OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER

Ş. Atalgın; İbrahim Kurtul; Sami Özcan; Emine Bozkurt

doi:10.16988/iuvfd.67312

OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER

Abstract

ABSTRACT

Recent researches in experimental embryology of avian species have particularly investigated skeletal mutations, development of cultured embryos under artificial conditions, and teratogenic consequences of new drugs. Data on the normal skeletogenous stages are also essential parameters to interpret and elucidate the data acquired on these studies. Accordingly, a number of 10 embryos were prepared every 24 h from 10 to 20 days of incubation, to report the ossification and growth of the ribs and sternum of the Hubbert strain broiler (JV). The embryos fixed in 10% formaldehyde then 95% ethanol solutions were cleared and the skeletons of the embryos were stained in toto with Alcian blue&alizarin red for cartilage and ossified components, respectively. Observation of the skeletons photography were performed under a stereoscopic microscopy, paying particular attention to the timing of chondrification and ossification of the bones. The first occurrence of the ossification was observed in the ribs on the 11th day of incubation. Those in the sternum were seen on the 15^th day. We observed in this study that the uncinate processes of the ribs showed very early chondrification and ossification. The ossified components continued to develop until the 20^th day of incubation when the hatching took place.

Key Words: Broiler, ossification, rib, sternum

KULUÇKA DÖNEMİNDE HUBBERT IRKI BROYLERLERDE STERNUM VE KOSTALARIN KEMİKLEŞME VE GELİŞİMİNİN BELİRLENMESİ

ÖZET

Son yıllarda kanatlılar üzerinde yapılan deneysel embriyolojik çalışmalar özellikle iskelet mutasyonları, yapay ortamlarda embriyo kültürlerinin gelişimi ve yeni ilaçların teratolojik etkilerini içermektedir. Normal iskelet gelişimi verileri, bu çalişmaların yorumlanması ve doğrulanması için vazgeçilmez parametrelerdir. Araştırmada, inkubasyonun 10. gününden 20. güne kadar her gün 10 adet Hubbert (JV) ırkı embriyosu kuluçka makinesinden çıkartılarak sternum ve kostaların kemikleşmesi ve gelişimi gözlemlendi. %10'luk formaldehit solüsyonunda ve %95'lik ethanolde tespit edilen embriyolar temizlenerek, kıkırdak ve kemik dokuların ayrı renklerde tespiti için alcian blue ve alizarin red ile boyandı. İskelet sisteminin gözlenmesi ve fotoğraf çekimleri, özellikle kıkırdaklaşma ve kemikleşme zamanları dikkate alınarak, stereoskopik mikroskopla yapıldı. Kostalarda ilk kemikleşme merkezi inkubasyonun 11. gününde saptandı. Sternumun kemikleşmesinin ise 15. günde başladığı gözlendi. Çalışmada, kostalarda bulunan Proccessus uncinatus'ların çok erken kıkırdaklaşma ve kemikleşme sürecine başladığı tespit edildi. Kemikleşmiş kısımların gelişiminin inkubasyonun 20. gününe kadar devam ettiği saptandı.

Anahtar Kelimeler: Broyler, Kosta, Kemikleşme, Sternum

Keywords

Broyler, Kosta, Kemikleşme, Sternum

References

Blom, J . and Lilja, C.: A comparative study of embryonic development of some bird species with different patterns of postnatal growth. Zool. 2005; 108: 81-95.
Blom, J . and Lilja, C.: A comparative study of growth, skeletal development and eggshell composition in some species of birds. J. Zool. 2004. (London). 262: 361-369.
Etches, R.J., Carsience, R.S., Clark, M.E., Fraser, R.A., Toner, A., Verrinder Gibbins,A. M.:Chimeric chickens and their use in manipulation of the chicken genome. Poult. Sci, 1993; 72: 882-889.
Hamburger, H. and Hamilton H.L.: A serious of normal stages in the development of the chick embryo. J. Morphol, 1951; 88: 49-92.
Hamilton, H.L.: Lillie's development of the chick: An introduction to embryology. Holt, Rinchart, Winston, New York. 1952.
Hashizume, R., Noda, A., Itoh, M., Yamamoto, Y., Masui, S., Oka, M.: A method for detecting malformations in chicken embryos. Jap. Poult. Sci,. 1993; 30: 298-305.
Hashizume, R., Noda, A., Itoh, M.: Studies on teratological testing using chicken embryos: effects of solvents, injection sites and the age of the embryo. Exp. Anim, 1992; 41: 349-356.
Jollie, M. T.: The head skeleton of the chicken and remarks on the anatomy of this region in other birds. J. Morphol, 1957; 100: 389-436.

