Research Article
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Year 2021, Volume: 51 Issue: 3, 313 - 318, 29.12.2021

Abstract

References

  • • Abels, C., & Soeberdt, M. (2019). Can we teach old drugs new tricks?-Repurposing of neuropharmacological drugs for inflammatory skin diseases. Experimental Dermatology, 28(9), 1002-1009. http://dx.doi.org.10.1111/exd.13987
  • • Bainbridge, P. (2013). Wound healing and the role of fibroblasts. Journal of Wound Care, 22(8), 407-408, 410-412. http://dx.doi. org.10.12968/jowc.2013.22.8.407
  • • Chun, D. I., Kim, S., Kim, J., Yang, H. J., Kim, J. H., . . . Won, S. H. (2019). Epidemiology and Burden of Diabetic Foot Ulcer and Peripheral Arterial Disease in Korea. Journal of Clinical Medicine, 8(5). http:// dx.doi.org.10.3390/jcm8050748
  • • Everett, E., & Mathioudakis, N. (2018). Update on management of diabetic foot ulcers. Annals of New York Academic Sciences, 1411(1), 153-165. http://dx.doi.org.10.1111/nyas.13569
  • • Frohlich, E., & Wahl, R. (2019). The forgotten effects of thyrotropin- releasing hormone: Metabolic functions and medical applications. Frontiers in Neuroendocrinolgy, 52, 29-43. http://dx.doi. org.10.1016/j.yfrne.2018.06.006
  • • Gaspar, E., Hardenbicker, C., Bodo, E., Wenzel, B., Ramot, Y., Paus, R. (2010). Thyrotropin releasing hormone (TRH): a new player in human hair-growth control. FASEB Journal, 24(2), 393-403. http:// dx.doi.org.10.1096/fj.08-126417
  • • Gouin, J. P., & Kiecolt-Glaser, J. K. (2011). The impact of psychological stress on wound healing: methods and mechanisms. Immunology and Allergy Clinics of North America, 31(1), 81-93. http:// dx.doi.org.10.1016/j.iac.2010.09.010
  • • Gupta, C., & Tikoo, K. (2013). High glucose and insulin differentially modulates proliferation in MCF-7 and MDA-MB-231 cells. Journal of Molecular Endocrinology, 51(1), 119-129. http://dx.doi. org.10.1530/JME-13-0062
  • • Hahm, G., Glaser, J. J., & Elster, E. A. (2011). Biomarkers to predict wound healing: the future of complex war wound management. Plastic and Reconstrative Surgery, 127 (Suppl 1), 21S-26S. http:// dx.doi.org.10.1097/PRS.0b013e3181fbe291
  • • Kassem, R., Liberty, Z., Babaev, M., Trau, H., & Cohen, O. (2012). Harnessing the skin-thyroid connection for wound healing: a prospective controlled trial in guinea pigs. Clinical and Experimental Dermatology, 37(8), 850-856. http://dx.doi.org.10.1111/j.1365- 2230.2012.04456.x
  • • Kaykhaei, M. A., Shahraki, M. R., Mohammadi, M, Moshtaghian, B., Zandkarimi, M. (2016). The Effects of Topical Application of Thyroid Hormone (Liothyronine, T3) on Skin Wounds in Diabetic Wistar Rats. Zahedan Journal of Research in Medical Science., 18(4), e6449. http://dx.doi.org.10.17795/zjrms-6449
  • • Leguina-Ruzzi, A., & Valderas, J. P. (2017). BLT2 expression improves skin integrity and protects from alterations caused by hyperglycemia in type 2 diabetes. Dermatoendocrinolgy, 9(1), e1267078. http://dx.doi.org.10.1080/19381980.2016.1267078
  • • Lennox, J., & Johnston, I. D. (1973). The effect of thyroid status on nitrogen balance and the rate of wound healing after injury in rats. British Journal of Surgery, 60(4), 309. Retrieved from https:// www.ncbi.nlm.nih.gov/pubmed/4700245
  • • Lerman, O. Z., Galiano, R. D., Armour, M., Levine, J. P., & Gurtner, G. C. (2003). Cellular dysfunction in the diabetic fibroblast: impairment in migration, vascular endothelial growth factor production, and response to hypoxia. Americal Journal of Pathology, 162(1), 303- 312. http://dx.doi.org.10.1016/S0002-9440(10)63821-7
  • • Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. http://dx.doi.org.10.1038/ nprot.2007.30
  • • Mehregan, A. H., & Zamick, P. (1974). The effect of triiodothyronine in healing of deep dermal burns and marginal scars of skin grafts. A histologic study. Journal of Cutanaeous Pathology, 1(3), 113-116. http://dx.doi.org.10.1111/j.1600-0560.1974.tb00201.x
  • • Meier, N. T., Haslam, I. S., Pattwell, D. M., Zhang, G. Y., Emelianov, V., . . . Paus, R. (2013). Thyrotropin-releasing hormone (TRH) promotes wound re-epithelialization in frog and human skin. PLoS One, 8(9), e73596. http://dx.doi.org.10.1371/journal.pone.0073596
