Research Article
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In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds

Year 2020, Volume: 50 Issue: 3, 283 - 288, 30.12.2020

Aris Tercan Özlem Saçan

Abstract

Background and Aims: Adenosine deaminase (EC 3.5.4.4, ADA) is distributed in all human tissues. It catalyses the deamination of adenosine (deoxyadenosine) to inosine (deoxyinosine) via removal of an amino group. The determination of ADAinhibition is of extreme significance in the field of fundamental research and clinical diagnosis. In our study, the effects of various plant extracts and chemicals on ADA activity were investigated. Methods: Adenosine deaminase activity was determined spectrophotometrically at 625 nm. Results: The inhibitory activities of the extracts and chemical compounds increased in a dose-dependent manner. Among the plant extracts used, lemon extract was observed to exhibit the highest ADA inhibition activity with an IC50 value of 0.05 ± 0.001 mg/mL, while quercetin had the highest ADA inhibition (IC50 value of 0.004±0.0005 mg/mL) among the chemical compounds. All plant extracts and chemical compounds showed ADA inhibition activities. Conclusion: The obtained results indicate that plant extracts and some chemical compounds examined in this study can be a potential source of novel ADA-inhibitors for therapeutics.

Keywords

Adenosine deaminase enzyme inhibition plant extracts chemical compounds

References

  • Aiuti, A., Cattaneo, F., Galimberti, S., Benninghoff, U., Cassani, B., Callegaro, L., … Roncarolo, M. G. (2009). Gene therapy for immunodeficiency due to adenosine deaminase deficiency. New England Journal of Medicine, 360, 447–458. https://www.nejm.org/ doi/full/10.1056/NEJMoa0805817
  • Aldrich, M. B., Blackburn, M. R., & Kellems, R. E. (2000). The importance of adenosine deaminase for lymphocyte development and function. Biochemical and Biophysical Research Communications, 72, 311–315. https://doi.org/10.1006/bbrc.2000.2773
  • Alu’datt, M. H., Rababah, T., Alhamad, M. N., Al-Mahasneh, M. A., Ereifej, K., Al-Karaki, G., … Ghozlana, K. A. (2017). Profiles of free and bound phenolics extracted from Citrus fruits and their roles in biological systems: content, and antioxidant, anti-diabetic and anti-hypertensive properties. Food and Function, 8, 3187–3197. https://doi.org/10.1039/C7FO00212B
  • Anagnostopoulou, M. A., Kefalas, P., Papageorgiou, V. P., Assimopoulou, A. N., & Boskou, D. (2006). Radical scavenging activity of various extracts and fractions of sweet orange flavedo (Citrus sinensis). Food Chemistry, 94, 19–25. https://doi.org/10.1016/j.foodchem. 2004.09.047
  • Arruebo, M., Vilaboa, N., Sáez-Gutierrez, B., Lambea, J., Tres, A., Valladares, M., & González-Fernández, A. (2011). Assessment of the evolution of cancer treatment therapies. Cancers, 3, 3279–3330. http://dx.doi:10.3390/cancers3033279
  • Arun, K. G., Sharanyaa, C. S., Sandeepa, P. M., & Sadasivana, C. (2016). Inhibitory activity of hibifolin on adenosine deaminaseexperimental and molecular modeling study. Computational Biology and Chemistry, 64, 353–358. https://doi.org/10.1016/j.compbiolchem. 2016.08.005
  • Asencio, A. D., Serrano, M., Garcia‑Martinez, S., & Pretel, M. T. (2018). Organic acids, sugars, antioxidant activity, sensorial and other fruit characteristics of nine traditional Spanish citrus fruits. European Food Research and Technology, 244, 1497–1508. https:// doi.org/10.1007/s00217-018-3064-x
  • Blum, U., & Schwedt, G. (1998). Inhibition behavior of phosphatase, phosphodiesterase I and adenosine deaminase as tools for trace metal analysis and speciation. Analytica Chimica Acta, 360, 101–108. https://doi.org/10.1016/S0003-2670(97)00717-4
  • Canbolat, O., Akyol, O., Kavutcu, M., Isik, A. Ü., & Durak, I. (1994). Serum adenosine deaminase and total superoxide dismutase activities before and after surgical removal of cancerous laryngeal tissue. Journal of Laryngology and Otology, 108, 849–851. https:// doi.org/10.1017/S0022215100128300
  • Colditz, G. A., & Wei, E. K. (2012). Preventability of cancer: The relative contributions of biologic and social and physical environmental determinants of cancer mortality. Annual Review and Public Healthy, 33, 137–156. https://www.annualreviews.org/doi/ abs/10.1146/annurev-publhealth-031811-124627
  • Cha, J. Y., Cho, Y. S., Kim, I., Anno, T., Rahman, S. M., & Yanagita, T. (2001). Effect of hesperitin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Foods for Human Nutrition, 56, 349–358. Retrieved from https://www.ncbi.nlm.nih.gov/ pubmed/11678440
