Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat

Mehrdad Pourjafar; Khalil Badıeı; Hassan Sharıfıyazdı; Seyed Naghıb; Aliasghar Chalmeh; Mohammad Dıvar

doi:10.16988/iuvfd.17233

Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat

Abstract

ABSTRACT

The present study describes a rare case of freemartinism of the 60XX/60XY chimera type in an adult 7-year-old horned, mixed Lori breed phenotypically doe. Signs of masculinisation were evident in both the behavioral patterns and body conformation, but there was no structure similar to penis and hypospadias was observed in this goat. External genital organs were similar to that of a female one. In this study, a single multiplex PCR assay was used to determine the freemartinism status (XX-XY blood chimerism) based on semi-quantitatively co amplify a sex-based polymorphism in the amelogenin locus (AMX and AMY). In addition, serum concentrations of anti-Müllerian hormone (AMH), testosterone and progesterone hormones of this horned intersex goat were evaluated. Serum AMH levels in the present case (0.2 ng/ml) were similar to male (0.2 ng/ml) and lower than female (0.6 ng/ml) goat. The serum concentrations of testosterone (0.2 ng/ml) were remarkably high in comparison to normal control male and female ones (0.02 ng/ml). Progesterone level in the intersex goat (0.4 ng/ml) was close to control buck (0.7 ng/ml) and remarkably lower than control doe (10 ng/ml). The results of the present study showed that the use of hormonal pattern, especially AMH level, and single multiplex PCR technique provide an easy and alternative approaches for indication of XX/XY chimerism or mosaicism in intersex freemartin goats.

Key Words: Freemartin, XX/XY chimerism, intersex, horned goat, single multiplex PCR, anti-müllerian hormone

ÖZET

BOYNUZLU BİR KEÇİDE FREEMARTİNİZMİN HORMONAL VE TEKLİ MULTİPLEKS PCR ANALİZ

UYGULAMALARI İLE TESPİTİ

Bu çalışmada, nadir bir olgu olan freemartinizmin dişi fenotipine sahip Lori ırkı melezi 7 yaşındaki boynuzlu ergin bir keçide 60XX/XY kimera tipi bildirilmektedir. Davranış biçimlerinde ve vücut yapısında maskülinizasyon belirtileri bulunur iken, penis veya hypospadias gözlenmemiştir. Dış genital organlar dişi keçi ile benzerlik göstermektedir. Bu çalışmada amelogonin lokusundaki (AMX ve AMY) cinsiyete bağlı polimorfizmin semi-kantitatif olarak çoğaltılması ile freemartinizm durumunun (XX-XY kan kimerizmi) belirlenmesinde tekli multipleks PCR yöntemi kullanılmıştır. Ayrıca anti-Müllerian hormon (AMH), testesteron ve progesteron hormonlarının serum konsantrasyonları da değerlendirmeye alınmıştır. Mevcut vakadaki serum AMH düzeyleri (0,2 ng/ml) tekeye benzer (0,2 ng/ml) olarak, dişi keçiden (0,6 ng/ml) ise düşük olarak saptanmıştır. Serum testesteron konsantrasyonu (0,2 ng/ml) kontrol tekeye ve dişi keçiye (0,02 ng/ml) göre önemli düzeyde yüksek bulunmuştur. İnterseks keçide progesteron düzeyi (0,4 ng/ml) kontrol tekedeki düzeye (0,7 ng/ml) yakın, kontrol dişi keçideki düzeyden (10 ng/ml) ise belirgin olarak düşüktür. Mevcut çalışmanın sonuçları interseks freemartin keçilerde XX/XY kimerizmi veya mozaizmini belirlemede AMY düzeyi ve tekli multipleks PCR tekniğinin kolay ve alternatif bir yöntem olduğunu ortaya koymaktadır.

Anahtar Kelimeler: Freemartin, XX/XY kimerizmi, interseks, boynuzlu keçi, tekli mutlipleks PCR, anti-müllerian hormon

Keywords

Freemartin, XX/XY kimerizmi, interseks, boynuzlu keçi, tekli mutlipleks PCR, antimüllerian hormon

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Mehrdad Pourjafar This is me

Khalil Badıeı This is me

Hassan Sharıfıyazdı This is me

Seyed Naghıb This is me

Aliasghar Chalmeh This is me

Mohammad Dıvar This is me

Publication Date

November 15, 2012

Submission Date

November 15, 2012

Acceptance Date

-

Published in Issue

Year 2012 Volume: 38 Number: 2

DOI

https://doi.org/10.16988/iuvfd.17233

IZ

https://izlik.org/JA64LA97ME

Cite

RIS / Bibtex

APA

Pourjafar, M., Badıeı, K., Sharıfıyazdı, H., Naghıb, S., Chalmeh, A., & Dıvar, M. (2012). Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 38(2), 175-181. https://doi.org/10.16988/iuvfd.17233

AMA

1.Pourjafar M, Badıeı K, Sharıfıyazdı H, Naghıb S, Chalmeh A, Dıvar M. Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat. iuvfd. 2012;38(2):175-181. doi:10.16988/iuvfd.17233

Chicago

Pourjafar, Mehrdad, Khalil Badıeı, Hassan Sharıfıyazdı, Seyed Naghıb, Aliasghar Chalmeh, and Mohammad Dıvar. 2012. “Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 38 (2): 175-81. https://doi.org/10.16988/iuvfd.17233.

EndNote

Pourjafar M, Badıeı K, Sharıfıyazdı H, Naghıb S, Chalmeh A, Dıvar M (November 1, 2012) Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 38 2 175–181.

IEEE

[1]M. Pourjafar, K. Badıeı, H. Sharıfıyazdı, S. Naghıb, A. Chalmeh, and M. Dıvar, “Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat”, iuvfd, vol. 38, no. 2, pp. 175–181, Nov. 2012, doi: 10.16988/iuvfd.17233.

ISNAD

Pourjafar, Mehrdad - Badıeı, Khalil - Sharıfıyazdı, Hassan - Naghıb, Seyed - Chalmeh, Aliasghar - Dıvar, Mohammad. “Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 38/2 (November 1, 2012): 175-181. https://doi.org/10.16988/iuvfd.17233.

JAMA

1.Pourjafar M, Badıeı K, Sharıfıyazdı H, Naghıb S, Chalmeh A, Dıvar M. Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat. iuvfd. 2012;38:175–181.

MLA

Pourjafar, Mehrdad, et al. “Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 38, no. 2, Nov. 2012, pp. 175-81, doi:10.16988/iuvfd.17233.

Vancouver

1.Mehrdad Pourjafar, Khalil Badıeı, Hassan Sharıfıyazdı, Seyed Naghıb, Aliasghar Chalmeh, Mohammad Dıvar. Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat. iuvfd. 2012 Nov. 1;38(2):175-81. doi:10.16988/iuvfd.17233