First screening of volatile constituents and antibacterial, antibiofilm, and anti-quorum sensing activities of Cinclidotus species

Year 2024, Volume: 54 Issue: 3, 424 - 434, 30.12.2024

Mesut Kırmacı , Alev Önder , Ahsen Sevde Çınar Koç , Suna Sibel Rızvanoğlu , Müjde Eryılmaz , Özlem Bahadır Acıkara , Gözde Aslan , Angel Antonio Carbonell Barrachina

https://doi.org/10.26650/IstanbulJPharm.2024.1418624

Abstract

Background and Aims: Bryophytes are the center of interest for natural sources due to their medicinal and traditional usage in various ailments with their interesting phytochemicals. However, there are very few studies on this subject. In the present study, we aimed to investigate mosses belonging to the genus Cinclidotus (Cinclidotaceae), such as C. pachyloma, C. bistratosus, C. riparius, C. pachylomoides, C. fontinaloides and C. aquaticus regarding their volatile components and biological activities.
Methods: The mosses were collected from various locations in Türkiye and then extracted with ether. The volatile components were identified and semi-quantified using GC-TQMS (Gas Chromatography and a Triple Quadrupole Mass Spectrometer). The MICs were evaluated using the broth microdilution method. A microplate-based biofilm model was used against P. aeruginosa PAO1 using the crystal violet assay to determine the antibiofilm activity. The anti-quorum sensing activity was carried out using the disc diffusion method.
Results: The initial screening of the selected mosses to confirm the significant potential of their volatile phytochemicals was investigated for the first time in this study. The main components were determined as linoelaidic acid, glycerol 2-hexadecanoate, and campesterol in diverse types of Cinclidotus species by GC/TQMS. In addition, the potency of the antibacterial, antibiofilm, and anti-quorum sensing activities of these species exhibited moderate to highly effective results.
Conclusion: All results showed that mosses are rich sources of natural compounds and good samples for biological activities. Mosses are promising candidates that could be useful in preventing or treating various pathological conditions. However, further in vitro and in vivo studies should focus on a single component or the mechanisms.

Keywords

Bryophyte, Metabolites, Moss, Phytochemicals, Activity, Volatile

Ethical Statement

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