  • Dolezelova, E., Zurovec, M., Dolezal, T., Simek, P., & Bryant, P. J. (2005). The emerging role of adenosine deaminases in insects. Insect Biochemistry and Molecular Biology, 35, 381–389. https://doi. org/10.1016/j.ibmb.2004.12.009
  • Fleischman, A., Hershfield, M. S., Toutain, S., Lederman, H. M., Sullivan, K. E., Fasano, M. B., …Winkelstein, J. A. (1998). Adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency in common variable immunodeficiency. Clinical and Diagnostic Laboratory Immunology, 5, 399–400. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC104530
  • Gorinstein, S., Cvikrova, M., Machackova, I., Haruenkit, R., Park, Y. S., Jung, S. T., … Trakhtenberg, S. (2004). Characterization of antioxidant compounds in Jaffa sweeties and white grapefruits. Food Chemistry, 84, 503–510. https://doi.org/10.1016/S0308- 8146(03)00127-4
  • Guimarães, R., Barros, L., Barreira, J. C. M., Sousa, M. J., Carvalho, A. M., & Ferreira, I. C. F. R. (2009). Targeting excessive free radicals with peels and juices of citrus fruits: grapefruit, lemon, lime and orange. Food and Chemical Toxicology, 48, 99–106. https://doi. org/10.1016/j.fct.2009.09.022
  • Hoffbrand, A. V., & Janossy, G. (1981). Enzyme and membrane markers in leukaemia: recent developments. Journal of Clinical Pathology, 34, 254–262. Retrieved from https://jcp.bmj.com/content/ 34/3/254
  • İbiş, M., Koklu, S., Yilmaz, F. M., Basar, O., Yilmaz, G., Yuksel, O., … Ozturk, Z. A. (2007). Serum adenosine deaminase levels in pancreatic diseases. Pancreatology, 7, 526–530. https://doi. org/10.1159/000108970
  • Lal, H., Munjal, S. K., Wig, U., & Saini, A. S. (1987). Serum enzymes in head and neck cancer III. Journal of Laryngology and Otology, 101, 1062–1065. https://doi.org/10.1017/S0022215100103226
  • Markman, J. L., & Shiao, S. L. (2015). Impact of the immune system and immunotherapy in colorectal cancer. Journal of Gastrointestinal Oncology, 6(2), 208–223. https://doi.org/10.3978/j.issn.2078- 6891.2014.077
  • Musumeci, L., Maugeri, A., Cirmi, S., Lombardo, G. E., Russo, C., Gangemi, S., ... Navarra, M. (2020) Citrus fruits and their flavonoids in inflammatory bowel disease: an overview. Natural Product Research, 34, 122-136. https://doi.org/10.1080/14786419.2019.1601 196
  • Nishihara, H., Akedo, H., Okada, H., & Hattori, S. (1970). Multienzyme patterns of serum adenosine deaminase by agar gel electrophoresis: an evaluation of the diagnostic value in lung cancer. Clinica Chimica Acta, 30, 251–258. https://doi.org/10.1016/0009- 8981(70)90110-5
  • Ozdemir, A., Kaplan Serin, E., & Savas, M. (2018). Cancer risk factors and prevention in Turkey. International Journalof Health Services Research and Policy, 3, 143-150. https://doi.org/10.23884/ ijhsrp.2018.3.3.06
  • Portenoy, R. K., & Ahmed, E. (2018). Cancer pain syndromes. Hematology / Oncology Clinics, 32, 371–386. https://doi.org/10.1016/j. hoc.2018.01.002
  • Proteggente, A. R., Saija, A., De Pasquale, A., & Rice-Evans, C. A. (2003). The compositional characterisation and antioxidant activity of fresh juices from Sicilian sweet orange (Citrus sinensis L. Osbeck) varieties. Free Radicical Research, 37, 681–687. https://doi. org/10.1080/1071576031000083198
  • Russo, M. M., & Sundaramurthi, T. (2019). An overview of cancer pain: Epidemiology and pathophysiology. Seminars in Oncology Nursing, 35, 223–228. https://doi.org/10.1016/j.soncn.2019.04.002
  • Soerjomataram, I., Kevin Shield, K., Marant-Micallef, C., Vignat, J., Hill, C., Rogel, A., … Bray, F. (2018). Cancers related to lifestyle and environmental factors in France in 2015. European Journal of Cancer, 105,103–113. https://doi.org/10.1016/j.ejca.2018.09.009
  • Van der Weyden, M. B., & Kelley, W. N. (1976). Human adenosine deaminase. Journal of Biological Chemistry, 251(18), 5448–5456. Retrieved from https://www.jbc.org/content/251/18/5448
  • Walia, M., Mahajan, M., & Singh, K. (1995). Serum adenosine deaminase, 5'-nucleotidase & alkaline phosphatase in breast cancer patients. The Indian Journal of Medical Research, 101, 247–249. Retrieved from https://europepmc.org/article/med/7672835
  • Willett W. C. (2000). Diet and cancer. The Oncologist; 5, 393–404. https://doi.org/10.1634/theoncologist.5-5-393
  • Wang, M., Chen, J., Su, D., Wang, G., & Su, X. (2019). Split aptamer based sensing platform for adenosine deaminase detection by fluorescence resonance energy transfer. Talanta, 198, 1–7. https:// doi.org/10.1016/j.talanta.2019.01.041
  • Zaigham, A., & Sakina, R. (2018). An overview of cancer treatment modalities. In HN Shahzad (Ed.), Neoplasm (pp 139-157), London, UK: Intechopen Ebook Press.

