Real time monitoring of cytotoxicity of Callistemon citrinus against Colo-205 cell line

Year 2019, Volume: 49 Issue: 1, 25 - 32, 02.05.2019

Alim Hüseyin Dokumacı , Peter Olutope Fayemi Mukerrem Betul Yerer

Abstract

DOI: 10.26650/IstanbulJPharm.2019.418892

Callistemon citrinus is a member of
Myrtaceae family that thrives under different ecological conditions. The
leaves, flowers, stem backs and roots of the plant contain various
phytochemicals that are useful in   folk
medicine for different remedies such as antimicrobial, anti-nociceptive,
fungicide and anti-inflammatory purposes. In this study, we investigated the
cytotoxic effect of Callistemon citrinus leaf and flower methanolic extracts
against human Colo-205 Cell Line using real time cell analyzer device for
monitoring in time-dependent manner. To determine the mechanism of cytotoxicity
of the extracts, Western blotting assay was used for measuring evocation of Akt
pathway. Extracts were found to exert cytotoxic effect at a dose dependent
manner. IC50 values of leaves and flowers extract were 6.49 µg/mL and 5.22
µg/mL, respectively. At the early stages of the experiment, Akt pathway was
triggered at high extract concentrations. Although, high extract concentrations
showed proliferative effect at early stages, this effect reversed after 5 and 8
h resulting in low cell viability. Findings from this study therefore showed
that extracts of leaf and flower from Callistemon citrinus demonstrated
cytotoxic effect against Colo-205 but seems not to be related Akt signaling
pathway.

Cite this article as: Dokumacı AH, Fayemi
PO, Aycan Yerer B. (2019). Real time monitoring of cytotoxicity of Callistemon
citrinus against Colo-205 cell line. Istanbul J Pharm 49 (1): 25-32.

Keywords

Callistemon citrinus, Akt/p-Akt, colon cancer, xCELLigence

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