Research Article
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The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenols

Year 2024, Volume: 54 Issue: 1, 40 - 48, 30.04.2024
https://doi.org/10.26650/IstanbulJPharm.2024.1299245

Abstract

Background and Aims: Standard treatment regimens for fibrocystic breast disease (FBD) do not provide a permanent cure and have undesirable side effects. This study aims to investigate the therapeutic potential of different honey and propolis, as well as some important polyphenols, on breast epithelial cells (MCF-10A).

Materials and Methods: The effects of five honey, two propolis extracts and seven polyphenol samples on the cell viability were assessed the WST-1 assay. Content analysis of the propolis samples was performed using high performance liquid chromatography (HPLC).

Results: Chestnut and cedar honey had antiproliferative effects on MCF-10A cells at all doses (1-10 μg/mL), as well as pine honey at the highest dose. However, multifloral honey had no similar effect. Chinese propolis had significant antiproliferative effects on MCF-10A cells at doses of 50-250 μg/mL and on the human periodontal ligament (hPDL) control cells at a dose of 5 μg/mL. Türkiye propolis only had an antiproliferative effect on MCF-10A cells at the highest dose (p = 0.0013). Higher levels of ferulic acid, kaempferol, caffeic acid, pinocembrin and quercetin were detected in Türkiye propolis, while Chinese propolis was rich in pinostrobin. Ferulic acid, pinostrobin and galangin showed antiproliferative properties on MCF-10A cells (p < 0.0001), whereas the remaining four polyphenols had no significant effect on cell viability (p > 0.05).

Conclusion: The findings of the study highlight the antiproliferative effects of pinostrobin, ferulic acid and galangin on MCF-10A cells and has also confirmed the antiproliferative effects of honey and propolis samples to be due to their polyphenolic properties. Therefore, this study suggests that polyphenolic substances may have both preventive and therapeutic potential in FBD.

Supporting Institution

Istanbul University Scientific Research Projects Unit.

Project Number

29818 - 29383

Thanks

We thank Prof. Dr. Ahmet C. Gören and Istanbul University Scientific Research Projects Unit.

