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The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study

Year 2021, Volume: 51 Issue: 1, 35 - 41, 30.04.2021

Abstract

Background and Aims: The study was conducted to determine the optimal concentration of water-soluble snakehead fish dry extract (SFDE) in nanoemulsion and the amount of albumin required to penetrate the skin in order to accelerate the wound healing process. Methods: The snakehead fish (SF) was extracted using an atomizer while the nanoemulsion basis was optimized using oleic acid, Tween 80, and propylene glycol. The developed SFDE in nanoemulsion was characterized based on droplet size, PDI, and zeta potential. The ability of the mixture to penetrate the snakeskin was tested using Franz diffusion cells. The effectiveness of the nanoemulsion was evaluated by dividing the rabbits used for experiment into 6 treatment groups including SFDE F1 0.25%, F2 0.5%, F3 1%, F4 SF 2% cream, F5 nanoemulsion basis, and F6 no treatment. Results: The SFDE nanoemulsion produced a particle size of 147.5 nm with acceptable PDI (0.23) and zeta potential (+13.38 mV). The most effective SFDE to accelerate the healing of open wounds in rabbits was a concentration of 1%, which was found to have dried and closed the wound on the 3rd day. Conclusion: The permeation study and the effectiveness test showed the 1% SFDE nanoemulsion is the best concentration in accelerating the wound healing process and ensuring the highest albumin penetration into the skin.

