In vitro multiple pharmacological targets of Colutea cilicica Boiss. & Balansa against key enzymes linked to neurodegenerative diseases, diabetes, and hyperpigmentation

Year 2018, Volume: 48 Issue: 1, 18 - 24, 30.04.2018

Şengül Uysal Ramazan Ceylan Abdurrahman Aktumsek Gökalp Özmen Güler Carene Picot Gökhan Zengin , M. Fawzi Mahomoodally

Abstract

Prevention and treatment of noncommunicable
diseases such as neurodegenerative diseases, diabetes, and hyperpigmentation
using medicinal plants has attracted increasing attention during the past few
decades. In this study, Colutea cilicica Boiss. & Balansa extracts (ethyl
acetate, methanol, and water) were evaluated against key enzymes involved in
neurodegenerative diseases, diabetes, and hyperpigmentation. The antioxidant
(free radical scavenging, reducing power, β-carotene/linoleic acid, and
phosphomolybdenum) and metal chelation properties were also investigated. The
methanol extracts of C. cilicica vigorously inhibited the activities of
acetylcholinesterase and butyrylcholinesterase (1.33 and 0.68 mg galantamine
equivalents (GALAE)/g extract, respectively). It was observed that C. cilicica
extracts possessed a higher inhibitory potential for α-glucosidase (2.71–1.23
mmol acarbose equivalents (ACAE)/g extract) than that for α-amylase (0.57–0.12
mmol ACAE/g extract). The water extract of C. cilicica showed potent radical
scavenging capacity against DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS
(2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (42.46 and 57.70 mg
trolox equivalents (TE)/g extract, respectively). Phytochemical determination
showed that C. cilicica water extract (17.26 mg rutin equivalents (RE)/g
extract) was rich in flavonoids compared with ethyl acetate and methanol
extracts (2.78 and 2.83 mg RE/g extract, for the respective extracts). These
findings reveal the interesting potential of C. cilicica as a valuable source
of phytochemicals that can be used against common noncommunicable diseases,
particularly against enzymes involved in neurodegenerative diseases.

Keywords

Colutea cilica, Alzheimer’s disease, diabetes mellitus, natural agents, phytopharmaceuticals

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