Seroprevalence of Brucellosis in Livestock in Khuzestan Province, Southwest of Iran, 2008-2012

Abstract

ABSTRACT

Brucellosis is a zoonotic problem worldwide, especially in developing countries and specifically in Iran, where it is endemic. This study aimed to investigate the seroprevalence of brucellosis among livestock in the 18 districts of Khuzestan Province in Southwest Iran. Serum samples of 87798 cattle and 119020 sheep were tested using the Rose Bengal Plate Test, and positive results were confirmed with serum agglutination tests (SAT) and 2-mercaptoethanol SAT (2ME-SAT). The seroprevalence derived from total samples was 0.72% for cattle and 3.01% for sheep, revealing that though brucellosis is present among livestock populations in Khuzestan and that infection among sheep is significantly higher than among cattle (P<0.05), seroprevalence in general was much lower than indicated by results of other studies of livestock in the Middle East and neighboring regions. These results nevertheless recommend implementing a policy of transparency regarding brucellosis as well as measures to effectively eradicate the disease.

Key Words: Serology, ruminants, brucellosis, Iran

 

ÖZET 

2008-2012 YILLARI ARASINDA GÜNEYBATI İRAN KHUZESTAN İLİNDE YETİŞTİRİLEN
ÇİFTLİK HAYVANLARINDA BRUSELLOZUN SEROPREVALANSI

Bruselloz endemik seyrettiği İran ve gelişen ülkeler başta olmak üzere, dünya çapında yaygın görülen bir zoonotik hastalıktır. Çalışmada Güneybatı İran’da yer alan Khuzestan ilinin 18 farklı semtinde ki çiftlik hayvanları arasında brusellozun seroprevalansının araştırılması amaçlandı. 87798 sığır ve 119020 koyun serum örneği Rose Bengal Lam Aglütinasyon tekniği ile test edildi, pozitif örnekler serum aglütinasyon testi (SAT) ve 2-merkaptoetanol SAT (2ME-SAT) ile doğrulandı. Örneklerden elde edilen seroprevalans oranları, sığırlar için %0,72, koyunlarda ise %3,01 olup Khuzestan’da ki çiftlik hayvanlarında brusellozun varlığını ve sığırlara göre koyunlarda enfeksiyonun istatistiksel olarak daha yüksek oranda (P<0,05) seyrettiğini ortaya çıkardı. Bununla birlikte belirlenen genel seroprevalansın komşu bölgelerdeki ve Orta Doğu’daki çiftlik hayvanları üzerinde yapılan diğer çalışmalardaki sonuçlara göre çok daha düşük olduğu saptandı. Bu sonuçlar yine de bruselloz ile ilgili olarak şeffaflık politikası ile birlikte etkili eradikasyon tedbirlerinin uygulanması gerektiğini göstermektedir.

Anahtar Kelimeler: Seroloji, ruminant, bruselloz, İran

Keywords

• Serology, ruminants, brucellosis, Iran

2008-2012 YILLARI ARASINDA GÜNEYBATI İRAN KHUZESTAN İLİNDE YETİŞTİRİLEN ÇİFTLİK HAYVANLARINDA BRUSELLOZUN SEROPREVALANSI

Abstract

Bruselloz endemik seyrettiği İran ve gelişen ülkeler başta olmak üzere, dünya çapında yaygın görülen bir zoonotik hastalıktır. Çalışmada Güneybatı İran’da yer alan Khuzestan ilinin 18 farklı semtinde ki çiftlik hayvanları arasında brusellozun seroprevalansının araştırılması amaçlandı. 87798 sığır ve 119020 koyun serum örneği Rose Bengal Lam Aglütinasyon tekniği ile test edildi, pozitif örnekler serum aglütinasyon testi (SAT) ve 2-merkaptoetanol SAT (2MESAT) ile doğrulandı. Örneklerden elde edilen seroprevalans oranları, sığırlar için %0,72, koyunlarda ise %3,01 olup Khuzestan’da ki çiftlik hayvanlarında brusellozun varlığını ve sığırlara göre koyunlarda enfeksiyonun istatistiksel olarak daha yüksek oranda (P < 0,05) seyrettiğini ortaya çıkardı. Bununla birlikte belirlenen genel seroprevalansın komşu bölgelerdeki ve Orta Doğu’daki çiftlik hayvanları üzerinde yapılan diğer çalışmalardaki sonuçlara göre çok daha düşük olduğu saptandı. Bu sonuçlar yine de bruselloz ile ilgili olarak şeffaflık politikası ile birlikte etkili eradikasyon tedbirlerinin uygulanması gerektiğini göstermektedir.