References

  • Abbas Momtazi-borojeni, A., Behbahani, M., & Sadeghi-Aliabadi, H. (2013). Antiproliferative activity and apoptosis induction of crude extract and fractions of Avicennia marina. Iranian Journal of Basic Medical Sciences, 16(11), 1203. google scholar
  • Abutayeh, A. (2014). Die Wirkung der kommerziell erworbe-nen Flavonoide (Quercetin, Kaempferol und Chrysin) auf die malignen Mammakarzinomzellen (MCF7 und BT20) und die benignen veranderten Mammazellen (MCF10a und MCF12a)(Östrogenrezeptor positiv und negativ) in vitro (Doc-toral dissertation, Universitatsmedizin Rostock). google scholar
  • Ahn, M. R., Kumazawa, S., Hamasaka, T., Bang , K. S., & Nakayama, T. (2004). Antioxidant activity and constituents of propolis collected in various areas of Korea. Journal of Agricultural and Food Chemistry, 52(24), 7286-7292. https://doi.org/10.1021/jf048726s. google scholar
  • Aiello, F., Armentano, B., Polera, N., Carullo, G., Loizzo, M. R., Bonesi, M., ... & Tundis, R. (2017). From vegetable waste to new agents for potential health applications: Antioxidant properties and effects of extracts, fractions and pinocembrin from Glycyrrhiza glabra L. aerial parts on viability of five human cancer cell lines. Journal of Agricultural and Food Chemistry, 65(36), 7944-7954. https://doi.org/10.1021/acs.jafc.7b03045 google scholar
  • Alipour, S., Rastad, H., Saberi, A., Faiz, F., Maleki-Hajiagha, A., & Abedi, M. (2021). Metformin in the management of fibro-cystic breast disease: a placebo-controlled randomized clinical trial. journal of Faculty of Pharmacy, Tehran University of Med-ical Sciences, 29(2), 389-396. https://doi.org/10.1007/s40199-021-00424-6 google scholar
  • Aryappalli, P., Al-Qubaisi, S. S., Attoub, S., George, J. A., Arafat, K., Ramadi, K. B., ... & Al-Ramadi, B. K. (2017). The IL-6/STAT3 signaling pathway is an early target of manuka honey-induced suppression of human breast cancer cells. Frontiers in Oncology, 7, 167. https://doi.org/10.3389/fonc.2017.00167 google scholar
  • Bino, R. J., De Vos, C. R., Lieberman, M., Hall, R. D., Bovy, A., Jonker, H. H., & Levin, I. (2005). The light-hyperresponsive high pigment-2dg mutation of tomato: alter-ations in the fruit metabolome. New Phytologist, 166(2), 427-438. https://doi.org/10.1111/j.1469-8137.2005.01362.x google scholar
  • Brkic, M., Vujovic, S., Ivovic, M., Gajic, M. T., Marina, L., Ivanisevic, M. F., & Franic, D. (2018). The role of E2/P ratio in the etiology of fibrocystic breast disease, mastalgia and mastodynia. Acta Clinica Croatica, 57(4), 756. https://doi: 10.20471/acc.2018.57.04.18 google scholar
  • Fauzi, A. N., Norazmi, M. N., & Yaacob, N. S. (2011). Tu-alang honey induces apoptosis and disrupts the mitochon-drial membrane potential of human breast and cervical can-cer cell lines. Food and Chemical Toxicology, 49(4), 871-878. https://doi.org/10.1016/j.fct.2010.12.010 google scholar
  • Gateley, C. A., Maddox, P. R., Pritchard, G. A., Sheridan, W., Harrison, B. J., Pye, J. K., ... & Mansel, R. E. (1992). Plasma fatty acid profiles in benign breast disorders. Journal of British Surgery, 79(5), 407-409. https://doi.org/10.1002/bjs.1800790511 google scholar
  • Gateley, C. A., Miers, M., Mansel, R. E., & Hughes, L. E. (1992). Drug treatments for mastalgia: 17 years experience in the Cardiff Mastalgia Clinic. Journal ofthe Royal Society of Medicine, 85(1), 12-15. https://doi.org/10.1177/014107689208500105 google scholar
