In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash

Mbang Nyong Femi-oyewo; Olutayo Ademola Adeleye; Caroline Olufunke Babalola; Olufemi Babatunde Banjo; Modupe Nofisat Adebowale; Florence Olubola Odeleye

In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash

Abstract

Background and Aims: Mouthwashes are oral home care product used to treat mouth infections and to maintain satisfactory oral hygiene. The use of effective mouthwash is a reasonable means to prevent dental caries and oro-dental infections. The aim of this study was to evaluate the antimicrobial potential of Distemonanthus benthamianus chewing stick extract formulated as a mouthwash against some microorganisms associated with oro-dental infections in comparison with two commercial brand mouthwashes. Methods: The chewing stick was extracted with methanol and used to formulate mouthwash. Phytochemical screening was done to test for the presence of secondary metabolites. Antimicrobial activity of the crude extract and the formulated mouthwash on Staphylococcus aureus, Streptococcus mutans and Candida albicans was evaluated. The physical characteristics, pH and the stability of the mouthwashes were determined. Results: The extract contains alkaloids, saponins flavonoids, steroids and tannins. The crude extract and the formulated mouthwashes showed significant difference in activity against the test microorganisms in the following order Candida albicans > Staphylococcus aureus > Streptococcus mutans. At high concentrations of the extract (0.3%W/V and 0.5%W/V) in formulations, antibacterial activities were significantly higher against Streptococcus mutans and Staphylococcus aureus when compared with the commercial brands. The pH of the mouthwashes ranged from 5.2 - 7.4. The stability of formulation F5 was maintained for 12 weeks. Conclusion: The use of Distemonanthus benthamianus chewing stick extract mouthwash is safer, cheaper, and more effective in preventing dental caries and oro-dental infections compared to the commercial brand mouthwashes.

Keywords

References

• Adeleye, O.A., Babalola, C.O., Femi-Oyewo, M.N., & Balogun, G.Y. (2019). Antimicrobial activity and stability of Andrographis paniculata cream containing shea butter. Nigerian Journal of Pharmaceutical Research, 15, 9-18.
• Adeniyi, B.A., Obasi, O.J., & Lawal, T. (2011). In vitro antifungal activity of Distemonanthus benthamianus stem. International Journal of Pharmacy and Pharmaceutical Sciences, 3, 52-56.
• Adeniyi, C.B., & Odumosu, B.T. (2012). Antibacterial and antifungal properties of Distemonanthus benthamianus (Fabaceae) crude extract. Global Journal of Pharmaceutical Research, 1, 567-574.
• Adeniyi, T.A., Adeonipekun, P.A,, & Omotayo, E.A. (2014). Investigating the phytochemicals and antimicrobial properties of three sedge (Cyperaceae) species. Notulae Scientia Biologicae, 6, 276-281.
• Aiyegoro, O.A., Akinpelu, D.A., Afolayan, A.J., & Okoh, A.I. (2008). Antibacterial activities of crude stem bark extracts of Distemonanthus benthamianus. Journal of Biological Sciences, 8, 356-361.
• Almekhlafi, S., Thabit, A.M., Alwossabi, M.I., Awadth, N., Thabe, A.M., & Algaadari, Z. (2014). Antimicrobial activity of Yemeni myrrh mouthwash. Journal of Chemical and Pharmaceutical Research, 6, 1006-1013.
• Asamoah, A., Frimpong-Mensah, K., & Antwi-Boasiako, C. (2014). Efficacy of Erythropleum suaveolens (potrodom) and Distemonanthus benthamianus (bonsamdua) water extractives on the durability of five Ghanaian less used timber species. Journal of Indian Academy of Wood Science, 11, 72–81.
• Aworinde, D.O., Erinoso, S.M., & IbukunOluwa, M.R. (2016). Mineral compositions, phytochemical constituents and in vitro antimicrobial screening of some chewing sticks from Ibadan, Nigeria. Journal of Applied Bioscience, 101, 9589-9597.

