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Year 2020, Volume: 50 Issue: 3, 289 - 293, 30.12.2020

Abstract

References

  • • Adaramola, B., & Onigbinde, A. (2016). Effect of extraction solvent on the phenolic content, flavonoid content and antioxidant capacity of clove bud. Article in IOSR Journal of Pharmacy and Biological Sciences, 11(2), 33–38.
  • • Alam, F., Shafique, Z., Amjad, S. T., & Bin Asad, M. H. H. (2019). Enzymes inhibitors from natural sources with antidiabetic activity: A review. Phytotherapy Research, 33(1), 41–54.
  • • Babbar, N., Oberoi, H. S., Uppal, D. S., & Patil, R. T. (2011). Total phenolic content and antioxidant capacity of extracts obtained from six important fruit residues. Food Research International, 44(1), 391–396.
  • • Baiano, A., Terracone, C., Viggiani, I., & Del Nobile, M. A. (2013). Effects of cultivars and location on quality, phenolic content and antioxidant activity of extra-virgin olive oils. JAOCS, Journal of the American Oil Chemists’ Society, 90(1), 103–111.
  • • Çakmak, Y. S., Zengin, G., Eskin, B., Yıldırım, K., Topal, M., Aydın, G. H. … Erten, K. (2017). Investigation of Antioxidant and Enzyme Inhibition Activities and Phenolic Compound of Medicago rigidula (L.) All. Marmara Pharmaceutical Journal, 21(3), 522–529.
  • • Fidrianny, I., Budiana, W., & Ruslan, K. (2015). Antioxidant activities of various extracts from ardisia sp leaves using dpph and cuprac assays and correlation with total flavonoid, phenolic, carotenoid content. International Journal of Pharmacognosy and Phytochemical Research, 7, 859–865.
  • • Francis, C. M., & Gillespie, D. J. (1981). Ecology and distribution of subterranean clover and Medicago species in Sardinia. Australian Plant Introduction Review, 13, 15–25.
  • • Ghimire, B. K., Ghimire, B., Yu, C. Y., & Chung, I.-M. (2019). Allelopathic and Autotoxic Effects of Medicago sativa—Derived Allelochemicals. Plants, 8(7), 233.
  • • Gholami, A., De Geyter, N., Pollier, J., Goormachtig, S., & Goossens, A. (2014). Natural product biosynthesis in Medicago species. Natural Product Reports, 31(3), 356–380.
  • • Gillespie, D. J., & McComb, J. A. (1991). Morphology and distribution of species in the Medicago murex complex. Canadian Journal of Botany, 69(12), 2655–2662.
  • • Hassan, M., Raza, H., Abbasi, M. A., Moustafa, A. A., & Seo, S.-Y. (2019). The exploration of novel Alzheimer’s therapeutic agents from the pool of FDA approved medicines using drug repositioning, enzyme inhibition and kinetic mechanism approaches. Biomedicine & Pharmacotherapy, 109, 2513–2526.
  • • Kicel, A., & Olszewska, M. A. (2015). Evaluation of antioxidant activity, and quantitative estimation of flavonoids, saponins and phenols in crude extract and dry fractions of Medicago lupulina aerial parts. Natural Product Communications, 10(3), 483–486.
  • • Martins, N., Barros, L., & Ferreira, I. C. F. R. (2016). In vivo antioxidant activity of phenolic compounds: Facts and gaps. Trends in Food Science & Technology, 48, 1–12.
  • • Mölgaard, J., von Schenck, H., & Olsson, A. G. (1987). Alfalfa seeds lower low density lipoprotein cholesterol and apolipoprotein B concentrations in patients with type II hyperlipoproteinemia. Atherosclerosis, 65(1–2), 173–179.
  • • Mortensen, A., Kulling, S. E., Schwartz, H., Rowland, I., Ruefer, C. E., Rimbach, G., Cassidy, A., Magee, P., Millar, J., & Hall, W. L. (2009). Analytical and compositional aspects of isoflavones in food and their biological effects. Molecular Nutrition & Food Research, 53(S2), S266–S309.
  • • Quideau, S., Deffieux, D., Douat-Casassus, C., & Pouységu, L. (2011). Plant polyphenols: Chemical properties, biological activities, and synthesis. In Angewandte Chemie - International Edition (pp. 586–621).
  • • Rodrigues, F., Palmeira-de-Oliveira, A., das Neves, J., Sarmento, B., Amaral, M. H., & Oliveira, M. B. (2013). Medicago spp. extracts as promising ingredients for skin care products. Industrial Crops and Products, 49, 634–644.
  • • Sudhakaran, M., Sardesai, S., & Doseff, A. I. (2019). Flavonoids: new frontier for immuno-regulation and breast cancer control. Antioxidants, 8(4), 103.
  • • Takyi, E. E. K., Kido, Y., Rikimaru, T., & Kennedy, D. O. (1992). Possible use of alfalfa (Medicago sativa) as supplement in infant nutrition: Comparison of weight gained by rats fed on alfalfa and a popular weaning diet. Journal of the Science of Food and Agriculture, 59(1), 109–115.
  • • Uysal, S., Zengin, G., Locatelli, M., Bahadori, M. B., Mocan, A., Bellagamba, G. … Aktumsek, A. (2017). Cytotoxic and enzyme inhibitory potential of two potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Frontiers in Pharmacology, 8, 290.
  • • Xu, D.-P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J.-J., & Li, H.-B. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18(1), 96.
  • • Zengin, G., Sieniawska, E., Senkardes, I., Picot-Allain, M. C. N., Sinan, K. I., & Mahomoodally, M. F. (2019). Antioxidant abilities, key enzyme inhibitory potential and phytochemical profile of Tanacetum poteriifolium Grierson. Industrial Crops and Products, 140, 111629.

Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae)

Year 2020, Volume: 50 Issue: 3, 289 - 293, 30.12.2020

Abstract

Background and Aims: Most of the Medicago species are considered good quality forage crops due to their rich protein content. This study reports antioxidant properties, total phenolic and flavonoid content, enzyme (acetylcholinesterase (AChE), tyrosinase, α-amylase and α-glucosidase) inhibition activities of methanol (MeOH), ethyl acetate (EA) and, aqueous (AQ) extracts of Medicago murex (M. murex). Methods: The antioxidant activity of the different extracts of M. murex were evaluated using FRAP and CUPRAC assays. The contents of total phenolic and flavonoid were determined by the Folin–Ciocalteu and the Aluminium chloride (AlCl3) colorimetric methods. Also, the enzyme inhibition activities were shown spectrophotometrically for AChE, α-amylase, α-glucosidase and tyrosinase enzymes. Results: Total phenolic and flavonoid contents of different extracts were determined between 83.70-163.93 mg GAE/g extract and 24.48-26.05 μg QE/g extract, respectively. AQ extracts were the most active extracts in FRAP while being the best inhibitor of the AChE enzyme. On the other hand, EA extracts were the most active extracts in CUPRAC methods and the best inhibitors for other enzymes. Conclusion: Considering the findings, M. murex appears to be an important source of natural antioxidants and enzyme inhibitors, and it provides important baseline data for the food and pharmaceutical industries.