  • • Morais, C., Westhuyzen, J., Pat, B., Gobe, G., & Healy, H. (2005). High ambient glucose is effect neutral on cell death and proliferation in human proximal tubular epithelial cells. American Journal of Physiology Renal Physiology, 289(2), F401-409. http://dx.doi. org.10.1152/ajprenal.00408.2004
  • • Nie, C., Yang, D., Liu, N., Dong, D., Xu, J., & Zhang, J. (2014). Thyrotropin- releasing hormone and its analogs accelerate wound healing. Journal of Surgical Research, 189(2), 359-365. http://dx.doi. org.10.1016/j.jss.2014.03.004
  • • Ohnstedt, E., Lofton Tomenius, H., Vagesjo, E., & Phillipson, M. (2019). The discovery and development of topical medicines for wound healing. Expert Opinion in Drug Discovery, 14(5), 485-497. http://dx.doi.org.10.1080/17460441.2019.1588879
  • • Pan, F., Guo, R., Cheng, W., Chai, L., Wang, W., . . . Li, S. (2015). High glucose inhibits ClC-2 chloride channels and attenuates cell migration of rat keratinocytes. Drug Design Development and Therapy, 9, 4779-4791. http://dx.doi.org.10.2147/DDDT.S84628
  • • Pastar, I., Stojadinovic, O., & Tomic-Canic, M. (2008). Role of keratinocytes in healing of chronic wounds. Surgical Technology International, 17, 105-112. Retrieved from https://www.ncbi.nlm.nih. gov/pubmed/18802889
  • • Pitz, H. S., Pereira, A., Blasius, M. B., Voytena, A. P., Affonso, R. C., . . . Maraschin, M. (2016). In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract. Oxidative Medicine and Cellular Longevity, 2016, 3403586. http://dx.doi.org.10.1155/2016/3403586
  • • Qi, L., Ahmadi, A. R., Huang, J., Chen, M., Pan, B., . . . Sun, Z. (2020). Major Improvement in Wound Healing Through Pharmacologic Mobilization of Stem Cells in Severely Diabetic Rats. Diabetes, 69(4), 699-712. http://dx.doi.org.10.2337/db19-0907
  • • Safer, J. D., Crawford, T. M., & Holick, M. F. (2005). Topical thyroid hormone accelerates wound healing in mice. Endocrinology, 146(10), 4425-4430. http://dx.doi.org.10.1210/en.2005-0192
  • • Schreml, S.,& Berneburg, M. (2017). The global burden of diabetic wounds. British Journal of Dermatology, 176(4), 845-846. http:// dx.doi.org.10.1111/bjd.15254
  • • Slominski, A., Wortsman, J., Kohn, L., Ain, K. B., Venkataraman, G. M., . . . Tobin, D. J. (2002). Expression of hypothalamic-pituitarythyroid axis related genes in the human skin. Journal of Investigative Dermatology, 119(6), 1449-1455. http://dx.doi.org.10.1046/ j.1523-1747.2002.19617.x
  • • Tarameshloo, M., Norouzian, M., Zarein-Dolab, S., Dadpay, M., & Gazor, R. (2012). A comparative study of the effects of topical application of Aloe vera, thyroid hormone and silver sulfadiazine on skin wounds in Wistar rats. Laboratory Animal Research, 28(1), 17- 21. http://dx.doi.org.10.5625/lar.2012.28.1.17
  • • Ueck, C., Volksdorf, T., Houdek, P., Vidal, Y. S. S., Sehner, S., . . . Brandner, J. M. (2017). Comparison of In-Vitro and Ex-Vivo Wound Healing Assays for the Investigation of Diabetic Wound Healing and Demonstration of a Beneficial Effect of a Triterpene Extract. PLoS One, 12(1), e0169028. http://dx.doi.org.10.1371/journal.pone.0169028
  • • Vidali, S., Knuever, J., Lerchner, J., Giesen, M., Biro, T., . . . Paus, R. (2014). Hypothalamic-pituitary-thyroid axis hormones stimulate mitochondrial function and biogenesis in human hair follicles. Journal of Investigative Dermatolgy, 134(1), 33-42. http://dx.doi. org.10.1038/jid.2013.286
  • • Werner, S., Krieg, T., & Smola, H. (2007). Keratinocyte-fibroblast interactions in wound healing. Journal of Investigative Dermatolgy, 127(5), 998-1008. http://dx.doi.org.10.1038/sj.jid.5700786
  • • WHO. (2020). Diabetes, Key facts. Retrieved from https://www. who.int/news-room/fact-sheets/detail/diabetes
  • • Zamick, P., & Mehregan, A. H. (1973). Effect of 1-tri iodothyronine on marginal scars of skin grafted burns in rats. Plastic and Reconstructive Surgery, 51(1), 71-75. http://dx.doi.org.10.1097/00006534- 197301000-00013
  • • Zhang, G. Y., Langan, E. A., Meier, N. T., Funk, W., Siemers, F., & Paus, R. (2019). Thyroxine (T4) may promote re-epithelialization and angiogenesis in wounded human skin ex vivo. PLoS One, 14(3), e0212659. http://dx.doi.org.10.1371/journal.pone.0212659

Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line

Year 2021, Volume: 51 Issue: 3, 313 - 318, 29.12.2021

Abstract

Background and Aims: It has been suggested that thyrotropin-releasing hormone (TRH) may have functions beyond its fundamental regulating function. Previous studies have demonstrated that TRH promotes wound healing. We aimed to perform an in vitro study in fibroblasts to assess the role of TRH in wounds that frequently occur in diabetes. Thus, we investigated the effects of TRH in wound healing both under normoglycemic and hyperglycemic-conditions. Methods: L929 mouse fibroblast cell line was used in the experiments. The cell viability was confirmed with XTT. Then the scratch migration assay was used for assessing the wound healing. TRH was added to both control and hyperglycemia groups at 100 nM after the scratch was created. The wound areas were measured after 24 and 48 hours after the scratch. Results: TRH and/or hyperglycemia did not affect the cellular activity after 48 hours. TRH reduced the wound areas (42.6%) compared to the control (52.2%) after 24 hours. In the hyperglycemia group the wound area was 64.3% and 61.0% of initial area at the 24th and 48th hours respectively. TRH incubation reduced these wound areas to 55.2% and 47.1% of initial areas. Conclusion: TRH treatment accelerated wound healing in hyperglycemia, which indicates the positive effects of TRH in wounds, may occur in diabetes.