  • Godazandeh, G., Ala, S., Motlaq, T. M., Sahebnasagh, A., & Bazi, A. (2021). The comparison of the effect of flaxseed oil and vitamin E on mastalgia and nodularity of breast fibrocystic: a random-ized double-blind clinical trial. Journal of Pharmaceutical Health Care and Sciences, 7(1), 1-8. https://doi.org/10.1186/s40780-020-00186-4 google scholar
  • Gopalani, S. V., Janitz, A. E., Martinez, S. A., Gutman, P., Khan, S., & Campbell, J. E. (2020). Trends in Cancer Incidence among American Indians and Alaska natives and non-hispanic whites in the United States, 1999-2015. Epidemiology, 31(2), 205-213. https://doi.org/10.1097/EDE.0000000000001140 google scholar
  • Greenblatt, R. B., Samaras C, Vasquez, J. M., Nezhat C. (1982). Fibro-cystic disease of the breast. Clinical Obstetrics and Gynecology, 25(2), 365-71. google scholar
  • Hung, H. (2004). Inhibition of estrogen receptor alpha expression and function in MCF-7 cells by kaempferol. Journal of Cellular Physiology, 198(2), 197-208. https://doi.org/10.1002/jcp.10398 google scholar
  • Irgebay, Z., Yeszhan, B., Sen, B., Tuleukhanov, S., Brooks, A. D., Sensenig, R., & Orynbayeva, Z. (2017). Dana-zol alters mitochondria metabolism of fibrocystic breast Mcf10A cells. Breast (Edinburgh, Scotland), 35, 55-62. https://doi.org/10.1016/j.breast.2017.06.007 google scholar
  • Jaganathan, S. K., & Mandal, M. (2009). Antiproliferative ef-fects of honey and of its polyphenols: A review. Jour-nal of Biomedicine and Biotechnology, 2009, 830616. https://doi.org/10.1155/2009/830616 google scholar
  • Jones, A. A., & Gehler, S. (2020). Acacetin and pinostrobin inhibit malignant breast epithelial cell adhesion and focal adhesion forma-tion to attenuate cell migration. Integrative Cancer Therapies, 19, 1534735420918945. https://doi.org/10.1177/1534735420918945 google scholar
  • Khan, M. K., & Dangles, O. (2014). A comprehensive re-view on flavanones, the major citrus polyphenols. Jour-nal of Food Composition and Analysis, 33(1), 85-104. https://doi.org/10.1016/j.jfca.2013.11.004 google scholar
  • Lusby, P. E., Coombes, A. L., & Wilkinson, J. M. (2005). Bactericidal activity of different honeys against pathogenic bacteria. Archives of Medical Research, 36(5), 464-467. https://doi.org/10.1016/j.arcmed.2005.03.038 google scholar
  • Mandal, M. D., & Mandal, S. (2011). Honey: its medicinal prop-erty and antibacterial activity. Asian Pacific Journal of Tropi-cal Biomedicine, 1(2), 154-160. https://doi.org/10.1016/S2221-1691(11)60016-6 google scholar
  • Mansel, R., Goyal, A., Nestour, E. L., Masini-Eteve, V., O’Connell, K., & Afimoxifene (4-OHT) Breast Pain Research Group. (2007). A phase II trial of Afimoxifene (4-hydroxytamoxifen gel) for cycli-cal mastalgia in premenopausal women. Breast Cancer Research and Treatment, 106, 389-397. https://doi.org/10.1007/s10549-007-9507-x google scholar
  • McMullen, E. R., Zoumberos, N. A., & Kleer, C. G. (2019). Metaplas-tic breast carcinoma: update on histopathology and molecular al-terations. Archives of Pathology & Laboratory Medicine, 143(12), 1492-1496. https://doi.org/10.5858/arpa.2019-0396-RA google scholar
  • Mohamed, W. A. S., Ismail, N. Z., Omar, E. A., Abdul Samad, N., Adam, S. K., & Mohamad, S. (2020). GC-MS evaluation, antioxidant content, and cytotoxic activity of propolis extract from Peninsular Malaysian stingless bees, Tetrigona apicalis. Evidence-Based Complementary and Alternative Medicine, 2020. https://doi.org/10.1155/2020/8895262 google scholar
  • Omene, C., Kalac, M., Wu, J., Marchi, E., Frenkel, K., & O’Connor, O. A. (2013). Propolis and its active component, caffeic acid phenethyl ester (CAPE), modulate breast cancer therapeutic tar-gets via an epigenetically mediated mechanism of action. Journal of Cancer Science & Therapy, 5(10), 334. google scholar