• Baliga, S., Muglikar, S., Kale, R. (2013). Salivary pH: A diagnostic biomarker. Journal of Indian Society of Periodontology, 17, 461‐465.
• Belardinelli, P.A., Morelatto, R.A., Benavidez, T.E., Baruzzi, A.M., & López de Blanc, S.A. (2014). Effect of two mouthwashes on salivary pH. Acta Odontology Latinoamericana, 27, 66-71.
• Dabholkar, C.S., Shah, M., Kathariya, R., Bajaj, M., & Doshi, Y. (2016). Comparative evaluation of antimicrobial activity of pomegranate- containing mouthwash against oral-biofilm forming microorganisms. Advances in Human Biology, 6, 99-103.
• Dave, P.H., Gurunathan, D., Vasantharajan, M.S. (2018). Comparison of pH levels of the saliva before and after the consumption of cough syrups in children. Biomedical and Pharmacology Journal, 11, 1443-1448.
• Hood, J.R., Wilkinson, J.M., & Cavanagh, H.M.A. (2003). Evaluation of common antibacterial screening methods utilized in essential oil research. Journal of Essential Oil Research, 15, 428-433.
• Khirtika, S.G., Ramesh, S., & Muralidharan, N.P. (2017). Comparative evaluation of antimicrobial efficacy of 0.2% Chlorhexidine, 2% iodine and homemade mouthrinse as an anti-caries agent. Journal of Pharmaceutical Science Research, 9, 2114-2116.
• Kunte, S., Kadam, N., Patel, A., Shah, P., Lodaya, R., & Lakde, L. (2018). Comparative evaluation of antimicrobial properties of pomegranate peel extract against Streptococcus mutans and Lactobacillus. International Dental and Medical Journal of Advanced Research. 4, 1-6.
• Malik, A.S., Shaukat, M.S., Qureshi, A.A., & Abdur, R. (2014). Comparative effectiveness of chewing stick and toothbrush: a randomized clinical trial. North American Journal of Medical Sciences, 6, 333‐337.
• Masadeh, M.M., Gharaibeh, S.F., Alzoubi, K.H., Al-Azzam, S.I., & Obeidat, W.M. (2013). Antimicrobial activity of common mouthwash solutions on multidrug-resistance bacterial biofilms. Journal of Clinical Medical Research, 5, 389-394.
• Mebude, O.O,, Adeniyi, B.A., & Lawal, T.O. (2017). In vitro antimicrobial activities of ethanol extract of Distemonanthus benthamianus (Aayan) Baillon (Fabaceae) on Streptococcus mutans. Journal of Advances in Medicine and Medical Research, 22, 1-8.
• Obiang, C.S., Misso, R.L., Atome, G.R., Ondo, J.P., Engonga, L.C., & Emvo, E.N. (2019). Phytochemical analyses, antimicrobial and antioxidant activities of stem bark extracts of Distemonanthus benthamianus H. Baill. and fruit extracts of Solanum torvum Sw. from Gabon. Asian Pacific Journal of Tropical Biomedicine, 9, 209-216.
• Parkar, S.M., Thakkar, P., & Shah, K. (2013). Antimicrobial activity of four commercially available mouthwashes against streptococcus Mutans: An in vitro study. Universal Research Journal of Dentistry, 3, 108-112.
• Saha, J.B., Pétrissans, A., Molina, S., Ndikontar, M.K., Mounguengui, S., Dumarçay, S., & Gérardin, P. (2014). Study of the feasibility of a natural dye on cellulosic textile supports by red padouk (Pterocarpus soyauxii) and yellow movingui (Distemonanthus benthamianus) extracts. Industrial Crops and Products, 60, 291-297.
• Shittu, O.A., Aliyu, A., David, M.S., Njinga, N.S., & Ishaq, H.I. (2019). Potential antibacterial activity of two important local chewing sticks “Fagara zanthoxyloides and Distemonanthus benthamianus” along with antioxidant capacities. Dhaka University Journal of Pharmaceutical Sciences, 18, 223-232.
• Takahashi, N. (2005). Microbial ecosystem in the oral cavity: Metabolic diversity in an ecological niche and its relationship with oral diseases. International Congress Series, 1284, 103-112.
• Trease, A., & Evans, W.C. (1989). Pharmacognosy. 13th (ed). London: Balliene Tindiall. Pp. 345-6, 535-6, 772-3.
• Van Zyk, A.W., & Van Heerdem, W.F. (2010). Mouthwash: A review for South African health care workers. South African family practice. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5, 121-127.
• Yadav, R., & Yadav, S.K. (2013). Dental disease and its cure: A review. Asian Journal of Pharmaceutical and Clinical Research, 6, 16-20.
• Zhao, D., Tsoi, J.K., Wong, H.M., Chu, C.H., & Matinlinna, J.P. (2017). Pediatric over-the-counter (OTC) oral liquids can soften and erode enamel. Dentistry Journal, 5, 17-24.

Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences, Health Care Administration

Journal Section

Research Article

Authors

Mbang Nyong Femi-oyewo This is me
0000-0003-0025-2227
Nigeria

Olutayo Ademola Adeleye ^* This is me
0000-0001-8716-4064
Nigeria

Caroline Olufunke Babalola This is me
Nigeria

Olufemi Babatunde Banjo This is me
0000-0002-6970-516X
Nigeria

Modupe Nofisat Adebowale This is me
0000-0002-0944-0702
Nigeria

Florence Olubola Odeleye This is me
Nigeria

Publication Date

April 30, 2021

Submission Date

June 14, 2020

Acceptance Date

September 21, 2020

Published in Issue

Year 2021 Volume: 51 Number: 1

IZ

https://izlik.org/JA44YZ62NE

Cite

RIS / Bibtex

APA

Femi-oyewo, M. N., Adeleye, O. A., Babalola, C. O., Banjo, O. B., Adebowale, M. N., & Odeleye, F. O. (2021). In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash. İstanbul Journal of Pharmacy, 51(1), 105-110. https://izlik.org/JA44YZ62NE

AMA

1.Femi-oyewo MN, Adeleye OA, Babalola CO, Banjo OB, Adebowale MN, Odeleye FO. In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash. iujp. 2021;51(1):105-110. https://izlik.org/JA44YZ62NE

Chicago

Femi-oyewo, Mbang Nyong, Olutayo Ademola Adeleye, Caroline Olufunke Babalola, Olufemi Babatunde Banjo, Modupe Nofisat Adebowale, and Florence Olubola Odeleye. 2021. “In Vitro Evaluation of Antimicrobial Activity of Distemonanthus Benthamianus Chewing Stick Extract Mouthwash”. İstanbul Journal of Pharmacy 51 (1): 105-10. https://izlik.org/JA44YZ62NE.

EndNote

Femi-oyewo MN, Adeleye OA, Babalola CO, Banjo OB, Adebowale MN, Odeleye FO (April 1, 2021) In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash. İstanbul Journal of Pharmacy 51 1 105–110.

IEEE

[1]M. N. Femi-oyewo, O. A. Adeleye, C. O. Babalola, O. B. Banjo, M. N. Adebowale, and F. O. Odeleye, “In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash”, iujp, vol. 51, no. 1, pp. 105–110, Apr. 2021, [Online]. Available: https://izlik.org/JA44YZ62NE

ISNAD

Femi-oyewo, Mbang Nyong - Adeleye, Olutayo Ademola - Babalola, Caroline Olufunke - Banjo, Olufemi Babatunde - Adebowale, Modupe Nofisat - Odeleye, Florence Olubola. “In Vitro Evaluation of Antimicrobial Activity of Distemonanthus Benthamianus Chewing Stick Extract Mouthwash”. İstanbul Journal of Pharmacy 51/1 (April 1, 2021): 105-110. https://izlik.org/JA44YZ62NE.

JAMA

1.Femi-oyewo MN, Adeleye OA, Babalola CO, Banjo OB, Adebowale MN, Odeleye FO. In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash. iujp. 2021;51:105–110.

MLA

Femi-oyewo, Mbang Nyong, et al. “In Vitro Evaluation of Antimicrobial Activity of Distemonanthus Benthamianus Chewing Stick Extract Mouthwash”. İstanbul Journal of Pharmacy, vol. 51, no. 1, Apr. 2021, pp. 105-10, https://izlik.org/JA44YZ62NE.

Vancouver

1.Mbang Nyong Femi-oyewo, Olutayo Ademola Adeleye, Caroline Olufunke Babalola, Olufemi Babatunde Banjo, Modupe Nofisat Adebowale, Florence Olubola Odeleye. In vitro evaluation of antimicrobial activity of Distemonanthus benthamianus chewing stick extract mouthwash. iujp [Internet]. 2021 Apr. 1;51(1):105-10. Available from: https://izlik.org/JA44YZ62NE