The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver

Year 2019, Volume: 49 Issue: 3, 167 - 172, 01.12.2019

Yağmur Emre Arıcan , Ecem Fatma Karaman Sibel Özden

Abstract

Acetamiprid, which is a neonicotinoid insecticide, is used to control leafy vegetables, fruiting vegetables, fir seeds, citrus fruits,
pome fruits, grapes, cotton and ornamental plants and absorbent insects on flowers. The present study aim to evaluate global
DNA methylation and gene expression of DNA methylation related enzymes in liver and brain tissues of male Sprague-Dawley
rats after a 90-day subchronic exposure to acetamiprid at low doses of 12.5, 25 and 35 mg/kg body weight (b.w.). Global DNA
methylation resulted in a significant decrease in the levels of 5-methylcytosine (5-mC%) at the doses of 25 and 35 mg/kg b.w.
in the liver and 35 mg/kg b.w. in the brain compared to the vehicle control group. Consistently, expression of DNA methyltransferase enzymes decreased at doses of 12.5, 25 and 35 mg/kg b.w. in liver and 35 mg/kg b.w. in brain. It has been suggested that
non-genotoxic (epigenetic) mechanisms may be involved in the toxicity of acetamiprid and further investigations are needed
to elucidate the epigenetic effects of neonicotinoid insecticides.

Cite this article as: Arıcan YE, Karaman EF, Özden S (2019). The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver. Istanbul J Pharm 49 (3): 167-172.

Keywords

Acetamiprid, DNA methylation, Sprague-Dawley rats, liver, brain

References

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