Lilja, C., Blom, J. , Marks, H.L.: A comparative study of embryonic development of Japanese quail selected for different patterns of postnatal growth. Zool, 2001; 104: 115-122.
Naito, M., Nirasawa, K., Oishi, T.: Development in culture of the chick embryo from fertilized ovum to hatching. J. Exp. Zool, 1990; 254: 322-326.
Nakane, Y. and Tsudzuki, M.: Development of the skeleton in Japanese quail embryos. Dev. Grow. Differ., 1999; 41: 523-534.
Nakane, Y. and Tsudzuki, M.: Morphological and genetic studies for a new morphometric mutant of Japanese quail. Proceedings, 6th Asian Pacific Poultry Congress, 1998; 242-243.
Julian J.B.: Nomina Anatomica Avium, Handbook of Avian Anatomy Cambridge, Massachusetts. Published by The Nuttall Ornithological Club. No: 23, 1993.
Ono, T., Matsumoto, T., Arisawa, Y.: Production of donor-derived offspring by transfer of primordial germ cells in Japanese quail. Exp. Anim., 1998; 47: 215-219.
Ono, T., Murakami, T., Mochii, M.: A complete culture system for avian transgenesis, supporting quail embryos from the single-cell stage to hatching. Dev. Biol, 1994; 161: 126-130.
Padgett, C.S. and Ivey, W.D.: The normal embryology of the coturnix quail. Anat. Rec, 1960; 137: 1-11.
Perry, M.M.: A complete culture system for the chick embryo. Nature, 1988; 331, 70-72.
Peters, P.W.J., Neubert, D., Merker, H. J. , Kwasogroch, T. E.: Double staining of foetal skeletons for cartilage and bone. Methods in prenatal toxicology. George Thieme, Stuttgart, 1977.
Ricklefs, R.E. , Starck, J . M., Konarzewski, M.: Internal constraints on growth in birds. Avian growth and development. Evolution within the altricial-precocial spectrum. pp. 266-287. In: Stack, J. M. , Ricklefs, R. E. (eds.), Oxford University Press., Oxford. 1998.
Tsudzuki, M., Nakane, Y., Wada, A.: Hereditary multiple malformation in Japanese quail: A possible powerful animal model for morphogenetic studies. J. Hered., 1998; 89: 24-31.
Zacchei, A.M.: Lo sviluppo embrionale della guaglia giapponese. Arch. It. Anat. Embriol. 1961; 66: 36-62.

Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Ş. Atalgın This is me

İbrahim Kurtul This is me

Sami Özcan This is me

Emine Bozkurt This is me

Publication Date

November 20, 2012

Submission Date

November 20, 2012

Acceptance Date

-

Published in Issue

Year 2008 Volume: 34 Number: 1

DOI

https://doi.org/10.16988/iuvfd.67312

IZ

https://izlik.org/JA22WB97ME

Cite

RIS / Bibtex

APA

Atalgın, Ş., Kurtul, İ., Özcan, S., & Bozkurt, E. (2012). OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 34(1), 1-8. https://doi.org/10.16988/iuvfd.67312

AMA

1.Atalgın Ş, Kurtul İ, Özcan S, Bozkurt E. OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER. iuvfd. 2012;34(1):1-8. doi:10.16988/iuvfd.67312

Chicago

Atalgın, Ş., İbrahim Kurtul, Sami Özcan, and Emine Bozkurt. 2012. “OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34 (1): 1-8. https://doi.org/10.16988/iuvfd.67312.

EndNote

Atalgın Ş, Kurtul İ, Özcan S, Bozkurt E (November 1, 2012) OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34 1 1–8.

IEEE

[1]Ş. Atalgın, İ. Kurtul, S. Özcan, and E. Bozkurt, “OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER”, iuvfd, vol. 34, no. 1, pp. 1–8, Nov. 2012, doi: 10.16988/iuvfd.67312.

ISNAD

Atalgın, Ş. - Kurtul, İbrahim - Özcan, Sami - Bozkurt, Emine. “OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34/1 (November 1, 2012): 1-8. https://doi.org/10.16988/iuvfd.67312.

JAMA

1.Atalgın Ş, Kurtul İ, Özcan S, Bozkurt E. OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER. iuvfd. 2012;34:1–8.

MLA

Atalgın, Ş., et al. “OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 34, no. 1, Nov. 2012, pp. 1-8, doi:10.16988/iuvfd.67312.

Vancouver

1.Ş. Atalgın, İbrahim Kurtul, Sami Özcan, Emine Bozkurt. OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER. iuvfd. 2012 Nov. 1;34(1):1-8. doi:10.16988/iuvfd.67312