References

  • • Adaramola, B., & Onigbinde, A. (2016). Effect of extraction solvent on the phenolic content, flavonoid content and antioxidant capacity of clove bud. Article in IOSR Journal of Pharmacy and Biological Sciences, 11(2), 33–38.
  • • Alam, F., Shafique, Z., Amjad, S. T., & Bin Asad, M. H. H. (2019). Enzymes inhibitors from natural sources with antidiabetic activity: A review. Phytotherapy Research, 33(1), 41–54.
  • • Babbar, N., Oberoi, H. S., Uppal, D. S., & Patil, R. T. (2011). Total phenolic content and antioxidant capacity of extracts obtained from six important fruit residues. Food Research International, 44(1), 391–396.
  • • Baiano, A., Terracone, C., Viggiani, I., & Del Nobile, M. A. (2013). Effects of cultivars and location on quality, phenolic content and antioxidant activity of extra-virgin olive oils. JAOCS, Journal of the American Oil Chemists’ Society, 90(1), 103–111.
  • • Çakmak, Y. S., Zengin, G., Eskin, B., Yıldırım, K., Topal, M., Aydın, G. H. … Erten, K. (2017). Investigation of Antioxidant and Enzyme Inhibition Activities and Phenolic Compound of Medicago rigidula (L.) All. Marmara Pharmaceutical Journal, 21(3), 522–529.
  • • Fidrianny, I., Budiana, W., & Ruslan, K. (2015). Antioxidant activities of various extracts from ardisia sp leaves using dpph and cuprac assays and correlation with total flavonoid, phenolic, carotenoid content. International Journal of Pharmacognosy and Phytochemical Research, 7, 859–865.
  • • Francis, C. M., & Gillespie, D. J. (1981). Ecology and distribution of subterranean clover and Medicago species in Sardinia. Australian Plant Introduction Review, 13, 15–25.
  • • Ghimire, B. K., Ghimire, B., Yu, C. Y., & Chung, I.-M. (2019). Allelopathic and Autotoxic Effects of Medicago sativa—Derived Allelochemicals. Plants, 8(7), 233.
  • • Gholami, A., De Geyter, N., Pollier, J., Goormachtig, S., & Goossens, A. (2014). Natural product biosynthesis in Medicago species. Natural Product Reports, 31(3), 356–380.
  • • Gillespie, D. J., & McComb, J. A. (1991). Morphology and distribution of species in the Medicago murex complex. Canadian Journal of Botany, 69(12), 2655–2662.
  • • Hassan, M., Raza, H., Abbasi, M. A., Moustafa, A. A., & Seo, S.-Y. (2019). The exploration of novel Alzheimer’s therapeutic agents from the pool of FDA approved medicines using drug repositioning, enzyme inhibition and kinetic mechanism approaches. Biomedicine & Pharmacotherapy, 109, 2513–2526.
  • • Kicel, A., & Olszewska, M. A. (2015). Evaluation of antioxidant activity, and quantitative estimation of flavonoids, saponins and phenols in crude extract and dry fractions of Medicago lupulina aerial parts. Natural Product Communications, 10(3), 483–486.
  • • Martins, N., Barros, L., & Ferreira, I. C. F. R. (2016). In vivo antioxidant activity of phenolic compounds: Facts and gaps. Trends in Food Science & Technology, 48, 1–12.
  • • Mölgaard, J., von Schenck, H., & Olsson, A. G. (1987). Alfalfa seeds lower low density lipoprotein cholesterol and apolipoprotein B concentrations in patients with type II hyperlipoproteinemia. Atherosclerosis, 65(1–2), 173–179.
  • • Mortensen, A., Kulling, S. E., Schwartz, H., Rowland, I., Ruefer, C. E., Rimbach, G., Cassidy, A., Magee, P., Millar, J., & Hall, W. L. (2009). Analytical and compositional aspects of isoflavones in food and their biological effects. Molecular Nutrition & Food Research, 53(S2), S266–S309.
  • • Quideau, S., Deffieux, D., Douat-Casassus, C., & Pouységu, L. (2011). Plant polyphenols: Chemical properties, biological activities, and synthesis. In Angewandte Chemie - International Edition (pp. 586–621).
  • • Rodrigues, F., Palmeira-de-Oliveira, A., das Neves, J., Sarmento, B., Amaral, M. H., & Oliveira, M. B. (2013). Medicago spp. extracts as promising ingredients for skin care products. Industrial Crops and Products, 49, 634–644.
  • • Sudhakaran, M., Sardesai, S., & Doseff, A. I. (2019). Flavonoids: new frontier for immuno-regulation and breast cancer control. Antioxidants, 8(4), 103.
  • • Takyi, E. E. K., Kido, Y., Rikimaru, T., & Kennedy, D. O. (1992). Possible use of alfalfa (Medicago sativa) as supplement in infant nutrition: Comparison of weight gained by rats fed on alfalfa and a popular weaning diet. Journal of the Science of Food and Agriculture, 59(1), 109–115.
  • • Uysal, S., Zengin, G., Locatelli, M., Bahadori, M. B., Mocan, A., Bellagamba, G. … Aktumsek, A. (2017). Cytotoxic and enzyme inhibitory potential of two potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Frontiers in Pharmacology, 8, 290.
  • • Xu, D.-P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J.-J., & Li, H.-B. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18(1), 96.
  • • Zengin, G., Sieniawska, E., Senkardes, I., Picot-Allain, M. C. N., Sinan, K. I., & Mahomoodally, M. F. (2019). Antioxidant abilities, key enzyme inhibitory potential and phytochemical profile of Tanacetum poteriifolium Grierson. Industrial Crops and Products, 140, 111629.
There are 22 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences, Health Care Administration
Journal Section Original Article
Authors