Keywords

• Seroloji, ruminant, bruselloz, İran

References

1. Ahmed, M.O., Elmeshri, S.E., Abuzweda, A.R., Blauo, M., Abouzeed, Y.M., Ibrahim, A., Salem, H., Alzwam, F., Abid, S., Elfahem, A., Elrais, A., 2010. Seroprevalence of brucellosis in animals and human populations in the western mountains region in Libya, December 2006–January 2008. Eurosurveillance 15 (30), 1Ahmed, R., Munir, M.A., 1995. Epidemiological investigations of brucellosis in Pakistan. Pakistan Veterinary Journal 15, 169-172.
2. Ajmal, M., Ahmed, M.D., Arshad, M. 1989. Serosurveillance of brucellosis. Pakistan Veterinary Journal 9, 115-117.
3. Al-Majali, A.M., Talafha, A.Q., Ababneh, M.M., Ababneh, M.M., 2009. Seroprevalence and risk factors for bovine brucellosis in Jordan. Journal of Veterinary Science 10 (1), 61-65.
4. Alton, G.G., Jones, L.M., Pietz, D.E., 1975. Laboratory techniques in brucellosis. 2 nd Edition Geneva Press, WHO.
5. Amin, K.M.R., Rahman, M.B., Rahman, M.S., Han, J.C., Park, J.H., Chae, J.S., 2005. Prevalence of Brucella antibodies in sera of cows in Bangladesh. Journal of Veterinary Science 6, 223-2
6. Araj, G.F., Kattar, M.M., Fattouh, L.G., Bajakian, K.O., Kobeissi, S.A., 2005. Evaluation of the Panbio Brucella immunoglobulinG (IgG) and IgM enzymelinked immunosorbent assays for diagnosis of human brucellosis. Clinical and Diagnostic Laboratory Immunology 12 (11), 1334-1335.
7. Bale, J.O., Nuru, S., Addo, P.B., 1982. Serological study of sheep and goat brucellosis in Northern Nigeria. Bulletin of Animal Health and Production in Africa 30, 73-79.
8. Banai, M., 2002. Control of small ruminant brucellosis by use of Brucella melitensis rev.1 vaccine: Laboratory aspects and field observations. Veterinary Microbiology 90 (1-4), 497-519.