References

  • • Abels, C., & Soeberdt, M. (2019). Can we teach old drugs new tricks?-Repurposing of neuropharmacological drugs for inflammatory skin diseases. Experimental Dermatology, 28(9), 1002-1009. http://dx.doi.org.10.1111/exd.13987
  • • Bainbridge, P. (2013). Wound healing and the role of fibroblasts. Journal of Wound Care, 22(8), 407-408, 410-412. http://dx.doi. org.10.12968/jowc.2013.22.8.407
  • • Chun, D. I., Kim, S., Kim, J., Yang, H. J., Kim, J. H., . . . Won, S. H. (2019). Epidemiology and Burden of Diabetic Foot Ulcer and Peripheral Arterial Disease in Korea. Journal of Clinical Medicine, 8(5). http:// dx.doi.org.10.3390/jcm8050748
  • • Everett, E., & Mathioudakis, N. (2018). Update on management of diabetic foot ulcers. Annals of New York Academic Sciences, 1411(1), 153-165. http://dx.doi.org.10.1111/nyas.13569
  • • Frohlich, E., & Wahl, R. (2019). The forgotten effects of thyrotropin- releasing hormone: Metabolic functions and medical applications. Frontiers in Neuroendocrinolgy, 52, 29-43. http://dx.doi. org.10.1016/j.yfrne.2018.06.006
  • • Gaspar, E., Hardenbicker, C., Bodo, E., Wenzel, B., Ramot, Y., Paus, R. (2010). Thyrotropin releasing hormone (TRH): a new player in human hair-growth control. FASEB Journal, 24(2), 393-403. http:// dx.doi.org.10.1096/fj.08-126417
  • • Gouin, J. P., & Kiecolt-Glaser, J. K. (2011). The impact of psychological stress on wound healing: methods and mechanisms. Immunology and Allergy Clinics of North America, 31(1), 81-93. http:// dx.doi.org.10.1016/j.iac.2010.09.010
  • • Gupta, C., & Tikoo, K. (2013). High glucose and insulin differentially modulates proliferation in MCF-7 and MDA-MB-231 cells. Journal of Molecular Endocrinology, 51(1), 119-129. http://dx.doi. org.10.1530/JME-13-0062
  • • Hahm, G., Glaser, J. J., & Elster, E. A. (2011). Biomarkers to predict wound healing: the future of complex war wound management. Plastic and Reconstrative Surgery, 127 (Suppl 1), 21S-26S. http:// dx.doi.org.10.1097/PRS.0b013e3181fbe291
  • • Kassem, R., Liberty, Z., Babaev, M., Trau, H., & Cohen, O. (2012). Harnessing the skin-thyroid connection for wound healing: a prospective controlled trial in guinea pigs. Clinical and Experimental Dermatology, 37(8), 850-856. http://dx.doi.org.10.1111/j.1365- 2230.2012.04456.x
  • • Kaykhaei, M. A., Shahraki, M. R., Mohammadi, M, Moshtaghian, B., Zandkarimi, M. (2016). The Effects of Topical Application of Thyroid Hormone (Liothyronine, T3) on Skin Wounds in Diabetic Wistar Rats. Zahedan Journal of Research in Medical Science., 18(4), e6449. http://dx.doi.org.10.17795/zjrms-6449
  • • Leguina-Ruzzi, A., & Valderas, J. P. (2017). BLT2 expression improves skin integrity and protects from alterations caused by hyperglycemia in type 2 diabetes. Dermatoendocrinolgy, 9(1), e1267078. http://dx.doi.org.10.1080/19381980.2016.1267078
  • • Lennox, J., & Johnston, I. D. (1973). The effect of thyroid status on nitrogen balance and the rate of wound healing after injury in rats. British Journal of Surgery, 60(4), 309. Retrieved from https:// www.ncbi.nlm.nih.gov/pubmed/4700245
  • • Lerman, O. Z., Galiano, R. D., Armour, M., Levine, J. P., & Gurtner, G. C. (2003). Cellular dysfunction in the diabetic fibroblast: impairment in migration, vascular endothelial growth factor production, and response to hypoxia. Americal Journal of Pathology, 162(1), 303- 312. http://dx.doi.org.10.1016/S0002-9440(10)63821-7
  • • Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. http://dx.doi.org.10.1038/ nprot.2007.30
  • • Mehregan, A. H., & Zamick, P. (1974). The effect of triiodothyronine in healing of deep dermal burns and marginal scars of skin grafts. A histologic study. Journal of Cutanaeous Pathology, 1(3), 113-116. http://dx.doi.org.10.1111/j.1600-0560.1974.tb00201.x
  • • Meier, N. T., Haslam, I. S., Pattwell, D. M., Zhang, G. Y., Emelianov, V., . . . Paus, R. (2013). Thyrotropin-releasing hormone (TRH) promotes wound re-epithelialization in frog and human skin. PLoS One, 8(9), e73596. http://dx.doi.org.10.1371/journal.pone.0073596