  • Pham, H. N. T., Sakoff, J. A., Vuong, Q. V., Bowyer, M. C., & Scarlett, C. J. (2018). Comparative cytotoxic activity between kaempferol and gallic acid against various cancer cell lines. Data in brief, 21, 1033-1036. https://doi.org/10.1016/j.dib.2018.10.121 google scholar
  • Sasaki, J., Geletzke, A., Kass, R. B., Klimberg, V. S., Copeland III, E. M., & Bland, K. I. (2018). Etiology and management of benign breast disease. The Breast, 79-92. https://doi.org/10.1016/B978-0-323-35955-9.00005-2 google scholar
  • Serafim, T. L., Carvalho, F. S., Marques, M. P., Calheiros, R., Silva, T., Garrido, J., ... & Oliveira, P. J. (2011). Lipophilic caffeic and fer-ulic acid derivatives presenting cytotoxicity against human breast cancer cells. Chemical Research in Toxicology, 24(5), 763-774. https://doi.org/10.1021/tx200126r google scholar
  • Seyhan, M. F., Yılmaz, E., Timirci-Kahraman, Ö., Saygılı, N., Kısake-sen, H. İ., Eronat, A. P., ... & Öztürk, O. (2017). Anatolian honey is not only sweet but can also protect from breast cancer: Elixir for women from artemis to present. IUBMB life, 69(9), 677-688. https://doi.org/10.1002/iub.1652 google scholar
  • Seyhan, M. F., Yılmaz, E., Timirci-Kahraman, Ö., Saygılı, N., Kısake-sen, H. İ., Gazioğlu, S., ... & Öztürk, O. (2019). Different propolis samples, phenolic content, and breast cancer cell lines: Variable cytotoxicity ranging from ineffective to potent. IUBMB life, 71(5), 619-631. https://doi.org/10.1002/iub.1996 google scholar
  • Silici, S., & Kutluca, S. (2005). Chemical composition and antibac-terial activity of propolis collected by three different races of honeybees in the same region. Journal of Ethnopharmacology, 99(1), 69-73. https://doi.org/10.1016/j.jep.2005.01.046 google scholar
  • Song, W., Yan, C. Y., Zhou, Q. Q., & Zhen, L. L. (2017). Galangin potentiates human breast cancer to apoptosis induced by TRAIL through activating AMPK. Biomedicine & Pharmacotherapy, 89, 845-856. https://doi.org/10.1016/j.biopha.2017.01.062 google scholar
  • Tu, C., Ren, X., He, J., Zhang, C., Chen, R., Wang, W., & Li, Z. (2019). The value of LncRNA BCAR4 as a prognostic biomarker on clinical outcomes in human cancers. Journal of Cancer, 10(24), 5992. https://doi.org/10.7150/jca.35113 google scholar
  • Verma, B., Hucl, P., & Chibbar, R. N. (2009). Phenolic acid composi-tion and antioxidant capacity of acid and alkali hydrolysed wheat bran fractions. Food Chemistry, 116(4), 947-954. google scholar
  • Vorherr, H. (1986). Fibrocystic breast disease: Pathophysiology, pathomorphology, clinical picture, and management. Ameri-can Journal of Obstetrics and Gynecology 154(1):161-179. https://doi.org/10.1016/0002-9378(86)90421-7. google scholar
  • Yadav, P., Sharma, A., Singh, L., & Gupta, R. (2020). Management of fibrocystic breast disease: A comprehensive review. Journal of Advanced Scientific Research, 11(04), 30-37. google scholar
  • Yang, B., Huang, J., Xiang, T., Yin, X., Luo, X., Huang, J., ... & Ren, G. (2014). Chrysin inhibits metastatic potential of hu-man triple-negative breast cancer cells by modulating matrix metalloproteinase-10, epithelial to mesenchymal transition, and PI3K/Akt signaling pathway. Journal of Applied Toxicology, 34(1), 105-112. https://doi.org/10.1002/jat.2941 google scholar
Year 2024, Volume: 54 Issue: 1, 40 - 48, 30.04.2024
https://doi.org/10.26650/IstanbulJPharm.2024.1299245