References

  • • Alves, M. P., Scarrone, A. L., Santos, M., Pohlmann, A. R., & Guterren S. S. (2007). Human skin penetration and distribution of nimesulide from hydrophilic gels. International Journal of Pharmaceutics, 314(1-2), 215-220.
  • • Barot, B. S., Parejiya, P. B., Patel, H. K, Mehta D. M., & Shelat, P. K. (2012). Microemulsion-based antifungal gel delivery to nail for the treatment of onychomycosis: Formulation, optimization, and efficacy studies. Drug Delivery and Translational Research, 2(6), 463–476.
  • • Bernard, P. B. (2012). Modern Aspects of Emulsion Science, Emulsions- Recent Advances in Understanding. UK: Royal Science of Chemistry.
  • • Brandelero, R. P. H., Yamashita, F., & Grossmann, M. V. E. (2010). The effect of surfactant Tween 80 on the hydrophilic water vapor permeation, and the mechanical properties of cassava starch and poly (butylenes adipate-co-terephtalate) (pbat) blend films. Carbohydrate Polymers, 82, 1102-1109.
  • • Ceve, G. (2004). Lipid vesicles and other colloids as drug carriers on the skin. Advanced Drug Delivery Review, 56(5), 675-711.
  • • Chuesiang, P., Siripatrawan, U., Sanguandeekul, R., McLandsborough, L., & McClements, D. J. (2018). Optimization of cinnamon oil nanoemulsions using phase inversion temperature method: Impact of oil phase composition and surfactant concentration. Journal of Colloid and Interface Science, 514, 208-216.
  • • Devarajan, V., & Ravichandran, V. (2011). Nanoemulsions: As modified drug delivery tool. International Journal of Comprehensive Pharmacy, 2, 1-5.
  • • Hadian, Z., Sahari, M. A., & Moghimi, H. R. (2014). Formulation, characterization and optimization of liposomes containing EPA and DHA; A methodology approach. Iranian Journal of Pharmaceutical Research, 13(2), 393-404.
  • • Kong, M., Chen, X. G., Kweon, D. K., & Park, H. J. (2011). Investigation on skin hyaluronic acid based on nanoemulsion as transdermal carrier. Carbohydrate Polymers, 86(2), 837-843.
  • • Kumar, M., Bishnoi, R. S., Shukla, A. K., & Jain, P. (2019). Techniques for formulation of nanoemulsion drug delivery system: A review. Preventive Nutrition and Food Science, 24(3), 225-234.
  • • Lala, R., & Awari, N. (2014). Nanoemulsion-based gel formulations of COX-2 inhibitors for enhanced efficacy in inflammatory conditions. Applied Nanoscience, 4, 143–151.
  • • Laxmi, M., Bhardwaj, A., Mehta, S., & Mehta, A. (2015). Development and characterization of nanoemulsion as carrier for the enhancement of bioavailability of artemether. Artificial Cells Nanomedicine and Biotechnology, 43(5), 334-344.
  • • Lovelyn, C., & Attama, A. A. (2011). Current state of nanoemulsions in drug delivery. Journal of Biomaterials and Nanobiotechnology, 2(5), 626-639.
  • • Madene, A., Jacquot, M., Scher, J., & Desobry, S. (2006). Flavour encapsulation and controlled release-a review. International Journal of Food Science and Technology, 41, 1-21.
  • • Mansyur. (2010, July 15). Analysis of snakehead fish dry extract. Indonesian Institute of Sciences Biotechnology Research Center. Retrieved from https://worldwidescience.org/topicpages/s/ snakehead+fish+channa.html
  • • Maryanto, A. (2004, June 18). The impact of albumin serum on length of postoperative wound healing process, Faculty of Medicine, University of Gadjah Mada. Retrieved from http://etd.repository. ugm.ac.id/home/detail_pencarian/25247
  • • Mou, D., Chen, H., Du, D., Mao, C., Wan, J., Xu, H., & Yang, X. (2008). Hydrogel thickened nanoemulsion system for topical delivery of lipophilic drugs. International Journal of Pharmaceutics, 353(1-2), 270-276.
  • • Mustafa, A., Widodo, A., & Kristianto, Y. (2012). Albumin and zinc content of snakehead fish extract and its role in health. International. Journal of Science and Technology, 1, 1-8. • Natalia, M. (2012). The stability and antibacterial activity test of black cumin oil (nigella sativa l.) nano-emulsion gel (nanoemulgel). (Master’s thesis). Retrieved from http://lib.ui.ac.id/ file?file=digital/20309121-S43091-Uji%20stabilitas.pdf
  • • Ngawhirunpat, T., Panomsuk, S., Opanasopit, P., Rojanata, T., & Hatanaka, T. (2006). Comparison of the percutaneous absorption of hydrophilic and lipophilic compounds in shed snake skin and human skin. Pharmazie, 61(4), 331-335.
  • • Rehman, F. U., Shah, K. U., Shah, S. U., Khan, I. U., Khan, G. M., & Khan, A. (2017). From nanoemulsions to self-nanoemulsions, with recent advances in self-nanoemulsifying drug delivery systems (SNEDDS). Expert Opinion on Drug Delivery, 14(11), 1325-1340.
  • • Shah, P., Bhalodia, D., & Shelat, P. (2011). Nanoemulsion : A pharmaceutical review. Systematic Reviews in Pharmacy, 1, 24-32.
  • • Singh, R., & Lillard, J. W. (2014). Nanoparticle-based targeted drug delivery. Experimental Molecular and Pathology, 86(3), 215-223.
  • • Suprayitno, E. (2003). Snakehead Fish (Ophiocephalus striatus) albumin as functional food to overcome future nutrition problems. Faculty of Fisheries, Brawijaya University, 5(3), 32-36. • Swarbrick, J. (2007). Encyclopedia of pharmaceutical technology. New York: Informa Healthcare USA Press, pp 1548-1565.
  • • Tungadi, R. (2011). The acceleration of wound healing of snakehead fish cream towards rabbit’s skin wound histopathologically. Indonesian Pharmaceutical Journal, 9, 91-97.
  • • Tungadi, R., & Hasan, A. M. (2016). The effect of penetrant enhancer combination towards the diffusion rate of snakehead fish (Ophiocephalus striatus) cream in vitro and vivo. International Journal of PharmTech Research, 9(6), 508-13.
  • • Tungadi, R., Moo, D. R., & Mozin, W. R. (2017). Characterization and physical stability evaluation of snakehead fish (Ophiocephalus striatus) powder nanoemulsion. International Journal of Pharmaceutical Sciences and Research, 8(6), 2720-4.
  • • Tungadi, R., Susanty, W., Wicita, P., & Pido, E. (2018). Transdermal delivery of snakehead fish (Ophiocephalus striatus) nanoemulgel containing hydrophobic powder for burn wound. Pharmaceutical Sciences, 24(4), 313-323.
  • • Tungadi, R. (2019). Potential of snakehead fish (Ophiocephalus striatus) in accelerating wound healing. Universal Journal of Pharmaceutical Research, 4(5), 40-44.
  • • Tungadi, R., Abdulkadir, W., Ischak, N. I., & Rahim, B. R. (2019). Liposomal formulation of snakehead fish (Ophiocephalus striatus) powder and toxicity study in zebrafish (Danio rerio) model. Pharmaceutical Sciences, 25(2), 145-153.
  • • Tungadi, R., & Wicita, P. (2020). Formulation, optimization, and characterization of snakehead fish (Ophiocephalus striatus) powder nanoemulgel. Brazilian Journal of Pharmaceutical Sciences, 56, 1-8.
  • • Ulandari, A., Kurniawan, D., & Putri, A. S. (2011). Potential of snakehead fish protein in preventing kwashiorkor in toddlers in Jambi Province. Faculty of Medicine, Jambi University. Retrieved from https://adoc.pub/potensi-protein-ikan-gabus-dalam-mencegah- kwashiorkor-pada-b.html
  • • Vemula, V. R., Lagishetty, V., & Lingala, S. (2010). Solubility enhancement techniques. International Journal of Pharmaceutical Science Review and Research, 5(1), 41–51.
  • • Winterhalter, M., & Lasic, D. D. (2013). Liposome stability and formation: experimental parameters and theories on the size distribution. Chemistry and Physics of Lipids, 64, 35-37.
  • • Zhang, L., Zhang, F., Fan, Z., Liu, B., & Meng, X. (2019). DHA and EPA nanoemulsion prepared by the low-energy emulsification method: process factors influencing droplet size physicochemical stability. Food Research International, 121(7), 359-366.
  • • Zhu, W., Guo, C., Yu, A., Gao, Y., Cao, F., & Zhai, G. (2009). Microemulsion- based Hydrogel Formulation of penciclovir for topical delivery. International Journal of Pharmaceutics, 378(1-2), 152-158.
There are 35 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences, Health Care Administration
Journal Section Original Article
Authors