Year 2020, Volume: 50 Issue: 3, 283 - 288, 30.12.2020

Aris Tercan Özlem Saçan

Abstract

References

  • Aiuti, A., Cattaneo, F., Galimberti, S., Benninghoff, U., Cassani, B., Callegaro, L., … Roncarolo, M. G. (2009). Gene therapy for immunodeficiency due to adenosine deaminase deficiency. New England Journal of Medicine, 360, 447–458. https://www.nejm.org/ doi/full/10.1056/NEJMoa0805817
  • Aldrich, M. B., Blackburn, M. R., & Kellems, R. E. (2000). The importance of adenosine deaminase for lymphocyte development and function. Biochemical and Biophysical Research Communications, 72, 311–315. https://doi.org/10.1006/bbrc.2000.2773
  • Alu’datt, M. H., Rababah, T., Alhamad, M. N., Al-Mahasneh, M. A., Ereifej, K., Al-Karaki, G., … Ghozlana, K. A. (2017). Profiles of free and bound phenolics extracted from Citrus fruits and their roles in biological systems: content, and antioxidant, anti-diabetic and anti-hypertensive properties. Food and Function, 8, 3187–3197. https://doi.org/10.1039/C7FO00212B
  • Anagnostopoulou, M. A., Kefalas, P., Papageorgiou, V. P., Assimopoulou, A. N., & Boskou, D. (2006). Radical scavenging activity of various extracts and fractions of sweet orange flavedo (Citrus sinensis). Food Chemistry, 94, 19–25. https://doi.org/10.1016/j.foodchem. 2004.09.047
  • Arruebo, M., Vilaboa, N., Sáez-Gutierrez, B., Lambea, J., Tres, A., Valladares, M., & González-Fernández, A. (2011). Assessment of the evolution of cancer treatment therapies. Cancers, 3, 3279–3330. http://dx.doi:10.3390/cancers3033279
  • Arun, K. G., Sharanyaa, C. S., Sandeepa, P. M., & Sadasivana, C. (2016). Inhibitory activity of hibifolin on adenosine deaminaseexperimental and molecular modeling study. Computational Biology and Chemistry, 64, 353–358. https://doi.org/10.1016/j.compbiolchem. 2016.08.005
  • Asencio, A. D., Serrano, M., Garcia‑Martinez, S., & Pretel, M. T. (2018). Organic acids, sugars, antioxidant activity, sensorial and other fruit characteristics of nine traditional Spanish citrus fruits. European Food Research and Technology, 244, 1497–1508. https:// doi.org/10.1007/s00217-018-3064-x
  • Blum, U., & Schwedt, G. (1998). Inhibition behavior of phosphatase, phosphodiesterase I and adenosine deaminase as tools for trace metal analysis and speciation. Analytica Chimica Acta, 360, 101–108. https://doi.org/10.1016/S0003-2670(97)00717-4
  • Canbolat, O., Akyol, O., Kavutcu, M., Isik, A. Ü., & Durak, I. (1994). Serum adenosine deaminase and total superoxide dismutase activities before and after surgical removal of cancerous laryngeal tissue. Journal of Laryngology and Otology, 108, 849–851. https:// doi.org/10.1017/S0022215100128300
  • Colditz, G. A., & Wei, E. K. (2012). Preventability of cancer: The relative contributions of biologic and social and physical environmental determinants of cancer mortality. Annual Review and Public Healthy, 33, 137–156. https://www.annualreviews.org/doi/ abs/10.1146/annurev-publhealth-031811-124627
  • Cha, J. Y., Cho, Y. S., Kim, I., Anno, T., Rahman, S. M., & Yanagita, T. (2001). Effect of hesperitin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Foods for Human Nutrition, 56, 349–358. Retrieved from https://www.ncbi.nlm.nih.gov/ pubmed/11678440
  • Dolezelova, E., Zurovec, M., Dolezal, T., Simek, P., & Bryant, P. J. (2005). The emerging role of adenosine deaminases in insects. Insect Biochemistry and Molecular Biology, 35, 381–389. https://doi. org/10.1016/j.ibmb.2004.12.009
  • Fleischman, A., Hershfield, M. S., Toutain, S., Lederman, H. M., Sullivan, K. E., Fasano, M. B., …Winkelstein, J. A. (1998). Adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency in common variable immunodeficiency. Clinical and Diagnostic Laboratory Immunology, 5, 399–400. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC104530
  • Gorinstein, S., Cvikrova, M., Machackova, I., Haruenkit, R., Park, Y. S., Jung, S. T., … Trakhtenberg, S. (2004). Characterization of antioxidant compounds in Jaffa sweeties and white grapefruits. Food Chemistry, 84, 503–510. https://doi.org/10.1016/S0308- 8146(03)00127-4