Abstract

Project Number

29818 - 29383

References

  • Abbas Momtazi-borojeni, A., Behbahani, M., & Sadeghi-Aliabadi, H. (2013). Antiproliferative activity and apoptosis induction of crude extract and fractions of Avicennia marina. Iranian Journal of Basic Medical Sciences, 16(11), 1203. google scholar
  • Abutayeh, A. (2014). Die Wirkung der kommerziell erworbe-nen Flavonoide (Quercetin, Kaempferol und Chrysin) auf die malignen Mammakarzinomzellen (MCF7 und BT20) und die benignen veranderten Mammazellen (MCF10a und MCF12a)(Östrogenrezeptor positiv und negativ) in vitro (Doc-toral dissertation, Universitatsmedizin Rostock). google scholar
  • Ahn, M. R., Kumazawa, S., Hamasaka, T., Bang , K. S., & Nakayama, T. (2004). Antioxidant activity and constituents of propolis collected in various areas of Korea. Journal of Agricultural and Food Chemistry, 52(24), 7286-7292. https://doi.org/10.1021/jf048726s. google scholar
  • Aiello, F., Armentano, B., Polera, N., Carullo, G., Loizzo, M. R., Bonesi, M., ... & Tundis, R. (2017). From vegetable waste to new agents for potential health applications: Antioxidant properties and effects of extracts, fractions and pinocembrin from Glycyrrhiza glabra L. aerial parts on viability of five human cancer cell lines. Journal of Agricultural and Food Chemistry, 65(36), 7944-7954. https://doi.org/10.1021/acs.jafc.7b03045 google scholar
  • Alipour, S., Rastad, H., Saberi, A., Faiz, F., Maleki-Hajiagha, A., & Abedi, M. (2021). Metformin in the management of fibro-cystic breast disease: a placebo-controlled randomized clinical trial. journal of Faculty of Pharmacy, Tehran University of Med-ical Sciences, 29(2), 389-396. https://doi.org/10.1007/s40199-021-00424-6 google scholar
  • Aryappalli, P., Al-Qubaisi, S. S., Attoub, S., George, J. A., Arafat, K., Ramadi, K. B., ... & Al-Ramadi, B. K. (2017). The IL-6/STAT3 signaling pathway is an early target of manuka honey-induced suppression of human breast cancer cells. Frontiers in Oncology, 7, 167. https://doi.org/10.3389/fonc.2017.00167 google scholar
  • Bino, R. J., De Vos, C. R., Lieberman, M., Hall, R. D., Bovy, A., Jonker, H. H., & Levin, I. (2005). The light-hyperresponsive high pigment-2dg mutation of tomato: alter-ations in the fruit metabolome. New Phytologist, 166(2), 427-438. https://doi.org/10.1111/j.1469-8137.2005.01362.x google scholar
  • Brkic, M., Vujovic, S., Ivovic, M., Gajic, M. T., Marina, L., Ivanisevic, M. F., & Franic, D. (2018). The role of E2/P ratio in the etiology of fibrocystic breast disease, mastalgia and mastodynia. Acta Clinica Croatica, 57(4), 756. https://doi: 10.20471/acc.2018.57.04.18 google scholar
  • Fauzi, A. N., Norazmi, M. N., & Yaacob, N. S. (2011). Tu-alang honey induces apoptosis and disrupts the mitochon-drial membrane potential of human breast and cervical can-cer cell lines. Food and Chemical Toxicology, 49(4), 871-878. https://doi.org/10.1016/j.fct.2010.12.010 google scholar
  • Gateley, C. A., Maddox, P. R., Pritchard, G. A., Sheridan, W., Harrison, B. J., Pye, J. K., ... & Mansel, R. E. (1992). Plasma fatty acid profiles in benign breast disorders. Journal of British Surgery, 79(5), 407-409. https://doi.org/10.1002/bjs.1800790511 google scholar
  • Gateley, C. A., Miers, M., Mansel, R. E., & Hughes, L. E. (1992). Drug treatments for mastalgia: 17 years experience in the Cardiff Mastalgia Clinic. Journal ofthe Royal Society of Medicine, 85(1), 12-15. https://doi.org/10.1177/014107689208500105 google scholar