Robert Tungadi This is me 0000-0003-2141-2402

Widysusanti Abdulkadir This is me 0000-0002-8975-134X

Munafri Tahir This is me 0000-0002-9351-2843

Publication Date April 30, 2021
Submission Date May 10, 2020
Published in Issue Year 2021 Volume: 51 Issue: 1

Cite

APA Tungadi, R., Abdulkadir, W., & Tahir, M. (2021). The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. İstanbul Journal of Pharmacy, 51(1), 35-41.
AMA Tungadi R, Abdulkadir W, Tahir M. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. iujp. April 2021;51(1):35-41.
Chicago Tungadi, Robert, Widysusanti Abdulkadir, and Munafri Tahir. “The Formulation and Characterization of Water-Soluble Snakehead Fish (Ophiocephalus Striatus) Dry Extract in Nanoemulsion Using Permeation and in Vivo Study”. İstanbul Journal of Pharmacy 51, no. 1 (April 2021): 35-41.
EndNote Tungadi R, Abdulkadir W, Tahir M (April 1, 2021) The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. İstanbul Journal of Pharmacy 51 1 35–41.
IEEE R. Tungadi, W. Abdulkadir, and M. Tahir, “The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study”, iujp, vol. 51, no. 1, pp. 35–41, 2021.
ISNAD Tungadi, Robert et al. “The Formulation and Characterization of Water-Soluble Snakehead Fish (Ophiocephalus Striatus) Dry Extract in Nanoemulsion Using Permeation and in Vivo Study”. İstanbul Journal of Pharmacy 51/1 (April 2021), 35-41.
JAMA Tungadi R, Abdulkadir W, Tahir M. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. iujp. 2021;51:35–41.
MLA Tungadi, Robert et al. “The Formulation and Characterization of Water-Soluble Snakehead Fish (Ophiocephalus Striatus) Dry Extract in Nanoemulsion Using Permeation and in Vivo Study”. İstanbul Journal of Pharmacy, vol. 51, no. 1, 2021, pp. 35-41.
Vancouver Tungadi R, Abdulkadir W, Tahir M. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. iujp. 2021;51(1):35-41.