9. Baum, M., Zamir, O., Bergman-Rios, R., Katz, E., Beider, Z., Cohen, A., Banai, M., 1995. Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis. Journal of Clinical Microbiology 33 (8), 2166-2170.
10. Bercovich, Z., 1998. Maintenance of Brucella abortus free herds: A review with emphasis on the epidemiology and the problems in diagnosing brucellosis in areas of low prevalence. Veterinary Quarterly 20, 81-88.
11. Berto, W.J., Ajogi, I., Bale, J.O.O., Kwaga, J.K.P., Ocholi, R.A., 2010. Seroepidemiology of brucellosis in small ruminants in Plateau State, Nigeria. African Journal of Microbiology Research 4 (19), 1935-1938.
12. Blasco, J.M., Garin-Bastuji, B., Marín, C.M., Gerbier, G., Fanli, J., Jiménezde-Bagués, M.P., Cau, C., 1994. Efficacy of different Rose Bengal and complement fixation antigens for the diagnosis of Brucella melitensis infection in sheep and goats. Veterinary Record 134, 4154
13. Brown, S.L., Klein, G.C., McKinney, F.T., Jones, W.L., 1981. Safranin O-stained antigen microagglutination test for detection of Brucella antibodies. Journal of Clinical Microbiology 13, 398-400. Darwesh, M., Benkirane, A., 200 Field investigations of brucellosis in cattle and small ruminants in Syria, 1990-1996. Review Scientific in Technique 20, 769-775.
14. Dohoo, I.R., Wright, P.F., Ruckerbauer, G.M., Samagh, B.S., Robertson, F.J., Forbes, L.B., 19 A comparison of five serological tests for bovine brucellosis. Canadian Journal of Veterinary Research 50 (4), 485-493. Flad, S., 1983. Some observations on the use of Rose Bengal Plate, tube agglutination, heat inactivation and Rivanol tests in caprine brucellosis. Tropical Veterinary Medicine 1, 49
15. Gargouri, N., Walke, H., Belbeisi, A., Hadadin, A., Salah, S., Ellis, A., 2009. Estimated burden of human Salmonella, Shigella, and Brucella infections in Jordan, 2003-2004. Foodborne Pathogens and Disease 6 (4), 481-486.
16. Garin-Bastuji, B., Hummel, N., Gerbier, G., Cau, S., Pouillot, R., DaCosta, M., Fontaine, J.J., 19 Nonspecific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica O: Veterinary Microbiology 66, 223-233. Godfroid, J., Bosman, P.P., Herr, S., Bishop, G.C., 200 Bovine Brucellosis. Infectious In: Coetzer JAW, Thompson G, Tustin RC (eds.): Diseases of Livestock. 3rd edition. Oxford University Press, South Africa. 1510-1512.
17. Gwida, M., AlDS, M.F., Rosler, U., Neubauer, H., Tomaso, H., 2010. Brucellosis-regionally emerging zoonotic disease. Croatian Medical Journal 51 (4), 289-295.
18. Heydari, F., Ozaffari, N.A., Tukmechi, A., 2008. A comparison of standard seroagglutination tests and ELISA for diagnosis of brucellosis in west Azerbaijan province, Iran. Research Journal of Biological Sciences 3 (12), 1460-1462.
19. Hugh-Jones, M.E., 2000. Zoonoses, Recognition, Control and Prevention. In: Hugh-Jones ME, Hubbert WT, Hagstad HV (eds.): A Blackwell Publishing Company. Iowa State Press, first ed. pp. 7-27.
20. Khan, H.M.R., Khan, I., 2009. Seroprevalence of brucellosis in animals in district kohat NWFP and comparison of two serological tests. Pakistan Journal of Science 61 (4), 242-243.
21. Kumi-Diaka, J., Bale, J.O., Ogwu, D., Osori, D., 19 Effect of Brucella abortus infection on spermatogenesis in three Zebu bulls (Bos indicus). A case report. Theriogenology 14, 167-1 Maadi, H., Moharamnejad, M., Haghi, M., 2011. Prevalence of brucellosis in cattle in Urmia, Iran. Pakistan Veterinary Journal 31 (1), 81-82.
22. Mai, H.M., Irons, P.C., Kabir, J., Thompson, P.N., 20 A large seroprevalence survey of brucellosis in cattle herds under diverse production systems in northern Nigeria. BMC Veterinary Research 8, 144-149. McDermott, J.J., Arimi, S.M., 2002. Brucellosis in sub Saharan Africa: Epidemiology, control and impact, Veterinary Microbiology 90 (1-4), 1111
23. Mekonnen, H., Kalayou, S., Kyule, M., 2010. Serological survey of bovine brucellosis in barka and arado breeds of western tigray, Ethiopia. Preventive Veterinary Medicine 94 (12), 28-35.