  • Godazandeh, G., Ala, S., Motlaq, T. M., Sahebnasagh, A., & Bazi, A. (2021). The comparison of the effect of flaxseed oil and vitamin E on mastalgia and nodularity of breast fibrocystic: a random-ized double-blind clinical trial. Journal of Pharmaceutical Health Care and Sciences, 7(1), 1-8. https://doi.org/10.1186/s40780-020-00186-4 google scholar
  • Gopalani, S. V., Janitz, A. E., Martinez, S. A., Gutman, P., Khan, S., & Campbell, J. E. (2020). Trends in Cancer Incidence among American Indians and Alaska natives and non-hispanic whites in the United States, 1999-2015. Epidemiology, 31(2), 205-213. https://doi.org/10.1097/EDE.0000000000001140 google scholar
  • Greenblatt, R. B., Samaras C, Vasquez, J. M., Nezhat C. (1982). Fibro-cystic disease of the breast. Clinical Obstetrics and Gynecology, 25(2), 365-71. google scholar
  • Hung, H. (2004). Inhibition of estrogen receptor alpha expression and function in MCF-7 cells by kaempferol. Journal of Cellular Physiology, 198(2), 197-208. https://doi.org/10.1002/jcp.10398 google scholar
  • Irgebay, Z., Yeszhan, B., Sen, B., Tuleukhanov, S., Brooks, A. D., Sensenig, R., & Orynbayeva, Z. (2017). Dana-zol alters mitochondria metabolism of fibrocystic breast Mcf10A cells. Breast (Edinburgh, Scotland), 35, 55-62. https://doi.org/10.1016/j.breast.2017.06.007 google scholar
  • Jaganathan, S. K., & Mandal, M. (2009). Antiproliferative ef-fects of honey and of its polyphenols: A review. Jour-nal of Biomedicine and Biotechnology, 2009, 830616. https://doi.org/10.1155/2009/830616 google scholar
  • Jones, A. A., & Gehler, S. (2020). Acacetin and pinostrobin inhibit malignant breast epithelial cell adhesion and focal adhesion forma-tion to attenuate cell migration. Integrative Cancer Therapies, 19, 1534735420918945. https://doi.org/10.1177/1534735420918945 google scholar
  • Khan, M. K., & Dangles, O. (2014). A comprehensive re-view on flavanones, the major citrus polyphenols. Jour-nal of Food Composition and Analysis, 33(1), 85-104. https://doi.org/10.1016/j.jfca.2013.11.004 google scholar
  • Lusby, P. E., Coombes, A. L., & Wilkinson, J. M. (2005). Bactericidal activity of different honeys against pathogenic bacteria. Archives of Medical Research, 36(5), 464-467. https://doi.org/10.1016/j.arcmed.2005.03.038 google scholar
  • Mandal, M. D., & Mandal, S. (2011). Honey: its medicinal prop-erty and antibacterial activity. Asian Pacific Journal of Tropi-cal Biomedicine, 1(2), 154-160. https://doi.org/10.1016/S2221-1691(11)60016-6 google scholar
  • Mansel, R., Goyal, A., Nestour, E. L., Masini-Eteve, V., O’Connell, K., & Afimoxifene (4-OHT) Breast Pain Research Group. (2007). A phase II trial of Afimoxifene (4-hydroxytamoxifen gel) for cycli-cal mastalgia in premenopausal women. Breast Cancer Research and Treatment, 106, 389-397. https://doi.org/10.1007/s10549-007-9507-x google scholar
  • McMullen, E. R., Zoumberos, N. A., & Kleer, C. G. (2019). Metaplas-tic breast carcinoma: update on histopathology and molecular al-terations. Archives of Pathology & Laboratory Medicine, 143(12), 1492-1496. https://doi.org/10.5858/arpa.2019-0396-RA google scholar
  • Mohamed, W. A. S., Ismail, N. Z., Omar, E. A., Abdul Samad, N., Adam, S. K., & Mohamad, S. (2020). GC-MS evaluation, antioxidant content, and cytotoxic activity of propolis extract from Peninsular Malaysian stingless bees, Tetrigona apicalis. Evidence-Based Complementary and Alternative Medicine, 2020. https://doi.org/10.1155/2020/8895262 google scholar
  • Omene, C., Kalac, M., Wu, J., Marchi, E., Frenkel, K., & O’Connor, O. A. (2013). Propolis and its active component, caffeic acid phenethyl ester (CAPE), modulate breast cancer therapeutic tar-gets via an epigenetically mediated mechanism of action. Journal of Cancer Science & Therapy, 5(10), 334. google scholar
