Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts

Year 2019, Volume: 49 Issue: 1, 7 - 13, 02.05.2019

Turgut Taşkın , Gizem Bulut

Abstract

DOI: 10.26650/IstanbulJPharm.2019.18012

The methanol extracts from different parts
of Rheum ribes were subjected to qualitative and quantitative phytochemical and
in vitro biological activity (antioxidant, anti-urease, anticholinesterase).
Qualitative phytochemical tests were performed using standard analysis methods
and these studies revealed the presence of phenolics and tannins. Following
this, a quantitative determination of total phenolics and tannins contents was
carried out. The antioxidant activity of the extracts were assayed using DPPH,
FRAP, TEAC/ABTS and CUPRAC techniques. In addition, the anti-urease and
anticholinesterase activity of the extracts were examined using indophenol and
Ellman methods, respectively. In this study, it was determined that the
macerated flowers extract contained higher total phenolic and tannins contents
than the other extracts. According to the results obtained from the antioxidant
experiment, the macerated extract of flowers showed the strongest ABTS.+
scavenging and ferric reducing antioxidant power activity. The macerated leaves
and Soxhlet radix extracts exhibited the strongest DPPH. scavenging and cupric
reducing antioxidant activity, respectively. The young shoots extracts obtained
using the Soxhlet methods showed the highest anticholinesterase activity. All
extracts obtained from different parts of the plant were found to have very low
anti-urease activity when compared to the 
anti-urease activity of standard compound. Therefore, methanol extracts
from plant’s flowers, leaves and young shoots can be used as a natural
antioxidant and anticholinesterase agent respectively, for the pharmaceutical
and food industry in the future.

Cite this article as: Taşkın T, Bulut G
(2019). Qualitative and quantitative phytochemical analysis and in-vitro
biological activity of Rheum ribes L. different parts. Istanbul J Pharm 49 (1):
7-13.

Keywords

Rheum ribes, antioxidant, anti-uresae, anticholinesterase, phytochemical analysis

References

• Amin M, Anwar F, Naz F, Mehmood T, Saari N (2013). Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules 18: 2135-2149. Amiri N, Shafaghat A, Salimi F (2015). Screening of the essential oil, hexane extract, chemical composition, antioxidant activity, and antimicrobial activity of the flower Rheum ribes L. from Iran. J Essent Oil Bear Pl 18: 1108-1115. Andiç S, Tunçtürk Y, Ocak E, Köse S (2009). Some chemical characteristics of edible wild rhubarb species (Rheum ribes L.) Res. J Agric Biol Sci 5: 973-977. Baskar R, Shrisakthi S, Sathyapriya B, Shyampriya R, Nithya R, Poongodi P (2011). Antioxidant potential of peel extracts of banana varieties (Musa sapientum). Food Nutr Sci 2: 1128-1133. Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239: 70-76. Bulut G, Biçer M, Tuzlacı E (2016). The folk medicinal plants of Yüksekova (Hakkâri-Turkey). J Fac Pharm Istanbul 46: 115-124. Buzzini P, Arapitsas P, Goretti M, Branda E, Turchetti B, Pinelli P, Leri F, Romani A (2008). Antimicrobial and antiviral activity of hydrolysable tannins. Mini Rev Med Chem 8: 1179-1187. Davis PH, Cullen J (1967). The Flora of Turkey, Vol 2, Edinburgh university press, Edinburgh, pp. 268-269. Ellman GL, Courtney KD, Andress V, Featherstone RM (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7: 88-95. Gholamhoseinian A, Moradi MN, Sharifi-far F (2009). Screening the methanol extracts of some Iranian plants for acetylcholinesterase inhibitory activity. Res Pharm Sci 4: 105-112. Ghous T, Akhtar K, Nasim FUH, Choudhry MA (2010). Screening of selected medicinal plants for urease inhibitory activity. Biol Med 2: 64-69. Nabati F, Mojab F, Habibi-Rezaei M, Bagherzadeh K, Amanlou M, Yousefi B (2012). Large scale screening of commonly used Iranian traditional medicinal plants against urease activity. Daru 20: 72. Ozsoy N, Can A, Yanardağ R, Akev N (2008). Antioxidant activity of Smilax excels L. leaf extracts. Food Chem 110: 571-583. Öztürk M, Öztürk FA, Duru ME, Topçu G (2007). Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chem 103: 623-630. Polat R, Cakilcioglu U, Ulusan MD, Paksoy MY (2015). Survey of wild food plants for human consumption in Elazığ (Turkey). IJTK 1: 69-75. Re R, Pellegrını N, Proteggente A, Pannala A, Yang M, Evans CR (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 26: 1231-1237. Shafaghat A, Amiri N, Salimi F (2014). Screening of flowers essential oil and hexane extract of Rheum ribes L. from Iran- Chemical composition, antioxidant and antimicrobial activities. JPHS 2: 115-123. Shahi MMN, Rad AHE, Shahi NN, Amin MRB (2016). Study of antioxidant activity and free radical scavengıng power of Rheum ribes flower extract. J Fundam Appl Sci 8: 1164-1174. Sharma V, Agarwal A (2015). Physicochemical and antioxidant assays of methanol and hydromethanol extract of ariel parts of Indigofera tinctoria Linn. Indian J Pharm Sci 77: 729-734. Sindhu R, Kumar P, Kumar J, Kumar A, Arora S (2010). Investıgatıons into the anti-ulcer activity of Rheum rıbes Linn leaves extracts. Int J Pharm Pharm Sci l2: 90-93. Taskin D, Alkaya BD, Dölen E (2017). HPLC-DAD/ESI-Q-TOF LC/MS Analysis of major phenolic compounds in flowers, leaves and stems of Achillea grandifolia and evaluation of their individual antioxidant properties. Chiang Mai J Sci 44: 1-12. Tosun F, Kızıla, AÇ (2003). Anthraquinones and flavonoids from Rheum ribes. J Fac Pharm 32: 31-35. Trease GE, Evans WC (2002). Pharmacognosy. 15th Ed. London: Saunders Publishers; pp. 391-393. Vijay DT, Rajendra SB (2014). Estimation of total phenol, tannin, alkaloid and flavonoid in Hibiscus tiliaceus L. wood extracts. research and review. J. Pharmacog Phytochem 2: 41-47. Zahedi M, Hojjati MR, Fathpour H, Rabiei Z, Alibabaei Z, Basim A (2015). Effect of Rheum ribes hydro-alcoholic extract on memory impairments in rat model of Alzheimer’s disease. Iran J Pharm Res 14: 1197-1206. Wei F, Jinglou C, Yaling C, Yongfang L, Liming C, Lei P (2010). Antioxidant, free radical scavenging, anti-inflammatoryand hepatoprotective potential of the extract from Parathelypteris nipponica (Franch.et Sav.) Ching. J Ethnopharmacol 130: 521-528.