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Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam.

Year 2024, Volume: 54 Issue: 2, 182 - 188, 26.08.2024
https://doi.org/10.26650/IstanbulJPharm.2024.1036392

Abstract

Background and Aims: Centaurea is a significant widespread genus in the Asteraceae family. Türkiye has 194 species, of which 118 are endemic. Free radicals can alter lipids, proteins, and DNA and trigger many diseases. However, a person with an insufficient immune system needs outside assistance to scavenge free radicals. Antioxidants protect the body from oxidative stress by scavenging free radicals. Fats are also very important because they contain fat-soluble vitamins, form lipoproteins with proteins, and affect health. Fatty acids serve as part of molecules in cells or alone as building blocks of cell membranes. They also promote energy and signaling molecules. Polyunsaturated fatty acids (PUFAs) have significant antioxidant activity. Studies are found showing PUFAs to be able to be used as a preservative in foods. These effects are due to phenolic hydroxyl groups. The aim of the current study is to evaluate the fatty acid profile of C. virgata to determine the fatty acid content and examine the antioxidant activities related to major fatty acids.

Methods: Gas chromatography Mass Spectrometry (GC/MS) analyses were carried out to determine the fatty acid content. Furthermore, the in vitro antioxidant activities of methanol extract were determined using DPPH (2, 2’-Diphenyl-1-picrylhydrazyl) and ABTS(2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging and the reducing power assay methods. The program IBM SPSS Statistics (ver. 25) was used to evaluate antioxidant activities.

Results: C. virgata’s major components were found to be palmitic acid (30.13%), oleic acid (9.17%), and linoleic acid (11.64%). The DPPH results indicated the IC50 value of the methanol extract to be 378.86 ± 0.60 μg/mL, while ABTS was found to be 19.38 ± 0.49 μg/mL. The results from the reducing power assay found the absorbance of ascorbic acid to be 0.55 at a 100 μg/mL concentration and it measure 0.12 in C. virgata.

Conclusion: According to the evaluation results, harmony is seen to exist between the groups regarding the activities

References

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  • Zengin, G., Aktümsek, A., Güler, G.Ö., Çakmak, Y.S.& Yıldıztugay, E. (2011). Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. subsp. hayekiana Wa-genitz. Records of Natural Products, 5 (2), 123-132. google scholar
  • Zengin, G., Çakmak, Y.S., Guler, G.O. & Aktumsek, A. (2010). In vitro antioxidant capacities and fatty acid compositions of three Cen-taurea species collected from Central Anatolia region of Turkey. Food Chemistry and Toxicology, 48, 2368-2341. [CrossRef] google scholar
  • Zengin, G., Guler, G.O., Cakmak, Y.S. & Aktumsek, A. (2011). An-tioxidant capacity and fatty acid profile of Centaurea kotschyi (Boiss.& Heldr.) Hayek var. persica (Boiss.) Wagenitz from Turkey. Grasas Y Acaites, 62 (1), 90- 95. [CrossRef] google scholar
  • Zengin, G., L-lorent-Martinez, E., Sinan, K.I., Yıldıztugay, E., Picot-Allain, C.&Mahomoodally, M.F. (2019a). Chemical profiling of Centaurea bornmuelleri Hausskn. aerial parts by HPLC-MS/MS and their pharmaceutical effects: From nature to novel perspec-tives. Journal of Pharmaceutical and Biomedical Analysis, 174, 406-413. [CrossRef] google scholar
  • Zengin, G., Zheleva-Dimitrovab, D., Gevrenovab, R., Aktumsek, A., Sinan, K.I. & Mahomood, M.F. (2019b). A comparative assess- google scholar
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  • Zengin, G., Zhelava-Dimitrova, D., Gevrenova, R., Nedialkov, P, Mo-can, A....& Ciric, A. (2018). Identification of phenolic compo- google scholar
  • nents via LC-MS analysis and biological activities of two Centau-rea species: Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia. Journal of Pharmaceutical and Biomedical Analysis, 149, 436- 441. [CrossRef] google scholar
Year 2024, Volume: 54 Issue: 2, 182 - 188, 26.08.2024
https://doi.org/10.26650/IstanbulJPharm.2024.1036392