  • Pham, H. N. T., Sakoff, J. A., Vuong, Q. V., Bowyer, M. C., & Scarlett, C. J. (2018). Comparative cytotoxic activity between kaempferol and gallic acid against various cancer cell lines. Data in brief, 21, 1033-1036. https://doi.org/10.1016/j.dib.2018.10.121 google scholar
  • Sasaki, J., Geletzke, A., Kass, R. B., Klimberg, V. S., Copeland III, E. M., & Bland, K. I. (2018). Etiology and management of benign breast disease. The Breast, 79-92. https://doi.org/10.1016/B978-0-323-35955-9.00005-2 google scholar
  • Serafim, T. L., Carvalho, F. S., Marques, M. P., Calheiros, R., Silva, T., Garrido, J., ... & Oliveira, P. J. (2011). Lipophilic caffeic and fer-ulic acid derivatives presenting cytotoxicity against human breast cancer cells. Chemical Research in Toxicology, 24(5), 763-774. https://doi.org/10.1021/tx200126r google scholar
  • Seyhan, M. F., Yılmaz, E., Timirci-Kahraman, Ö., Saygılı, N., Kısake-sen, H. İ., Eronat, A. P., ... & Öztürk, O. (2017). Anatolian honey is not only sweet but can also protect from breast cancer: Elixir for women from artemis to present. IUBMB life, 69(9), 677-688. https://doi.org/10.1002/iub.1652 google scholar
  • Seyhan, M. F., Yılmaz, E., Timirci-Kahraman, Ö., Saygılı, N., Kısake-sen, H. İ., Gazioğlu, S., ... & Öztürk, O. (2019). Different propolis samples, phenolic content, and breast cancer cell lines: Variable cytotoxicity ranging from ineffective to potent. IUBMB life, 71(5), 619-631. https://doi.org/10.1002/iub.1996 google scholar
  • Silici, S., & Kutluca, S. (2005). Chemical composition and antibac-terial activity of propolis collected by three different races of honeybees in the same region. Journal of Ethnopharmacology, 99(1), 69-73. https://doi.org/10.1016/j.jep.2005.01.046 google scholar
  • Song, W., Yan, C. Y., Zhou, Q. Q., & Zhen, L. L. (2017). Galangin potentiates human breast cancer to apoptosis induced by TRAIL through activating AMPK. Biomedicine & Pharmacotherapy, 89, 845-856. https://doi.org/10.1016/j.biopha.2017.01.062 google scholar
  • Tu, C., Ren, X., He, J., Zhang, C., Chen, R., Wang, W., & Li, Z. (2019). The value of LncRNA BCAR4 as a prognostic biomarker on clinical outcomes in human cancers. Journal of Cancer, 10(24), 5992. https://doi.org/10.7150/jca.35113 google scholar
  • Verma, B., Hucl, P., & Chibbar, R. N. (2009). Phenolic acid composi-tion and antioxidant capacity of acid and alkali hydrolysed wheat bran fractions. Food Chemistry, 116(4), 947-954. google scholar
  • Vorherr, H. (1986). Fibrocystic breast disease: Pathophysiology, pathomorphology, clinical picture, and management. Ameri-can Journal of Obstetrics and Gynecology 154(1):161-179. https://doi.org/10.1016/0002-9378(86)90421-7. google scholar
  • Yadav, P., Sharma, A., Singh, L., & Gupta, R. (2020). Management of fibrocystic breast disease: A comprehensive review. Journal of Advanced Scientific Research, 11(04), 30-37. google scholar
  • Yang, B., Huang, J., Xiang, T., Yin, X., Luo, X., Huang, J., ... & Ren, G. (2014). Chrysin inhibits metastatic potential of hu-man triple-negative breast cancer cells by modulating matrix metalloproteinase-10, epithelial to mesenchymal transition, and PI3K/Akt signaling pathway. Journal of Applied Toxicology, 34(1), 105-112. https://doi.org/10.1002/jat.2941 google scholar
There are 37 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences, Health Care Administration
Journal Section Original Article
Authors

