DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY

Şükrü Kırkan; Ergün Göksoy; Serten Tekbıyık; Osman Kaya

doi:10.16988/iuvfd.21905

DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY

Abstract

ABSTRACT

The presence of Yersinia ruckeri in rainbow trout hatchery farms was investigated by PCR Assay. Twenty-five rainbow trout, suspected of Enteric Redmouth Disease, were examined in this study. Yersinia ruckeri strains were identified from 8 out of 25 samples (32%). Ten of 25 (40%) rainbow trout samples showed positive reactions for Yersinia ruckeri in the PCR. All PCR positive samples have shown specific amplification bands of the 575-bp fragment for Yersinia ruckeri.

Key Words: Yersinia ruckeri, Rainbow trout, PCR, diagnosis.

TÜRKİYE'NİN BATISINDAKİ GÖKKUŞAĞI ALABALIK YETİŞTİRME ÇİFTLİKLERİNDE YERSİNİA RUCKERİ'NİN PZR KULLANILARAK TANIMLANMASI

ÖZET

Bu araştırmada, Gökkuşağı Alabalığı çiftliklerindeki Yersinia ruckeri varlığı Polimeraz Zincir Reaksiyonu (PZR) metodu ile incelendi. Bu çalışmada Kızıl Ağız Hastalığı §şüpheli 25 adet Gökkuşağı Alabalığı materyal olarak kullanıldı. 25 örneğin 8 (%32)'inden Yersinia ruckeri identifiye edildi. 25 örneğin 10'unda PZR'da Yersinia ruckeri yönünden pozitiflik saptandı. PZR pozitif ürünlerin hepsinde, Yersinia ruckeri için spesifik 575 bp uzunluğunda bantlar elde edildi.

Anahtar Kelimeler: Yersinia ruckeri, Gökkuşağı Alabalığı, PZR, tanı.

Keywords

Yersinia ruckeri, Gökkuşağı Alabalığı, PZR, tanı

References

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Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Şükrü Kırkan

Ergün Göksoy This is me

Serten Tekbıyık This is me

Osman Kaya This is me

Publication Date

December 21, 2012

Submission Date

December 21, 2012

Acceptance Date

-

Published in Issue

Year 2006 Volume: 32 Number: 3

DOI

https://doi.org/10.16988/iuvfd.21905

IZ

https://izlik.org/JA65CT34JW

Cite

RIS / Bibtex

APA

Kırkan, Ş., Göksoy, E., Tekbıyık, S., & Kaya, O. (2012). DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 32(3), 21-30. https://doi.org/10.16988/iuvfd.21905

AMA

1.Kırkan Ş, Göksoy E, Tekbıyık S, Kaya O. DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY. iuvfd. 2012;32(3):21-30. doi:10.16988/iuvfd.21905

Chicago

Kırkan, Şükrü, Ergün Göksoy, Serten Tekbıyık, and Osman Kaya. 2012. “DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 32 (3): 21-30. https://doi.org/10.16988/iuvfd.21905.

EndNote

Kırkan Ş, Göksoy E, Tekbıyık S, Kaya O (December 1, 2012) DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 32 3 21–30.

IEEE

[1]Ş. Kırkan, E. Göksoy, S. Tekbıyık, and O. Kaya, “DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY”, iuvfd, vol. 32, no. 3, pp. 21–30, Dec. 2012, doi: 10.16988/iuvfd.21905.

ISNAD

Kırkan, Şükrü - Göksoy, Ergün - Tekbıyık, Serten - Kaya, Osman. “DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 32/3 (December 1, 2012): 21-30. https://doi.org/10.16988/iuvfd.21905.

JAMA

1.Kırkan Ş, Göksoy E, Tekbıyık S, Kaya O. DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY. iuvfd. 2012;32:21–30.

MLA

Kırkan, Şükrü, et al. “DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 32, no. 3, Dec. 2012, pp. 21-30, doi:10.16988/iuvfd.21905.

Vancouver

1.Şükrü Kırkan, Ergün Göksoy, Serten Tekbıyık, Osman Kaya. DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY. iuvfd. 2012 Dec. 1;32(3):21-30. doi:10.16988/iuvfd.21905