxidant Compounds from Hypericum Microcalycinum Boiss. & Heldr”. İstanbul Journal of Pharmacy, vol. 51, no. 1, Apr. 2021, pp. 98-104, https://izlik.org/JA67DJ29BG.

Vancouver

1.Seçil Sarıkaya Aydın, Vahap Murat Kutluay, Toshiaki Makino, Makoto Inoue, Ümmühan Şebnem Harput, İclal Saraçoğlu. Isolation of potential liver x receptor alpha agonist and antioxidant compounds from Hypericum microcalycinum Boiss. & Heldr. iujp [Internet]. 2021 Apr. 1;51(1):98-104. Available from: https://izlik.org/JA67DJ29BG