Protective effect of N-acetyl cysteine on radiation-induced DNA damage in rat bone marrow: a comparison with WR-2721

Abstract

OBJECTIVES To evaluate the potential radioprotective effects of N-acetylcysteine (NAC) against genocytotoxicity. As representative of a clinically used radioprotector, the effect of WR-2721 was compared with that of NAC using chromosomal aberration (CA) and mitotic index (MI) in the irradiated rat's femoral bone marrow cells. METHODS The rats (n=48) were divided randomly and equally into six groups as: Control (C), NAC (received 1000 mg/kg NAC), WR-2721 (200 mg/kg WR-2721), Radiation (R, received irradiation), R + NAC (received irradiation and 1000 mg/kg NAC), and R + WR-2721 (received irradiation and 200 mg/kg WR-2721). All the irradiated groups received whole-body gamma irradiation as a single dose of 6 Gy. At 72th hours, the rats were sacrificed and bone marrow cells were bilaterally collected from rats' femur. Then, cytogenetic and cytotoxicity tests were performed according to convantional methods. RESULTS Group R showed significantly higher CA and lower MI values when compared to C. R + NAC and R + WR-2721 groups showed significantly lower CA and higher MI averages when compared to R. CONCLUSION The results give clues about the beneficial effects of NAC against radiation-induced genocytotoxicity.

Keywords

• Acetylcysteine,Radiation Injuries,Radiation Injuries, Experimental,Cytogenetic Analysis,Rats, Wistar,Chromosome Aberrations,Mitotic Index,Female,Amifostine,Bone Marrow,Radiation-Protective Agents

N-asetil-sistein'in sıçan kemik iliği hücrelerinde radyasyona bağlı DNA hasarından koruyucu etkisi ve WR-2721 ile karşılaştırması

Öz

AMAÇ Deneysel çalışmamızda radyasyonun biyolojik sistemlerde neden olduğu genotoksik etkilere karşı, N-asetil-sisteinin (NAS) koruyucu etkileri konvensiyonel sitogenetik yöntemlerle (kromozom aberasyonu-CA, mitotik indeks-MI) amifostinle (WR-2721) karşılaştırıldı. GEREÇ VE YÖNTEM Co-60 cihazı ile 6 Gy tek doz tüm vücudu ışınlanan sıçanların femur-kemik ilikleri incelendi. Kontrol (K); radyasyon (R); NAS; amifostin (WR-2721) R + NAS (R + NAS); R + WR-2721 gruplarında, eşit sayıda toplam 48 dişi sıçan kullanıldı. R grubu yalnız radyasyon, R + WR-2721 grubuna radyasyon öncesi WR-2721 (200 mg/kg, i.p.), R + NAS grubuna NAS (1000 mg/kg, i.p.) verildi; 72 saat sonra tüm grupların preperasyonu konvensiyonel sitogenetik yöntemlerle hazırlanarak incelendi. BULGULAR R grubunun ortalama CA değeri en yüksek, MI değeri düşük olup; kontrol grubuna göre bu fark anlamlıydı. R + NAS ve R + WR-2721 gruplarında R grubuna göre hasar göstergeleri daha düşüktü; farklar istatistiksel olarak anlamlıydı. SONUÇ Bu sonuçlara göre, iyonize radyasyonun erken dönemdeki genotoksik etkilerine karşı amifostin ve NAS'nin koruyucu etkilerinin benzer olduğunu söylenebilir

Anahtar Kelimeler

• N-asetilsistein, radyasyon hasarı, WR-2721

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