Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts

Year 2019, Volume: 49 Issue: 1, 7 - 13, 02.05.2019

Turgut Taşkın Gizem Bulut

Abstract

DOI: 10.26650/IstanbulJPharm.2019.18012

The methanol extracts from different parts
of Rheum ribes were subjected to qualitative and quantitative phytochemical and
in vitro biological activity (antioxidant, anti-urease, anticholinesterase).
Qualitative phytochemical tests were performed using standard analysis methods
and these studies revealed the presence of phenolics and tannins. Following
this, a quantitative determination of total phenolics and tannins contents was
carried out. The antioxidant activity of the extracts were assayed using DPPH,
FRAP, TEAC/ABTS and CUPRAC techniques. In addition, the anti-urease and
anticholinesterase activity of the extracts were examined using indophenol and
Ellman methods, respectively. In this study, it was determined that the
macerated flowers extract contained higher total phenolic and tannins contents
than the other extracts. According to the results obtained from the antioxidant
experiment, the macerated extract of flowers showed the strongest ABTS.+
scavenging and ferric reducing antioxidant power activity. The macerated leaves
and Soxhlet radix extracts exhibited the strongest DPPH. scavenging and cupric
reducing antioxidant activity, respectively. The young shoots extracts obtained
using the Soxhlet methods showed the highest anticholinesterase activity. All
extracts obtained from different parts of the plant were found to have very low
anti-urease activity when compared to the 
anti-urease activity of standard compound. Therefore, methanol extracts
from plant’s flowers, leaves and young shoots can be used as a natural
antioxidant and anticholinesterase agent respectively, for the pharmaceutical
and food industry in the future.

Cite this article as: Taşkın T, Bulut G
(2019). Qualitative and quantitative phytochemical analysis and in-vitro
biological activity of Rheum ribes L. different parts. Istanbul J Pharm 49 (1):
7-13.

Keywords

Rheum ribes, antioxidant, anti-uresae, anticholinesterase, phytochemical analysis

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