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Year 2019, Volume: 49 Issue: 1, 25 - 32, 02.05.2019

Abstract

References

  • 1. Malvezzi, M., et al., European cancer mortality predictions for the year 2015: does lung cancer have the highest death rate in EU women. Ann Oncol, 2015. 26(4): p. 779-786.
  • 2. Harden, G.J., Flora of New South Wales. Vol. 4. 1990: UNSW Press.
  • 3. Oyedeji, O.O., et al., Chemical composition and antibacterial activity of the essential oils of Callistemon citrinus and Callistemon viminalis from South Africa. Molecules, 2009. 14(6): p. 1990-1998.
  • 4. Sudhakar, M., et al., Antinociceptive and anti-inflammatory effects of the standardized oil of Indian Callistemon lanceolatus leaves in experimental animals. East and Central African Journal of Pharmaceutical Sciences, 2004. 7(1): p. 10-15.
  • 5. Paluri, V., et al., Phytochemical composition and in vitro antimicrobial activity of methanolic extract of Callistemon lanceolatus DC. Int J Pharm Pharm Sci, 2012. 4(2): p. 699-2.
  • 6. Brodbeck, D., P. Cron, and B.A. Hemmings, A human protein kinase Bγ with regulatory phosphorylation sites in the activation loop and in the C-terminal hydrophobic domain. Journal of Biological Chemistry, 1999. 274(14): p. 9133-9136.
  • 7. Altomare, D.A., et al., Akt2 mRNA is highly expressed in embryonic brown fat and the AKT2 kinase is activated by insulin. Oncogene, 1998. 16(18): p. 2407-2411.
  • 8. Altomare, D.A., et al., Cloning, chromosomal localization and expression analysis of the mouse Akt2 oncogene. Oncogene, 1995. 11(6): p. 1055-1060.
  • 9. COFFER, P.J. and J.R. WOODGETT, Molecular cloning and characterisation of a novel putative protein‐serine kinase related to the cAMP‐dependent and protein kinase C families. European Journal of Biochemistry, 1991. 201(2): p. 475-481.
  • 10. Murthy, S., et al., Mapping of AKT3, encoding a member of the Akt/protein kinase B family, to human and rodent chromosomes by fluorescence in situ hybridization. Cytogenetic and Genome Research, 2000. 88(1-2): p. 38-40.
  • 11. Cheng, J.Q., et al., AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas. Proceedings of the National Academy of Sciences, 1992. 89(19): p. 9267-9271.
  • 12. Staal, S.P., et al., The AKT1 proto-oncogene maps to human chromosome 14, band q32. Genomics, 1988. 2(1): p. 96-98.
  • 13. Vivanco, I. and C.L. Sawyers, The phosphatidylinositol 3-kinase–AKT pathway in human cancer. Nature Reviews Cancer, 2002. 2(7): p. 489-501.
  • 14. Datta, S.R., A. Brunet, and M.E. Greenberg, Cellular survival: a play in three Akts. Genes & development, 1999. 13(22): p. 2905-2927.
  • 15. Blume-Jensen, P. and T. Hunter, Oncogenic kinase signalling. Nature, 2001. 411(6835): p. 355-365.
  • 16. Brognard, J., et al., Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer research, 2001. 61(10): p. 3986-3997.
  • 17. Kreisberg, J.I., et al., Phosphorylation of Akt (Ser473) is an excellent predictor of poor clinical outcome in prostate cancer. Cancer research, 2004. 64(15): p. 5232-5236.
  • 18. Grille, S.J., et al., The protein kinase Akt induces epithelial mesenchymal transition and promotes enhanced motility and invasiveness of squamous cell carcinoma lines. Cancer research, 2003. 63(9): p. 2172-2178.
  • 19. Sun, M., et al., Phosphatidylinositol-3-OH kinase (PI3K)/AKT2, activated in breast cancer, regulates and is induced by estrogen receptor α (ERα) via interaction between ERα and PI3K. Cancer research, 2001. 61(16): p. 5985-5991.
  • 20. Roy, H.K., et al., AKT proto-oncogene overexpression is an early event during sporadic colon carcinogenesis. Carcinogenesis, 2002. 23(1): p. 201-205.
  • 21. Itoh, N., et al., Phosphorylation of Akt/PKB is required for suppression of cancer cell apoptosis and tumor progression in human colorectal carcinoma. Cancer, 2002. 94(12): p. 3127-3134.
  • 22. Ke, N., et al., The xCELLigence system for real-time and label-free monitoring of cell viability. Mammalian Cell Viability: Methods and Protocols, 2011: p. 33-43.
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  • 24. Kumar, D., et al., Chemical composition and in vitro cytotoxicity of essential oils from leaves and flowers of Callistemon citrinus from western Himalayas. PloS one, 2015. 10(8): p. e0133823.

Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line

Year 2019, Volume: 49 Issue: 1, 25 - 32, 02.05.2019

Abstract

DOI: 10.26650/IstanbulJPharm.2019.418892


Callistemon citrinus is a member of
Myrtaceae family that thrives under different ecological conditions. The
leaves, flowers, stem backs and roots of the plant contain various
phytochemicals that are useful in   folk
medicine for different remedies such as antimicrobial, anti-nociceptive,
fungicide and anti-inflammatory purposes. In this study, we investigated the
cytotoxic effect of Callistemon citrinus leaf and flower methanolic extracts
against human Colo-205 Cell Line using real time cell analyzer device for
monitoring in time-dependent manner. To determine the mechanism of cytotoxicity
of the extracts, Western blotting assay was used for measuring evocation of Akt
pathway. Extracts were found to exert cytotoxic effect at a dose dependent
manner. IC50 values of leaves and flowers extract were 6.49 µg/mL and 5.22
µg/mL, respectively. At the early stages of the experiment, Akt pathway was
triggered at high extract concentrations. Although, high extract concentrations
showed proliferative effect at early stages, this effect reversed after 5 and 8
h resulting in low cell viability. Findings from this study therefore showed
that extracts of leaf and flower from Callistemon citrinus demonstrated
cytotoxic effect against Colo-205 but seems not to be related Akt signaling
pathway.

Cite this article as: Dokumacı AH, Fayemi
PO, Aycan Yerer B. (2019). Real time monitoring of cytotoxicity of Callistemon
citrinus against Colo-205 cell line. Istanbul J Pharm 49 (1): 25-32.

References

  • 1. Malvezzi, M., et al., European cancer mortality predictions for the year 2015: does lung cancer have the highest death rate in EU women. Ann Oncol, 2015. 26(4): p. 779-786.
  • 2. Harden, G.J., Flora of New South Wales. Vol. 4. 1990: UNSW Press.
  • 3. Oyedeji, O.O., et al., Chemical composition and antibacterial activity of the essential oils of Callistemon citrinus and Callistemon viminalis from South Africa. Molecules, 2009. 14(6): p. 1990-1998.
  • 4. Sudhakar, M., et al., Antinociceptive and anti-inflammatory effects of the standardized oil of Indian Callistemon lanceolatus leaves in experimental animals. East and Central African Journal of Pharmaceutical Sciences, 2004. 7(1): p. 10-15.
  • 5. Paluri, V., et al., Phytochemical composition and in vitro antimicrobial activity of methanolic extract of Callistemon lanceolatus DC. Int J Pharm Pharm Sci, 2012. 4(2): p. 699-2.
  • 6. Brodbeck, D., P. Cron, and B.A. Hemmings, A human protein kinase Bγ with regulatory phosphorylation sites in the activation loop and in the C-terminal hydrophobic domain. Journal of Biological Chemistry, 1999. 274(14): p. 9133-9136.
  • 7. Altomare, D.A., et al., Akt2 mRNA is highly expressed in embryonic brown fat and the AKT2 kinase is activated by insulin. Oncogene, 1998. 16(18): p. 2407-2411.
  • 8. Altomare, D.A., et al., Cloning, chromosomal localization and expression analysis of the mouse Akt2 oncogene. Oncogene, 1995. 11(6): p. 1055-1060.
  • 9. COFFER, P.J. and J.R. WOODGETT, Molecular cloning and characterisation of a novel putative protein‐serine kinase related to the cAMP‐dependent and protein kinase C families. European Journal of Biochemistry, 1991. 201(2): p. 475-481.
  • 10. Murthy, S., et al., Mapping of AKT3, encoding a member of the Akt/protein kinase B family, to human and rodent chromosomes by fluorescence in situ hybridization. Cytogenetic and Genome Research, 2000. 88(1-2): p. 38-40.
  • 11. Cheng, J.Q., et al., AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas. Proceedings of the National Academy of Sciences, 1992. 89(19): p. 9267-9271.
  • 12. Staal, S.P., et al., The AKT1 proto-oncogene maps to human chromosome 14, band q32. Genomics, 1988. 2(1): p. 96-98.
  • 13. Vivanco, I. and C.L. Sawyers, The phosphatidylinositol 3-kinase–AKT pathway in human cancer. Nature Reviews Cancer, 2002. 2(7): p. 489-501.
  • 14. Datta, S.R., A. Brunet, and M.E. Greenberg, Cellular survival: a play in three Akts. Genes & development, 1999. 13(22): p. 2905-2927.
  • 15. Blume-Jensen, P. and T. Hunter, Oncogenic kinase signalling. Nature, 2001. 411(6835): p. 355-365.
  • 16. Brognard, J., et al., Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer research, 2001. 61(10): p. 3986-3997.
  • 17. Kreisberg, J.I., et al., Phosphorylation of Akt (Ser473) is an excellent predictor of poor clinical outcome in prostate cancer. Cancer research, 2004. 64(15): p. 5232-5236.
  • 18. Grille, S.J., et al., The protein kinase Akt induces epithelial mesenchymal transition and promotes enhanced motility and invasiveness of squamous cell carcinoma lines. Cancer research, 2003. 63(9): p. 2172-2178.
  • 19. Sun, M., et al., Phosphatidylinositol-3-OH kinase (PI3K)/AKT2, activated in breast cancer, regulates and is induced by estrogen receptor α (ERα) via interaction between ERα and PI3K. Cancer research, 2001. 61(16): p. 5985-5991.
  • 20. Roy, H.K., et al., AKT proto-oncogene overexpression is an early event during sporadic colon carcinogenesis. Carcinogenesis, 2002. 23(1): p. 201-205.
  • 21. Itoh, N., et al., Phosphorylation of Akt/PKB is required for suppression of cancer cell apoptosis and tumor progression in human colorectal carcinoma. Cancer, 2002. 94(12): p. 3127-3134.
  • 22. Ke, N., et al., The xCELLigence system for real-time and label-free monitoring of cell viability. Mammalian Cell Viability: Methods and Protocols, 2011: p. 33-43.
  • 23. Bird, C. and S. Kirstein, Real-time, label-free monitoring of cellular invasion and migration with the xCELLigence system. Nature methods, 2009. 6(8).
  • 24. Kumar, D., et al., Chemical composition and in vitro cytotoxicity of essential oils from leaves and flowers of Callistemon citrinus from western Himalayas. PloS one, 2015. 10(8): p. e0133823.
There are 24 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Original Article
Authors

