Prognostic significance of NF-κB in resectable gastric cancer treated with concomittant chemoradiotherapy

Year 2007, Volume: 22 Issue: 2, 69 - 73, 01.03.2007

Oğuz Galip Yıldız Serdar Soyuer İşın Soyuer Kadir Uçar Mete Gündoğ Bünyamin Kaplan Metin Özkan

Abstract

OBJECTIVES The aim of this study is to determine the prognostic value of transcription factor nucleer factor kappa B ( N F -κB) in resectable gastric cancer patients treated with concurrent chemoradiotherapy. METHODS Forty-nine (35 males, 14 females; median age 55; range 2273 years) resectable gastric cancer patients treated with gastrectomy and adjuvant concurrent chemoradiotherapy were enrolled in this study. All patients received weekly 250 mg/ m2 5-FU chemotherapy and radiotherapy concomittantly after gastrectomy. Then patients received 4 cycles i.v bolus FUFA chemotherapy sequentialy as consolidation treatment 2-4 week after chemoradiotherapy. Immunohistochemistry staining was performed on paraffin embedded tissue. NF-κB monoclonal antibodies were used for the staining. RESULTS The progression free surv ival and overall surv ival dif er e nce were anal ised between NF-κB pos it ive and neg at ive groups. For NF-kB a specimen was judged posit ive if more than 10% of the total cancer cells in a single field of view showed a response. CONCLUSION In patients who NF-κB was stained more than 10%, the overall survival was worse and there was statistically significant difference between groups. But in two groups, no statistically significant difference due to disease free survival.

Keywords

Gastrectomy, Chemotherapy, Adjuvant, Stomach Neoplasms, NF-kappa B, Survival Rate, Radiotherapy, Radiation Oncology

Gastrektomi sonrası adjuvan kemoradyoterapi uygulanan mide karsinomlu olgularda NF-kB'nin prognostik önemi

Abstract

AMAÇ Gastrektomiden sonra adjuvan kemoradyoterapi uygulanan mide kanserli olgularda transkripsiyon faktör nükleer faktör kappa B'nin (NF-κB ) progresyonsuz sağkalım ve genel sağkalım yönünden prognostik önemi araştırıldı. GEREÇ VE YÖNTEM Çalışmaya evre II-IV mide karsinomlu gastrektomi ve adjuvan kemoradyoterapi ile tedavi edilen 49 olgu (35 erkek, 14 kadın; median yaş 55; dağılım 22-73) dahil edildi. Tüm olgulara gastrektomi sonrası adjuvan radyoterapi ile eş zamanlı haftada bir kez 250 mg/m2 dozunda haftalık i.v. bolus 5-FU verildi. Kemoradyoterapinin tamamlanmasından 2-4 hafta sonra konsolidasyon amaçlı ardışık dört kür Mayo Rejimi i.v. bolus tarzında uygulandı. Olgulara ait parafin bloklardan yeni kesitler alınarak NF-kB immünohistokimyasal boyası uygulandı. NF-kB için tümör dokusunda nükleer boyanmanın %10'dan az olan olgular negatif, %10'dan fazla olan olgular ise pozitif olarak değerlendirildi. BULGULAR Bu kritere göre yapılan değerlendirmede toplam 49 hastanın 29'unda (%59) NF-kB negatif, 20 hastada (%41) ise NF-kB pozitif boyandı. Gruplar arasında progresyonsuz sağkalım ve genel sağkalım açısından fark olup olmadığı araştırıldı. SONUÇ NF-kB pozitif (%10'dan fazla boyanan) grupta genel sağkalım istatistiksel olarak anlamlı ve daha kısa bulunmuşken, hastalıksız sağkalım yönünden iki grup arasında istatistiksel anlamlı fark gösterilemedi.

Keywords

Kemoradyoterapi, mide kanseri/patoloji, immünohistokimya, NF-kappa B, prognostik faktör, sağkalım oranı

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