  • • Morais, C., Westhuyzen, J., Pat, B., Gobe, G., & Healy, H. (2005). High ambient glucose is effect neutral on cell death and proliferation in human proximal tubular epithelial cells. American Journal of Physiology Renal Physiology, 289(2), F401-409. http://dx.doi. org.10.1152/ajprenal.00408.2004
  • • Nie, C., Yang, D., Liu, N., Dong, D., Xu, J., & Zhang, J. (2014). Thyrotropin- releasing hormone and its analogs accelerate wound healing. Journal of Surgical Research, 189(2), 359-365. http://dx.doi. org.10.1016/j.jss.2014.03.004
  • • Ohnstedt, E., Lofton Tomenius, H., Vagesjo, E., & Phillipson, M. (2019). The discovery and development of topical medicines for wound healing. Expert Opinion in Drug Discovery, 14(5), 485-497. http://dx.doi.org.10.1080/17460441.2019.1588879
  • • Pan, F., Guo, R., Cheng, W., Chai, L., Wang, W., . . . Li, S. (2015). High glucose inhibits ClC-2 chloride channels and attenuates cell migration of rat keratinocytes. Drug Design Development and Therapy, 9, 4779-4791. http://dx.doi.org.10.2147/DDDT.S84628
  • • Pastar, I., Stojadinovic, O., & Tomic-Canic, M. (2008). Role of keratinocytes in healing of chronic wounds. Surgical Technology International, 17, 105-112. Retrieved from https://www.ncbi.nlm.nih. gov/pubmed/18802889
  • • Pitz, H. S., Pereira, A., Blasius, M. B., Voytena, A. P., Affonso, R. C., . . . Maraschin, M. (2016). In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract. Oxidative Medicine and Cellular Longevity, 2016, 3403586. http://dx.doi.org.10.1155/2016/3403586
  • • Qi, L., Ahmadi, A. R., Huang, J., Chen, M., Pan, B., . . . Sun, Z. (2020). Major Improvement in Wound Healing Through Pharmacologic Mobilization of Stem Cells in Severely Diabetic Rats. Diabetes, 69(4), 699-712. http://dx.doi.org.10.2337/db19-0907
  • • Safer, J. D., Crawford, T. M., & Holick, M. F. (2005). Topical thyroid hormone accelerates wound healing in mice. Endocrinology, 146(10), 4425-4430. http://dx.doi.org.10.1210/en.2005-0192
  • • Schreml, S.,& Berneburg, M. (2017). The global burden of diabetic wounds. British Journal of Dermatology, 176(4), 845-846. http:// dx.doi.org.10.1111/bjd.15254
  • • Slominski, A., Wortsman, J., Kohn, L., Ain, K. B., Venkataraman, G. M., . . . Tobin, D. J. (2002). Expression of hypothalamic-pituitarythyroid axis related genes in the human skin. Journal of Investigative Dermatology, 119(6), 1449-1455. http://dx.doi.org.10.1046/ j.1523-1747.2002.19617.x
  • • Tarameshloo, M., Norouzian, M., Zarein-Dolab, S., Dadpay, M., & Gazor, R. (2012). A comparative study of the effects of topical application of Aloe vera, thyroid hormone and silver sulfadiazine on skin wounds in Wistar rats. Laboratory Animal Research, 28(1), 17- 21. http://dx.doi.org.10.5625/lar.2012.28.1.17
  • • Ueck, C., Volksdorf, T., Houdek, P., Vidal, Y. S. S., Sehner, S., . . . Brandner, J. M. (2017). Comparison of In-Vitro and Ex-Vivo Wound Healing Assays for the Investigation of Diabetic Wound Healing and Demonstration of a Beneficial Effect of a Triterpene Extract. PLoS One, 12(1), e0169028. http://dx.doi.org.10.1371/journal.pone.0169028
  • • Vidali, S., Knuever, J., Lerchner, J., Giesen, M., Biro, T., . . . Paus, R. (2014). Hypothalamic-pituitary-thyroid axis hormones stimulate mitochondrial function and biogenesis in human hair follicles. Journal of Investigative Dermatolgy, 134(1), 33-42. http://dx.doi. org.10.1038/jid.2013.286
  • • Werner, S., Krieg, T., & Smola, H. (2007). Keratinocyte-fibroblast interactions in wound healing. Journal of Investigative Dermatolgy, 127(5), 998-1008. http://dx.doi.org.10.1038/sj.jid.5700786
  • • WHO. (2020). Diabetes, Key facts. Retrieved from https://www. who.int/news-room/fact-sheets/detail/diabetes
  • • Zamick, P., & Mehregan, A. H. (1973). Effect of 1-tri iodothyronine on marginal scars of skin grafted burns in rats. Plastic and Reconstructive Surgery, 51(1), 71-75. http://dx.doi.org.10.1097/00006534- 197301000-00013
  • • Zhang, G. Y., Langan, E. A., Meier, N. T., Funk, W., Siemers, F., & Paus, R. (2019). Thyroxine (T4) may promote re-epithelialization and angiogenesis in wounded human skin ex vivo. PLoS One, 14(3), e0212659. http://dx.doi.org.10.1371/journal.pone.0212659
There are 34 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Original Article
Authors