  • Guimarães, R., Barros, L., Barreira, J. C. M., Sousa, M. J., Carvalho, A. M., & Ferreira, I. C. F. R. (2009). Targeting excessive free radicals with peels and juices of citrus fruits: grapefruit, lemon, lime and orange. Food and Chemical Toxicology, 48, 99–106. https://doi. org/10.1016/j.fct.2009.09.022
  • Hoffbrand, A. V., & Janossy, G. (1981). Enzyme and membrane markers in leukaemia: recent developments. Journal of Clinical Pathology, 34, 254–262. Retrieved from https://jcp.bmj.com/content/ 34/3/254
  • İbiş, M., Koklu, S., Yilmaz, F. M., Basar, O., Yilmaz, G., Yuksel, O., … Ozturk, Z. A. (2007). Serum adenosine deaminase levels in pancreatic diseases. Pancreatology, 7, 526–530. https://doi. org/10.1159/000108970
  • Lal, H., Munjal, S. K., Wig, U., & Saini, A. S. (1987). Serum enzymes in head and neck cancer III. Journal of Laryngology and Otology, 101, 1062–1065. https://doi.org/10.1017/S0022215100103226
  • Markman, J. L., & Shiao, S. L. (2015). Impact of the immune system and immunotherapy in colorectal cancer. Journal of Gastrointestinal Oncology, 6(2), 208–223. https://doi.org/10.3978/j.issn.2078- 6891.2014.077
  • Musumeci, L., Maugeri, A., Cirmi, S., Lombardo, G. E., Russo, C., Gangemi, S., ... Navarra, M. (2020) Citrus fruits and their flavonoids in inflammatory bowel disease: an overview. Natural Product Research, 34, 122-136. https://doi.org/10.1080/14786419.2019.1601 196
  • Nishihara, H., Akedo, H., Okada, H., & Hattori, S. (1970). Multienzyme patterns of serum adenosine deaminase by agar gel electrophoresis: an evaluation of the diagnostic value in lung cancer. Clinica Chimica Acta, 30, 251–258. https://doi.org/10.1016/0009- 8981(70)90110-5
  • Ozdemir, A., Kaplan Serin, E., & Savas, M. (2018). Cancer risk factors and prevention in Turkey. International Journalof Health Services Research and Policy, 3, 143-150. https://doi.org/10.23884/ ijhsrp.2018.3.3.06
  • Portenoy, R. K., & Ahmed, E. (2018). Cancer pain syndromes. Hematology / Oncology Clinics, 32, 371–386. https://doi.org/10.1016/j. hoc.2018.01.002
  • Proteggente, A. R., Saija, A., De Pasquale, A., & Rice-Evans, C. A. (2003). The compositional characterisation and antioxidant activity of fresh juices from Sicilian sweet orange (Citrus sinensis L. Osbeck) varieties. Free Radicical Research, 37, 681–687. https://doi. org/10.1080/1071576031000083198
  • Russo, M. M., & Sundaramurthi, T. (2019). An overview of cancer pain: Epidemiology and pathophysiology. Seminars in Oncology Nursing, 35, 223–228. https://doi.org/10.1016/j.soncn.2019.04.002
  • Soerjomataram, I., Kevin Shield, K., Marant-Micallef, C., Vignat, J., Hill, C., Rogel, A., … Bray, F. (2018). Cancers related to lifestyle and environmental factors in France in 2015. European Journal of Cancer, 105,103–113. https://doi.org/10.1016/j.ejca.2018.09.009
  • Van der Weyden, M. B., & Kelley, W. N. (1976). Human adenosine deaminase. Journal of Biological Chemistry, 251(18), 5448–5456. Retrieved from https://www.jbc.org/content/251/18/5448
  • Walia, M., Mahajan, M., & Singh, K. (1995). Serum adenosine deaminase, 5'-nucleotidase & alkaline phosphatase in breast cancer patients. The Indian Journal of Medical Research, 101, 247–249. Retrieved from https://europepmc.org/article/med/7672835
  • Willett W. C. (2000). Diet and cancer. The Oncologist; 5, 393–404. https://doi.org/10.1634/theoncologist.5-5-393
  • Wang, M., Chen, J., Su, D., Wang, G., & Su, X. (2019). Split aptamer based sensing platform for adenosine deaminase detection by fluorescence resonance energy transfer. Talanta, 198, 1–7. https:// doi.org/10.1016/j.talanta.2019.01.041
  • Zaigham, A., & Sakina, R. (2018). An overview of cancer treatment modalities. In HN Shahzad (Ed.), Neoplasm (pp 139-157), London, UK: Intechopen Ebook Press.
There are 31 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences, Health Care Administration
Journal Section Original Article
Authors