Damla Pamukcu This is me 0000-0001-5819-7449

Volkan Aylanç This is me 0000-0003-4060-766X

Bülent Eskin This is me 0000-0002-7990-4138

Gökhan Zengin This is me 0000-0001-6548-7823

Mehmet Dursun This is me 0000-0002-8214-4942

Yavuz Selim Çakmak This is me 0000-0001-8954-5485

Publication Date December 30, 2020
Submission Date May 2, 2020
Published in Issue Year 2020 Volume: 50 Issue: 3

Cite

APA Pamukcu, D., Aylanç, V., Eskin, B., Zengin, G., et al. (2020). Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae). İstanbul Journal of Pharmacy, 50(3), 289-293.
AMA Pamukcu D, Aylanç V, Eskin B, Zengin G, Dursun M, Çakmak YS. Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae). iujp. December 2020;50(3):289-293.
Chicago Pamukcu, Damla, Volkan Aylanç, Bülent Eskin, Gökhan Zengin, Mehmet Dursun, and Yavuz Selim Çakmak. “Investigation of in Vitro Anti-Hyperpigmentation, Antidiabetic, Neuroprotective and Antioxidant Potential of Medicago Murex WILLD. (Fabaceae)”. İstanbul Journal of Pharmacy 50, no. 3 (December 2020): 289-93.
EndNote Pamukcu D, Aylanç V, Eskin B, Zengin G, Dursun M, Çakmak YS (December 1, 2020) Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae). İstanbul Journal of Pharmacy 50 3 289–293.
IEEE D. Pamukcu, V. Aylanç, B. Eskin, G. Zengin, M. Dursun, and Y. S. Çakmak, “Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae)”, iujp, vol. 50, no. 3, pp. 289–293, 2020.
ISNAD Pamukcu, Damla et al. “Investigation of in Vitro Anti-Hyperpigmentation, Antidiabetic, Neuroprotective and Antioxidant Potential of Medicago Murex WILLD. (Fabaceae)”. İstanbul Journal of Pharmacy 50/3 (December 2020), 289-293.
JAMA Pamukcu D, Aylanç V, Eskin B, Zengin G, Dursun M, Çakmak YS. Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae). iujp. 2020;50:289–293.
MLA Pamukcu, Damla et al. “Investigation of in Vitro Anti-Hyperpigmentation, Antidiabetic, Neuroprotective and Antioxidant Potential of Medicago Murex WILLD. (Fabaceae)”. İstanbul Journal of Pharmacy, vol. 50, no. 3, 2020, pp. 289-93.
Vancouver Pamukcu D, Aylanç V, Eskin B, Zengin G, Dursun M, Çakmak YS. Investigation of in vitro anti-hyperpigmentation, antidiabetic, neuroprotective and antioxidant potential of Medicago murex WILLD. (Fabaceae). iujp. 2020;50(3):289-93.