Abstract

Supporting Institution

EGE ÜNİVERSİTESİ

References

  • Aktumsek, A., Zengin, G., Guler, G.O., Cakmak, Y.S. & Duran, A. (2011). Screening for in vitro antioxidant capacities and fatty acid profiles of five Centaurea L. species from Turkey flora. Food Chemistry and Toxicology, 49, 2914-2920. [CrossRef] google scholar
  • Aktumsek, A., Zengin, G., Guler, G.O., Cakmak, Y.S. & Duran, A. (2013). Assesment of the antioxidant potential and fatty acid com-position of four Centaurea L. taxa from Turkey. Food Chemistry, 141, 91-97. [CrossRef] google scholar
  • Albayrak, S., Atasagun, B., Aksoy, A. (2017). Comparison of phe-nolic components and biological activities of two Centaurea sp. obtained by three extraction techniques”, Asian Asian Pasidic Journal of Tropical Medicine, 10(6), 599-606. [CrossRef] google scholar
  • Ayaz, F.A., Özcan, M, Kurt, A., Karayiğit, B., Ozogul, Y., Glew, R. & Ozogul F. (2017). Fatty acid composition and antioxidant capacity of cypselas in Centaurea s.l. taxa (Asteraceae, Cardueae) from NE Anatolia. South African Journal of Botany, 112, 474- 482. [CrossRef] google scholar
  • Baytop, T. (1999). Therapy with medicinal plants in Turkey, Past and Present, second ed., Nobel Tıp Basımevi, Istanbul, Turkey. google scholar
  • Boğa, M., Alkan, H., Ertaş, A., Oral, E.V., Yılmaz, M.A., Yeşil, Y.... & Kolak, U. (2016). Phytochemical profile and some biological ac-tivities of three Centaurea species from Turkey. Tropical Journal of Pharmaceutical Research, 15 (9), 1865-1875. [CrossRef] google scholar
  • Çelik, S.E. (2011). Farklı tür, karışım ve çözücü ortamlarına uygulanabilen modifiye Cuprac antioksidan kapasite ölçümleri. İstanbul Üniversitesi, Fen bilimleri Enstitüsü. google scholar
  • Erdogan, T., Gonenc, T., Cakilcioglu, U. & Kivcak, B. (2014). Fatty acid composition of the aerial parts of some Centaurea species in Elazıg, Turkey. Tropical Journal of Pharmaecutical Research, 13(4), 613-616. [CrossRef] google scholar
  • Esmaeili, M.A. & Sonboli, A. (2010). Antioxidant, free radical scav-enging activities of Salvia branchyantha and its protective effect against oxidative cell injury. Food Chemistry and Toxicology, 48, 846-853. [CrossRef] google scholar
  • Janackovic, P., Gavrilovic, M., Vujisic, L., Matevski, V. & Marin, P.D. (2017). Fatty acid composition of the cypcelae of two endemic Centaurea species (Asteraceae). Botanical Serbica, 41 (1), 3-9. [CrossRef] google scholar
  • Köse, Y.B., İşçan, G., Göger, F., Akalın, G., Demirci, B. & Başer, K.H.C. (2016). Chemical composition and biological activity of Centaurea baseri: New species from Turkey. Chemical Biodiver-sity, 13, 1369 -1379. [CrossRef] google scholar
  • Lazari, D., Skaltsa H., Constantinidis, T. (2000). Volatile constituents of Centaurea pelia DC., C. thessala Hausskn. subsp. drakiensis (Freyn & Sint.) Georg. and C. zuccariniana DC. from Greece. Flavour and Fragrance Journal, 15(1), 7- 11. google scholar
  • National Research Council (US) Comittee on Diet and Health. (1989). Diet and Health: Implications for Reducing Chronic Disease Risk. National Academies Press. Fats and Other Lipids. Hard copy ver-sion at National Academies Press, Washington DC. google scholar
  • Oyaizu, M. (1986). Studies on product of browning reaction prepared from glucose amine. The Japanese Journal of Nutrition and Di-etetics, 44, 307. [CrossRef] google scholar
  • Ros, E. & Mataix, J. (2006) Fatty acid composition of nuts- implica-tions for cardiovascular health. British Journal of Nursing, 96 (2), 29-35. [CrossRef] google scholar
  • Senatore, F., Formisano, C., Raio, A., Bellone G. & Bruno M. (2008). Volatile components from flower-heads of Centaurea nicaeensis All., C. parlatoris Helder and C. solstitialis L. ssp. schouwii (DC.) Dostal growing wild in Southern Italy and their biological activity. Natural Product Research, 22(10), 825- 832. [CrossRef] google scholar
  • Tastan, P., Tuzun, B., Kivcak, B. & Fafal T. Fatty acids and antioxidant capacities of three Centaurea L. species. J Pharm Res. Article in Press. google scholar
  • Tekeli, Y., Zengin, G., Aktümsek, A., Sezgin, M. & Torlak, E. (2011). Antibacterial activities of extracts from twelve Centaurea species from Turkey. Archieves of Biological Sciences, 63(3), 685-690. [CrossRef] google scholar
  • UPAC. (1979). Standards methods for analysis of oils, fats and deriva-tives. 6th ed., Pergamon Press, Oxford, 59-66. google scholar
  • Yıldırım, N., Sunar, S., Agar, G., Bozarı, S.& Aksakal, O. (2009). Biochemical and molecular characterization of some Centaurea species growing in the Eastern Anatolia region of Turkey. Bio-chemical Genetics, 47, 850-859. [CrossRef] google scholar
  • Zengin, G., Aktümsek, A., Güler, G.Ö., Çakmak, Y.S.& Yıldıztugay, E. (2011). Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. subsp. hayekiana Wa-genitz. Records of Natural Products, 5 (2), 123-132. google scholar
  • Zengin, G., Çakmak, Y.S., Guler, G.O. & Aktumsek, A. (2010). In vitro antioxidant capacities and fatty acid compositions of three Cen-taurea species collected from Central Anatolia region of Turkey. Food Chemistry and Toxicology, 48, 2368-2341. [CrossRef] google scholar
  • Zengin, G., Guler, G.O., Cakmak, Y.S. & Aktumsek, A. (2011). An-tioxidant capacity and fatty acid profile of Centaurea kotschyi (Boiss.& Heldr.) Hayek var. persica (Boiss.) Wagenitz from Turkey. Grasas Y Acaites, 62 (1), 90- 95. [CrossRef] google scholar
  • Zengin, G., L-lorent-Martinez, E., Sinan, K.I., Yıldıztugay, E., Picot-Allain, C.&Mahomoodally, M.F. (2019a). Chemical profiling of Centaurea bornmuelleri Hausskn. aerial parts by HPLC-MS/MS and their pharmaceutical effects: From nature to novel perspec-tives. Journal of Pharmaceutical and Biomedical Analysis, 174, 406-413. [CrossRef] google scholar
  • Zengin, G., Zheleva-Dimitrovab, D., Gevrenovab, R., Aktumsek, A., Sinan, K.I. & Mahomood, M.F. (2019b). A comparative assess- google scholar
  • ment of the LC-MS profiles and cluster analysis of four Centaurea species from Turkey. Biocatalysis Agricultural Biotechnology, 20 (2019), 10189. [CrossRef] google scholar
  • Zengin, G., Zhelava-Dimitrova, D., Gevrenova, R., Nedialkov, P, Mo-can, A....& Ciric, A. (2018). Identification of phenolic compo- google scholar
  • nents via LC-MS analysis and biological activities of two Centau-rea species: Centaurea drabifolia subsp. drabifolia and Centaurea lycopifolia. Journal of Pharmaceutical and Biomedical Analysis, 149, 436- 441. [CrossRef] google scholar
There are 28 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Original Article
Authors