Aris Tercan This is me 0000-0003-4805-5337

Özlem Saçan This is me 0000-0001-6503-4613

Publication Date December 30, 2020
Submission Date January 14, 2020
Published in Issue Year 2020 Volume: 50 Issue: 3

Cite

APA Tercan, A., & Saçan, Ö. (2020). In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds. İstanbul Journal of Pharmacy, 50(3), 283-288.
AMA Tercan A, Saçan Ö. In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds. iujp. December 2020;50(3):283-288.
Chicago Tercan, Aris, and Özlem Saçan. “In Vitro Adenosine Deaminase Inhibitory Activity of Some Selected Plant Extracts and Chemical Compounds”. İstanbul Journal of Pharmacy 50, no. 3 (December 2020): 283-88.
EndNote Tercan A, Saçan Ö (December 1, 2020) In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds. İstanbul Journal of Pharmacy 50 3 283–288.
IEEE A. Tercan and Ö. Saçan, “In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds”, iujp, vol. 50, no. 3, pp. 283–288, 2020.
ISNAD Tercan, Aris - Saçan, Özlem. “In Vitro Adenosine Deaminase Inhibitory Activity of Some Selected Plant Extracts and Chemical Compounds”. İstanbul Journal of Pharmacy 50/3 (December 2020), 283-288.
JAMA Tercan A, Saçan Ö. In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds. iujp. 2020;50:283–288.
MLA Tercan, Aris and Özlem Saçan. “In Vitro Adenosine Deaminase Inhibitory Activity of Some Selected Plant Extracts and Chemical Compounds”. İstanbul Journal of Pharmacy, vol. 50, no. 3, 2020, pp. 283-8.
Vancouver Tercan A, Saçan Ö. In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds. iujp. 2020;50(3):283-8.
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