İnclay Çelik 0000-0003-2512-7756

Mehmet Fatih Seyhan 0000-0002-6218-0049

Ayşe Begüm Ceviz 0000-0002-3635-8421

Çağatay Aydoğan 0000-0001-7520-070X

Hülya Yılmaz Aydoğan 0000-0002-8837-6664

Oğuz Öztürk 0000-0002-2439-9269

Project Number 29818 - 29383
Publication Date April 30, 2024
Submission Date May 18, 2023
Published in Issue Year 2024 Volume: 54 Issue: 1

Cite

APA Çelik, İ., Seyhan, M. F., Ceviz, A. B., Aydoğan, Ç., et al. (2024). The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenols. İstanbul Journal of Pharmacy, 54(1), 40-48. https://doi.org/10.26650/IstanbulJPharm.2024.1299245
AMA Çelik İ, Seyhan MF, Ceviz AB, Aydoğan Ç, Yılmaz Aydoğan H, Öztürk O. The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenols. iujp. April 2024;54(1):40-48. doi:10.26650/IstanbulJPharm.2024.1299245
Chicago Çelik, İnclay, Mehmet Fatih Seyhan, Ayşe Begüm Ceviz, Çağatay Aydoğan, Hülya Yılmaz Aydoğan, and Oğuz Öztürk. “The Therapeutic Approach to Fibrocystic Breast Disease in the MCF-10A Cell Culture Model: Striking Efficacy of Polyphenols”. İstanbul Journal of Pharmacy 54, no. 1 (April 2024): 40-48. https://doi.org/10.26650/IstanbulJPharm.2024.1299245.
EndNote Çelik İ, Seyhan MF, Ceviz AB, Aydoğan Ç, Yılmaz Aydoğan H, Öztürk O (April 1, 2024) The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenols. İstanbul Journal of Pharmacy 54 1 40–48.
IEEE İ. Çelik, M. F. Seyhan, A. B. Ceviz, Ç. Aydoğan, H. Yılmaz Aydoğan, and O. Öztürk, “The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenols”, iujp, vol. 54, no. 1, pp. 40–48, 2024, doi: 10.26650/IstanbulJPharm.2024.1299245.
ISNAD Çelik, İnclay et al. “The Therapeutic Approach to Fibrocystic Breast Disease in the MCF-10A Cell Culture Model: Striking Efficacy of Polyphenols”. İstanbul Journal of Pharmacy 54/1 (April 2024), 40-48. https://doi.org/10.26650/IstanbulJPharm.2024.1299245.
JAMA Çelik İ, Seyhan MF, Ceviz AB, Aydoğan Ç, Yılmaz Aydoğan H, Öztürk O. The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenols. iujp. 2024;54:40–48.
MLA Çelik, İnclay et al. “The Therapeutic Approach to Fibrocystic Breast Disease in the MCF-10A Cell Culture Model: Striking Efficacy of Polyphenols”. İstanbul Journal of Pharmacy, vol. 54, no. 1, 2024, pp. 40-48, doi:10.26650/IstanbulJPharm.2024.1299245.
Vancouver Çelik İ, Seyhan MF, Ceviz AB, Aydoğan Ç, Yılmaz Aydoğan H, Öztürk O. The therapeutic approach to fibrocystic breast disease in the MCF-10A cell culture model: Striking efficacy of polyphenols. iujp. 2024;54(1):40-8.