Burcu Sümer Tüzün 0000-0002-9194-3447

Tuğçe Fafal 0000-0002-7445-5855

Bijen Kıvçak 0000-0001-7645-1930

Publication Date August 26, 2024
Submission Date December 14, 2021
Published in Issue Year 2024 Volume: 54 Issue: 2

Cite

APA Sümer Tüzün, B., Fafal, T., & Kıvçak, B. (2024). Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam. İstanbul Journal of Pharmacy, 54(2), 182-188. https://doi.org/10.26650/IstanbulJPharm.2024.1036392
AMA Sümer Tüzün B, Fafal T, Kıvçak B. Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam. iujp. August 2024;54(2):182-188. doi:10.26650/IstanbulJPharm.2024.1036392
Chicago Sümer Tüzün, Burcu, Tuğçe Fafal, and Bijen Kıvçak. “Determining the Fatty Acid Composition and Antioxidant Activities of Centaurea Virgata Lam”. İstanbul Journal of Pharmacy 54, no. 2 (August 2024): 182-88. https://doi.org/10.26650/IstanbulJPharm.2024.1036392.
EndNote Sümer Tüzün B, Fafal T, Kıvçak B (August 1, 2024) Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam. İstanbul Journal of Pharmacy 54 2 182–188.
IEEE B. Sümer Tüzün, T. Fafal, and B. Kıvçak, “Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam”., iujp, vol. 54, no. 2, pp. 182–188, 2024, doi: 10.26650/IstanbulJPharm.2024.1036392.
ISNAD Sümer Tüzün, Burcu et al. “Determining the Fatty Acid Composition and Antioxidant Activities of Centaurea Virgata Lam”. İstanbul Journal of Pharmacy 54/2 (August 2024), 182-188. https://doi.org/10.26650/IstanbulJPharm.2024.1036392.
JAMA Sümer Tüzün B, Fafal T, Kıvçak B. Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam. iujp. 2024;54:182–188.
MLA Sümer Tüzün, Burcu et al. “Determining the Fatty Acid Composition and Antioxidant Activities of Centaurea Virgata Lam”. İstanbul Journal of Pharmacy, vol. 54, no. 2, 2024, pp. 182-8, doi:10.26650/IstanbulJPharm.2024.1036392.
Vancouver Sümer Tüzün B, Fafal T, Kıvçak B. Determining the fatty acid composition and antioxidant activities of Centaurea virgata Lam. iujp. 2024;54(2):182-8.