Merve Denizaltı 0000-0001-5363-606X

İnci Kazkayası This is me

Gokcen Tellı 0000-0003-0028-6769

Publication Date December 29, 2021
Submission Date May 5, 2021
Published in Issue Year 2021 Volume: 51 Issue: 3

Cite

APA Denizaltı, M., Kazkayası, İ., & Tellı, G. (2021). Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line. İstanbul Journal of Pharmacy, 51(3), 313-318.
AMA Denizaltı M, Kazkayası İ, Tellı G. Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line. iujp. December 2021;51(3):313-318.
Chicago Denizaltı, Merve, İnci Kazkayası, and Gokcen Tellı. “Thyrotropine Releasing Hormone Accelerates Wound Healing in Hyperglycemia Inducted Mouse Fibroblast Cell Line”. İstanbul Journal of Pharmacy 51, no. 3 (December 2021): 313-18.
EndNote Denizaltı M, Kazkayası İ, Tellı G (December 1, 2021) Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line. İstanbul Journal of Pharmacy 51 3 313–318.
IEEE M. Denizaltı, İ. Kazkayası, and G. Tellı, “Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line”, iujp, vol. 51, no. 3, pp. 313–318, 2021.
ISNAD Denizaltı, Merve et al. “Thyrotropine Releasing Hormone Accelerates Wound Healing in Hyperglycemia Inducted Mouse Fibroblast Cell Line”. İstanbul Journal of Pharmacy 51/3 (December 2021), 313-318.
JAMA Denizaltı M, Kazkayası İ, Tellı G. Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line. iujp. 2021;51:313–318.
MLA Denizaltı, Merve et al. “Thyrotropine Releasing Hormone Accelerates Wound Healing in Hyperglycemia Inducted Mouse Fibroblast Cell Line”. İstanbul Journal of Pharmacy, vol. 51, no. 3, 2021, pp. 313-8.
Vancouver Denizaltı M, Kazkayası İ, Tellı G. Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line. iujp. 2021;51(3):313-8.