24. Memish, Z., 2001. Brucellosis control in Saudi Arabia: prospects and challenges. Journal of Chemotherapy 13 (1), 11-17.
25. Moyer, N.P., Evins, G.M., Pigott, N.E., Hudson, J.D., Farshy, C.E., Feeley, J.C., Hausler, W.J., 1987. Comparison of serologic screening tests for brucellosis. Journal of Clinical Microbiology 25, 1969-1972.
26. Muma, J.B., Samui, K.L., Siamudaala, V.M., Oloya, J., Matope, G., Omer, M.K., Munyeme, M., Mubita, C., Skjerve, E., 2006. Prevalence of antibodies to Brucella spp. and individual risk factors of infection in traditional cattle, goats and sheep reared in livestock-wildlife interface areas of Zambia. Tropical Animal Health and Production 38, 195-206.
27. Nicoletti, P., 1980. The epidemiology of bovine brucellosis. Advances in Veterinary Science & Comparative Medicine 24, 69-98.
28. Nielsen, K., 2002. Diagnosis of brucellosis by serology. Veterinary Microbiology 90, 447-459.
29. Nikokar, I., Hosseinpour, M., Asmar, M., pirmohbatei, S., Hakeimei, F., Razavei, M.T., 20 Seroprevalence of Brucellosis among high risk individuals in Guilan, Iran. Journal of Research in Medical Sciences 16 (10), 136613
30. Ocholi, R.A., Kwaga, J.K.P., Ajogi, I., Bale, J.O., 200 Phenotypic characterization of Brucella strains isolated from livestock in Nigeria. Veterinary Microbiology 103, 47-53. OIE 2008. Bovine Brucellosis in Manual of Standards for Diagnostic Tests and Vaccines. 6 th ed. Paris, France. 328-345.
31. Otlu, S., Sahin, M., Atabay, H.I., Unver, A., 2008. Serological investigations of brucellosis in cattle, farmers and veterinarians in the Kars district of Turkey. Acta Veterinaria Brno 77, 117-1
32. Pappas, G., Markoula, S., Seitarids, S., Akritidis, N., Tsianos, T., 2005. Brucellosis as a cause of carpal tunnel syndrome. Annals of the Rheumatic Diseases 64, 792-793.
33. Poester, F.P., Goncalves, V.S.P., Lage, A.P., 2002. Brucellosis in Brazil. Veterinary Microbiology 90, 55-62.
34. Poester, F.P., Nielsen, K., Samartino, L.E., Yu, W.L., 2010. Diagnosis of brucellosis. The Open Veterinary Science Journal 4, 46-60.
35. Rahman, M.S., Faruk, M.O., Her, M., Kim, J.Y., Kang, S.I., Jung, S.C., 2011. Prevalence of brucellosis in ruminants in Bangladesh. Veterinarni Medicina 56 (8), 379-385.
36. Refai, M., 2002. Incidence and control of brucellosis in the Near East Region. Veterinary Microbiology 90, 81-110.
37. Reviriego, F.J., Moreno, M.A., Domínguez, L., 2000. Risk factors for brucellosis seroprevalence of sheep and goat flocks in Spain. Preventive Veterinary Medicine 44, 167-173.
38. Samaha, H., Al-Rowaily, M., Khoudair, R.M., Ashour, H.M., 2008. Multicenter study of brucellosis in Egypt. Emerging Infectious Diseases Journal 14 (12), 1916-1918.
39. Sareyyüpoğlu, B., Cantekin, Z., Müştak, H.K., 2010. Investigation of Brucella antibodies in bovine sera by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanolmicroagglutination (2-ME-MAT) test. Ankara Üniversitesi Veteriner Fakültesi Dergisi 57, 157-1
40. Shafee, M., Rabbani, M., Sheikh, A.A., Ahmad, M.D., Razzaq, A., 2011. Prevalence of bovine brucellosis in organized dairy farms, using milk ELISA, in Quetta City, Balochistan, Pakistan. SAGE-Hindawi Access to Research Veterina 1, 1Shareef, J.M., 2006. A review of serological investigations of brucellosis among farm animals and humans in Northern provinces of Iraq (1974-2004). Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health 53 (1), 38-40.
41. Sharma, V.D., Sethi, M.S., Yadav, M.P., Dube, D.C., 19 Sero-epidemiologic investigations on brucellosis in the states of Uttar Pradesh (U.P.) and Delhi (India). International Journal of Zoonoses 6, 75-81. Shimi, A., 1998. Veterinary bacteriology and bacterial disease. First ed. Jahad Publication Institute Iran. pp. 307-334.
42. Silva, I., Dangolla, A., Kulachelvy, K., 2000. Seroepidemiology of Brucella abortus infection in bovids in Sri Lanka. Preventive Veterinary Medicine 46, 51-59.
43. Zowghi, E., Ebadi, A., Mohseni, B., 1990. Isolation of Brucella organisms from the milk of seronegative cows. Review Scientific Technique 9, 1175-1178.