Alim Hüseyin Dokumacı 0000-0003-0035-1479

Peter Olutope Fayemi This is me

Mukerrem Betul Yerer This is me

Publication Date May 2, 2019
Submission Date April 26, 2018
Published in Issue Year 2019 Volume: 49 Issue: 1

Cite

APA Dokumacı, A. H., Fayemi, P. O., & Yerer, M. B. (2019). Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. İstanbul Journal of Pharmacy, 49(1), 25-32.
AMA Dokumacı AH, Fayemi PO, Yerer MB. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. iujp. May 2019;49(1):25-32.
Chicago Dokumacı, Alim Hüseyin, Peter Olutope Fayemi, and Mukerrem Betul Yerer. “Real Time Monitoring of Cytotoxicity of Callistemon Citrinus Against Colo-205 Cell Line”. İstanbul Journal of Pharmacy 49, no. 1 (May 2019): 25-32.
EndNote Dokumacı AH, Fayemi PO, Yerer MB (May 1, 2019) Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. İstanbul Journal of Pharmacy 49 1 25–32.
IEEE A. H. Dokumacı, P. O. Fayemi, and M. B. Yerer, “Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line”, iujp, vol. 49, no. 1, pp. 25–32, 2019.
ISNAD Dokumacı, Alim Hüseyin et al. “Real Time Monitoring of Cytotoxicity of Callistemon Citrinus Against Colo-205 Cell Line”. İstanbul Journal of Pharmacy 49/1 (May 2019), 25-32.
JAMA Dokumacı AH, Fayemi PO, Yerer MB. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. iujp. 2019;49:25–32.
MLA Dokumacı, Alim Hüseyin et al. “Real Time Monitoring of Cytotoxicity of Callistemon Citrinus Against Colo-205 Cell Line”. İstanbul Journal of Pharmacy, vol. 49, no. 1, 2019, pp. 25-32.
Vancouver Dokumacı AH, Fayemi PO, Yerer MB. Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line. iujp. 2019;49(1):25-32.